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PDLIM5 Antibody

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Western Blot: PDLIM5 Antibody [NBP1-84843] - Analysis using Anti-PDLIM5 antibody NBP1-84843 (A) shows similar pattern to independent antibody NBP1-84842 (B).
Independent Antibodies: Western Blot: PDLIM5 Antibody [NBP1-84843] - Analysis using Anti-PDLIM5 antibody NBP1-84843 (A) shows similar pattern to independent antibody NBP1-84842 (B).
Immunocytochemistry/ Immunofluorescence: PDLIM5 Antibody [NBP1-84843] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & focal adhesion sites.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human testis shows no positivity in cells in seminiferous ducts.
Western Blot: PDLIM5 Antibody [NBP1-84843] - Analysis in human cell line A-549.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human skeletal muscle shows strong positivity in myocytes.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
Orthogonal Strategies: Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining in human heart muscle and testis tissues.. Corresponding PDLIM5 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

      

Orthogonal Strategies

  

Order Details

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PDLIM5 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PFGSVSSPKVTSIPSPSSAFTPAHATTSSHASPSPVAAVTPPLFAASGLHANANLSADQSPSALSAGKTAVNVPRQPTVTSVCSETSQELAEGQRRGSQGDSKQQNGPPRKHIVERYTEFYHVPTHSDASKK
Specificity
Specificity of human PDLIM5 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDLIM5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:10-1:20
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Publications
Read Publication using NBP1-84843.

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (84%), Rat (84%). Reactivity reported in scientific literature (PMID: 22253135)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PDLIM5 Antibody

  • Enh
  • ENH1
  • ENHLIM
  • Enigma homolog
  • enigma-like LIM domain protein
  • Enigma-like PDZ and LIM domains protein
  • PDZ and LIM domain 5
  • PDZ and LIM domain protein 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: LPP Antibody [NBP1-89549] - Analysis using Anti-LPP antibody NBP1-89549 (A) shows similar pattern to independent antibody NBP1-89555 (B).
Immunocytochemistry/Immunofluorescence: LPP Antibody [NBP1-89549] - Staining of human cell line U-251 MG shows localization to cytosol and focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LPP Antibody [NBP1-89549] - Staining in human smooth muscle and pancreas tissues using anti-LPP antibody. Corresponding LPP RNA-seq data are presented for the same tissues.
Species: Hu, Mu, Rt
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LIM Kinase 1
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Western Blot: LIM Kinase 1 Antibody (3H6) [NBP2-00748] Analysis of extracts (35ug) from 9 different cell lines by using anti-LIM Kinase 1 monoclonal antibody.
Immunocytochemistry/Immunofluorescence: LIM Kinase 1 Antibody (3H6) [NBP2-00748] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM Kinase 1.
Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (3H6) [NBP2-00748] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LIM Kinase 1 mouse monoclonal antibody.
Species: Hu, Mu, Rt, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
CSRP3 Antibody
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Western Blot: CSRP3 Antibody [NBP1-06983] - (0.01ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Simple Western lane view shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiated into motor neurons, loaded at 0.2 mg/mL. A specific band was detected for Islet‑1 at approximately 53 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western blot shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiatied into motor neurons. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Islet‑1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Islet‑1 was detected in immersion fixed iPS2 human induced pluripotent stem cells differentiated to endocrine progenitor cells using Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Western Blot: RPL9 Antibody [NBP1-82853] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: RPL9 Antibody [NBP1-82853] - Staining of mouse cerebellum shows positivity in Purkinje cells.
Immunohistochemistry-Paraffin: RPL9 Antibody [NBP1-82853] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells
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Immunocytochemistry/Immunofluorescence: MARCKS like protein Antibody [NBP2-38160] - Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.
Immunohistochemistry: MARCKS like protein Antibody [NBP2-38160] - Staining of human lymph node shows strong cytoplasmic positivity in both germinal center cells and non-germinal center cells.
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Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody [996/1] NB120-11197AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Permeabilised HL-60 cells stained with Mouse anti Human MUC-2:FITC
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Analysis of Allophycocyanin conjugate of NB120-11197. An intracellular stain was performed on HeLa cells with MUC2 (996/1) antibody NB120-11197APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were fixed with 4% PFA and then permeabli
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(1 Publication)
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Western Blot: LDB1 Antibody [NBP1-85573] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: LDB1 Antibody [NBP1-85573] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
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Immunocytochemistry/Immunofluorescence: Destrin Antibody (2F7) [NBP2-00527] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Destrin.
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Western Blot: RhoD Antibody [NBP1-58359] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml
Immunohistochemistry: RhoD Antibody [NBP1-58359] - Human Liver Tissue Observed Staining: Cytoplasm in sinusoids of liver Primary Antibody Concentration: 1 : 100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Expos
Immunohistochemistry-Paraffin: RhoD Antibody [NBP1-58359] - Human Tonsil Tissue, 4-8ug/ml.
Species: Hu, Mu, Rt, Bv, Ca, Eq, GP
Applications: WB, IHC, IHC-P
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Immunocytochemistry/Immunofluorescence: PDLIM7 Antibody [NBP1-84841] - Staining of human cell line U-251 MG shows localization to actin filaments and focal adhesion sites. Antibody staining is shown in green.
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Immunohistochemistry-Paraffin: LIM1 Antibody (2D5) [NBP2-01926] - Staining of paraffin-embedded Human prostate tissue using anti-LIM1 mouse monoclonal antibody.
(3 Publications)
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Applications: WB, Flow, ICC/IF, IHC, IHC-P
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Western Blot: LDB2 Antibody [NB600-480] - Analysis of a 43-kDa band corresponding to LDB2 in a lysates prepared from human kidney (lane 1) and mouse spleen (lane 2) tissues. Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LDB2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
Immunohistochemistry: LDB2 Antibody [NB600-480] - Used at a 5 ug/ml to detect LDB2 in human brain cortex tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Tissue was formalin-fixed and paraffin embedded.
Species: Hu, Mu, Rt, Ca, Ch, Cz
Applications: WB, ELISA, IHC, IHC-P
HIC5/TGFB1I1
Antibody [Unconjugate
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Western blot shows lysates of MCF‑7 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HIC5/TGFB1I1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5626) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIC5/TGFB1I1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HIC5/TGFB1I1 was detected in immersion fixed PC‑3 human prostate cancer cell line, unstimulated (upper panel) or stimulated with 10 ng/mL Recombinant Human BMP-4 (Catalog # 314-BP; lower panel), using Goat Anti-Human HIC5/TGFB1I1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5626) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm (unstimulated) and nuclei (stimulated). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Western Blot: FHL1 Antibody (3G7) [NBP2-02654] Analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.
Immunocytochemistry/Immunofluorescence: FHL1 Antibody (3G7) [NBP2-02654] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1.
Flow Cytometry: FHL1 Antibody (3G7) [NBP2-02654] - Analysis of Jurkat cells, using anti-FHL1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
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Applications: WB, Flow, ICC/IF
CSRP1 Antibody
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Western blot shows lysates of ME-180 human cervical epithelial carcinoma cell line, HT-2 mouse T cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse/Rat CSRP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human CSRP1, CSRP2, CSRP3 (5 ng/lane) were included. A specific band for CSRP1 was detected at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu, Mu, Rt
Applications: WB
FHL2 Antibody
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Western Blot: FHL2 Antibody [NBP1-84978] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: FHL2 Antibody [NBP1-84978] - Immunofluorescent staining of human cell line U-2 OS shows localization to actin filaments & focal adhesion sites.
Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-84978] - Staining of human kidney.
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Gelsolin/GSN
Antibody
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Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term.
Immunocytochemistry/Immunofluorescence: Gelsolin/GSN Antibody [NBP1-33177] - Methanol-fixed HeLa, using Gelsolin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2000.
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody [NBP1-33177] - H1299 xenograft, using Gelsolin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for PDLIM5 Antibody (NBP1-84843)(1)


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Bioinformatics

Gene Symbol PDLIM5