Fibromodulin/FMOD Antibody

Western blot shows lysates of human cartilage, human ovarian cancer, and rat uterine tissue. PVDF membrane was probed with 0.3 µg/mL of Sheep Anti-Human/Mouse/Rat Fibromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5945) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fibromodulin at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Fibromodulin/FMOD Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Fibromodulin/FMOD
  Asp75-Ile376
  Accession # Q06828
• Specificity: Detects human, mouse, and rat Fibromodulin/FMOD in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: FMOD
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.3 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fibromodulin/FMOD Antibody

• Collagen-binding 59 kDa protein
• fibromodulin proteoglycan
• Fibromodulin
• FM
• FMOD
• Keratan sulfate proteoglycan fibromodulin
• SLRR2E
• SLRR2EKSPG fibromodulin

Background

Fibromodulin (FMOD; also FM and collagen-binding 59 kDa protein) is a secreted, 60‑70 kDa class II member of the SLRP family of matrix proteins. It is expressed by a variety of cell types, including keratinocytes, fibroblasts and chrondrocytes, and is known to bind to type I and XII collagen, plus TGF-beta . Fibromodulin is posited to displace lumican binding to collagen, generating 3D collagen fibrils from simple lumican-induced collagen dimers. Mature human Fibromodulin is 358 amino acids (aa) in length (aa 19‑376). It contains 12 LRRs (Leu-rich repeats) (aa 98‑376), with the seventh and eleventh LRR principally responsible for collagen type I binding. Fibromodulin contains varying amounts of keratan sulfate, and undergoes significant tyrosine sulfation. There is one splice form that shows a deletion of aa 88‑101. Over aa 76‑376, human Fibromodulin shares 96% aa identity with mouse and rat Fibromodulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibromodulin/FMOD Antibody (AF5945)(1)

Publication using AF5945

• Kolb, J;Tsata, V;John, N;Kim, K;Möckel, C;Rosso, G;Kurbel, V;Parmar, A;Sharma, G;Karandasheva, K;Abuhattum, S;Lyraki, O;Beck, T;Müller, P;Schlü beta ler, R;Frischknecht, R;Wehner, A;Krombholz, N;Steigenberger, B;Beis, D;Takeoka, A;Blümcke, I;Möllmert, S;Singh, K;Guck, J;Kobow, K;Wehner, D; Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment Nature communications 2023-10-26 [PMID: 37884489] (Immunohistochemistry, Human, Mouse)

Bioinformatics

• Gene Symbol: FMOD
• Uniprot:
  • Q06828()