LMX1A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: LMX1A Antibody - BSA Free [NBP2-41193] - Validation of LMX1A in HeLa Cells.Immunofluorescent analysis of methanol-fixed HeLa cells labeling LMX1A at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody followed by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Validation in Human Skeletal Muscle. Loading: 10 ul of lysates per lane.Antibodies: LMX1A, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Validation in Mouse Skeletal Muscle.Loading: 10 ul of lysates per lane.Antibodies: LMX1A, 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Validation in Rat Skeletal Muscle.Loading: 10 u of lysates per lane.Antibodies: LMX1A, 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Validation of LMX1A in Mouse Brain Tissue.Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-LMX1A antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Validation of LMX1A in Rat Skeletal Muscle Tissue.Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-LMX1A antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Validation of LMX1A in Human Brain Meningioma.Immunohistochemical analysis of paraffin-embedded human brain meningioma using anti-LMX1A antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

LMX1A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit LMX1A Antibody - BSA Free (NBP2-41193) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-LMX1A Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LMX1A. The immunogen is located within amino acids 300 - 350 of LMX1A. Amino Acid Squence: CPQMPGDHMHPYGAEPL
• Specificity: At least two isoforms are known to exist. This antibody is predicted to not cross-react with LMX1B.
• Predicted Species: Chicken (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LMX1A
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1 - 2 ug/mL
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for LMX1A Antibody - BSA Free

• LIM homeobox transcription factor 1, alpha
• LMX1.1

Background

Insulin is produced exclusively by the beta cells in the islets of Langerhans in the pancreas. The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear genes that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. LMX1 is a homeodomain protein that binds an A/T-rich sequence in the insulin promoter and stimulates transcription of insulin (German et al., 1994 [PubMed 7698771]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LMX1A Antibody (NBP2-41193)(2)

Publications using NBP2-41193

• El-Mezayen NS, Attia MM, Shafik MY et al. Reactive astrocytes targeting with oral vitamin A: Efficient neuronal regeneration for Parkinson's disease treatment and reversal of associated liver fibrosis CNS neuroscience & therapeutics 2023-03-22 [PMID: 36949616] (WB, Rat)
• Jacob F, Pather SR, Huang WK et al. Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism Predominates in Choroid Plexus Epithelium Cell Stem Cell 2020-09-21 [PMID: 33010822]

Bioinformatics

• Gene Symbol: LMX1A