Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein, CF

• Reactivity: Hu, Mu, Rt
• Applications: Enzyme Activity
• Format: Carrier-Free

Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein, CF Summary

• Details of Functionality: Recombinant Human UBE2M/Ubc12 is a member of the NEDD8-conjugating (E2) enzyme family that receives NEDD8 from a NEDD8-activating (E1) enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UBE2M/Ubc12 concentration of 0.1-1 μM.
• Source: E. coli-derived UBE2M/Ubc12 protein
  Met1 - Lys183
• Accession #: P61081.1
• Protein/Peptide Type: Recombinant Enzymes
• Gene: UBE2M
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, TCEP and Glycerol.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein, CF

• EC 6.3.2
• EC 6.3.2.-
• hUbc12
• NEDD8 carrier protein
• NEDD8 protein ligase
• UBC12
• UBC12NEDD8-conjugating enzyme Ubc12
• UbcH12
• UBC-RS2
• UBE2M
• ubiquitin carrier protein M
• Ubiquitin-conjugating enzyme E2 M
• ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12)
• ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
• ubiquitin-protein ligase M
• yeast UBC12 homolog

Background

Ubiquitin-conjugating Enzyme E2M (UBE2M), also known as Ubiquitin-conjugating Enzyme 12 (Ubc12), is a ubiquitously expressed protein with a predicted molecular weight of 20 kDa. UBE2M/Ubc12 is highly conserved, and the human ortholog shares 100% amino acid identity with mouse and rat UBE2M. UBE2M/Ubc12 mediates conjugation of the Ubiquitin-like protein Neural Precursor Cell Expressed, Developmentally Down-regulated 8 (NEDD8) to substrate proteins (1). Neddylation requires the coordinated activities of UBE2M/Ubc12 and the APPBPI/UBA3 heterodimer, a NEDD8-activating (E1) enzyme (2,3). The NEDD8 pathway is essential for the viability of many organisms and plays important roles in morphogenesis and cell cycle progression (4-5). UBE2M/Ubc12-dependent neddylation modulates the levels of p27/Kip1, beta-Catenin, Cdc53/Cul1, and other members of the Cullin family (6-9).

1. Gong, L. et al. (1999) J. Biol. Chem. 274:12036. 
2. Huang, D.T. et al. (2005) Mol. Cell 17:341. 
3. Ohki, Y. et al. (2009) Biochem. Biophys. Res. Commun. 381:443.
4. Osaka, F. et al. (2000) EMBO J. 19:3475.
5. Tateishi, K. et al. (2001) J. Cell Biol. 155:571. 
6. Huang, A.M. et al. (2011) Biochem. Pharmacol. 82:35.
7. Miller, B.W. et al. (2009) Mol. Syst. Biol. 5:315.
8. Huang, D.T. et al. (2009) Mol. Cell 33:483.
9. Tanaka, T. et al. (2012) Mol. Oncol. 6:267.

Publications for UBE2M/Ubc12 (E2-656)(7)

Publications using E2-656

• AM Cathcart, GH Bird, TE Wales, HD Herce, EP Harvey, ZJ Hauseman, CE Newman, U Adhikary, MS Prew, T Oo, S Lee, JR Engen, LD Walensky Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer Nat. Chem. Biol., 2020;0(0):. 2020 [PMID: 32807965] (Bioassay, N/A)
• AM Cathcart, GH Bird, TE Wales, HD Herce, EP Harvey, ZJ Hauseman, CE Newman, U Adhikary, MS Prew, T Oo, S Lee, JR Engen, LD Walensky Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer Nat. Chem. Biol., 2020;0(0):. 2020 [PMID: 32807965] (Bioassay, Human)
• ER Wolf, AR Mabry, B Damania, LD Mayo Mdm2-mediated neddylation of pVHL blocks the induction of antiangiogenic factors Oncogene, 2020;0(0):. 2020 [PMID: 32555333] (Bioassay, Human)
• H Zhou, J Lu, L Liu, D Bernard, CY Yang, E Fernandez-, K Chinnaswam, S Layton, J Stuckey, Q Yu, W Zhou, Z Pan, Y Sun, S Wang A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation Nat Commun, 2017;8(1):1150. 2017 [PMID: 29074978] (Bioassay, Human)
• KE Coleman, M Békés, JR Chapman, SB Crist, MJ Jones, BM Ueberheide, TT Huang SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function Elife, 2017;6(0):. 2017 [PMID: 28475037] (Bioassay, Human)
• Huang G, Towe C, Choi L, Yonekawa Y, Bommelje C, Bains S, Rechler W, Hao B, Ramanathan Y, Singh B The ubiquitin-associated (UBA) domain of SCCRO/DCUN1D1 protein serves as a feedback regulator of biochemical and oncogenic activity. J Biol Chem, 2015;290(1):296-309. 2015 [PMID: 25411243] (Bioassay, Human)
• Huang G, Stock C, Bommelje C, Weeda V, Shah K, Bains S, Buss E, Shaha M, Rechler W, Ramanathan S, Singh B SCCRO3 (DCUN1D3) antagonizes the neddylation and oncogenic activity of SCCRO (DCUN1D1). , 2014;0(0):. 2014 [PMID: 25349211] (Human)

Bioinformatics

• Gene Symbol: UBE2M
• Uniprot:
  • P61081.1()