AP2 alpha Antibody (3B5)

Images

 
Western Blot: AP2 alpha Antibody (3B5) [NB100-74359] - Analysis was performed on whole cell extracts (30 ug lysate) of PC-3 (Lane1), A-431 (Lane2), Mouse Placenta (Lane3) and RAW 264.7 (Lane5).
Immunocytochemistry/ Immunofluorescence: AP2 alpha Antibody (3B5) [NB100-74359] - Analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) shows staining in Hela Cells. AP2 staining (green), F-Actin staining with ...read more
Immunohistochemistry-Paraffin: AP2 alpha Antibody (3B5) [NB100-74359] - Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue.
Western Blot: AP2 alpha Antibody (3B5) [NB100-74359] - Figure 1 illustrates Western blot detection of AP-2 from MCF7 cell lysate.
Immunocytochemistry/ Immunofluorescence: AP2 alpha Antibody (3B5) [NB100-74359] - Analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) shows staining in U251 Cells. AP2 staining (green), F-Actin staining with ...read more
Immunocytochemistry/ Immunofluorescence: AP2 alpha Antibody (3B5) [NB100-74359] - Analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) shows staining in MCF-7 Cells. AP2 staining (green), F-Actin staining with ...read more

Product Details

Summary
Reactivity Hu, Mu, ChSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
3B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

AP2 alpha Antibody (3B5) Summary

Immunogen
N-terminus of human AP2a.
Specificity
AP-2 (3B5)
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TFAP2A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:50 - 1:200
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Frozen 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 1:100 - 1:250
Application Notes
In WB: Detects a 45 - 50 kDa protein representing AP-2. Block with BSA only (not milk). IHC-Fr usage was reported in scientific literature (PMID: 24996922).
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-74359 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for AP2 alpha Antibody (3B5)

  • Activating enhancer-binding protein 2-alpha
  • Activator protein 2
  • AP-2 transcription factor
  • AP2-alpha
  • AP2TFAP-2alpha
  • AP-2transcription factor AP-2 alpha (activating enhancer-binding protein 2 alpha)
  • BOFS
  • FLJ51761
  • TFAP2
  • transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
  • transcription factor AP-2-alpha

Background

Activation Protein transcription factor 2 (AP2) is a developmentally regulated transcription factor that recognizes a specific sequence CCCCAGGC or related sequences present in several promoter and enhancer gene regions including the promoters of the AP2 genes themselves. There are three different but highly homologous AP2 isoforms: alpha, beta, and gamma (ERF-1), encoded by three different retinoic acid-responsive genes. Each form of AP2 possesses the DNA binding domain (basic helix-span-helix motif) characteristic of this family of transcription factors. The AP2 proteins are normally expressed in ectodermally derived vertebrate tissues where they are necessary for normal growth and development. The factors have also been implicated in the control of cell proliferation, viral transformation, and oncogenesis. AP2 seems to play in important role in human breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AP2 alpha Antibody (NB100-74359)(2)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TFAP2A
Entrez
Uniprot