LRH-1/NR5A2 Antibody (JE30-50)

Western Blot: LRH-1/NR5A2 Antibody (JE30-50) [NBP3-32660] - Western blot analysis of LRH-1/NR5A2 on different lysates with Rabbit anti-LRH-1/NR5A2 antibody (NBP3-32660) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 ug/Lane) Lane 2: 293T cell lysate (15 ug/Lane) Lane 3: HepG2 cell lysate (15 ug/Lane) Lane 4: A549 cell lysate (15 ug/Lane) Lane 5: PANC-1 cell lysate (15 ug/Lane) Lane 6: SW1990 cell lysate (15 ug/Lane) Lane 7: Mouse liver tissue lysate (30 ug/Lane) Lane 8: Rat liver tissue lysate (30 ug/Lane) Lane 9: Mouse brain tissue lysate (30 ug/Lane) Lane 10: Rat brain tissue lysate (30 ug/Lane) Lane 11: Mouse pancreas tissue lysate (30 ug/Lane) Predicted band size: 61 kDa Observed band size: 65 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32660) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: JE30-50
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

LRH-1/NR5A2 Antibody (JE30-50) Summary

• Description: Novus Biologicals Rabbit LRH-1/NR5A2 Antibody (JE30-50) (NBP3-32660) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human LRH-1/NR5A2 aa 442-541 / 541. (Uniprot: O00482)
• Localization: Nucleus.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NR5A2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for LRH-1/NR5A2 Antibody (JE30-50)

• Alpha-1-fetoprotein transcription factor
• B1-binding factor
• b1-binding factor, hepatocyte transcription factor which activates enhancer IIof hepatitis B virus
• B1F
• B1F2
• fetoprotein-alpha 1 (AFP) transcription factor
• FTFLRH1
• FTZ-F1 beta
• FTZ-F1
• FTZ-F1beta
• HB1F
• hB1F-2
• hB1FCYP7A promoter-binding factor
• Hepatocytic transcription factor
• Liver receptor homolog 1
• liver receptor homolog-1
• LRH1
• LRH-1
• LRH-1CPF
• NR5A2
• nuclear receptor NR5A2
• nuclear receptor subfamily 5 group A member 2
• nuclear receptor subfamily 5, group A, member 2

Background

Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR5A2