Podoplanin Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) followed by HRP-conjugated ...read more
Podoplanin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) at 15 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Podoplanin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Podoplanin
Gln21-Lys133
Accession # Q546R8
Specificity
Detects mouse Podoplanin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Podoplanin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDPN
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF3244 in the following applications:

Publications
Read Publications using
AF3244 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Podoplanin Antibody [Unconjugated]

  • 36-KD
  • Aggrus
  • Gp36
  • Gp38
  • GP40
  • HT1A-1
  • hT1alpha-1
  • hT1alpha-2
  • lung type I cell membrane associated glycoprotein
  • lung type-I cell membrane-associated glycoprotein (T1A-2)
  • OTS 8
  • OTS8
  • PA2.26 antigen
  • PDPN
  • Podoplanin
  • RANDAM-2
  • T1A
  • T1A-2
  • T1-alpha

Background

Podoplanin, also known as T1 alpha (T1 alpha ), is a mucin type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. In addition, non-endothelial cells in numerous normal tissues also express the protein. Within the region used as the immunogen, mouse Podoplanin shares 73.5% and 29% amino acid sequence homology with rat and human Podoplanin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Podoplanin Antibody (AF3244)(44)

We have publications tested in 4 confirmed species: Human, Mouse, Canine, Transgenic Mouse.

We have publications tested in 8 applications: Flow Cytometry, ICC, IF, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(2)
IF
(2)
IHC
(17)
IHC-Fr
(10)
IHC-P
(8)
Neutralization
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(2)
Mouse
(41)
Canine
(1)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 44. Show All 44 Publications.
Publications using AF3244 Applications Species
J Suzuki, Y Shimizu, T Hayashi, Y Che, Z Pu, K Tsuzuki, S Narita, R Shibata, I Ishii, JW Calvert, T Murohara Hydrogen Sulfide Attenuates Lymphedema Via the Induction of Lymphangiogenesis Through a PI3K/Akt-Dependent Mechanism Journal of the American Heart Association, 2022-10-26;11(21):e026889. 2022-10-26 [PMID: 36285798] (IHC, Mouse) IHC Mouse
Q Li, R Wang, Z Zhang, H Wang, X Lu, J Zhang, AP Kong, XY Tian, HF Chan, AC Chung, JC Cheng, Q Jiang, WY Lee Sirt3 mediates the benefits of exercise on bone in aged mice Cell Death and Differentiation, 2022-09-24;0(0):. 2022-09-24 [PMID: 36153410] (IHC, Mouse) IHC Mouse
H Moriyama, J Endo, M Kataoka, Y Shimanaka, N Kono, Y Sugiura, S Goto, H Kitakata, T Hiraide, N Yoshida, S Isobe, T Yamamoto, K Shirakawa, A Anzai, Y Katsumata, M Suematsu, K Kosaki, K Fukuda, H Arai, M Sano Omega-3 fatty acid epoxides produced by PAF-AH2 in mast cells regulate pulmonary vascular remodeling Nature Communications, 2022-05-31;13(1):3013. 2022-05-31 [PMID: 35641514] (IHC, Mouse) IHC Mouse
TY Kim, S Kim, Y Kim, YS Lee, S Lee, SH Lee, MN Kweon A High-Fat Diet Activates the BAs-FXR Axis and Triggers Cancer-Associated Fibroblast Properties in the Colon Cellular and Molecular Gastroenterology and Hepatology, 2021-12-29;0(0):. 2021-12-29 [PMID: 34971821] (IHC, Mouse) IHC Mouse
G Baranwal, HA Creed, LM Black, A Auger, AM Quach, R Vegiraju, HE Eckenrode, A Agarwal, JM Rutkowski Expanded renal lymphatics improve recovery following kidney injury Physiological Reports, 2021-11-01;9(22):e15094. 2021-11-01 [PMID: 34806312] (IHC, Mouse) IHC Mouse
L Johnson Ch, H Lahlou, C Steinacher, S Assou, Y Messat, J Dudás, A Edge, B Crespo, M Crosier, C Sergi, A Schrott-Fi, A Zine Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs International Journal of Molecular Sciences, 2021-10-07;22(19):. 2021-10-07 [PMID: 34639189] (IF, Mouse) IF Mouse
D Chanda, M Rehan, SR Smith, KG Dsouza, Y Wang, K Bernard, D Kurundkar, V Memula, K Kojima, JA Mobley, GA Benavides, V Darley-Usm, YI Kim, JW Zmijewski, JS Deshane, S De Langhe, VJ Thannickal Mesenchymal stromal cell aging impairs the self-organizing capacity of lung alveolar epithelial stem cells Elife, 2021-09-16;10(0):. 2021-09-16 [PMID: 34528872] (IHC, Mouse) IHC Mouse
D Sz?ke, G Kovács, É Kemecsei, L Bálint, K Szoták-Ajt, P Aradi, A Styevkóné, BL Mui, YK Tam, TD Madden, K Karikó, RP Kataru, MJ Hope, D Weissman, BJ Mehrara, N Pardi, Z Jakus Nucleoside-modified VEGFC mRNA induces organ-specific lymphatic growth and reverses experimental lymphedema Nature Communications, 2021-06-08;12(1):3460. 2021-06-08 [PMID: 34103491] (IHC, Mouse) IHC Mouse
R Luo, Y Cheng, D Chang, T Liu, L Liu, G Pei, N Zhang, Z Wang, K Guo, W Chen, M Li, L Fan, C Zhang, Y Li, W Dai, M Zuo, Y Xu, Y Yao, S Ge, G Xu Tertiary lymphoid organs are associated with the progression of kidney damage and regulated by interleukin-17A Theranostics, 2021-01-01;11(1):117-131. 2021-01-01 [PMID: 33391465] (IHC, Human) IHC Human
J Weber, S Rajan, C Schremmer, YK Chao, G Krasteva-C, M Kannler, AÖ Yildirim, M Brosien, J Schredelse, N Weissmann, C Grimm, T Gudermann, A Dietrich TRPV4 channels are essential for alveolar epithelial barrier function as protection from lung edema JCI Insight, 2020-10-15;0(0):. 2020-10-15 [PMID: 32931478] (Western Blot, Mouse) Western Blot Mouse
Show All 44 Publications.

Review for Podoplanin Antibody (AF3244) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF3244:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous -
ICC Mouse 01/07/2015
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Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 23892627
SpeciesMouse

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Blogs on Podoplanin.

Spheroids vs. Organoids: Which 3D Cell Culture Model is Best for You?
By Jennifer Jones, M.S.Spheroids and organoids are two words that, like “butter” and “margarine”, are often referred to interchangeably but have distinct meanings. The progression and adopt...  Read full blog post.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein)
Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with ...  Read full blog post.

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Anonymous -
01/07/2015
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol PDPN
Uniprot