LRRFIP1 Antibody

Images

 
Independent Antibodies: Western Blot: LRRFIP1 Antibody [NBP1-71835] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (50 ug) cells. Affinity purified rabbit ...read more
Immunocytochemistry/ Immunofluorescence: LRRFIP1 Antibody [NBP1-71835] - LRRFIP1 in human fibroblasts (Green). 1:250 dilution in 1X PBS/1% BSA/0.3% Triton X-100. 16 hours, 4C. Image submitted by a verified customer ...read more
Immunoprecipitation: LRRFIP1 Antibody [NBP1-71835] - LRRFIP1 IP in B-cell whole cell lysate. 2 ug antibody : 500 ug whole cell lysate. Same antibody used at 1:2000 for Western blot. Image submitted by a verified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

LRRFIP1 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 500 and 550 of human Leucine Rich Repeat (in FLII) Interacting Protein 1 using the numbering given in entry NP_001131024.1 (GeneID 9208).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LRRFIP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence -From verified customer review.
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Reviewed Applications
Read 3 Reviews rated 4.3
using
NBP1-71835 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS, 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LRRFIP1 Antibody

  • FLAP-1
  • FLIIAP1
  • GC-binding factor 2
  • GCF2
  • GCF-2
  • HUFI-1
  • leucine rich repeat (in FLII) interacting protein 1
  • leucine-rich repeat flightless-interacting protein 1
  • LRR FLII-interacting protein 1
  • MGC10947
  • MGC119738
  • MGC119739
  • NEDD8-conjugating enzyme
  • TRIPTAR RNA-interacting protein

Background

Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LRRFIP1 Antibody (NBP1-71835) (0)

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Reviews for LRRFIP1 Antibody (NBP1-71835) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NBP1-71835:
Filter by Applications
IP
(1)
ICC
(1)
WB
(1)
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Filter by Species
Human
(3)
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Images Ratings Applications Species Date Details
Immunoprecipitation LRRFIP1 NBP1-71835
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5
reviewed by:
Verified Customer
IP Human 11/20/2018
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Summary

ApplicationImmunoprecipitation
Sample TestedB-cell whole cell lysate
SpeciesHuman
LotA1

Comments

Comments2ug antibody : 500ug whole cell lysate. Same antibody used 1:2000 for western blot.
Immunocytochemistry LRRFIP1 NBP1-71835
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4
reviewed by:
Verified Customer
ICC Human 01/11/2018
View

Summary

ApplicationImmunocytochemistry
Sample Testedfibroblasts
SpeciesHuman
LotA1

Comments

Comments1:250 dilution in 1X PBS/1% BSA/0.3% Triton X-100. 16 hours, 4C.
Western Blot LRRFIP1 NBP1-71835
Enlarge
4
reviewed by:
Verified Customer
WB Human 12/12/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman lymphocytes, whole cell lysate
SpeciesHuman
LotA1

Comments

Comments25ug. 1:2000 dilution in milk, 16H 4C.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LRRFIP1 Antibody (NBP1-71835) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Verified Customer
11/20/2018
Application: IP
Species: Human

Verified Customer
01/11/2018
Application: ICC
Species: Human

Verified Customer
12/12/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol LRRFIP1
Uniprot