LXR Antibody

Images

 
Western Blot: LXR Antibody [NB400-157] - WB detection of LXR in human liver.
Simple Western: LXR Antibody [NB400-157] - Simple Western lane view shows a ~49 kDa for LXR in human skeletal muscle tissue lysate under reducing conditions. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Po, Pm, Rt, ChSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.04 mg/ml

LXR Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human LXR protein sequence (between residues 50-150). [UniProt# Q13133]
Localization
Nuclear (potential)
Predicted Species
Rat (92%), Chicken (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR1H3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Simple Western
Application Notes
This LXR antibody is useful for Western blot, where a band is observed at ~50 kDa.
Positive Control
Liver Lysate (NB820-59232)
Control Peptide
LXR Peptide (NB400-157PEP)
Reviewed Applications
Read 1 Review rated 3
using
NB400-157 in the following applications:

Publications
Read Publications using
NB400-157 in the following applications:

  • WB
    7 publications

Reactivity Notes

Human, mouse, porcine and monkey. Immunogen sequence has 92% identity to rat and 90% identity to chicken.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.04 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LXR Antibody

  • alpha
  • Liver X receptor alpha
  • liver X receptor-alpha
  • LXRA
  • Nuclear receptor subfamily 1 group H member 3
  • nuclear receptor subfamily 1, group H, member 3
  • oxysterols receptor LXR-alpha
  • RLD-1

Background

LXR (liver X receptors - LXR-alpha and LXR-beta, encoded by NR1H3 and NR1H2, respectively) belongs to nuclear hormone receptor superfamily and are ligand-dependent nuclear transcription factors which are activated by oxysterols. LXR beta is ubiquitously expressed whereas LXR alpha is expressed mainly in liver, intestine, adipose tissue and macrophages. LXR-alpha and LXR-beta shares 78% similarity within their ligand-binding domains and form obligate heterodimers with retinoic X receptor (RXR), bind specific response elements within promoters of target genes ABCA1, ABCG1, SREBF1, APOE etc., and regulate their expression. In the unliganded state, LXRs binds with corepressors such as N-CoR which displaces upon ligand binding. Oxysterols (oxidized cholesterol derivatives) are their natural ligands whereas endogenous ligands includes 22(R)-hydroxycholesterol (22-HC), 24(S)-hydroxycholesterol (24(S)-HC), 24,25-EC, 27-hydroxycholesterol etc. LXRs control the major metabolic pathways such as cholesterol homeostasis, lipogenesis, inflammation, toxicity proliferation cell survival/death and different aspacts of acquired/innate immunity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LXR Antibody (NB400-157)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 1 application: WB.


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WB
(7)
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Human
(5)
Mouse
(2)
Porcine
(2)
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Showing Publications 1 - 9 of 9.
Publications using NB400-157 Applications Species
Mohammadi Abbas, Oshaghi Ebrahim Abbasi. Effect of garlic on lipid profile and expression of LXR alpha in intestine and liver of hypercholesterolemic mice. J Diabetes Metab Disord. 2014 Jan 29 [PMID: 24476027] (WB, Mouse) WB Mouse
Yoon HS, Ju JH, Kim HN et al. Reduction in cholesterol absorption in Caco-2 cells through the down-regulation of Niemann-Pick C1-like 1 by the putative probiotic strains Lactobacillus rhamnosus BFE5264 and Lactobacillus plantarum NR74 from fermented foods Int J Food Sci Nutr 2012 Jul 20 [PMID: 22816655] (WB, Human) WB Human
Yoon HS, Ju JH, Lee JE et al. The probiotic Lactobacillus rhamnosus BFE5264 and Lactobacillus plantarum NR74 promote cholesterol efflux and suppress inflammation in THP-1 cells J Sci Food Agric 2013 Mar 15 [PMID: 22806829] (WB, Human) WB Human
Aye IL, Waddell BJ, Mark PJ, Keelan JA. Oxysterols inhibit differentiation and fusion of term primary trophoblasts by activating liver X receptors. Placenta 2011 Feb [PMID: 21208656] (Human) Human
Dang H et al. Suppression of 2,3-oxidosqualene cyclase by high fat diet contributes to liver X receptor-alpha-mediated improvement of hepatic lipid profile. J Biol Chem;284(10):6218-26. 2009 Mar 6. [PMID: 19119143] (WB, Human, Mouse) WB Human, Mouse
Qiu G, Hill JS Atorvastatin inhibits ABCA1 expression and cholesterol efflux in THP-1 macrophages by an LXR-dependent pathway. J Cardiovasc Pharmacol 2008 Apr [PMID: 18427282]
Qiu, G et al. Atorvastatin decreases lipoprotein lipase and endothelial lipase expression in human THP-1 macrophages. J Lipid Res;48(10):2112-22. 2007 Oct. [PMID: 17644777] (WB, Human) WB Human
Zhang, C et al. NO-1886 upregulates ATP binding cassette transporter A1 and inhibits diet-induced atherosclerosis in Chinese Bama minipigs. J Lipid Res;47(9):2055-63. 2006 Sep. [PMID: 16807312] (WB, Porcine) WB Porcine
Zhang, C et al. Effects of NO-1886 on inflammation-associated cytokines in high-fat/high-sucrose/high-cholesterol diet-fed miniature pigs. Eur J Pharmacol;540(1-3):139-46. 2006 Jul 1. [PMID: 16730700] (WB, Porcine) WB Porcine

Review for LXR Antibody (NB400-157) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB400-157:
Filter by Applications
Simple Western
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Simple Western LXR NB400-157
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reviewed by:
Zachary Patinkin
Simple Western Human 06/24/2015
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Summary

ApplicationSimple Western
Sample TestedHuman Skeletal Muscle Lysate
SpeciesHuman
LotB-1

Blocking

Blocking DetailsProtein Simple Antibody Diluent, 5 min, 25C

Primary Anitbody

Dilution Ratio1:25, 30 min, Protein Simple Antibody Diluent

Secondary Antibody

Secondary DescriptionProtein Simple Anti-Rabbit Secondary
Secondary Manufacturer Cat#042-206
Secondary Concentrationundiluted

Details

Detection NotesWES multi-image analysis

Comments

CommentsSimple Western. Additional bands at 41 and 59 kDa.

Product General Protocols

View specific protocols for LXR Antibody (NB400-157): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LXR Antibody (NB400-157). (Showing 1 - 1 of 1 FAQs).

  1. I have a question regarding the amounts of cell extract to use with this product. You show gels from liver and skeletal muscle to demonstrate quality of the antibody, but the amounts of tissue extract used on these gels is not indicated. We are just beginning to work with this antibody, and this information would be helpful.
    • For our product NB400-157, the blot image showing the human liver lysate was run with 25ug of human liver lysate loaded into the gel. For the other image with the human skeletal muscle lysate, this blot was performed on the ProteinSimple Wes platform. This system utilizes a capillary system to separate the proteins through instead of a traditional gel. This system therefore requires much less material. For this experiment, our lab used 5ul of lysate at 0.2mg/ml.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB400-157AF405
Alexa Fluor 488 Labeled NB400-157AF488
Alexa Fluor 647 Labeled NB400-157AF647
Alexa Fluor 700 Labeled NB400-157AF700
Biotin Labeled NB400-157B
DyLight 405 Labeled NB400-157V
DyLight 488 Labeled NB400-157G
DyLight 550 Labeled NB400-157R
DyLight 650 Labeled NB400-157C
HRP Labeled NB400-157H

Additional Array Products

Array NB400-157

Bioinformatics Tool for LXR Antibody (NB400-157)

Discover related pathways, diseases and genes to LXR Antibody (NB400-157). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for LXR Antibody (NB400-157)

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Pathways for LXR Antibody (NB400-157)

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PTMs for LXR Antibody (NB400-157)

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Research Areas for LXR Antibody (NB400-157)

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Blogs on LXR.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

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Recent Reviews

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Zachary Patinkin
06/24/2015
Application: Simple Western
Species: Human

Bioinformatics

Gene Symbol NR1H3