Lipocalin-2/NGAL Antibody

Western Blot: Lipocalin-2/NGAL Antibody [NBP1-45682] - Lane 1. Mouse recombinant Lipocalin-2 / LCN2-2 ng Lane 2. Mouse recombinant Lipocalin-2 / LCN2-10 ng Lane 3. Mouse recombinant Lipocalin-2 / LCN2-50 ng

Immunocytochemistry/Immunofluorescence: Lipocalin-2/NGAL Antibody [NBP1-45682] - Immunofluorescence analysis of 3T3 cells using Anti-Lipocalin-2/NGAL

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Lipocalin-2/NGAL Antibody Summary

• Description: This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab63929)
• Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 40 of mouse Lipocalin 2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LCN2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 0.5 - 4 ug/mL
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 30% glycerol, 0.5% BSA
• Preservative: 0.01% Thimerosal
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for Lipocalin-2/NGAL Antibody

• 24p3
• 25 kDa alpha-2-microglobulin-related subunit of MMP-9
• HNL
• LCN2
• lipocalin 2 (oncogene 24p3)
• lipocalin 2
• Lipocalin2
• Lipocalin-2
• migration-stimulating factor inhibitor
• MSFI
• neutrophil gelatinase-associated lipocalin
• NGAL
• NGALlipocalin-2
• Oncogene 24p3
• p25
• Siderocalin
• Uterocalin

Background

Lipocalin 2 is a member of the lipocalin family which encompass more than 25 members (including proteins like retinol binding protein, a1 microglobulin, b lactoglobulin, apolipoprotein D and odorant binding protein). The members of the lipocalin family are characterized by their ability to bind small lipophilic substances in their hydrophobic core, and thereby serve as transporters of substances. Pig NGAL may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically, from renal tubular cells in response to ischemic or nephrotoxic injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Publications for Lipocalin-2/NGAL Antibody (NBP1-45682)(1)

Publication using NBP1-45682

• Ma S, Xu H, Huang W et al. Chrysophanol Relieves Cisplatin-Induced Nephrotoxicity via Concomitant Inhibition of Oxidative Stress, Apoptosis, and Inflammation Frontiers in Physiology 2021-09-29 [PMID: 34658905] (Immunohistochemistry)

Bioinformatics

• Gene Symbol: LCN2
• Entrez:
  • 3934(Human)
  • 16819(Mouse)
  • 170496(Rat)
• Uniprot:
  • P11672()