CYLD Antibody - BSA Free

Western Blot: CYLD Antibody [NB100-56461] - Analysis of CYLD in HeLa cell lysate using this antibody.

Immunocytochemistry/Immunofluorescence: CYLD Antibody [NB100-56461] - Staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.

Immunocytochemistry/Immunofluorescence: CYLD Antibody [NB100-56461] - Staining of HeLa cell using this antibody at 5 ug/ml. ICC staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.

Simple Western: CYLD Antibody [NB100-56461] - Simple Western lane view shows a specific band for CYLD in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Bv, Fe, Pm
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CYLD Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CYLD Antibody - BSA Free (NB100-56461) is a polyclonal antibody validated for use in WB, ICC/IF and Simple Western. Anti-CYLD Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide (DKQTQKLLKVPKGS) corresponding to amino acids 32-45 of human CYLD was used as immunogen (NP_001035877).
• Predicted Species: Mouse (100%), Rat (92%), Primate (100%), Feline (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CYLD
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 5-10 ug/ml
  • Simple Western 1:50
  • Western Blot 2-4 ug/ml
• Application Notes: Human CYLD is a 956 amino acid protein and the approximate theoretical size is 105 kD.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 100 kDa
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CYLD Antibody - BSA Free

• BRSS
• CDMT
• CYLD
• CYLD1
• CYLD1MFT
• CYLDI
• cylindromatosis (turban tumor syndrome)
• Deubiquitinating enzyme CYLD
• EAC
• EC 3.1.2.15
• EC 3.4.19.12
• FLJ20180
• FLJ31664
• KIAA0849FLJ78684
• MFT1
• probable ubiquitin carboxyl-terminal hydrolase CYLD
• SBS
• TEM
• ubiquitin carboxyl-terminal hydrolase CYLD
• ubiquitin specific peptidase like 2
• ubiquitin thioesterase CYLD
• Ubiquitin thiolesterase CYLD
• Ubiquitin-specific-processing protease CYLD
• USPL2

Background

CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CYLD Antibody (NB100-56461)(1)

Publication using NB100-56461

• Moranta D, V Regueiro, C March et al. Klebsiella pneumoniae capsule polysaccharide impedes the expression of beta-defensins by airway epithelial cells. Infect & Immun 2010-01-01 [PMID: 20008534] (WB, Human)
  
  Details:
  CYLD (IMG-5815A) WB, Fig 6C, Fig 7A, Fig 7B, A549 human lung carcinoma cells.

Bioinformatics

• Gene Symbol: CYLD
• Entrez:
  • 1540(Human)
  • 74256(Mouse)
  • 312937(Rat)
• Uniprot:
  • Q9NQC7(Human)