Lipase A Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Lipase A Antibody [NBP2-19360] - HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: LAL stained by LAL antibody [N3C3] diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: Lipase A Antibody [NBP2-19360] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 10% SDS PAGE gel, diluted at 1:1000.

Western Blot: Lipase A Antibody [NBP2-19360] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LAL antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.w

Western Blot: Lipase A Antibody [NBP2-19360] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Lipase A antibody [N3C3] (NBP2-19360) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Lipase A Antibody [NBP2-19360] -Mouse kidney.LAL stained by LAL antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Lipase A Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Lipase A. The exact sequence is proprietary.
• Localization: Lysosome
• Predicted Species: Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LIPA
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Lipase A Antibody - BSA Free

• Acid cholesteryl ester hydrolase
• CESD
• Cholesteryl esterase
• EC 3.1.1
• EC 3.1.1.13
• LALCESDcholesterol ester hydrolase
• LIPA
• Lipase A
• lipase A, lysosomal acid, cholesterol esterase
• lysosomal acid lipase
• lysosomal acid lipase/cholesteryl ester hydrolase
• Sterol esterase

Background

This gene encodes lipase A, the lysosomal acid lipase (also known as cholesterol ester hydrolase). This enzyme functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides. Mutations in this gene can result in Wolman disease and cholesteryl ester storage disease. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LIPA
• Uniprot:
  • P38571(Human)