Western Blot: CYLD Antibody [NB100-56461] - analysis of CYLD in HeLa cell lysate using this antibody.
Immunocytochemistry/ Immunofluorescence: CYLD Antibody [NB100-56461] - Staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: CYLD Antibody [NB100-56461] - Staining of HeLa cell using NB100-56416 (CYLD) at 5 ug/ml. ICC staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.
Simple Western: CYLD Antibody [NB100-56461] - Simple Western lane view shows a specific band for CYLD in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
A synthetic peptide (DKQTQKLLKVPKGS) corresponding to amino acids 32-45 of human CYLD was used as immunogen (NP_001035877).
The amino acid sequence used has 100% identity in human, mouse, chimpanzee, bovine and feline, and 92% homologous in rat. Human CYLD is a 956 amino acid protein and the approximate theoretical size is 105 kD.
Mouse (100%), Rat (92%), Feline (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our CYLD Antibody and receive a gift card or discount.