Podoplanin Antibody [Unconjugated]

Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Podoplanin at approximately 35-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Podoplanin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western shows lysates of mouse lung, loaded at 0.5 mg/ml. A specific band was detected for Podoplanin at approximately 60 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Mfn1/2i delta AEC2 mice develop spontaneous lung fibrosis. a Schema demonstrating the generation of mice with AEC2 cell-specific tamoxifen-inducible deletion of Mfn1/2. SftpcCreERT2+/+ or SftpcCreERT2+/− mice were used as controls. b Genotyping of CD45(-)EpCAM(+) cells isolated from Mfn1/2i delta AEC2 mice (n = 3 mice; lane 1 to lane 3 serves as the positive control). c Representative immunoblots of AEC2 cell lysates obtained 6 weeks after tamoxifen-induced deletion, showing decreased protein levels of both MFN1 and MFN2 in the Mfn1/2−/− AEC2 cells (n = 3 mice per group). d Representative TEM images (upper row, ×12,000; lower row, ×50,000) show mitochondrial ultrastructural changes in SftpcCreERT2+/− and Mfn1/2−/− AEC2 cells (n = 3 mice per group) with disrupted cristae marked with white arrowheads (scale bar, upper row 2 μm, lower row 500 nm. e Kaplan–Meier survival curves of Mfn1/2i delta AEC2 (n = 22) and SftpcCreERT2+/+ (n = 23) mice (p < 0.01 by log-rank test). f Representative Masson’s trichrome-stained lung sections (upper panel, ×100 magnification; lower panel, ×200 magnification) 17 weeks post tamoxifen-induced deletion (SftpcCreERT2+/+ mice n = 6; Mfn1/2i delta AEC2 mice n = 11; scale bar, upper panel 4 mm, lower panel 200 μm). g Representative IHC staining of vimentin, alpha-smooth muscle actin ( alpha -SMA), and collagen III (Col-III) (×200 magnification; n = 3 mice per group; scale bar 200 μm). h Representative immunofluorescent staining of 5x5 tiled confocal images (using ×40 objective) of frozen murine lung sections stained for podoplanin (green), surfactant protein-C (SP-C) (yellow), ER-TR7 (magenta), and Hoechst 33342 stain (blue) (n = 3 mice per group; scale bar 50 μm). i Representative immunofluorescence staining confocal images of podoplanin (green), SP-C (yellow), ER-TR7 (magenta) and Hoechst 33342 nuclear stain (blue) using lung sections of SftpcCreERT2+/−, Mfn1i delta AEC2 and Mfn2i delta AEC2 mice (n = 2 mice per group; scale bar 20 μm). Source data (c, e) are provided as a Source Data file Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31358769), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of VEGFCC156S on angiotensin II-induced cardiac dysfunction, cardiac lymphatics, and skin lymphatics.(A) Pharmacokinetic analysis of VEGFCC156S in mice (n = 2 per dose group). (B) Plasma VEGFC concentration measured by ELISA at euthanasia (n = 10–17/group). (C–S) Mice were infused with saline + BSA, angiotensin II + BSA, or angiotensin II + VEGFCC156S as described in Figure 2A. (C) Representative M-mode echocardiography images for each group. (D–G) Echocardiography parameters cardiac output (D), stroke volume (E), Left Ventricular Posterior Wall thickness at diastole (LVPWd) (F) and heart rate (G) are shown (n = 15–24/group). (H–J) Hearts were arrested in diastole, fixed, sectioned, and stained with wheat germ agglutinin (WGA). (H) Representative images of WGA stain for each group. Scale bar, 100 μm. (I) Cardiomyocyte size was assessed (n = 7–9/group). (J) Analysis of cell size variance coefficient from WGA-stained mouse heart sections (n = 21–27/group; a linear mixed model was used for statistics). (K–N) Protein lysates were prepared from mouse hearts and immunoblotted for podoplanin, p-Akt, Akt, p-Erk1/2, Erk1/2, and GAPDH. (K) Representative immunoblots. (L–N) Densitometric quantification of podoplanin (L), p-Akt to Akt ratio (M) and p-Erk to Erk ratio (N) (n = 7–9/group). (O) Representative images of the whole mount stain of lyve1-positive lymphatic vessels in ear skin for each group. Scale bar, 100 μm. (P) Quantification of lyve1-positive lymphatic vessel diameter in ear skin (n = 737–880 vessel/group; a linear mixed model was used for statistics). (Q) Ear skin samples were fixed, sectioned, and stained for lyve1(green) and DAPI (blue). Representative images of lyve1-positive lymphatic vessels in ear skin cross-section for each group. Scale bar, 200 μm. (R) Quantification of lyve1-positive lymphatic vessel density (normalized to total area) in ear skin (n = 6–8 animals/group, n = 6–8 sections/animal; a linear mixed model was used for statistics). Ctrl, control. AngII, angiotensin II. VEGFC, VEGFCc156s. Data are mean ± s.d. One-way ANOVA with Bonferroni posthoc was used for statistics for all figures except 2J, 2P and 2R (a linear mixed model was used for statistics). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, n.s. not significant. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33200983), licensed under a CC-BY license. Not internally tested by R&D Systems.

Carboplatin induces lymphangiogenesis in healthy tissues (A) Schematic of rat mesentery culture model. (B) Vehicle-treated lymphatic vessels from mesentery cultures stained with LYVE-1 (grey). (i) High magnification image of boxed area in (B). (C) Carboplatin-treated lymphatic vessels from mesentery cultures stained with LYVE-1 (grey). (ii) High magnification image of boxed area in (C) Scale bar=100µm. (D) Number of sprouts per lymphatic vessel area (n=3/group). (E) Lymphatic vessel density (podoplanin+ vessels per mm2 stroma) in whole mammary fat pads of healthy mice treated with systemic carboplatin (8 mg/kg/dose) or vehicle by IV(n=3-4/group). (F) Lymphatic vessel density measured in mammary fat pads of healthy mice 2 months after treatment with 3 doses of carboplatin or vehicle, (n=3/group). (G) Lymph nodes from healthy, tumor-naïve mice treated with vehicle and stained H&E. (H) LEC number in vehicle-treated and carboplatin-treated lymph nodes in vivo (n=6/group). (I) Representative images of lungs from mice treated with 3 doses of vehicle (left) and carboplatin (right). Podoplanin+ lymphatic vessels noted by arrowheads. (J) Lymphatic vessel density in stromal tissue of lungs of mice pre-treated with carboplatin (n=3/group). *p < 0.05, **p < 0.01, ****p < 0.001. Each data point represents one mouse. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35372032), licensed under a CC-BY license. Not internally tested by R&D Systems.

Podoplanin immunoreactivity in FFPE sections of mouse small intestine and lung. AF3244 was used at 125ng/mL and was left on tissue sections for 30m at room temperature. Formalin fixed paraffin embedded sections were deparaffinized and rehydrated. Sections then underwent heat induced epitope retrieval in a citrate-based solution for 20min in a food steamer. Endogenous peroxidase activity was blocked by incubating slides for 15min in tris buffered saline containing 3% (volume/volume) hydrogen peroxide. Tissue sections were then washed, blocked with normal horse serum for 20 min, and then incubated with the primary antibody (125ng/mL) for 30min at room temperature. Sections were then washed and incubated with a horse anti-goat HRP polymer for 30m at room temperature. Sections were washed and incubated with DAB chromogen for approximately 2.5min. Sections were then washed, counterstained with hematoxylin, dehydrated, cleared and mounted with a coverslip. Image from a verified customer review.

• Reactivity: Mu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Podoplanin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Podoplanin
  Gln21-Lys133
  Accession # Q546R8
• Specificity: Detects mouse Podoplanin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Podoplanin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDPN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Podoplanin Antibody [Unconjugated]

• 36-KD
• Aggrus
• Gp36
• Gp38
• GP40
• HT1A-1
• hT1alpha-1
• hT1alpha-2
• lung type I cell membrane associated glycoprotein
• lung type-I cell membrane-associated glycoprotein (T1A-2)
• OTS 8
• OTS8
• PA2.26 antigen
• PDPN
• Podoplanin
• RANDAM-2
• T1A
• T1A-2
• T1-alpha

Background

Podoplanin, also known as T1 alpha (T1 alpha ), is a mucin type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. In addition, non-endothelial cells in numerous normal tissues also express the protein. Within the region used as the immunogen, mouse Podoplanin shares 73.5% and 29% amino acid sequence homology with rat and human Podoplanin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Podoplanin Antibody (AF3244)(78)

Publications using AF3244

• Lauer, D;Magnin, CY;Kolly, LR;Wang, H;Brunner, M;Chabria, M;Cereghetti, GM;Gabry?, HS;Tanadini-Lang, S;Uldry, AC;Heller, M;Verleden, SE;Klein, K;Sarbu, AC;Funke-Chambour, M;Ebner, L;Distler, O;Maurer, B;Gote-Schniering, J; Radioproteomics stratifies molecular response to antifibrotic treatment in pulmonary fibrosis JCI insight 2024-07-16 [PMID: 39012714] (Immunohistochemistry, Mouse)
• Fernandes, R;Barbosa-Matos, C;Borges-Pereira, C;Carvalho, ALRT;Costa, S; Glycogen Synthase Kinase-3 Inhibition by CHIR99021 Promotes Alveolar Epithelial Cell Proliferation and Lung Regeneration in the Lipopolysaccharide-Induced Acute Lung Injury Mouse Model International journal of molecular sciences 2024-01-20 [PMID: 38279281] (Western Blot, Mouse)
• Singla B, Aithbathula RV, Pervaiz N et al. CD47 Activation by Thrombospondin-1 in Lymphatic Endothelial Cells Suppresses Lymphangiogenesis and Promotes Atherosclerosis Arteriosclerosis, thrombosis, and vascular biology 7/1/2023 [PMID: 37259865]
  
  Details:
  IHC Dilution: 1:100
• Xiao, Z;Todd, L;Huang, L;Noguera-Ortega, E;Lu, Z;Huang, L;Kopp, M;Li, Y;Pattada, N;Zhong, W;Guo, W;Scholler, J;Liousia, M;Assenmacher, CA;June, CH;Albelda, SM;Puré, E; Desmoplastic stroma restricts T cell extravasation and mediates immune exclusion and immunosuppression in solid tumors bioRxiv : the preprint server for biology 2023-08-07 [PMID: 37090547] (Immunohistochemistry, Mouse)
• J Suzuki, Y Shimizu, T Hayashi, Y Che, Z Pu, K Tsuzuki, S Narita, R Shibata, I Ishii, JW Calvert, T Murohara Hydrogen Sulfide Attenuates Lymphedema Via the Induction of Lymphangiogenesis Through a PI3K/Akt-Dependent Mechanism Journal of the American Heart Association, 2022-10-26;11(21):e026889. 2022-10-26 [PMID: 36285798] (IHC, Mouse)
• Q Li, R Wang, Z Zhang, H Wang, X Lu, J Zhang, AP Kong, XY Tian, HF Chan, AC Chung, JC Cheng, Q Jiang, WY Lee Sirt3 mediates the benefits of exercise on bone in aged mice Cell Death and Differentiation, 2022-09-24;0(0):. 2022-09-24 [PMID: 36153410] (IHC, Mouse)
• Yi Luan, Qianying Yuan, Qijun Wang, Susan Compton, Dianqing Wu, Wenwen Tang Pazopanib Is a Potential Treatment for Coronavirus-Induced Lung Injuries The Journal of Immunology 8/15/2022 [PMID: 35914834]
• Yi Luan, Qianying Yuan, Qijun Wang, Susan Compton, Dianqing Wu, Wenwen Tang Pazopanib Is a Potential Treatment for Coronavirus-Induced Lung Injuries The Journal of Immunology 2022-08-15 [PMID: 35914834] (Immunohistochemistry, Mouse)
• H Moriyama, J Endo, M Kataoka, Y Shimanaka, N Kono, Y Sugiura, S Goto, H Kitakata, T Hiraide, N Yoshida, S Isobe, T Yamamoto, K Shirakawa, A Anzai, Y Katsumata, M Suematsu, K Kosaki, K Fukuda, H Arai, M Sano Omega-3 fatty acid epoxides produced by PAF-AH2 in mast cells regulate pulmonary vascular remodeling Nature Communications, 2022-05-31;13(1):3013. 2022-05-31 [PMID: 35641514] (IHC, Mouse)
• Alexandra R. Harris, Savieay Esparza, Mohammad S. Azimi, Robert Cornelison, Francesca N. Azar, Danielle C. Llaneza et al. Platinum Chemotherapy Induces Lymphangiogenesis in Cancerous and Healthy Tissues That Can be Prevented With Adjuvant Anti-VEGFR3 Therapy Frontiers in Oncology 3/17/2022 [PMID: 35372032]
• G Günes Güns, TM Conlon, A Jeridi, R Kim, Z Ertüz, NJ Lang, M Ansari, M Novikova, D Jiang, M Strunz, M Gaianova, C Hollauer, C Gabriel, I Angelidis, S Doll, JC Pestoni, SL Edelmann, MS Kohlhepp, A Guillot, K Bassler, HP Van Eeckho, Ö Kayalar, N Konyalilar, T Kanashova, S Rodius, C Ballester-, CM Genes Robl, N Smirnova, M Rehberg, C Agarwal, I Krikki, B Piavaux, SE Verleden, B Vanaudenae, M Königshoff, G Dittmar, KR Bracke, JL Schultze, H Watz, O Eickelberg, T Stoeger, G Burgstalle, F Tacke, V Heissmeyer, Y Rinkevich, H Bayram, HB Schiller, M Conrad, R Schneider, AÖ Yildirim The arginine methyltransferase PRMT7 promotes extravasation of monocytes resulting in tissue injury in COPD Nature Communications, 2022-03-14;13(1):1303. 3/14/2022 [PMID: 35288557]
• Jiang L, Yilmaz M, Uehara M et al. Characterization of Leptin Receptor+ Stromal Cells in Lymph Node Frontiers in Immunology 1/17/2022 [PMID: 35111151]
• TY Kim, S Kim, Y Kim, YS Lee, S Lee, SH Lee, MN Kweon A High-Fat Diet Activates the BAs-FXR Axis and Triggers Cancer-Associated Fibroblast Properties in the Colon Cellular and Molecular Gastroenterology and Hepatology, 2021-12-29;0(0):. 2021-12-29 [PMID: 34971821] (IHC, Mouse)
• G Baranwal, HA Creed, LM Black, A Auger, AM Quach, R Vegiraju, HE Eckenrode, A Agarwal, JM Rutkowski Expanded renal lymphatics improve recovery following kidney injury Physiological Reports, 2021-11-01;9(22):e15094. 2021-11-01 [PMID: 34806312] (IHC, Mouse)
• X Liu, M Bai, Y Sun, X Hu, C Wang, J Xie, L Ye FGF7-induced E11 facilitates cell-cell communication through connexin43 International journal of biological sciences, 2021-09-03;17(14):3862-3874. 9/3/2021 [PMID: 34671204]
• L Johnson Ch, H Lahlou, C Steinacher, S Assou, Y Messat, J Dudás, A Edge, B Crespo, M Crosier, C Sergi, A Schrott-Fi, A Zine Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs International Journal of Molecular Sciences, 2021-10-07;22(19):. 2021-10-07 [PMID: 34639189] (IF, Mouse)
• D Chanda, M Rehan, SR Smith, KG Dsouza, Y Wang, K Bernard, D Kurundkar, V Memula, K Kojima, JA Mobley, GA Benavides, V Darley-Usm, YI Kim, JW Zmijewski, JS Deshane, S De Langhe, VJ Thannickal Mesenchymal stromal cell aging impairs the self-organizing capacity of lung alveolar epithelial stem cells Elife, 2021-09-16;10(0):. 2021-09-16 [PMID: 34528872] (IHC, Mouse)
• Ophir Shani, Yael Raz, Lea Monteran, Ye'ela Scharff, Oshrat Levi-Galibov, Or Megides et al. Evolution of fibroblasts in the lung metastatic microenvironment is driven by stage-specific transcriptional plasticity eLife 6/25/2021 [PMID: 34169837]
• D Sz?ke, G Kovács, É Kemecsei, L Bálint, K Szoták-Ajt, P Aradi, A Styevkóné, BL Mui, YK Tam, TD Madden, K Karikó, RP Kataru, MJ Hope, D Weissman, BJ Mehrara, N Pardi, Z Jakus Nucleoside-modified VEGFC mRNA induces organ-specific lymphatic growth and reverses experimental lymphedema Nature Communications, 2021-06-08;12(1):3460. 2021-06-08 [PMID: 34103491] (IHC, Mouse)
• Wanshu Ma, Hyea Jin Gil, Xiaolei Liu, Lauren P. Diebold, Marc A. Morgan, Michael J. Oxendine-Burns et al. Mitochondrial respiration controls the Prox1-Vegfr3 feedback loop during lymphatic endothelial cell fate specification and maintenance Science Advances 4/30/2021 [PMID: 33931446]
• R Luo, Y Cheng, D Chang, T Liu, L Liu, G Pei, N Zhang, Z Wang, K Guo, W Chen, M Li, L Fan, C Zhang, Y Li, W Dai, M Zuo, Y Xu, Y Yao, S Ge, G Xu Tertiary lymphoid organs are associated with the progression of kidney damage and regulated by interleukin-17A Theranostics, 2021-01-01;11(1):117-131. 2021-01-01 [PMID: 33391465] (IHC, Human)
• LouJin Song, Xian Chen, Terri A Swanson, Brianna LaViolette, Jincheng Pang, Teresa Cunio et al. Lymphangiogenic therapy prevents cardiac dysfunction by ameliorating inflammation and hypertension eLife 11/17/2020 [PMID: 33200983]
• J Weber, S Rajan, C Schremmer, YK Chao, G Krasteva-C, M Kannler, AÖ Yildirim, M Brosien, J Schredelse, N Weissmann, C Grimm, T Gudermann, A Dietrich TRPV4 channels are essential for alveolar epithelial barrier function as protection from lung edema JCI Insight, 2020-10-15;0(0):. 2020-10-15 [PMID: 32931478] (Western Blot, Mouse)
• M Strunz, LM Simon, M Ansari, JJ Kathiriya, I Angelidis, CH Mayr, G Tsidiridis, M Lange, LF Mattner, M Yee, P Ogar, A Sengupta, I Kukhtevich, R Schneider, Z Zhao, C Voss, T Stoeger, JHL Neumann, A Hilgendorf, J Behr, M O'Reilly, M Lehmann, G Burgstalle, M Königshoff, HA Chapman, FJ Theis, HB Schiller Alveolar regeneration through a Krt8+ transitional stem cell state that persists in human lung fibrosis Nat Commun, 2020-07-16;11(1):3559. 2020-07-16 [PMID: 32678092] (IHC, Western Blot, Mouse)
• M Sesarti?, K Ikenberg, SY Yoon, M Detmar Keratinocyte-Expressed Podoplanin is Dispensable for Multi-Step Skin Carcinogenesis Cells, 2020-06-24;9(6):. 2020-06-24 [PMID: 32599908] (IHC, Transgenic Mouse)
• X Li, J Zhao, V Kasinath, M Uehara, L Jiang, N Banouni, MM McGrath, T Ichimura, P Fiorina, DR Lemos, SR Shin, CF Ware, JS Bromberg, R Abdi Lymph node fibroblastic reticular cells deposit fibrosis-associated collagen following organ transplantation J. Clin. Invest., 2020-08-03;0(0):. 2020-08-03 [PMID: 32597832] (IF, Mouse)
• A Chakrabort, CK Scogin, K Rizwan, TS Morley, JM Rutkowski Characterizing Lymphangiogenesis and Concurrent Inflammation in Adipose Tissue in Response to VEGF-D Front Physiol, 2020-04-22;11(0):363. 2020-04-22 [PMID: 32390866] (IHC-P, Mouse)
• Kitti Szoták-Ajtay, Dániel Szõke, Gábor Kovács, Judit Andréka, Gábor B. Brenner, Zoltán Giricz et al. Reduced Prenatal Pulmonary Lymphatic Function Is Observed in Clp1 K/K Embryos With Impaired Motor Functions Including Fetal Breathing Movements in Preparation of the Developing Lung for Inflation at Birth Frontiers in Bioengineering and Biotechnology 3/6/2020 [PMID: 32211389]
• Kitti Szoták-Ajtay, Dániel Szõke, Gábor Kovács, Judit Andréka, Gábor B. Brenner, Zoltán Giricz et al. Reduced Prenatal Pulmonary Lymphatic Function Is Observed in Clp1 K/K Embryos With Impaired Motor Functions Including Fetal Breathing Movements in Preparation of the Developing Lung for Inflation at Birth Frontiers in Bioengineering and Biotechnology 2020-03-06 [PMID: 32211389] (Immunohistochemistry, Transgenic Mouse)
• Kuei-Pin Chung, Chia-Lang Hsu, Li-Chao Fan, Ziling Huang, Divya Bhatia, Yi-Jung Chen et al. Mitofusins regulate lipid metabolism to mediate the development of lung fibrosis Nature Communications 7/29/2019 [PMID: 31358769]
• KA Staines, E Ikpegbu, AE Törnqvist, S Dillon, B Javaheri, AK Amin, DN Clements, DJ Buttle, AA Pitsillide, C Farquharso Conditional deletion of E11/podoplanin in bone protects against load-induced osteoarthritis BMC Musculoskelet Disord, 2019-07-27;20(1):344. 2019-07-27 [PMID: 31351471] (IHC-P, Canine, Human, Mouse)
• SY Yoon, LC Dieterich, S Karaman, ST Proulx, SB Bachmann, C Sciaroni, M Detmar An important role of cutaneous lymphatic vessels in coordinating and promoting anagen hair follicle growth PLoS ONE, 2019-07-25;14(7):e0220341. 2019-07-25 [PMID: 31344105] (IHC, Mouse)
• Hélène Lazareth, Carole Henique, Olivia Lenoir, Victor G. Puelles, Martin Flamant, Guillaume Bollée et al. The tetraspanin CD9 controls migration and proliferation of parietal epithelial cells and glomerular disease progression Nature Communications 7/24/2019 [PMID: 31341160]
• SY Yoon, LC Dieterich, C Tacconi, M Sesartic, Y He, L Brunner, O Kwon, M Detmar An important role of podoplanin in hair follicle growth PLoS ONE, 2019-07-23;14(7):e0219938. 2019-07-23 [PMID: 31335913] (IHC-Fr, Mouse)
• P Chen, S Wang, KS Janardhan, RL Zemans, W Deng, P Karmaus, S Shen, M Sunday, LG Que, MB Fessler, XP Zhong Efficient CD4Cre-Mediated Conditional KRas Expression in Alveolar Macrophages and Alveolar Epithelial Cells Causes Fatal Hyperproliferative Pneumonitis J. Immunol., 2019-07-17;0(0):. 2019-07-17 [PMID: 31315887] (IHC-P, Mouse)
• H Tanaka, K Horioka, M Yamamoto, M Asari, K Okuda, K Yamazaki, K Shimizu, K Ogawa Overproduction of thrombopoietin by BRAFV600E-mutated mouse hepatocytes and contribution of thrombopoietin to hepatocarcinogenesis Cancer Sci., 2019-07-30;110(9):2748-2759. 2019-07-30 [PMID: 31301081] (IHC-P, Mouse)
• Maria Cimini, Venkata Naga Srikanth Garikipati, Claudio de Lucia, Zhongjian Cheng, Chunlin Wang, May M. Truongcao et al. Podoplanin neutralization improves cardiac remodeling and function after acute myocardial infarction JCI Insight 8/8/2019 [PMID: 31287805]
• X Li, A Bean, M Uehara, N Banouni, M Ben Nasr, V Kasinath, L Jiang, P Fiorina, R Abdi Immune heterogeneity of head and tail pancreatic lymph nodes in non-obese diabetic mice Sci Rep, 2019-07-05;9(1):9778. 2019-07-05 [PMID: 31278331] (IHC-Fr, Mouse)
• M Uehara, X Li, A Sheikhi, N Zandi, B Walker, B Saleh, N Banouni, L Jiang, F Ordikhani, L Dai, M Yonar, I Vohra, V Kasinath, DP Orgill, A Khademhoss, N Annabi, R Abdi Anti-IL-6 eluting immunomodulatory biomaterials prolong skin allograft survival Sci Rep, 2019-04-25;9(1):6535. 2019-04-25 [PMID: 31024011] (IHC-Fr, Mouse)
• Maria A.S. Broggi, Lea Maillat, Cristina C. Clement, Natacha Bordry, Patricia Corthésy, Aymeric Auger et al. Tumor-associated factors are enriched in lymphatic exudate compared to plasma in metastatic melanoma patients Journal of Experimental Medicine 5/6/2019 [PMID: 30975896]
• Kun Wang, Lisa Le, Brad M. Chun, LeAnn M. Tiede‐Lewis, Lora A. Shiflett, Matthew Prideaux et al. A Novel Osteogenic Cell Line That Differentiates Into GFP‐Tagged Osteocytes and Forms Mineral With a Bone‐Like Lacunocanalicular Structure Journal of Bone and Mineral Research 6/7/2019 [PMID: 30882939]
• Kun Wang, Lisa Le, Brad M. Chun, LeAnn M. Tiede‐Lewis, Lora A. Shiflett, Matthew Prideaux et al. A Novel Osteogenic Cell Line That Differentiates Into GFP‐Tagged Osteocytes and Forms Mineral With a Bone‐Like Lacunocanalicular Structure Journal of Bone and Mineral Research 2019-06-07 [PMID: 30882939] (Immunohistochemistry, Western Blot, Mouse)
• Vivek Kasinath, Osman Arif Yilmam, Mayuko Uehara, Merve Yonar, Liwei Jiang, Xiaofei Li et al. Urine podoplanin heralds the onset of ischemia-reperfusion injury of the kidney American Journal of Physiology-Renal Physiology 5/1/2019 [PMID: 30864839]
• Catherine L. Ly, Gabriela D. García García Nores, Raghu P. Kataru, Babak J. Mehrara T helper 2 differentiation is necessary for development of lymphedema Translational Research 4/1/2019 [PMID: 30633890]
• Ryan S. Lane, Julia Femel, Alec P. Breazeale, Christopher P. Loo, Guillaume Thibault, Andy Kaempf et al. IFN gamma -activated dermal lymphatic vessels inhibit cytotoxic T cells in melanoma and inflamed skin Journal of Experimental Medicine 12/3/2018 [PMID: 30381467]
• L Liu, L Zhao, Y Yang, J Gao, C Hu, B Guo, B Zhu Cytotoxic chemotherapy reduces T cell trafficking to the spleen by downregulating the expression of C-C motif chemokine ligand 21 and C-C motif chemokine ligand 19 Oncol Lett, 2018-08-09;16(4):5013-5019. 2018-08-09 [PMID: 30250567]
• Q Ma, LC Dieterich, K Ikenberg, SB Bachmann, J Mangana, ST Proulx, VC Amann, MP Levesque, R Dummer, P Baluk, DM McDonald, M Detmar Unexpected contribution of lymphatic vessels to promotion of distant metastatic tumor spread Sci Adv, 2018-08-08;4(8):eaat4758. 2018-08-08 [PMID: 30101193] (IHC, Mouse)
• OH Maarouf, M Uehara, V Kasinath, Z Solhjou, N Banouni, B Bahmani, L Jiang, OA Yilmam, I Guleria, SB Lovitch, JL Grogan, P Fiorina, PT Sage, JS Bromberg, MM McGrath, R Abdi Repetitive ischemic injuries to the kidneys result in lymph node fibrosis and impaired healing JCI Insight, 2018-07-12;3(13):. 2018-07-12 [PMID: 29997302] (Flow Cytometry, Mouse)
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  Details:
  Citation used the HRP format of this antibody.
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Review for Podoplanin Antibody (AF3244) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer ICC Mouse 01/07/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: See PMID 23892627
• Species: Mouse

Bioinformatics

• Gene Symbol: PDPN
• Uniprot:
  • Q546R8()