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LRRFIP1 Antibody

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Western Blot: LRRFIP1 Antibody [NBP1-71835] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-LRRFIP1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. LRRFIP1 was also immunoprecipitated by rabbit anti-LRRFIP1 antibodies NBP1-71834 and NBP1-71836, which recognize other epitopes.
Independent Antibodies: Western Blot: LRRFIP1 Antibody [NBP1-71835] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit ...read more
Immunocytochemistry/ Immunofluorescence: LRRFIP1 Antibody [NBP1-71835] - LRRFIP1 in human fibroblasts (Green). 1:250 dilution in 1X PBS/1% BSA/0.3% Triton X-100. 16 hours, 4C. This image was submitted by customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

      

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LRRFIP1 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 500 and 550 of human Leucine Rich Repeat (in FLII) Interacting Protein 1 using the numbering given in entry NP_001131024.1 (GeneID 9208).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LRRFIP1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 2 - 5 ug/mg lysate
Reviewed Applications
Read 1 Review rated 4
using
NBP1-71835 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LRRFIP1 Antibody

  • FLAP-1
  • FLIIAP1
  • GC-binding factor 2
  • GCF2
  • GCF-2
  • HUFI-1
  • leucine rich repeat (in FLII) interacting protein 1
  • leucine-rich repeat flightless-interacting protein 1
  • LRR FLII-interacting protein 1
  • MGC10947
  • MGC119738
  • MGC119739
  • NEDD8-conjugating enzyme
  • TRIPTAR RNA-interacting protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
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NFkB p50 (NLS)
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Western Blot: TRAIP Antibody (2D4) [NBP2-03822] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRAIP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRAIP.
Immunohistochemistry-Paraffin: TRAIP Antibody (2D4) [NBP2-03822] Staining of paraffin-embedded Human liver tissue using anti-TRAIP mouse monoclonal antibody.
Flow Cytometry: TRAIP Antibody (2D4) [NBP2-03822] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRAIP antibody, and then analyzed by flow cytometry.
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Immunohistochemistry: TANK Antibody [NB100-56144] - Immunohistochemical analysis of TANK/I-TRAF1 expression in formalin-fixed, paraffin-embedded normal human colon using NB100-56144 at 1:2000. A-A3, low and high magnification images. Hematoxylin-eosin counterstain.
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EGFR at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
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Western Blot: TRAF-2 Antibody (33A1293) [NB100-56715] - Analysis of TRAF2 in HeLa lysate using TRAF2 antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: TRAF-2 Antibody (33A1293) [NB100-56715] - Formalin-fixed, paraffin-embedded human transitional cell carcinoma of the urinary bladder stained with TRAF2 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
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Applications: WB, IHC, IHC-P
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Western Blot: LRRFIP2 Antibody [NBP2-58998] - Western blot analysis in human cell line RT-4.
Immunocytochemistry/Immunofluorescence: LRRFIP2 Antibody [NBP2-58998] - Staining of human cell line U-2 OS shows localization to plasma membrane & actin filaments.
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EAAT2/GLT1
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Western Blot: RhoA Antibody [NB100-91273] - Rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA: whole serum at 1:2000 dilution. One single band is detected.
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(1 Review)
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Applications: WB, ICC/IF, IHC, IHC-P
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Cip4 Knockout 293T Cell Lysate
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Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035] - Human 721_B, Antibody Dilution: 1.0 ug/ml UBE2M is supported by BioGPS gene expression data to be expressed in 721_B.
Immunocytochemistry/Immunofluorescence: UBE2M/Ubc12 Antibody [NBP1-55035] - Human brain stem cells, antibody concentration 2 ug/ml.
Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035] - Mouse Brain lysate, Antibody Titration: 0.2-1 ug/ml
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Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis using 2 ug/ml on (A) human intestine and 6 ug/ml on (B) mouse intestine and C) rat intestine lysate.
Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Formalin-fixed, paraffin-embedded human skin stained with at 5 ug/ml, peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
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Western Blot: CYLD Antibody [NB100-56461] - analysis of CYLD in HeLa cell lysate using this antibody.
Immunocytochemistry/Immunofluorescence: CYLD Antibody [NB100-56461] - Staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.
Immunocytochemistry/Immunofluorescence: CYLD Antibody [NB100-56461] - Staining of HeLa cell using NB100-56416 (CYLD) at 5 ug/ml. ICC staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.
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LRRFIP1 Antibody
NBP1-71835
LRRFIP1 Antibody
Species: Hu
Applications: WB, ICC/IF, IP

Publications for LRRFIP1 Antibody (NBP1-71835) (0)

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Review for LRRFIP1 Antibody (NBP1-71835) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-71835:
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Immunocytochemistry LRRFIP1 NBP1-71835
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reviewed by:
Anonymous
 ICC Human 01/11/2018
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Summary

ApplicationImmunocytochemistry
Sample Tested fibroblasts
SpeciesHuman
LotA1

Comments

Comments1:250 dilution in 1X PBS/1% BSA/0.3% Triton X-100. 16 hours, 4C.

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Immunocytochemistry LRRFIP1 NBP1-71835
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 Anonymous
01/11/2018
Application: ICC
Species: Human
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Bioinformatics

Gene Symbol LRRFIP1
Uniprot