PRELP Antibody [Unconjugated]

Western blot shows lysates of human liver tissue and human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PRELP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6447) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PRELP at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PRELP was detected in immersion fixed human mesenchymal stem cells differentiated into chondrocytes using Sheep Anti-Human PRELP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6447) at 10 µg/mL overnight at 4 °C. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

PRELP Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human PRELP
  Gln21-Ile382
  Accession # P51888
• Specificity: Detects human PRELP in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PRELP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PRELP Antibody [Unconjugated]

• 55 kDa leucine-rich repeat protein of articular cartilage
• MST161
• MSTP161
• PRELP
• prolargin proteoglycan
• Prolargin
• proline arginine-rich end leucine-rich repeat protein
• proline/arginine-rich end leucine-rich repeat protein
• Proline-arginine-rich end leucine-rich repeat protein
• SLRR2A
• SLRR2AMGC45323

Background

PRELP (Proline aRginine-rich End Leucine-rich repeat Protein; also Prolargin) is a 55‑62 kDa secreted glycoprotein that belongs to the small leucine-rich proteoglycan (SLRP) superfamily of extracellular matrix (ECM) molecules (1‑4). Within this family, it is considered a class II member, implying that it is unlikely to form dimeric structures (3). PRELP is synthesized as a 382 amino acid (aa) precursor that contains a 20 aa signal sequence plus a 362 aa mature region (1, 5). Like other SLRPs, PRELP contains an N-terminal extension (aa 72‑107) coupled to multiple Leu-rich repeats (LRRs) (aa 95‑382) (6). Unlike other SLRPs, PRELP does not contain any proteoglycan chains, and its N‑terminal extension is highly basic in charge. The N-terminus reportedly binds to negatively-charged heparin/heparin-sulfate, chondroitin sulfate, and Gram^- bacterial cell walls, while the LRR region participates in protein-protein interactions (7‑9). Although PRELP is known to be synthesized by only a few cell types, including osteoblasts, skeletal muscle and chondrocytes, its expression is likely to be more widespread, given its presence in the basement membrane (BM) of Bowman’s capsule, epididymal epithelium and the stratified squamous epithelium of the skin (1, 10, 11). The dual binding profile of PRELP is key to its function. In cartilage, PRELP likely links chondrocyte cell membrane heparin sulfate (HS) chains to endogenous type II collagen. Within the context of the BM, PRELP likely plays an anchoring role. The BM is composed of type IV collagen and laminin, linked together by nidogen. BM Perlecan reinforces this linkage by binding to all three components. PRELP, on the edge of the BM, can bind to free perlecan HS chains (via its N-terminus), and to underlying type I collagen (via its LRRs), thus forming an anchor for the BM (11). Notably, the N-terminus appears to do more than simply provide part of a linkage mechanism. In bone, osteoblast secreted PRELP is hypothesized to undergo proteolysis by enzymes such as LysC and glutamyl endopeptidase. This will generate 40‑75 aa N‑terminal fragments that can bind to chondroitin sulfate adducts that exist on the surface of prefusion osteoclast precursors. Following binding, PRELP is internalized, complexed to annexin-II, and translocated to the nucleus, where it interacts with NF kappa Bp65 to block osteoclast maturation (8). In tissue, PRELP may also undergo proteolytic processing during inflammation to release an N‑terminal fragment containing aa 21‑42 of the precursor (7). This sequence has been shown to possess potent antimicrobial activity by creating pores in bacterial cell walls. Mature human PRELP shares 91% aa identity with mouse PRELP (10).

1. Bengtsson, E. et al. (1995) J. Biol. Chem. 270:25639.
2. Merline, R. et al. (2009) J. Cell Commun. Signal. 3:323.
3. McEwan, P.A. et al. (2006) J. Struct. Biol. 155:294.
4. Neame, P.J. et al. (1999) Cell. Mol. Life Sci. 55:1327.
5. Grover, J. et al. (1996) Genomics 38:109.
6. SwissProt # P51888.
7. Bengtsson, E. et al. (2000) J. Biol. Chem. 275:40695.
8. Rucci, N. et. al. (2009) J. Cell Biol. 187:669.
9. Malmsten, M. et al. (2006) Matrix Biol. 25:294.
10. Grover, J. & P.J. Roughley (2001) Matrix Biol. 20:555.
11. Bengtsson, E. et al. (2002) J. Biol. Chem. 277:15061.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRELP Antibody (AF6447)(2)

Publications using AF6447

• B Chiavarina, R Ronca, Y Otaka, RB Sutton, S Rezzola, T Yokobori, P Chiodelli, R Souche, D Pourquier, A Maraver, G Faa, L Khellaf, E Turtoi, T Oyama, S Gofflot, A Bellahcène, O Detry, P Delvenne, V Castronovo, M Nishiyama, A Turtoi Fibroblast-derived prolargin is a tumor suppressor in hepatocellular carcinoma Oncogene, 2022-01-14;0(0):. 2022-01-14 [PMID: 35031773] (IHC, Human, Xenograft)
• Guanghui Liu, David Ermert, Martin E. Johansson, Birendra Singh, Yu-Ching Su, Magnus Paulsson et al. PRELP Enhances Host Innate Immunity against the Respiratory Tract Pathogen Moraxella catarrhalis The Journal of Immunology 2017-03-15 [PMID: 28148731]

Bioinformatics

• Gene Symbol: PRELP
• Uniprot:
  • P51888(Human)