LRRFIP1 Antibody

Western Blot: LRRFIP1 Antibody [NBP1-71835] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (50 ug) cells. Affinity purified rabbit anti-LRRFIP1 antibody used for WB at 0.04 ug/mL (A) and 0.4 ug/mL (B) and used for IP at 6 ug/mg lysate. LRRFIP1 was also immunoprecipitated by rabbit anti-LRRFIP1 antibodies NBP1-71834 and NBP1-71836, which recognize other epitopes.

Immunocytochemistry/Immunofluorescence: LRRFIP1 Antibody [NBP1-71835] - LRRFIP1 in human fibroblasts (Green). 1:250 dilution in 1X PBS/1% BSA/0.3% Triton X-100. 16 hours, 4C. Image submitted by a verified customer review.

Immunoprecipitation: LRRFIP1 Antibody [NBP1-71835] - LRRFIP1 IP in B-cell whole cell lysate. 2 ug antibody : 500 ug whole cell lysate. Same antibody used at 1:2000 for Western blot. Image submitted by a verified customer review.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

LRRFIP1 Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 500 and 550 of human Leucine Rich Repeat (in FLII) Interacting Protein 1 using the numbering given in entry NP_001131024.1 (GeneID 9208).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LRRFIP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence From verified customer review.
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS, 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LRRFIP1 Antibody

• FLAP-1
• FLIIAP1
• GC-binding factor 2
• GCF2
• GCF-2
• HUFI-1
• leucine rich repeat (in FLII) interacting protein 1
• leucine-rich repeat flightless-interacting protein 1
• LRR FLII-interacting protein 1
• MGC10947
• MGC119738
• MGC119739
• NEDD8-conjugating enzyme
• TRIPTAR RNA-interacting protein

Background

Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for LRRFIP1 Antibody (NBP1-71835) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Verified Customer IP Human 11/20/2018

Summary

• Application: Immunoprecipitation
• Sample Tested: B-cell whole cell lysate
• Species: Human
• Lot: A1

Comments

• Comments: 2ug antibody : 500ug whole cell lysate. Same antibody used 1:2000 for western blot.

reviewed by: Verified Customer ICC Human 01/11/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: fibroblasts
• Species: Human
• Lot: A1

Comments

• Comments: 1:250 dilution in 1X PBS/1% BSA/0.3% Triton X-100. 16 hours, 4C.

reviewed by: Verified Customer WB Human 12/12/2017

Summary

• Application: Western Blot
• Sample Tested: human lymphocytes, whole cell lysate
• Species: Human
• Lot: A1

Comments

• Comments: 25ug. 1:2000 dilution in milk, 16H 4C.

Bioinformatics

• Gene Symbol: LRRFIP1
• Uniprot:
  • NP_001131024.1(Human)