LXR alpha/NR1H3 Antibody - BSA Free

Western Blot: LXR alpha/NR1H3 Antibody [NB400-157] - WB detection of LXR in human liver.

Simple Western: LXR alpha/NR1H3 Antibody [NB400-157] - Simple Western lane view shows a ~49 kDa for LXR in human skeletal muscle tissue lysate under reducing conditions. Image from verified customer review.

• Reactivity: Hu, Mu, Po, Pm, Rt, Ch
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.04 mg/ml

LXR alpha/NR1H3 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal portion of the human LXR alpha/NR1H3 protein sequence (between residues 50-150). [UniProt# Q13133]
• Localization: Nuclear (potential)
• Predicted Species: Rat (92%), Chicken (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR1H3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Simple Western
  • Western Blot 0.5-2 ug/ml
• Application Notes: This LXR alpha/NR1H3 antibody is useful for Western blot, where a band is observed at ~50 kDa.
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  LXR Peptide (NB400-157PEP)

Reactivity Notes

Immunogen sequence has 92% identity to rat and 90% identity to chicken.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.04 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LXR alpha/NR1H3 Antibody - BSA Free

• alpha
• Liver X receptor alpha
• liver X receptor-alpha
• LXR alpha
• LXRA
• NR1H3
• Nuclear receptor subfamily 1 group H member 3
• nuclear receptor subfamily 1, group H, member 3
• oxysterols receptor LXR-alpha
• RLD-1

Background

LXR (liver X receptors - LXR-alpha and LXR-beta, encoded by NR1H3 and NR1H2, respectively) belongs to nuclear hormone receptor superfamily and are ligand-dependent nuclear transcription factors which are activated by oxysterols. LXR beta is ubiquitously expressed whereas LXR alpha is expressed mainly in liver, intestine, adipose tissue and macrophages. LXR-alpha and LXR-beta shares 78% similarity within their ligand-binding domains and form obligate heterodimers with retinoic X receptor (RXR), bind specific response elements within promoters of target genes ABCA1, ABCG1, SREBF1, APOE etc., and regulate their expression. In the unliganded state, LXRs binds with corepressors such as N-CoR which displaces upon ligand binding. Oxysterols (oxidized cholesterol derivatives) are their natural ligands whereas endogenous ligands includes 22(R)-hydroxycholesterol (22-HC), 24(S)-hydroxycholesterol (24(S)-HC), 24,25-EC , 27-hydroxycholesterol etc. LXRs control the major metabolic pathways such as cholesterol homeostasis, lipogenesis, inflammation, toxicity proliferation cell survival/death and different aspacts of acquired/innate immunity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LXR alpha/NR1H3 Antibody (NB400-157)(10)

Publications using NB400-157

• Yang R, Zhao Y, Gu Y et al. Isocitrate dehydrogenase 1 mutation enhances 24(S)-hydroxycholesterol production and alters cholesterol homeostasis in glioma Oncogene 2020-08-27 [PMID: 32855525] (WB)
• Mohammadi Abbas, Oshaghi Ebrahim Abbasi. Effect of garlic on lipid profile and expression of LXR alpha in intestine and liver of hypercholesterolemic mice. J Diabetes Metab Disord. 2014-01-29 [PMID: 24476027] (WB, Mouse)
• Yoon HS, Ju JH, Kim HN et al. Reduction in cholesterol absorption in Caco-2 cells through the down-regulation of Niemann-Pick C1-like 1 by the putative probiotic strains Lactobacillus rhamnosus BFE5264 and Lactobacillus plantarum NR74 from fermented foods Int J Food Sci Nutr 2012-07-20 [PMID: 22816655] (WB, Human)
• Yoon HS, Ju JH, Lee JE et al. The probiotic Lactobacillus rhamnosus BFE5264 and Lactobacillus plantarum NR74 promote cholesterol efflux and suppress inflammation in THP-1 cells J Sci Food Agric 2013-03-15 [PMID: 22806829] (WB, Human)
• Aye IL, Waddell BJ, Mark PJ, Keelan JA. Oxysterols inhibit differentiation and fusion of term primary trophoblasts by activating liver X receptors. Placenta 2011-02-01 [PMID: 21208656] (Human)
• Dang H, Liu Y, Pang W et al. Suppression of 2,3-Oxidosqualene Cyclase by High Fat Diet Contributes to Liver X Receptor-{alpha}-mediated Improvement of Hepatic Lipid Profile. J Biol Chem;284(10):6218-6226. 2009-01-01 [PMID: 19119143] (WB, Mouse, Human)
• Qiu G, Hill JS Atorvastatin inhibits ABCA1 expression and cholesterol efflux in THP-1 macrophages by an LXR-dependent pathway. J Cardiovasc Pharmacol 2008-04-01 [PMID: 18427282]
• Qiu, G et al. Atorvastatin decreases lipoprotein lipase and endothelial lipase expression in human THP-1 macrophages. J Lipid Res;48(10):2112-22. 2007-10-01 [PMID: 17644777] (WB, Human)
• Zhang, C et al. NO-1886 suppressing atherosclerosis in high-fat-high-sucrose/high-cholesterol fed Bama minipigs is related to upregulating ATP-binding cassette transporter A1. J Lipid Res. 2006-01-01 [PMID: 16807312] (WB, Porcine)
• Zhang, C et al. Effects of NO-1886 on inflammation-associated cytokines in high-fat/high-sucrose/high-cholesterol diet-fed miniature pigs. Eur J Pharmacol;540(1-3):139-46. 2006-07-01 [PMID: 16730700] (WB, Porcine)

Review for LXR alpha/NR1H3 Antibody (NB400-157) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Zachary Patinkin Simple Western Human 06/24/2015

Summary

• Application: Simple Western
• Sample Tested: Human Skeletal Muscle Lysate
• Species: Human
• Lot: B-1

FAQs for LXR alpha/NR1H3 Antibody (NB400-157). (Showing 1 - 1 of 1 FAQs).

1. I have a question regarding the amounts of cell extract to use with this product. You show gels from liver and skeletal muscle to demonstrate quality of the antibody, but the amounts of tissue extract used on these gels is not indicated. We are just beginning to work with this antibody, and this information would be helpful.
   • For our product NB400-157, the blot image showing the human liver lysate was run with 25ug of human liver lysate loaded into the gel. For the other image with the human skeletal muscle lysate, this blot was performed on the ProteinSimple Wes platform. This system utilizes a capillary system to separate the proteins through instead of a traditional gel. This system therefore requires much less material. For this experiment, our lab used 5ul of lysate at 0.2mg/ml.

Bioinformatics

• Gene Symbol: NR1H3