PDLIM5 Antibody

Images

 
Independent Antibodies: Western Blot: PDLIM5 Antibody [NBP1-84843] - Analysis using Anti-PDLIM5 antibody NBP1-84843 (A) shows similar pattern to independent antibody NBP1-84842 (B).
Immunocytochemistry/ Immunofluorescence: PDLIM5 Antibody [NBP1-84843] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & focal adhesion sites.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human testis shows no positivity in cells in seminiferous ducts.
Western Blot: PDLIM5 Antibody [NBP1-84843] - Analysis in human cell line A-549.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human skeletal muscle shows strong positivity in myocytes.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
Orthogonal Strategies: Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining in human heart muscle and testis tissues.. Corresponding PDLIM5 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PDLIM5 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PFGSVSSPKVTSIPSPSSAFTPAHATTSSHASPSPVAAVTPPLFAASGLHANANLSADQSPSALSAGKTAVNVPRQPTVTSVCSETSQELAEGQRRGSQGDSKQQNGPPRKHIVERYTEFYHVPTHSDASKK
Specificity
Specificity of human PDLIM5 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDLIM5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:10-1:20
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Publications
Read Publication using NBP1-84843.

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (84%), Rat (84%). Reactivity reported in scientific literature (PMID: 22253135)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PDLIM5 Antibody

  • Enh
  • ENH1
  • ENHLIM
  • Enigma homolog
  • enigma-like LIM domain protein
  • Enigma-like PDZ and LIM domains protein
  • PDZ and LIM domain 5
  • PDZ and LIM domain protein 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDLIM5 Antibody (NBP1-84843)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDLIM5 Antibody (NBP1-84843) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PDLIM5