Recombinant Human UBE2M/Ubc12 is a member of the NEDD8-conjugating (E2) enzyme family that receives NEDD8 from a NEDD8-activating (E1) enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UBE2M/Ubc12 concentration of 0.1-1 μM.
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Applications/Dilutions
Dilutions
Enzyme Activity
Theoretical MW
21 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using E2-656 in the following applications:
Ubiquitin-conjugating Enzyme E2M (UBE2M), also known as Ubiquitin-conjugating Enzyme 12 (Ubc12), is a ubiquitously expressed protein with a predicted molecular weight of 20 kDa. UBE2M/Ubc12 is highly conserved, and the human ortholog shares 100% amino acid identity with mouse and rat UBE2M. UBE2M/Ubc12 mediates conjugation of the Ubiquitin-like protein Neural Precursor Cell Expressed, Developmentally Down-regulated 8 (NEDD8) to substrate proteins (1). Neddylation requires the coordinated activities of UBE2M/Ubc12 and the APPBPI/UBA3 heterodimer, a NEDD8-activating (E1) enzyme (2,3). The NEDD8 pathway is essential for the viability of many organisms and plays important roles in morphogenesis and cell cycle progression (4-5). UBE2M/Ubc12-dependent neddylation modulates the levels of p27/Kip1, beta-Catenin, Cdc53/Cul1, and other members of the Cullin family (6-9).
Gong, L. et al. (1999) J. Biol. Chem. 274:12036.
Huang, D.T. et al. (2005) Mol. Cell 17:341.
Ohki, Y. et al. (2009) Biochem. Biophys. Res. Commun. 381:443.
Osaka, F. et al. (2000) EMBO J. 19:3475.
Tateishi, K. et al. (2001) J. Cell Biol. 155:571.
Huang, A.M. et al. (2011) Biochem. Pharmacol. 82:35.
Miller, B.W. et al. (2009) Mol. Syst. Biol. 5:315.
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