Western blot shows lysates of human colon tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Lumican Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2846) followed by HRP-conjugated Anti-Goat ...read more
Lumican was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Lumican Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2846) at 15 µg/mL overnight at 4 °C. Tissue ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Lumican Antibody [Unconjugated]
SLRR2DLDCKeratan sulfate proteoglycan lumican
Lumican is a 40 kDa member of the family of small leucine-rich repeat proteoglycans (SLRPs) and the class II subfamily (1). Human Lumican is synthesized as a 338 amino acid (aa) precursor that contains an 18 aa signal sequence and a 320 aa mature chain. The mature chain contains a negatively charged N-terminal domain containing sulfated tyrosine and disulfide bonds, 12 leucine-rich repeats (LRRs), four potential sites of N-linked glycosylation, and a carboxyl terminal domain containing two conserved cysteines (1). Mature human Lumican is 88%, 87%, and 70% aa identical to mature mouse, rat, and chick Lumican, respectively. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins (ECM) proteins (1, 2). Lumican is expressed in a variety of tissues, including skin, artery, lung, cornea, kidney, bone, aorta, and articular cartilage (1). Lumican’s role in vivo has been found using Lumican null mice. These mice have functional deficits including corneal opacity as well as skin and tendon fragility associated with disorganized and loosely packed collagen fibers (1, 3-6). The abnormal connective tissue phenotype seen in the Lumican null mice shows the importance of the role of Lumican in collagen fibrillogenesis (1). In addition to the control of collagen fibril assembly, Lumican has been shown to play a role in the regulation of cell proliferation (7, 8), migration (8, 9), and adhesion (9). Lumican’s overexpression has been reported in carcinoid tumor, breast, colorectal, neuroendocrine, uterine cervical and pancreatic cancers (10).
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Chakravarti, S. et al. (1998) J. Cell Biol. 141:1277.
Chakravarti, S. et al. (2000) Invest. Ophthalmol. Vis. Sci. 41:3365.
Jepsen, K.J. et al. (2002) J. Biol. Chem. 277:35532.
Chakravarti, S. et al. (2003) Invest. Ophthalmol. Vis. Sci. 44:2422.
Vuillermoz, B. et al. (2004) Exp. Cell Res. 296:294.
Nikitovic, D. et al. (2008) FEBS J. 275:350.
D’Onofrio, M.F. et al. (2008) Biochem. Biophys. Res. Commun. 365:266.
Ishiwata, T. et al. (2007) Oncol. Rep. 18:537.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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