Biglycan Antibody

Biglycan was detected in immersion fixed paraffin-embedded sections of human kidney array using Human Biglycan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2667) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

LDL binding to the vessel wall in vitro following treatment with Site B peptide or enzymatic digestion of proteoglycan GAG chains. Tissue sections of carotid arteries from wild‐type mice with intimal hyperplasia were incubated with human LDL. Bound LDL was detected using anti‐apoB antibody. (A) LDL binding to tissue sections pre‐incubated with positively charged SiteB peptide (white circles) or neutrally charged SiteB KE peptide (gray circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). (B) LDL binding to tissue sections pre‐treated with the GAG‐degrading enzymes chondroitinase (white circles) or heparinase (white dot circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). Data was analyzed using Mann–Whitney rank sum test. Graph bars show median values. P < 0.05 is regarded significant, n = 5–8 in each group. (C) Multi‐immunostaining for biglycan (red, left panels) and perlecan (green, right panels) of tissue sections from the injured region (upper panels) and the uninjured region (lower panels) 3 weeks after carotid injury in a wild‐type mouse. Nuclei are stained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28716818), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Biglycan Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Biglycan
  Asp38-Lys368
  Accession # P21810
• Specificity: Detects human Biglycan in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BGN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Biglycan Antibody

• BGN
• Biglycan
• Bone/cartilage proteoglycan I
• bone/cartilage proteoglycan-I
• dermatan sulphate proteoglycan I
• DSPG1
• PGI
• PG-S1
• SLRR1A
• SLRR1Abiglycan proteoglycan
• small leucine-rich protein 1A

Background

Biglycan is a small secreted proteoglycan belonging to the leucine-rich repeat (LRR) protein family. It contains a central 12 LRR repeat domain flanked by small cysteine clusters at either side. Two glycosaminoglycans (GAG) chains are attached near the amino terminus. Biglycan binds BMP-4 and Chordin to regulate BMP-4 signaling. It may also be involved in collagen fiber assembly. Human Biglycan shares 98% amino acid sequence homology with mouse Biglycan, 97% sequence homology with rat and bovine Biglycan and 96% sequence homology with canine and equine Biglycan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Biglycan Antibody (AF2667)(18)

Publications using AF2667

• Kwack, MH;Hamida, OB;Kim, MK;Kim, MK;Sung, YK; Establishment and characterization of matched immortalized human frontal and occipital scalp dermal papilla cell lines from androgenetic alopecia Scientific reports 2023-12-05 [PMID: 38049592] (Immunocytochemistry/ Immunofluorescence, Human)
• LT Hsieh, BS Hall, J Newcombe, TA Mendum, Y Umrania, MJ Deery, WQ Shi, FJ Salguero, RE Simmonds Mycolactone causes catastrophic Sec61-dependent loss of the endothelial glycocalyx and basement membrane: a new indirect mechanism driving tissue necrosis in Mycobacterium ulcerans infection bioRxiv : the preprint server for biology, 2023-02-21;0(0):. 2/21/2023 [PMID: 36865118]
• A Minns, Y Qi, K Yamamoto, K Lee, J Ahnström, S Santamaria The C-terminal domains of ADAMTS1 contain exosites involved in its proteoglycanase activity The Journal of Biological Chemistry, 2023-02-21;0(0):103048. 2023-02-21 [PMID: 36813235] (Western Blot, N/A)
• Daniella dos Santos Courrol, Cristiane Castilho Fernandes da da Silva, Luan Gavião Prado, Rosa Maria Chura-Chambi, Ligia Morganti, Gisele Oliveira de Souza et al. Leptolysin, a Leptospira secreted metalloprotease of the pappalysin family with broad-spectrum activity Frontiers in Cellular and Infection Microbiology 8/23/2022 [PMID: 36081769]
• S Santamaria, DR Martin, X Dong, K Yamamoto, SS Apte, J Ahnström Post-Translational Regulation And Proteolytic Activity Of The Metalloproteinase Adamts8 The Journal of Biological Chemistry, 2021-10-21;0(0):101323. 2021-10-21 [PMID: 34687701] (Western Blot, Human)
• Aysha Ferdoushi, Xiang Li, Nathan Griffin, Sam Faulkner, M. Fairuz B. Jamaluddin, Fangfang Gao et al. Schwann Cell Stimulation of Pancreatic Cancer Cells: A Proteomic Analysis Frontiers in Oncology 8/25/2020 [PMID: 32984024]
• AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases Sci Rep, 2020-07-31;10(1):12912. 2020-07-31 [PMID: 32737331] (Western Blot, Mouse)
• S Kijani, AM Vázquez, M Levin, J Borén, P Fogelstran Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis Physiol Rep, 2017-07-01;5(14):. 2017-07-01 [PMID: 28716818] (IHC, Mouse)
• Josephina A.N. Meester, Geert Vandeweyer, Isabel Pintelon, Martin Lammens, Lana Van Hoorick, Simon De Belder et al. Loss-of-function mutations in the X-linked biglycan gene cause a severe syndromic form of thoracic aortic aneurysms and dissections Genetics in Medicine 2017-04-01 [PMID: 27632686] (Immunohistochemistry, Human)
• Josephina A.N. Meester, Geert Vandeweyer, Isabel Pintelon, Martin Lammens, Lana Van Hoorick, Simon De Belder et al. Loss-of-function mutations in the X-linked biglycan gene cause a severe syndromic form of thoracic aortic aneurysms and dissections Genetics in Medicine 4/1/2017 [PMID: 27632686]
• Sayan Basu, Andrew J. Hertsenberg, Martha L. Funderburgh, Michael K. Burrow, Mary M. Mann, Yiqin Du et al. Human limbal biopsy–derived stromal stem cells prevent corneal scarring Science Translational Medicine 12/10/2014 [PMID: 25504883]
• Melchior , John T, Sawyer , Janet K, Kelley , Kathryn, Shah , Ramesh, Wilson , Martha D, Hantgan , Roy R, Rudel , Lawrence LDL particle core enrichment in cholesteryl oleate increases proteoglycan binding and promotes atherosclerosis. J Lipid Res, 2013-06-26;54(9):2495-503. 2013-06-26 [PMID: 23804810] (ChIP, Human)
• Zhang Y, Mao X, Schwend T, Littlechild S, Conrad G Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases. Invest Ophthalmol Vis Sci, 2013-02-05;54(2):1014-25. 2013-02-05 [PMID: 23322569] (Western Blot, Human)
• Thompson J, Wilson P, Brandewie K, Taneja D, Schaefer L, Mitchell B, Tannock LR Renal accumulation of biglycan and lipid retention accelerates diabetic nephropathy. Am. J. Pathol., 2011-06-30;179(3):1179-87. 2011-06-30 [PMID: 21723246] (IHC-Fr, Mouse)
• Anne Waehre, Bente Halvorsen, Arne Yndestad, Cathrine Husberg, Ivar Sjaastad, Ståle Nygård et al. Lack of Chemokine Signaling through CXCR5 Causes Increased Mortality, Ventricular Dilatation and Deranged Matrix during Cardiac Pressure Overload PLoS ONE 4/18/2011 [PMID: 21533157]
• Maria Vistnes, Anne Waehre, Ståle Nygård, Ivar Sjaastad, Kristin B. Andersson, Cathrine Husberg et al. Circulating cytokine levels in mice with heart failure are etiology dependent Journal of Applied Physiology 5/1/2010 [PMID: 20224000]
• Gomez D, Al Haj Zen A, Borges LF, Philippe M, Gutierrez PS, Jondeau G, Michel JB, Vranckx R Syndromic and non-syndromic aneurysms of the human ascending aorta share activation of the Smad2 pathway. J. Pathol., 2009-05-01;218(1):131-42. 2009-05-01 [PMID: 19224541] (IHC-Fr, Human)
• Kitaya K, Yasuo T Dermatan sulfate proteoglycan biglycan as a potential selectin L/CD44 ligand involved in selective recruitment of peripheral blood CD16(-) natural killer cells into human endometrium. J. Leukoc. Biol., 2008-12-16;85(3):391-400. 2008-12-16 [PMID: 19088176] (IHC-P, Western Blot, Human)

Bioinformatics

• Gene Symbol: BGN
• Uniprot:
  • P21810()