AF9 Antibody

Images

 
Western Blot: AF9 Antibody [NBP2-15303] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: AF9 Antibody [NBP2-15303] - AF9 antibody detects AF9 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. ...read more
Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303] - Mouse liver. AF9 stained by AF9 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: AF9 Antibody [NBP2-15303] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody diluted at 1:500. ...read more
Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303] - D54 xenograft, using AF9 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303] - Mouse brain. AF9 stained by AF9 antibody diluted at 1:1500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

AF9 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human AF9. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MLLT3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation Sequencing -Assay dependent
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP2-15303.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS pH7, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for AF9 Antibody

  • AF-9
  • AF9myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 3
  • ALL1-fused gene from chromosome 9 protein
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocatedto, 3
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 3
  • Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein
  • protein AF-9
  • YEATS domain-containing protein 3
  • YEATS3FLJ2035

Background

The human AF9 gene is one of the most common fusion partner genes with the ALL1 gene at 11q23 (also called MLL), resulting in the t(9;11)(p22;q23) fusion. The AF9 gene is more than 100 kb, and 2 patient breakpoint cluster regions (BCRs) have been identified. BCR1 is found within intron 4, previously called site A, whereas BCR2 or site B spans introns 7 and 8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AF9 Antibody (NBP2-15303)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol MLLT3
Uniprot