M-CSFR/CD115 Antibody (6B9B9) - BSA Free

Western Blot: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Analysis using CSF1R mAb against HEK293 (1) and CSF1R (AA: 344-497)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Analysis of HepG2 cells using CSF1R mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Analysis of esophageal tissues using CSF1R mouse mAb with DAB staining.

Western Blot: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Analysis using CSF1R mAb against human CSF1R (AA: 344-497) recombinant protein. (Expected MW is 43.3 kDa)

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Analysis of pancreas tissues using CSF1R mouse mAb with DAB staining.

ELISA: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 6B9B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

M-CSFR/CD115 Antibody (6B9B9) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human M-CSFR/CD115 (AA: 344-497) expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSF1R
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 108 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for M-CSFR/CD115 Antibody (6B9B9) - BSA Free

• CD115 antigen
• CD115
• c-fms
• colony stimulating factor 1 receptor
• CSF1R
• CSF-1-R
• CSFR
• EC 2.7.10.1
• FMS proto-oncogene
• FMSFIM2
• macrophage colony stimulating factor I receptor
• macrophage colony-stimulating factor 1 receptor
• McDonough feline sarcoma viral (v-fms) oncogene homolog
• M-CSF R
• MCSFR
• M-CSFR
• Proto-oncogene c-Fms

Background

MCSF Receptor is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a member of the CSF1/PDGF receptor family of tyrosine protein kinases and contains 5 immunoglobulin like C2 type domains. CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Mutations in this gene have been associated with a predisposition to myeloid malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for M-CSFR/CD115 Antibody (NBP2-37292)(1)

Publication using NBP2-37292

• FranzE E, Di Grazia A, Sica GS et al. Interleukin-34 Enhances the Tumor Promoting Function of Colorectal Cancer-Associated Fibroblasts Cancers (Basel) 2020-11-27 [PMID: 33260828] (ICC/IF, Human)

FAQs for M-CSFR/CD115 Antibody (NBP2-37292). (Showing 1 - 1 of 1 FAQ).

1. We need an antibody for immunofluorescence staining of macrophages for CSF-1 receptor.
   • It looks like the cat# NBP2-37292 is the only CSF Receptor that has been validated in ICC/IF.

Bioinformatics

• Gene Symbol: CSF1R