Western Blot: CYLD Antibody [NB100-56461] - analysis of CYLD in HeLa cell lysate using this antibody.
Immunocytochemistry/ Immunofluorescence: CYLD Antibody [NB100-56461] - Staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: CYLD Antibody [NB100-56461] - Staining of HeLa cell using NB100-56416 (CYLD) at 5 ug/ml. ICC staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.
Simple Western: CYLD Antibody [NB100-56461] - Simple Western lane view shows a specific band for CYLD in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
A synthetic peptide (DKQTQKLLKVPKGS) corresponding to amino acids 32-45 of human CYLD was used as immunogen (NP_001035877).
The amino acid sequence used has 100% identity in human, mouse, chimpanzee, bovine and feline, and 92% homologous in rat. Human CYLD is a 956 amino acid protein and the approximate theoretical size is 105 kD.
Mouse (100%), Rat (92%), Bovine (100%), Primate (100%), Feline (100%)
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.