LSD1 Antibody

Western Blot: LSD1 Antibody [NB100-56496] - Analysis of LSD1 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody.

Immunohistochemistry-Paraffin: LSD1 Antibody [NB100-56496] - Analysis of human brain, cortex using this antibody at 10 ug/ml.

Simple Western: LSD1 Antibody [NB100-56496] - Shows a specific band for LSD1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Discontinued

LSD1 Antibody Summary

• Immunogen: A portion of amino acids 800-850 of human LSD1 was used as the immunogen.
• Marker: Nucleus Marker
• Specificity: The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a,CRA _b and CRA _c), rhesus monkey, dog (isoforms 1 and 2), mouse (isoforms 1, 2, 3 and 4) and rat.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDM1A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Simple Western 1:10
  • Western Blot 1-3 ug/ml
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
• Control:

  Jurkat Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for LSD1 Antibody

• amine oxidase (flavin containing) domain 2
• AOF2
• AOF2lysine-specific histone demethylase 1
• BHC110
• BHC110FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
• Flavin-containing amine oxidase domain-containing protein 2
• KIAA0601KDM1
• LSD1
• LSD1BRAF35-HDAC complex protein BHC110
• lysine (K)-specific demethylase 1
• Lysine (K)specific Demethylase 1A
• Lysine (K)-specific Demethylase 1A
• lysine-specific histone demethylase 1A

Background

LSD1 a recently identified, first known lysine-specific histone demethylase, is an 886 amino acid nuclear protein belonging to flavin monoamine oxidase family. It contains a SWIRM domain, a FAD-binding motif and an amine oxidase domain. This protein is ubiquitously expressed and is a component of several histone deacetylase complexes. LSD1 acts as a component of the CoREST and other transcriptional co-repressor complexes and also plays an important role in silencing neuronal-specific genes in non-neuronal cells. It is also known to demethylate Lys-4 of histone H3, a specific tag for epigenetic transcriptional activation. Reports suggest that it plays an important role in stimulating androgen-receptor-dependent transcription converting oxygen to hydrogen peroxide (might use alternative electron acceptors). Along with nuclear FHL2 it serves as a novel biomarker predictive for prostate cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KDM1A
• Entrez:
  • 23028(Human)
  • 99982(Mouse)
  • 500569(Rat)
• Uniprot:
  • O60341(Human)