Biglycan Antibody

Biglycan was detected in immersion fixed paraffin-embedded sections of human kidney array using Human Biglycan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2667) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Biglycan Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Biglycan
  Asp38-Lys368
  Accession # P21810
• Specificity: Detects human Biglycan in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BGN
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Biglycan Antibody

• BGN
• Biglycan
• Bone/cartilage proteoglycan I
• bone/cartilage proteoglycan-I
• dermatan sulphate proteoglycan I
• DSPG1
• PGI
• PG-S1
• SLRR1A
• SLRR1Abiglycan proteoglycan
• small leucine-rich protein 1A

Background

Biglycan is a small secreted proteoglycan belonging to the leucine-rich repeat (LRR) protein family. It contains a central 12 LRR repeat domain flanked by small cysteine clusters at either side. Two glycosaminoglycans (GAG) chains are attached near the amino terminus. Biglycan binds BMP-4 and Chordin to regulate BMP-4 signaling. It may also be involved in collagen fiber assembly. Human Biglycan shares 98% amino acid sequence homology with mouse Biglycan, 97% sequence homology with rat and bovine Biglycan and 96% sequence homology with canine and equine Biglycan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Biglycan Antibody (AF2667)(9)

Publications using AF2667

• A Minns, Y Qi, K Yamamoto, K Lee, J Ahnström, S Santamaria The C-terminal domains of ADAMTS1 contain exosites involved in its proteoglycanase activity The Journal of Biological Chemistry, 2023-02-21;0(0):103048. 2023-02-21 [PMID: 36813235] (Western Blot, N/A)
• S Santamaria, DR Martin, X Dong, K Yamamoto, SS Apte, J Ahnström Post-Translational Regulation And Proteolytic Activity Of The Metalloproteinase Adamts8 The Journal of Biological Chemistry, 2021-10-21;0(0):101323. 2021-10-21 [PMID: 34687701] (Western Blot, Human)
• AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases Sci Rep, 2020-07-31;10(1):12912. 2020-07-31 [PMID: 32737331] (Western Blot, Mouse)
• S Kijani, AM Vázquez, M Levin, J Borén, P Fogelstran Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis Physiol Rep, 2017-07-01;5(14):. 2017-07-01 [PMID: 28716818] (IHC, Mouse)
• Melchior , John T, Sawyer , Janet K, Kelley , Kathryn, Shah , Ramesh, Wilson , Martha D, Hantgan , Roy R, Rudel , Lawrence LDL particle core enrichment in cholesteryl oleate increases proteoglycan binding and promotes atherosclerosis. J Lipid Res, 2013-06-26;54(9):2495-503. 2013-06-26 [PMID: 23804810] (ChIP, Human)
• Zhang Y, Mao X, Schwend T, Littlechild S, Conrad G Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases. Invest Ophthalmol Vis Sci, 2013-02-05;54(2):1014-25. 2013-02-05 [PMID: 23322569] (Western Blot, Human)
• Thompson J, Wilson P, Brandewie K, Taneja D, Schaefer L, Mitchell B, Tannock LR Renal accumulation of biglycan and lipid retention accelerates diabetic nephropathy. Am. J. Pathol., 2011-06-30;179(3):1179-87. 2011-06-30 [PMID: 21723246] (IHC-Fr, Mouse)
• Gomez D, Al Haj Zen A, Borges LF, Philippe M, Gutierrez PS, Jondeau G, Michel JB, Vranckx R Syndromic and non-syndromic aneurysms of the human ascending aorta share activation of the Smad2 pathway. J. Pathol., 2009-05-01;218(1):131-42. 2009-05-01 [PMID: 19224541] (IHC-Fr, Human)
• Kitaya K, Yasuo T Dermatan sulfate proteoglycan biglycan as a potential selectin L/CD44 ligand involved in selective recruitment of peripheral blood CD16(-) natural killer cells into human endometrium. J. Leukoc. Biol., 2008-12-16;85(3):391-400. 2008-12-16 [PMID: 19088176] (IHC-P, Western Blot, Human)

Bioinformatics

• Gene Symbol: BGN
• Uniprot:
  • P21810()