p27/Kip1 [p Thr198] Antibody [Unconjugated]

Western blot shows lysates of MCF-7 human breast cancer cell line, non-transfected, transfected with plasmid expressing YFP-p27/Kip1 wild type (WT), or transfected with YFP-p27/Kip1 T198A mutant (T198A). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-p27/Kip1 (T198) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3994) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p27/Kip1 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

p27/Kip1 [p Thr198] Antibody [Unconjugated] Summary

• Immunogen: Phosphopeptide containing human p27/Kip1 T198 site
• Modification: p Thr198
• Specificity: Detects human p27/Kip1 when phosphorylated at T198. Does not recognize p27/Kip1 when unphosphorylated at T198.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDKN1B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p27/Kip1 [p Thr198] Antibody [Unconjugated]

• CDKN1B
• CDKN4
• cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• cyclin-dependent kinase inhibitor 1B
• Cyclin-dependent kinase inhibitor p27
• Kip1
• KIP1P27KIP1
• MEN1B
• MEN4
• p27
• p27Kip1

Background

The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle. p27/Kip1 is phosphorylated at T198 in cells treated with the AMP-dependent protein kinase (AMPK) activator AICAR (5-amino-4‑imidazolecarboxamide riboside) or glucose withdrawal.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p27/Kip1 Antibody (AF3994)(20)

Publications using AF3994

• A Nowosad, P Jeannot, C Callot, J Creff, RT Perchey, C Joffre, P Codogno, S Manenti, A Besson p27 controls Ragulator and mTOR activity in amino acid-deprived cells to regulate the autophagy-lysosomal pathway and coordinate cell cycle and cell growth Nat. Cell Biol., 2020-08-17;22(9):1076-1090. 2020-08-17 [PMID: 32807902] (Western Blot, Human)
• YF Lian, YL Huang, YJ Zhang, DM Chen, JL Wang, H Wei, YH Bi, ZW Jiang, P Li, MS Chen, YH Huang CACYBP Enhances Cytoplasmic Retention of P27Kip1 to Promote Hepatocellular Carcinoma Progression in the Absence of RNF41 Mediated Degradation Theranostics, 2019-10-22;9(26):8392-8408. 2019-10-22 [PMID: 31754404] (Western Blot, Human)
• CF Theriau, MK Connor Voluntary physical activity counteracts the proliferative tumor growth microenvironment created by adipose tissue via high-fat diet feeding in female rats Physiol Rep, 2017-07-01;5(13):. 2017-07-01 [PMID: 28676553] (Western Blot, Human)
• Dai Horiuchi, Roman Camarda, Alicia Y. Zhou, Christina Yau, Olga Momcilovic, Sanjeev Balakrishnan et al. PIM kinase inhibition presents a novel targeted therapy against triple-negative breast tumors with elevated MYC expression Nature Medicine 11/1/2016 [PMID: 27775705]
• Dai Horiuchi, Roman Camarda, Alicia Y. Zhou, Christina Yau, Olga Momcilovic, Sanjeev Balakrishnan et al. PIM kinase inhibition presents a novel targeted therapy against triple-negative breast tumors with elevated MYC expression Nature Medicine 2016-11-01 [PMID: 27775705] (Western Blot, Human, Mouse)
• Na Zhou, Shoudao Yuan, Rongchun Wang, Weifang Zhang, Jason J. Chen Role of dual specificity tyrosine-phosphorylation-regulated kinase 1B (Dyrk1B) in S-phase entry of HPV E7 expressing cells from quiescence Oncotarget 8/19/2015 [PMID: 26307683]
• Hogel H, Miikkulainen P, Bino L, Jaakkola PM. Hypoxia inducible prolyl hydroxylase PHD3 maintains carcinoma cell growth by decreasing the stability of p27. Mol. Cancer. 7/30/2015 [PMID: 26223520]
  
  Details:
  PHD1 antibody used for WB on 786-O cell under hypoxia (1% O2) and normoxia (21 % O2) by siPHD1 (Figure 1C).
• Carmela De Marco, Donatella Malanga, Nicola Rinaldo, Fernanda De Vita, Marianna Scrima, Sara Lovisa et al. Mutant AKT1-E17K is oncogenic in lung epithelial cells Oncotarget 5/25/2015 [PMID: 26053093]
• Zhao D, Besser A, Wander S, Sun J, Zhou W, Wang B, Ince T, Durante M, Guo W, Mills G, Theodorescu D, Slingerland J Cytoplasmic p27 promotes epithelial-mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation. Oncogene, 2015-02-16;34(43):5447-59. 2015-02-16 [PMID: 25684140] (Immunoprecipitation, Western Blot, Human)
• Yanagi T, Krajewska M, Matsuzawa SI et al. PCTAIRE1 phosphorylates p27 and regulates mitosis in cancer cells. Cancer Res 10/15/2014 [PMID: 25205104]
• Moros A, Bustany S, Cahu J, Saborit-Villarroya I, Martinez A, Colomer D, Sola B, Roue G Antitumoral activity of lenalidomide in in vitro and in vivo models of mantle cell lymphoma involves the destabilization of cyclin D1/p27KIP1 complexes. Clin Cancer Res, 2013-10-31;20(2):393-403. 2013-10-31 [PMID: 24178620] (ICC, Immunoprecipitation, Western Blot, Human)
• Lin , Victor T, Lin , Vivian Y, Lai , Yun-Ju, Chen , Chen-Sha, Liu , Kang, Lin , Weei-Chi, Lin , Fang-Tsy TRIP6 regulates p27 KIP1 to promote tumorigenesis. Mol Cell Biol, 2013-01-22;33(7):1394-409. 2013-01-22 [PMID: 23339869] (Western Blot, Human)
• Dumitru CA, Fechner MK, Hoffmann TK A novel p38-MAPK signaling axis modulates neutrophil biology in head and neck cancer. J. Leukoc. Biol., 2012-01-18;91(4):591-8. 2012-01-18 [PMID: 22262799] (Western Blot, Human)
• Uranbileg B, Yamamoto H, Park JH Cdc6 protein activates p27KIP1-bound Cdk2 protein only after the bound p27 protein undergoes C-terminal phosphorylation. J. Biol. Chem., 2012-01-05;287(9):6275-83. 2012-01-05 [PMID: 22223646] (Western Blot, Rat)
• Wen S, So Y, Singh K Promotion of cytoplasmic mislocalization of p27 by Helicobacter pylori in gastric cancer. Oncogene, 2011-08-15;31(14):1771-80. 2011-08-15 [PMID: 21841827] (Western Blot, Human)
• Pierantoni GM, Esposito F, Tornincasa M, Rinaldo C, Viglietto G, Soddu S, Fusco A Homeodomain-interacting Protein Kinase-2 Stabilizes p27kip1 by Its Phosphorylation at Serine 10 and Contributes to Cell Motility. J. Biol. Chem., 2011-06-29;286(33):29005-13. 2011-06-29 [PMID: 21715331] (Western Blot, Human)
• Donohoe DR, Garge N, Zhang X, Sun W, O'Connell TM, Bunger MK, Bultman SJ The microbiome and butyrate regulate energy metabolism and autophagy in the mammalian colon. Cell Metab., 2011-05-04;13(5):517-26. 2011-05-04 [PMID: 21531334] (Western Blot, Mouse)
• Santi SA, Lee H Ablation of Akt2 induces autophagy through cell cycle arrest, the downregulation of p70S6K, and the deregulation of mitochondria in MDA-MB231 cells. PLoS ONE, 2011-01-31;6(1):e14614. 2011-01-31 [PMID: 21297943] (Western Blot, Human)
• Bustany S, Tchakarska G, Sola B Cyclin D1 regulates p27(Kip1) stability in B cells. Cell. Signal., 2010-09-15;23(1):171-9. 2010-09-15 [PMID: 20837141] (Western Blot, Mouse)
• Kim J, Jonasch E, Alexander A, Short JD, Cai S, Wen S, Tsavachidou D, Tamboli P, Czerniak BA, Do KA, Wu KJ, Marlow LA, Wood CG, Copland JA, Walker CL Cytoplasmic sequestration of p27 via AKT phosphorylation in renal cell carcinoma. Clin. Cancer Res., 2009-01-01;15(1):81-90. 2009-01-01 [PMID: 19118035] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CDKN1B