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PDLIM5 Antibody

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Western Blot: PDLIM5 Antibody [NBP1-84843] - Analysis using Anti-PDLIM5 antibody NBP1-84843 (A) shows similar pattern to independent antibody NBP1-84842 (B).
Independent Antibodies: Western Blot: PDLIM5 Antibody [NBP1-84843] - Analysis using Anti-PDLIM5 antibody NBP1-84843 (A) shows similar pattern to independent antibody NBP1-84842 (B).
Immunocytochemistry/ Immunofluorescence: PDLIM5 Antibody [NBP1-84843] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & focal adhesion sites.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human testis shows no positivity in cells in seminiferous ducts.
Western Blot: PDLIM5 Antibody [NBP1-84843] - Analysis in human cell line A-549.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human skeletal muscle shows strong positivity in myocytes.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
Orthogonal Strategies: Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining in human heart muscle and testis tissues.. Corresponding PDLIM5 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

      

Orthogonal Strategies

  

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PDLIM5 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PFGSVSSPKVTSIPSPSSAFTPAHATTSSHASPSPVAAVTPPLFAASGLHANANLSADQSPSALSAGKTAVNVPRQPTVTSVCSETSQELAEGQRRGSQGDSKQQNGPPRKHIVERYTEFYHVPTHSDASKK
Specificity
Specificity of human PDLIM5 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDLIM5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:10-1:20
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PDLIM5 Protein (NBP1-84843PEP)
Publications
Read Publication using NBP1-84843.

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (84%), Rat (84%). Reactivity reported in scientific literature (PMID: 22253135)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PDLIM5 Antibody

  • Enh
  • ENH1
  • ENHLIM
  • Enigma homolog
  • enigma-like LIM domain protein
  • Enigma-like PDZ and LIM domains protein
  • PDZ and LIM domain 5
  • PDZ and LIM domain protein 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: LPP Antibody [NBP1-89549] - Analysis using Anti-LPP antibody NBP1-89549 (A) shows similar pattern to independent antibody NBP1-89555 (B).
Immunocytochemistry/Immunofluorescence: LPP Antibody [NBP1-89549] - Staining of human cell line U-251 MG shows localization to cytosol and focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LPP Antibody [NBP1-89549] - Staining in human smooth muscle and pancreas tissues using anti-LPP antibody. Corresponding LPP RNA-seq data are presented for the same tissues.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
LIM Kinase 1
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H00003984-M01
Western Blot: LIM Kinase 1 Antibody (1A8) [H00003984-M01] - LIMK1 monoclonal antibody (M01), clone 1A8. Analysis of LIMK1 expression in Raw 264.7.
Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (1A8) [H00003984-M01] - Analysis of monoclonal antibody to LIMK1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.
Western Blot: LIM Kinase 1 Antibody (1A8) [H00003984-M01] - LIMK1 monoclonal antibody (M01), clone 1A8. Analysis of LIMK1 expression in PC-12.
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
CSRP3 Antibody
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Western Blot: CSRP3 Antibody [NBP1-06983] - (0.01ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP, PEP-ELISA
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Simple Western lane view shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiated into motor neurons, loaded at 0.2 mg/mL. A specific band was detected for Islet‑1 at approximately 53 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western blot shows lysates of JOY6 human induced pluripotent stem cells undifferentiated and differentiatied into motor neurons. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Islet‑1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Islet‑1 was detected in immersion fixed iPS2 human induced pluripotent stem cells differentiated to endocrine progenitor cells using Goat Anti-Human Islet‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1837) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Species: Hu
Applications: WB, Simple Western, ICC
RPL9 Antibody
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Western Blot: RPL9 Antibody [NBP1-82853] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: RPL9 Antibody [NBP1-82853] - Staining of mouse cerebellum shows positivity in Purkinje cells.
Immunohistochemistry-Paraffin: RPL9 Antibody [NBP1-82853] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells
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Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
MARCKS like
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Western Blot: MARCKS like protein Antibody (8C8) [H00065108-M02] - Analysis of MARCKSL1 expression in transfected 293T cell line by MARCKSL1 monoclonal antibody (M02), clone 8C8.Lane 1: MARCKSL1 transfected lysate(19.398 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: MARCKS like protein Antibody (8C8) [H00065108-M02] - Analysis of monoclonal antibody to MARCKSL1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
Sandwich ELISA: MARCKS like protein Antibody (8C8) [H00065108-M02] - Detection limit for recombinant GST tagged MARCKSL1 is approximately 0.1ng/ml as a capture antibody.
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
MUC2 Antibody
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Western Blot: MUC2 Antibody (CCP58) [NBP2-25221] - MDA MB 231 cells and HCC827 human non-small cell lung cancer cell line lysates. Image from verified customer review.
Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC2 Antibody (CCP58).
Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-fixed, paraffin-embedded normal human intestine (10X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.
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(3 Publications)
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Western Blot: LDB1 Antibody [NBP1-31362] - Wild-type (WT) and LDB1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: LDB1 Antibody [NBP1-31362] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LDB1 protein stained by LDB1 antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - mouse muscle.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
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Western Blot: Destrin Antibody (2F7) [NBP2-00527] Analysis of extracts (35ug) from 9 different cell lines by using anti-Destrin monoclonal antibody.
Immunocytochemistry/Immunofluorescence: Destrin Antibody (2F7) [NBP2-00527] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Destrin.
Immunohistochemistry-Paraffin: Destrin Antibody (2F7) [NBP2-00527] - Staining of paraffin-embedded Human prostate tissue using anti-Destrin mouse monoclonal antibody.
Species: Hu, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
RhoD Antibody
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Western Blot: RhoD Antibody [NBP1-58359] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml
Immunohistochemistry: RhoD Antibody [NBP1-58359] - Human Liver Tissue Observed Staining: Cytoplasm in sinusoids of liver Primary Antibody Concentration: 1 : 100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Expos
Immunohistochemistry-Paraffin: RhoD Antibody [NBP1-58359] - Human Tonsil Tissue, 4-8ug/ml.
Species: Hu
Applications: WB, IHC, IHC-P
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Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
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Western Blot: LHX3 Antibody [NBP2-84135] - WB Suggested Anti-LHX3 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysate
Immunohistochemistry: LHX3 Antibody [NBP2-84135] - Human Heart
Immunohistochemistry: LHX3 Antibody [NBP2-84135] - Human Lung
Species: Hu
Applications: WB, IHC, IHC-P
PDLIM7 Antibody
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Western Blot: PDLIM7 Antibody [NBP1-84841] - Analysis using Anti-PDLIM7 antibody NBP1-84841 (A) shows similar pattern to independent antibody NBP2-58734 (B).
Immunocytochemistry/Immunofluorescence: PDLIM7 Antibody [NBP1-84841] - Staining of human cell line U-251 MG shows localization to actin filaments and focal adhesion sites. Antibody staining is shown in green.
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(2 Publications)
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Applications: WB, ICC/IF, IHC, IHC-P
LIM1 Antibody
(OTI2D5)
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Western Blot: LIM1 Antibody (2D5) [NBP2-01926] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIM1.
Immunocytochemistry/Immunofluorescence: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM1.
Immunohistochemistry-Paraffin: LIM1 Antibody (2D5) [NBP2-01926] - Staining of paraffin-embedded Human prostate tissue using anti-LIM1 mouse monoclonal antibody.
(3 Publications)
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
LDB2 Antibody
NB600-480
Western Blot: LDB2 Antibody [NB600-480] - Analysis of a 43-kDa band corresponding to LDB2 in a lysates prepared from human kidney (lane 1) and mouse spleen (lane 2) tissues. Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LDB2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
Immunohistochemistry: LDB2 Antibody [NB600-480] - Used at a 5 ug/ml to detect LDB2 in human brain cortex tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Tissue was formalin-fixed and paraffin embedded.
Species: Hu, Mu, Rt, Ca, Ch, Pm
Applications: WB, ELISA, IHC, IHC-P
HIC5/TGFB1I1
Antibody [Unconjugate
AF5626
Western blot shows lysates of MCF‑7 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HIC5/TGFB1I1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5626) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIC5/TGFB1I1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HIC5/TGFB1I1 was detected in immersion fixed PC‑3 human prostate cancer cell line, unstimulated (upper panel) or stimulated with 10 ng/mL Recombinant Human BMP-4 (Catalog # 314-BP; lower panel), using Goat Anti-Human HIC5/TGFB1I1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5626) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm (unstimulated) and nuclei (stimulated). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Species: Hu
Applications: WB, ICC
FHL1 Antibody
(767418)
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Western blot shows lysates of human skeletal muscle tissue and human heart tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human FHL1 Monoclonal Antibody (Catalog # MAB5938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FHL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FHL1 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Mouse Anti-Human FHL1 Monoclonal Antibody (Catalog # MAB5938) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter­stained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Publication)
Species: Hu
Applications: WB, ICC
CSRP1 Antibody
AF5739
Western blot shows lysates of ME-180 human cervical epithelial carcinoma cell line, HT-2 mouse T cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse/Rat CSRP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human CSRP1, CSRP2, CSRP3 (5 ng/lane) were included. A specific band for CSRP1 was detected at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu, Mu, Rt
Applications: WB
FHL2 Antibody
NBP1-84978
Western Blot: FHL2 Antibody [NBP1-84978] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: FHL2 Antibody [NBP1-84978] - Immunofluorescent staining of human cell line U-2 OS shows localization to actin filaments & focal adhesion sites.
Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-84978] - Staining of human liver shows low positivity in hepatocytes as expected.
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Gelsolin/GSN
Antibody
NBP1-33177
Western Blot: Gelsolin/GSN Antibody [NBP1-33177] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term.
Immunocytochemistry/Immunofluorescence: Gelsolin/GSN Antibody [NBP1-33177] - Methanol-fixed HeLa, using Gelsolin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2000.
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody [NBP1-33177] - H1299 xenograft, using Gelsolin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for PDLIM5 Antibody (NBP1-84843)(1)


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Bioinformatics

Gene Symbol PDLIM5