Podoplanin Antibody [Unconjugated]

Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Podoplanin at approximately 35-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Podoplanin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Podoplanin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Podoplanin
  Gln21-Lys133
  Accession # Q546R8
• Specificity: Detects mouse Podoplanin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Podoplanin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDPN
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Podoplanin Antibody [Unconjugated]

• 36-KD
• Aggrus
• Gp36
• Gp38
• GP40
• HT1A-1
• hT1alpha-1
• hT1alpha-2
• lung type I cell membrane associated glycoprotein
• lung type-I cell membrane-associated glycoprotein (T1A-2)
• OTS 8
• OTS8
• PA2.26 antigen
• PDPN
• Podoplanin
• RANDAM-2
• T1A
• T1A-2
• T1-alpha

Background

Podoplanin, also known as T1 alpha (T1 alpha ), is a mucin type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. In addition, non-endothelial cells in numerous normal tissues also express the protein. Within the region used as the immunogen, mouse Podoplanin shares 73.5% and 29% amino acid sequence homology with rat and human Podoplanin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Podoplanin Antibody (AF3244)(44)

Publications using AF3244

• J Suzuki, Y Shimizu, T Hayashi, Y Che, Z Pu, K Tsuzuki, S Narita, R Shibata, I Ishii, JW Calvert, T Murohara Hydrogen Sulfide Attenuates Lymphedema Via the Induction of Lymphangiogenesis Through a PI3K/Akt-Dependent Mechanism Journal of the American Heart Association, 2022-10-26;11(21):e026889. 2022-10-26 [PMID: 36285798] (IHC, Mouse)
• Q Li, R Wang, Z Zhang, H Wang, X Lu, J Zhang, AP Kong, XY Tian, HF Chan, AC Chung, JC Cheng, Q Jiang, WY Lee Sirt3 mediates the benefits of exercise on bone in aged mice Cell Death and Differentiation, 2022-09-24;0(0):. 2022-09-24 [PMID: 36153410] (IHC, Mouse)
• H Moriyama, J Endo, M Kataoka, Y Shimanaka, N Kono, Y Sugiura, S Goto, H Kitakata, T Hiraide, N Yoshida, S Isobe, T Yamamoto, K Shirakawa, A Anzai, Y Katsumata, M Suematsu, K Kosaki, K Fukuda, H Arai, M Sano Omega-3 fatty acid epoxides produced by PAF-AH2 in mast cells regulate pulmonary vascular remodeling Nature Communications, 2022-05-31;13(1):3013. 2022-05-31 [PMID: 35641514] (IHC, Mouse)
• TY Kim, S Kim, Y Kim, YS Lee, S Lee, SH Lee, MN Kweon A High-Fat Diet Activates the BAs-FXR Axis and Triggers Cancer-Associated Fibroblast Properties in the Colon Cellular and Molecular Gastroenterology and Hepatology, 2021-12-29;0(0):. 2021-12-29 [PMID: 34971821] (IHC, Mouse)
• G Baranwal, HA Creed, LM Black, A Auger, AM Quach, R Vegiraju, HE Eckenrode, A Agarwal, JM Rutkowski Expanded renal lymphatics improve recovery following kidney injury Physiological Reports, 2021-11-01;9(22):e15094. 2021-11-01 [PMID: 34806312] (IHC, Mouse)
• L Johnson Ch, H Lahlou, C Steinacher, S Assou, Y Messat, J Dudás, A Edge, B Crespo, M Crosier, C Sergi, A Schrott-Fi, A Zine Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs International Journal of Molecular Sciences, 2021-10-07;22(19):. 2021-10-07 [PMID: 34639189] (IF, Mouse)
• D Chanda, M Rehan, SR Smith, KG Dsouza, Y Wang, K Bernard, D Kurundkar, V Memula, K Kojima, JA Mobley, GA Benavides, V Darley-Usm, YI Kim, JW Zmijewski, JS Deshane, S De Langhe, VJ Thannickal Mesenchymal stromal cell aging impairs the self-organizing capacity of lung alveolar epithelial stem cells Elife, 2021-09-16;10(0):. 2021-09-16 [PMID: 34528872] (IHC, Mouse)
• D Sz?ke, G Kovács, É Kemecsei, L Bálint, K Szoták-Ajt, P Aradi, A Styevkóné, BL Mui, YK Tam, TD Madden, K Karikó, RP Kataru, MJ Hope, D Weissman, BJ Mehrara, N Pardi, Z Jakus Nucleoside-modified VEGFC mRNA induces organ-specific lymphatic growth and reverses experimental lymphedema Nature Communications, 2021-06-08;12(1):3460. 2021-06-08 [PMID: 34103491] (IHC, Mouse)
• R Luo, Y Cheng, D Chang, T Liu, L Liu, G Pei, N Zhang, Z Wang, K Guo, W Chen, M Li, L Fan, C Zhang, Y Li, W Dai, M Zuo, Y Xu, Y Yao, S Ge, G Xu Tertiary lymphoid organs are associated with the progression of kidney damage and regulated by interleukin-17A Theranostics, 2021-01-01;11(1):117-131. 2021-01-01 [PMID: 33391465] (IHC, Human)
• J Weber, S Rajan, C Schremmer, YK Chao, G Krasteva-C, M Kannler, AÖ Yildirim, M Brosien, J Schredelse, N Weissmann, C Grimm, T Gudermann, A Dietrich TRPV4 channels are essential for alveolar epithelial barrier function as protection from lung edema JCI Insight, 2020-10-15;0(0):. 2020-10-15 [PMID: 32931478] (Western Blot, Mouse)
• M Strunz, LM Simon, M Ansari, JJ Kathiriya, I Angelidis, CH Mayr, G Tsidiridis, M Lange, LF Mattner, M Yee, P Ogar, A Sengupta, I Kukhtevich, R Schneider, Z Zhao, C Voss, T Stoeger, JHL Neumann, A Hilgendorf, J Behr, M O'Reilly, M Lehmann, G Burgstalle, M Königshoff, HA Chapman, FJ Theis, HB Schiller Alveolar regeneration through a Krt8+ transitional stem cell state that persists in human lung fibrosis Nat Commun, 2020-07-16;11(1):3559. 2020-07-16 [PMID: 32678092] (IHC, Western Blot, Mouse)
• M Sesarti?, K Ikenberg, SY Yoon, M Detmar Keratinocyte-Expressed Podoplanin is Dispensable for Multi-Step Skin Carcinogenesis Cells, 2020-06-24;9(6):. 2020-06-24 [PMID: 32599908] (IHC, Transgenic Mouse)
• X Li, J Zhao, V Kasinath, M Uehara, L Jiang, N Banouni, MM McGrath, T Ichimura, P Fiorina, DR Lemos, SR Shin, CF Ware, JS Bromberg, R Abdi Lymph node fibroblastic reticular cells deposit fibrosis-associated collagen following organ transplantation J. Clin. Invest., 2020-08-03;0(0):. 2020-08-03 [PMID: 32597832] (IF, Mouse)
• A Chakrabort, CK Scogin, K Rizwan, TS Morley, JM Rutkowski Characterizing Lymphangiogenesis and Concurrent Inflammation in Adipose Tissue in Response to VEGF-D Front Physiol, 2020-04-22;11(0):363. 2020-04-22 [PMID: 32390866] (IHC-P, Mouse)
• KA Staines, E Ikpegbu, AE Törnqvist, S Dillon, B Javaheri, AK Amin, DN Clements, DJ Buttle, AA Pitsillide, C Farquharso Conditional deletion of E11/podoplanin in bone protects against load-induced osteoarthritis BMC Musculoskelet Disord, 2019-07-27;20(1):344. 2019-07-27 [PMID: 31351471] (IHC-P, Canine, Human, Mouse)
• SY Yoon, LC Dieterich, S Karaman, ST Proulx, SB Bachmann, C Sciaroni, M Detmar An important role of cutaneous lymphatic vessels in coordinating and promoting anagen hair follicle growth PLoS ONE, 2019-07-25;14(7):e0220341. 2019-07-25 [PMID: 31344105] (IHC, Mouse)
• SY Yoon, LC Dieterich, C Tacconi, M Sesartic, Y He, L Brunner, O Kwon, M Detmar An important role of podoplanin in hair follicle growth PLoS ONE, 2019-07-23;14(7):e0219938. 2019-07-23 [PMID: 31335913] (IHC-Fr, Mouse)
• P Chen, S Wang, KS Janardhan, RL Zemans, W Deng, P Karmaus, S Shen, M Sunday, LG Que, MB Fessler, XP Zhong Efficient CD4Cre-Mediated Conditional KRas Expression in Alveolar Macrophages and Alveolar Epithelial Cells Causes Fatal Hyperproliferative Pneumonitis J. Immunol., 2019-07-17;0(0):. 2019-07-17 [PMID: 31315887] (IHC-P, Mouse)
• H Tanaka, K Horioka, M Yamamoto, M Asari, K Okuda, K Yamazaki, K Shimizu, K Ogawa Overproduction of thrombopoietin by BRAFV600E-mutated mouse hepatocytes and contribution of thrombopoietin to hepatocarcinogenesis Cancer Sci., 2019-07-30;110(9):2748-2759. 2019-07-30 [PMID: 31301081] (IHC-P, Mouse)
• X Li, A Bean, M Uehara, N Banouni, M Ben Nasr, V Kasinath, L Jiang, P Fiorina, R Abdi Immune heterogeneity of head and tail pancreatic lymph nodes in non-obese diabetic mice Sci Rep, 2019-07-05;9(1):9778. 2019-07-05 [PMID: 31278331] (IHC-Fr, Mouse)
• M Uehara, X Li, A Sheikhi, N Zandi, B Walker, B Saleh, N Banouni, L Jiang, F Ordikhani, L Dai, M Yonar, I Vohra, V Kasinath, DP Orgill, A Khademhoss, N Annabi, R Abdi Anti-IL-6 eluting immunomodulatory biomaterials prolong skin allograft survival Sci Rep, 2019-04-25;9(1):6535. 2019-04-25 [PMID: 31024011] (IHC-Fr, Mouse)
• L Liu, L Zhao, Y Yang, J Gao, C Hu, B Guo, B Zhu Cytotoxic chemotherapy reduces T cell trafficking to the spleen by downregulating the expression of C-C motif chemokine ligand 21 and C-C motif chemokine ligand 19 Oncol Lett, 2018-08-09;16(4):5013-5019. 2018-08-09 [PMID: 30250567]
• Q Ma, LC Dieterich, K Ikenberg, SB Bachmann, J Mangana, ST Proulx, VC Amann, MP Levesque, R Dummer, P Baluk, DM McDonald, M Detmar Unexpected contribution of lymphatic vessels to promotion of distant metastatic tumor spread Sci Adv, 2018-08-08;4(8):eaat4758. 2018-08-08 [PMID: 30101193] (IHC, Mouse)
• OH Maarouf, M Uehara, V Kasinath, Z Solhjou, N Banouni, B Bahmani, L Jiang, OA Yilmam, I Guleria, SB Lovitch, JL Grogan, P Fiorina, PT Sage, JS Bromberg, MM McGrath, R Abdi Repetitive ischemic injuries to the kidneys result in lymph node fibrosis and impaired healing JCI Insight, 2018-07-12;3(13):. 2018-07-12 [PMID: 29997302] (Flow Cytometry, Mouse)
• AR Harris, MJ Perez, JM Munson Docetaxel facilitates lymphatic-tumor crosstalk to promote lymphangiogenesis and cancer progression BMC Cancer, 2018-07-06;18(1):718. 2018-07-06 [PMID: 29976154] (IHC-Fr, Mouse)
• A Song, L Zhu, G Gorantla, O Berdysz, SA Amici, M Guerau-de-, KM Madalena, JK Lerch, X Liu, N Quan Salient type 1 interleukin 1 receptor expression in peripheral non-immune cells Sci Rep, 2018-01-15;8(1):723. 2018-01-15 [PMID: 29335509] (IHC-Fr, Mouse)
• BF Westendorp, NVJA Büller, ON Karpus, WA van Dop, J Koster, R Versteeg, PJ Koelink, CY Snel, S Meisner, JJTH Roelofs, A Uhmann, E Ver Loren, J Heijmans, H Hahn, V Muncan, ME Wildenberg, GR van den Br Indian Hedgehog Suppresses a Stromal Cell-Driven Intestinal Immune Response Cell Mol Gastroenterol Hepatol, 2017-09-05;5(1):67-82.e1. 2017-09-05 [PMID: 29276753] (IHC-P, Mouse)
• MW Henderson, JH Madenspach, GS Whitehead, SY Thomas, JJ Aloor, KM Gowdy, MB Fessler Effects of Orally Ingested Arsenic on Respiratory Epithelial Permeability to Bacteria and Small Molecules in Mice Environ. Health Perspect., 2017-09-28;125(9):097024. 2017-09-28 [PMID: 28960179] (Western Blot, Mouse)
• LA Johnson, S Banerji, W Lawrance, U Gileadi, G Prota, KA Holder, YM Roshorm, T Hanke, V Cerundolo, NW Gale, DG Jackson Dendritic cells enter lymph vessels by hyaluronan-mediated docking to the endothelial receptor LYVE-1 Nat. Immunol., 2017-05-15;0(0):. 2017-05-15 [PMID: 28504698] (IHC, Mouse)
• CSE Tan, C Lawler, PG Stevenson CD8+ T cell evasion mandates CD4+ T cell control of chronic gamma-herpesvirus infection PLoS Pathog., 2017-04-10;13(4):e1006311. 2017-04-10 [PMID: 28394921] (IHC, Mouse)
• D Zheng, BS Soh, L Yin, G Hu, Q Chen, H Choi, J Han, VT Chow, J Chen Differentiation of Club Cells to Alveolar Epithelial Cells In Vitro Sci Rep, 2017-01-27;7(0):41661. 2017-01-27 [PMID: 28128362] (ICC, Mouse)
• Hoeke A Baarsma Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD J. Exp. Med, 2016-12-15;0(0):. 2016-12-15 [PMID: 27979969] (ICC, Mouse)
• Michael Detmar Postnatal Deletion of Podoplanin in Lymphatic Endothelium Results in Blood Filling of the Lymphatic System and Impairs Dendritic Cell Migration to Lymph Nodes Arterioscler. Thromb. Vasc. Biol., 2016-11-03;0(0):. 2016-11-03 [PMID: 27810998] (IHC-Fr, Mouse)
• Type I Interferons Direct Gammaherpesvirus Host Colonization PLoS Pathog, 2016-05-25;12(5):e1005654. 2016-05-25 [PMID: 27223694] (IHC, Mouse)
• E Hintermann, M Bayer, J Ehser, M Aurrand-Li, JM Pfeilschif, BA Imhof, U Christen Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis Cell Adh Migr, 2016-04-25;10(4):419-33. 2016-04-25 [PMID: 27111582] (IHC-Fr, Mouse)
• Yin L, Zheng D, Limmon G, Leung N, Xu S, Rajapakse J, Yu H, Chow V, Chen J Aging exacerbates damage and delays repair of alveolar epithelia following influenza viral pneumonia. Respir Res, 2014-09-30;15(0):116. 2014-09-30 [PMID: 25265939] (IHC-P, Mouse)
• Lenoir B, Wagner D, Blacher S, Sala-Newby G, Newby A, Noel A, Devaux Y Effects of adenosine on lymphangiogenesis. PLoS ONE, 2014-03-20;9(3):e92715. 2014-03-20 [PMID: 24651845] (IHC, Mouse)
• Sturrock , Anne, Mir-Kasimov , Mustafa, Baker , Jessica, Rowley , Jesse, Paine , Robert 3 Key role of microRNA in the regulation of granulocyte macrophage colony-stimulating factor expression in murine alveolar epithelial cells during oxidative stress. J Biol Chem, 2013-12-26;289(7):4095-105. 2013-12-26 [PMID: 24371146] (IHC, Mouse)
• Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F Distinct fibroblast lineages determine dermal architecture in skin development and repair. Nature, 2013-12-12;504(7479):277-81. 2013-12-12 [PMID: 24336287] (IHC-Fr, Mouse)
• Zheng , Dahai, Limmon , Gino V, Yin , Lu, Leung , Nicola H, Yu , Hanry, Chow , Vincent, Chen , Jianzhu Regeneration of alveolar type I and II cells from Scgb1a1-expressing cells following severe pulmonary damage induced by bleomycin and influenza. PLoS ONE, 2012-10-31;7(10):e48451. 2012-10-31 [PMID: 23119022] (IHC-P, Mouse)
• Impaired humoral immunity and tolerance in K14-VEGFR-3-Ig mice that lack dermal lymphatic drainage. J. Immunol., 2012-07-27;189(5):2181-90. 2012-07-27 [PMID: 22844119] (IHC-Fr, Mouse)
• Peters A, Pitcher LA, Sullivan JM, Mitsdoerffer M, Acton SE, Franz B, Wucherpfennig K, Turley S, Carroll MC, Sobel RA, Bettelli E, Kuchroo VK Th17 cells induce ectopic lymphoid follicles in central nervous system tissue inflammation. Immunity, 2011-12-15;35(6):986-96. 2011-12-15 [PMID: 22177922] (Neutralization, Mouse)
• Zhu D, Mackenzie NC, Millan JL, Farquharson C, MacRae VE The Appearance and Modulation of Osteocyte Marker Expression during Calcification of Vascular Smooth Muscle Cells. PLoS ONE, 2011-05-17;6(5):e19595. 2011-05-17 [PMID: 21611184] (IHC-P, Western Blot, Mouse)
• Xu Y, Yuan L, Mak J, Pardanaud L, Caunt M, Kasman I, Larrivee B, Del Toro R, Suchting S, Medvinsky A, Silva J, Yang J, Thomas JL, Koch AW, Alitalo K, Eichmann A, Bagri A Neuropilin-2 mediates VEGF-C-induced lymphatic sprouting together with VEGFR3. J. Cell Biol., 2010-01-11;188(1):115-30. 2010-01-11 [PMID: 20065093] (IHC-Fr, Mouse)

Review for Podoplanin Antibody (AF3244) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous - ICC Mouse 01/07/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: See PMID 23892627
• Species: Mouse

Bioinformatics

• Gene Symbol: PDPN
• Uniprot:
  • Q546R8()