LXR alpha/NR1H3 Antibody


Western Blot: LXR alpha/NR1H3 Antibody [NB400-157] - WB detection of LXR in human liver.
Simple Western: LXR alpha/NR1H3 Antibody [NB400-157] - Simple Western lane view shows a ~49 kDa for LXR in human skeletal muscle tissue lysate under reducing conditions. Image from verified customer review.

Product Details

Reactivity Hu, Mu, Po, Pm, Rt, ChSpecies Glossary
Applications WB, Simple Western
1.04 mg/ml

LXR alpha/NR1H3 Antibody Summary

A synthetic peptide made to an internal portion of the human LXR alpha/NR1H3 protein sequence (between residues 50-150). [UniProt# Q13133]
Nuclear (potential)
Predicted Species
Rat (92%), Chicken (90%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.5-2 ug/ml
  • Simple Western
Application Notes
This LXR alpha/NR1H3 antibody is useful for Western blot, where a band is observed at ~50 kDa.
Human Liver Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
LXR Peptide (NB400-157PEP)
Reviewed Applications
Read 1 Review rated 3
NB400-157 in the following applications:

Read Publications using
NB400-157 in the following applications:

  • WB
    8 publications

Reactivity Notes

Human, mouse, porcine and monkey. Immunogen sequence has 92% identity to rat and 90% identity to chicken.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
1.04 mg/ml
Immunogen affinity purified

Alternate Names for LXR alpha/NR1H3 Antibody

  • alpha
  • Liver X receptor alpha
  • liver X receptor-alpha
  • LXR alpha
  • LXRA
  • NR1H3
  • Nuclear receptor subfamily 1 group H member 3
  • nuclear receptor subfamily 1, group H, member 3
  • oxysterols receptor LXR-alpha
  • RLD-1


LXR (liver X receptors - LXR-alpha and LXR-beta, encoded by NR1H3 and NR1H2, respectively) belongs to nuclear hormone receptor superfamily and are ligand-dependent nuclear transcription factors which are activated by oxysterols. LXR beta is ubiquitously expressed whereas LXR alpha is expressed mainly in liver, intestine, adipose tissue and macrophages. LXR-alpha and LXR-beta shares 78% similarity within their ligand-binding domains and form obligate heterodimers with retinoic X receptor (RXR), bind specific response elements within promoters of target genes ABCA1, ABCG1, SREBF1, APOE etc., and regulate their expression. In the unliganded state, LXRs binds with corepressors such as N-CoR which displaces upon ligand binding. Oxysterols (oxidized cholesterol derivatives) are their natural ligands whereas endogenous ligands includes 22(R)-hydroxycholesterol (22-HC), 24(S)-hydroxycholesterol (24(S)-HC), 24,25-EC, 27-hydroxycholesterol etc. LXRs control the major metabolic pathways such as cholesterol homeostasis, lipogenesis, inflammation, toxicity proliferation cell survival/death and different aspacts of acquired/innate immunity.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB, ICC/IF
Species: Hu, Mu
Applications: WB, ICC/IF
Species: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Eq, Ha, Md, Pm, Rb
Applications: WB, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, Dual ISH-IHC, GS, KD, KO, PCR
Species: Hu
Applications: WB, IP, DirELISA
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IP
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Ca, Ch, SyHa, Ha, Pm
Applications: WB, Simple Western, IHC-Fr, KO
Species: Hu, Mu, Rt, Po, Ch, Fe, Ha, Pl, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP, KD
Species: Hu, Rt
Applications: WB, ICC/IF
Species: Hu
Applications: WB, ICC/IF
Species: Hu, Mu, Rt, Ha, Pm, Pm, Rb
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Po, Pm, Rt, Ch
Applications: WB, Simple Western

Publications for LXR alpha/NR1H3 Antibody (NB400-157)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 1 application: WB.

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Showing Publications 1 - 10 of 10.
Publications using NB400-157 Applications Species
Yang R, Zhao Y, Gu Y et al. Isocitrate dehydrogenase 1 mutation enhances 24(S)-hydroxycholesterol production and alters cholesterol homeostasis in glioma Oncogene Aug 27 2020 [PMID: 32855525] (WB) WB
Mohammadi Abbas, Oshaghi Ebrahim Abbasi. Effect of garlic on lipid profile and expression of LXR alpha in intestine and liver of hypercholesterolemic mice. J Diabetes Metab Disord. 2014 Jan 29 [PMID: 24476027] (WB, Mouse) WB Mouse
Yoon HS, Ju JH, Kim HN et al. Reduction in cholesterol absorption in Caco-2 cells through the down-regulation of Niemann-Pick C1-like 1 by the putative probiotic strains Lactobacillus rhamnosus BFE5264 and Lactobacillus plantarum NR74 from fermented foods Int J Food Sci Nutr 2012 Jul 20 [PMID: 22816655] (WB, Human) WB Human
Yoon HS, Ju JH, Lee JE et al. The probiotic Lactobacillus rhamnosus BFE5264 and Lactobacillus plantarum NR74 promote cholesterol efflux and suppress inflammation in THP-1 cells J Sci Food Agric 2013 Mar 15 [PMID: 22806829] (WB, Human) WB Human
Aye IL, Waddell BJ, Mark PJ, Keelan JA. Oxysterols inhibit differentiation and fusion of term primary trophoblasts by activating liver X receptors. Placenta 2011 Feb [PMID: 21208656] (Human) Human
Dang H et al. Suppression of 2,3-oxidosqualene cyclase by high fat diet contributes to liver X receptor-alpha-mediated improvement of hepatic lipid profile. J Biol Chem;284(10):6218-26. 2009 Mar 6. [PMID: 19119143] (WB, Human, Mouse) WB Human, Mouse
Qiu G, Hill JS Atorvastatin inhibits ABCA1 expression and cholesterol efflux in THP-1 macrophages by an LXR-dependent pathway. J Cardiovasc Pharmacol 2008 Apr [PMID: 18427282]
Qiu, G et al. Atorvastatin decreases lipoprotein lipase and endothelial lipase expression in human THP-1 macrophages. J Lipid Res;48(10):2112-22. 2007 Oct. [PMID: 17644777] (WB, Human) WB Human
Zhang, C et al. NO-1886 upregulates ATP binding cassette transporter A1 and inhibits diet-induced atherosclerosis in Chinese Bama minipigs. J Lipid Res;47(9):2055-63. 2006 Sep. [PMID: 16807312] (WB, Porcine) WB Porcine
Zhang, C et al. Effects of NO-1886 on inflammation-associated cytokines in high-fat/high-sucrose/high-cholesterol diet-fed miniature pigs. Eur J Pharmacol;540(1-3):139-46. 2006 Jul 1. [PMID: 16730700] (WB, Porcine) WB Porcine

Review for LXR alpha/NR1H3 Antibody (NB400-157) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB400-157:
Filter by Applications
Simple Western
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Images Ratings Applications Species Date Details
Simple Western LXR alpha/NR1H3 NB400-157
reviewed by:
Zachary Patinkin
Simple Western Human 06/24/2015


ApplicationSimple Western
Sample TestedHuman Skeletal Muscle Lysate


Blocking DetailsProtein Simple Antibody Diluent, 5 min, 25C

Primary Anitbody

Dilution Ratio1:25, 30 min, Protein Simple Antibody Diluent

Secondary Antibody

Secondary DescriptionProtein Simple Anti-Rabbit Secondary
Secondary Manufacturer Cat#042-206
Secondary Concentrationundiluted


Detection NotesWES multi-image analysis


CommentsSimple Western. Additional bands at 41 and 59 kDa.

Product General Protocols

View specific protocols for LXR alpha/NR1H3 Antibody (NB400-157): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LXR alpha/NR1H3 Antibody (NB400-157). (Showing 1 - 1 of 1 FAQs).

  1. I have a question regarding the amounts of cell extract to use with this product. You show gels from liver and skeletal muscle to demonstrate quality of the antibody, but the amounts of tissue extract used on these gels is not indicated. We are just beginning to work with this antibody, and this information would be helpful.
    • For our product NB400-157, the blot image showing the human liver lysate was run with 25ug of human liver lysate loaded into the gel. For the other image with the human skeletal muscle lysate, this blot was performed on the ProteinSimple Wes platform. This system utilizes a capillary system to separate the proteins through instead of a traditional gel. This system therefore requires much less material. For this experiment, our lab used 5ul of lysate at 0.2mg/ml.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB400-157AF350
Alexa Fluor 405 NB400-157AF405
Alexa Fluor 488 NB400-157AF488
Alexa Fluor 532 NB400-157AF532
Alexa Fluor 594 NB400-157AF594
Alexa Fluor 647 NB400-157AF647
Alexa Fluor 700 NB400-157AF700
Alexa Fluor 750 NB400-157AF750
Biotin NB400-157B
DyLight 350 NB400-157UV
DyLight 405 NB400-157V
DyLight 488 NB400-157G
DyLight 550 NB400-157R
DyLight 594 NB400-157DL594
DyLight 650 NB400-157C
DyLight 680 NB400-157FR
DyLight 755 NB400-157IR
HRP NB400-157H
Janelia Fluor 549 NB400-157JF549
Janelia Fluor 646 NB400-157JF646

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Recent Reviews


Zachary Patinkin
Application: Simple Western
Species: Human


Gene Symbol NR1H3