Nurr1/NGFI-B beta/NR4A2 Antibody [Unconjugated]

Nurr1/NGFI‑B beta /NR4A2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Nurr1/NGFI‑B beta /NR4A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2156) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Nurr1/NGFI‑B beta /NR4A2 was detected in immersion fixed paraffin-embedded sections of mouse brain (hippocampus) using Goat Anti-Human/Mouse Nurr1/NGFI‑B beta /NR4A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2156) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu, Mu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Nurr1/NGFI-B beta/NR4A2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Nurr1/NGFI‑B beta /NR4A2
  Val332-Lys558
  Accession # Q06219
• Specificity: Detects human and mouse Nurr1/NGFI‑B beta /NR4A2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NR4A2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nurr1/NGFI-B beta/NR4A2 Antibody [Unconjugated]

• human homolog of
• NGFI-B beta
• NOT
• NR4A2
• nuclear receptor subfamily 4, group A, member 2
• Nurr1
• orphan nuclear receptor NR4A2
• Orphan nuclear receptor NURR1
• T-cell nuclear receptor NOT
• TINUR
• transcriptionally inducible nuclear receptor related 1
• Transcriptionally-inducible nuclear receptor

Background

Nurr1 belongs to the nerve growth factor-induced clone B subfamily of nuclear receptor superfamily and is designated NR4A2 in the Unified Nomenclature System for NRs. Nurr1 can function as a ligand-independent transcription factor. Nurr1 also heterodimerizes with retinoid X receptors (RXRs) to function as ligand-regulated transcription factor. Nurr1 is important for specification of dopaminergic neurons that degenerate in Parkinson’s disease. Human and mouse Nurr1 share 99% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nurr1/NGFI-B beta/NR4A2 Antibody (AF2156)(19)

Publications using AF2156

• Shaker, T;Dagpa, GJ;Cattaud, V;Marriott, BA;Sultan, M;Almokdad, M;Jackson, J; A simple and reliable method for claustrum localization across age in mice Molecular brain 2024-02-17 [PMID: 38368400]
• Salamon I, Park Y, Mi�ki? T et al. Celf4 controls mRNA translation underlying synaptic development in the prenatal mammalian neocortex Nat Commun 2023-09-27 [PMID: 37758766]
• Galazo, MJ;Sweetser, DA;Macklis, JD; Tle4 controls both developmental acquisition and early post-natal maturation of corticothalamic projection neuron identity Cell reports 2023-08-09 [PMID: 37561632] (IHC, Mouse)
• Martin Munz, Arjun Bharioke, Georg Kosche, Verónica Moreno-Juan, Alexandra Brignall, Tiago M. Rodrigues et al. Pyramidal neurons form active, transient, multilayered circuits perturbed by autism-associated mutations at the inception of neocortex Cell 2023-04-27 [PMID: 37071993] (Immunohistochemistry, Transgenic Mouse)
• Flavia Correa Turcato, Elliot Wegman, Tao Lu, Nathan Ferguson, Yu Luo Dopaminergic neurons are not a major Sonic hedgehog ligand source for striatal cholinergic or PV interneurons iScience 2022-11-18 [PMID: 36281454]
• Anna Hoerder-Suabedissen, Gabriel Ocana-Santero, Thomas H Draper, Sophie A Scott, Jesse G Kimani, Andrew M Shelton et al. Temporal origin of mouse claustrum and development of its cortical projections Cerebral Cortex 2023-03-21 [PMID: 36104852]
• F Luo, J Wang, Z Zhang, Z You, A Bedolla, F Okwubido-W, LF Huang, J Silver, Y Luo Inhibition of CSPG receptor PTPsigma promotes migration of newly born neuroblasts, axonal sprouting, and recovery from stroke Oncogene, 2022-07-26;40(4):111137. 2022-07-26 [PMID: 35905716] (IHC, Mouse)
• M Shibata, K Pattabiram, B Lorente-Ga, D Andrijevic, SK Kim, N Kaur, SK Muchnik, X Xing, G Santpere, AMM Sousa, N Sestan Regulation of prefrontal patterning and connectivity by retinoic acid Nature, 2021-10-01;0(0):. 2021-10-01 [PMID: 34599305] (IHC, Human, Mouse)
• Evangel Kummari, Shirley X. Guo-Ross, Heath S. Partington, Jennifer Makenzie Nutter, Jeffrey B. Eells Quantitative Immunohistochemistry to Measure Regional Expression of Nurr1 in the Brain and the Effect of the Nurr1 Heterozygous Genotype Frontiers in Neuroanatomy 2021-04-30 [PMID: 33994958]
• Shin-Hwa Tsai, Chih-Yu Tsao, Li-Jen Lee Altered White Matter and Layer VIb Neurons in Heterozygous Disc1 Mutant, a Mouse Model of Schizophrenia Frontiers in Neuroanatomy 2020-12-15 [PMID: 33384588]
• RC Sellnow, K Steece-Col, F Altwal, IM Sandoval, JH Kordower, TJ Collier, CE Sortwell, AR West, FP Manfredsso Striatal Nurr1 Facilitates the Dyskinetic State and Exacerbates Levodopa-Induced Dyskinesia in a Rat Model of Parkinson's Disease J. Neurosci., 2020-04-01;40(18):3675-3691. 2020-04-01 [PMID: 32238479] (IHC, Rat)
• Leslie Ratié, Elodie Desmaris, Fernando García-Moreno, Anna Hoerder-Suabedissen, Alexandra Kelman, Thomas Theil et al. Loss of Dmrt5 Affects the Formation of the Subplate and Early Corticogenesis Cerebral Cortex 2020-05-14 [PMID: 31845734]
• Y Arai, AW Cwetsch, E Coppola, S Cipriani, H Nishihara, H Kanki, Y Saillour, B Freret-Hod, A Dutriaux, N Okada, H Okano, C Dehay, J Nardelli, P Gressens, T Shimogori, G D'Onofrio, A Pierani Evolutionary Gain of Dbx1 Expression Drives Subplate Identity in the Cerebral Cortex Cell Rep, 2019-10-15;29(3):645-658.e5. 2019-10-15 [PMID: 31618633] (IHC-Fr, Human, Mouse)
• K Onishi, A Uyeda, M Shida, T Hirayama, T Yagi, N Yamamoto, N Sugo Genome Stability by DNA polymerase ? in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development J. Neurosci., 2017-08-01;0(0):. 2017-08-01 [PMID: 28765330] (IHC, Mouse)
• La Manno G, Gyllborg D et al. Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells. Cell 6/10/2016 [PMID: 27716510]
• Villaescusa JC, Li B, Toledo EM et al. A PBX1 transcriptional network controls dopaminergic neuron development and is impaired in Parkinson's disease EMBO J. 6/28/2016 [PMID: 27354364]
• Pedraza M, Hoerder-Suabedissen A, Albert-Maestro M, Molnar Z, De Carlos J Extracortical origin of some murine subplate cell populations. Proc Natl Acad Sci U S A, 2014-04-28;111(23):8613-8. 2014-04-28 [PMID: 24778253] (IHC, Mouse)
• Bui Kar Ip, Nadhim Bayatti, Nicholas J. Howard, Susan Lindsay, Gavin J. Clowry The Corticofugal Neuron-Associated Genes ROBO1, SRGAP1, and CTIP2 Exhibit an Anterior to Posterior Gradient of Expression in Early Fetal Human Neocortex Development Cerebral Cortex 2011-06-01 [PMID: 21060114]
• Nomiyama T, Zhao Y, Gizard F, Findeisen HM, Heywood EB, Jones KL, Conneely OM, Bruemmer D Deficiency of the NR4A neuron-derived orphan receptor-1 attenuates neointima formation after vascular injury. Circulation, 2009-01-19;119(4):577-86. 2009-01-19 [PMID: 19153266] (IHC-P, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: NR4A2
• Uniprot:
  • Q06219()