Podoplanin Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) followed by HRP-conjugated ...read more
Podoplanin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) at 15 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Podoplanin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Podoplanin
Gln21-Lys133
Accession # Q546R8
Specificity
Detects mouse Podoplanin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Podoplanin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDPN
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF3244 in the following applications:

Publications
Read Publications using
AF3244 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Podoplanin Antibody [Unconjugated]

  • 36-KD
  • Aggrus
  • Gp36
  • Gp38
  • GP40
  • HT1A-1
  • hT1alpha-1
  • hT1alpha-2
  • lung type I cell membrane associated glycoprotein
  • lung type-I cell membrane-associated glycoprotein (T1A-2)
  • OTS 8
  • OTS8
  • PA2.26 antigen
  • PDPN
  • Podoplanin
  • RANDAM-2
  • T1A
  • T1A-2
  • T1-alpha

Background

Podoplanin, also known as T1 alpha (T1 alpha ), is a mucin type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. In addition, non-endothelial cells in numerous normal tissues also express the protein. Within the region used as the immunogen, mouse Podoplanin shares 73.5% and 29% amino acid sequence homology with rat and human Podoplanin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-92630
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF743
Species: Mu
Applications: WB, Flow, CyTOF-ready
AF3628
Species: Mu, Rt
Applications: WB, Flow, IHC, CyTOF-ready, ICC
NB110-41083
Species: Hu
Applications: WB, ChIP, ELISA, ICC/IF
NB600-1071
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-89284
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P, KD
H00005324-M02
Species: Hu, Mu
Applications: WB, ChIP, ELISA, IHC, IHC-P
NBP1-32920
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, KD
NBP1-87217
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IF
NBP2-13669
Species: Hu
Applications: ICC/IF, IHC, IHC-P

Publications for Podoplanin Antibody (AF3244)(35)

We have publications tested in 2 confirmed species: Mouse, Canine.

We have publications tested in 8 applications: Flow Cytometry, ICC, IF, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(2)
IF
(1)
IHC
(7)
IHC-Fr
(10)
IHC-P
(8)
Neutralization
(1)
Western Blot
(4)
All Applications
Filter By Species
Mouse
(33)
Canine
(1)
All Species
Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using AF3244 Applications Species
J Weber, S Rajan, C Schremmer, YK Chao, G Krasteva-C, M Kannler, AÖ Yildirim, M Brosien, J Schredelse, N Weissmann, C Grimm, T Gudermann, A Dietrich TRPV4 channels are essential for alveolar epithelial barrier function as protection from lung edema JCI Insight, 2020;0(0):. 2020 [PMID: 32931478] (Western Blot, Mouse) Western Blot Mouse
M Strunz, LM Simon, M Ansari, JJ Kathiriya, I Angelidis, CH Mayr, G Tsidiridis, M Lange, LF Mattner, M Yee, P Ogar, A Sengupta, I Kukhtevich, R Schneider, Z Zhao, C Voss, T Stoeger, JHL Neumann, A Hilgendorf, J Behr, M O'Reilly, M Lehmann, G Burgstalle, M Königshoff, HA Chapman, FJ Theis, HB Schiller Alveolar regeneration through a Krt8+ transitional stem cell state that persists in human lung fibrosis Nat Commun, 2020;11(1):3559. 2020 [PMID: 32678092] (Western Blot, Mouse) Western Blot Mouse
X Li, J Zhao, V Kasinath, M Uehara, L Jiang, N Banouni, MM McGrath, T Ichimura, P Fiorina, DR Lemos, SR Shin, CF Ware, JS Bromberg, R Abdi Lymph node fibroblastic reticular cells deposit fibrosis-associated collagen following organ transplantation J. Clin. Invest., 2020;0(0):. 2020 [PMID: 32597832] (IF, Mouse) IF Mouse
A Chakrabort, CK Scogin, K Rizwan, TS Morley, JM Rutkowski Characterizing Lymphangiogenesis and Concurrent Inflammation in Adipose Tissue in Response to VEGF-D Front Physiol, 2020;11(0):363. 2020 [PMID: 32390866] (IHC-P, Mouse) IHC-P Mouse
KA Staines, E Ikpegbu, AE Törnqvist, S Dillon, B Javaheri, AK Amin, DN Clements, DJ Buttle, AA Pitsillide, C Farquharso Conditional deletion of E11/podoplanin in bone protects against load-induced osteoarthritis BMC Musculoskelet Disord, 2019;20(1):344. 2019 [PMID: 31351471] (IHC-P, Mouse) IHC-P Mouse
KA Staines, E Ikpegbu, AE Törnqvist, S Dillon, B Javaheri, AK Amin, DN Clements, DJ Buttle, AA Pitsillide, C Farquharso Conditional deletion of E11/podoplanin in bone protects against load-induced osteoarthritis BMC Musculoskelet Disord, 2019;20(1):344. 2019 [PMID: 31351471] (IHC-P, Canine) IHC-P Canine
SY Yoon, LC Dieterich, S Karaman, ST Proulx, SB Bachmann, C Sciaroni, M Detmar An important role of cutaneous lymphatic vessels in coordinating and promoting anagen hair follicle growth PLoS ONE, 2019;14(7):e0220341. 2019 [PMID: 31344105] (IHC, Mouse) IHC Mouse
SY Yoon, LC Dieterich, C Tacconi, M Sesartic, Y He, L Brunner, O Kwon, M Detmar An important role of podoplanin in hair follicle growth PLoS ONE, 2019;14(7):e0219938. 2019 [PMID: 31335913] (IHC-Fr, Mouse) IHC-Fr Mouse
P Chen, S Wang, KS Janardhan, RL Zemans, W Deng, P Karmaus, S Shen, M Sunday, LG Que, MB Fessler, XP Zhong Efficient CD4Cre-Mediated Conditional KRas Expression in Alveolar Macrophages and Alveolar Epithelial Cells Causes Fatal Hyperproliferative Pneumonitis J. Immunol., 2019;0(0):. 2019 [PMID: 31315887] (IHC-P, Mouse) IHC-P Mouse
H Tanaka, K Horioka, M Yamamoto, M Asari, K Okuda, K Yamazaki, K Shimizu, K Ogawa Overproduction of thrombopoietin by BRAFV600E-mutated mouse hepatocytes and contribution of thrombopoietin to hepatocarcinogenesis Cancer Sci., 2019;110(9):2748-2759. 2019 [PMID: 31301081] (IHC-P, Mouse) IHC-P Mouse
Show All 35 Publications.

Review for Podoplanin Antibody (AF3244) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF3244:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
ICC Mouse 01/07/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 23892627
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23892627

Details

Detection NotesSee PMID 23892627

Comments

CommentsPublished: See PMID 23892627Independent review from antibodyresource.com

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Podoplanin Antibody (AF3244) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF3244

Additional Podoplanin Products

Bioinformatics Tool for Podoplanin Antibody (AF3244)

Discover related pathways, diseases and genes to Podoplanin Antibody (AF3244). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Podoplanin Antibody (AF3244)

Discover more about diseases related to Podoplanin Antibody (AF3244).
 

Pathways for Podoplanin Antibody (AF3244)

View related products by pathway.

PTMs for Podoplanin Antibody (AF3244)

Learn more about PTMs related to Podoplanin Antibody (AF3244).

Blogs on Podoplanin.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein)
Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Verified Customer
01/07/2015
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol PDPN
Uniprot