Mimecan/OIF Antibody

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Mimecan/OIF Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Mimecan
  Ala20-Phe298
  Accession # Q62000
• Specificity: Detects mouse Mimecan in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Mimecan is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OGN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mimecan/OIF Antibody

• DKFZp586P2421
• KSPG25
• mimecan proteoglycan
• Mimecan
• OGN
• OIF
• OIFOG
• osteoglycin (osteoinductive factor)
• osteoglycin
• Osteoinductive factor
• SLRR3A
• SLRR3Acorneal keratan sulfate proteoglycan

Background

Mimecan, also known as KSPG25 (25 kDa Keratan Sulfate Proteoglycan) and Osteoglycin, is an extracellular matrix protein belonging to the class III subfamily of the small leucine rich proteoglycan (SLRP) family. SLRPs are characterized by leucine rich repeats in the central domain, which is flanked by N- and C-terminal cysteine‑rich domains. The core proteins contain N-linked oligosaccharides as well as glycosaminoglycan side chains. Mimecan is synthesized as a preproprotein that is processed to generate proteins of different sizes including Osteoglycin (containing 93 most C‑terminal amino acid residues) and Mimecan (containing 223 most C‑terminal residues). Mouse Mimecan shares approximately 94% and 85% amino acid sequence identity with rat and human Mimecan, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mimecan/OIF Antibody (AF2949)(4)

Publications using AF2949

• Daniella dos Santos Courrol, Cristiane Castilho Fernandes da da Silva, Luan Gavião Prado, Rosa Maria Chura-Chambi, Ligia Morganti, Gisele Oliveira de Souza et al. Leptolysin, a Leptospira secreted metalloprotease of the pappalysin family with broad-spectrum activity Frontiers in Cellular and Infection Microbiology 2022-08-23 [PMID: 36081769]
• AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases Sci Rep, 2020-07-31;10(1):12912. 2020-07-31 [PMID: 32737331] (Western Blot, Mouse)
• Zhang Y, Mao X, Schwend T, Littlechild S, Conrad G Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases. Invest Ophthalmol Vis Sci, 2013-02-05;54(2):1014-25. 2013-02-05 [PMID: 23322569] (Western Blot, Mouse)
• Zhang Y, Conrad AH, Conrad GW Effects of Ultraviolet-A and Riboflavin on the Interaction of Collagen and Proteoglycans during Corneal Cross-linking. J. Biol. Chem., 2011-02-18;286(15):13011-22. 2011-02-18 [PMID: 21335557] (Western Blot, Bovine)

Bioinformatics

• Gene Symbol: OGN
• Uniprot:
  • Q62000()