CYLD Antibody

Images

 
Western Blot: CYLD Antibody [NB100-56461] - analysis of CYLD in HeLa cell lysate using this antibody.
Immunocytochemistry/ Immunofluorescence: CYLD Antibody [NB100-56461] - Staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: CYLD Antibody [NB100-56461] - Staining of HeLa cell using NB100-56416 (CYLD) at 5 ug/ml. ICC staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.
Simple Western: CYLD Antibody [NB100-56461] - Simple Western lane view shows a specific band for CYLD in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Fe, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CYLD Antibody Summary

Immunogen
A synthetic peptide (DKQTQKLLKVPKGS) corresponding to amino acids 32-45 of human CYLD was used as immunogen (NP_001035877).
Specificity
The amino acid sequence used has 100% identity in human, mouse, chimpanzee, bovine and feline, and 92% homologous in rat. Human CYLD is a 956 amino acid protein and the approximate theoretical size is 105 kD.
Predicted Species
Mouse (100%), Rat (92%), Feline (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYLD
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 5-10 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publication using
NB100-56461 in the following applications:

  • WB
    1 publication

Reactivity Notes

Predicted to react with rat, mouse, bovine, feline, and primate based on homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CYLD Antibody

  • BRSS
  • CDMT
  • CYLD
  • CYLD1
  • CYLD1MFT
  • CYLDI
  • cylindromatosis (turban tumor syndrome)
  • Deubiquitinating enzyme CYLD
  • EAC
  • EC 3.1.2.15
  • EC 3.4.19.12
  • FLJ20180
  • FLJ31664
  • KIAA0849FLJ78684
  • MFT1
  • probable ubiquitin carboxyl-terminal hydrolase CYLD
  • SBS
  • TEM
  • ubiquitin carboxyl-terminal hydrolase CYLD
  • ubiquitin specific peptidase like 2
  • ubiquitin thioesterase CYLD
  • Ubiquitin thiolesterase CYLD
  • Ubiquitin-specific-processing protease CYLD
  • USPL2

Background

CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CYLD Antibody (NB100-56461)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CYLD Antibody (NB100-56461) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CYLD Antibody (NB100-56461)

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Bioinformatics

Gene Symbol CYLD
Entrez
Uniprot