LIF Antibody [Unconjugated]

Images

 
Recombinant Mouse LIF (Catalog # 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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LIF Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse LIF
Specificity
Detects mouse LIF in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than
5% cross‑reactivity with recombinant human (rh) LIF is observed. In direct ELISAs, no cross‑reactivity with rhCNTF, recombinant mouse (rm) G-CSF, rmIL-6, and rmOSM is observed.  
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LIF
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Neutralization 10-60 ng/mL
Publications
Read Publications using
AF449 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIF Antibody [Unconjugated]

  • CDF
  • D factor
  • DIA
  • differentiation inhibitory activity
  • differentiation stimulating factor
  • Differentiation-stimulating factor
  • Emfilermin
  • HILDAcholinergic differentiation factor
  • leukemia inhibitory factor (cholinergic differentiation factor)
  • leukemia inhibitory factor
  • LIF
  • Melanoma-derived LPL inhibitor
  • MLPLI

Background

Leukemia inhibitory factor (LIF) was initially identified as a factor that inhibited the proliferation and induced the differentiation to macrophages of the murine myeloid leukemic cell line M1. Subsequent to its purification and molecular cloning, LIF was recognized to be a pleiotropic factor with multiple effects on both hematopoietic and non-hematopoietic cells. LIF has overlapping biological functions with OSM, IL-6, IL-11 and CNTF. All these cytokines utilize gp130 as a component in their signal transducing receptor complexes. Mouse LIF cDNA encodes a 203 amino acid residue polypeptide with a 23 amino acid signal peptide that is cleaved to yield a 180 amino acid mature mouse LIF. Native human and mouse LIF are highly glycosylated monomeric proteins. Both human and murine LIF protein sequences have multiple potential N- and O-linked glycosylation sites and six conserved cysteine residues that are involved in three intramolecular disulfide bridges.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Mu
Applications: WB, B/N

Publications for LIF Antibody (AF449)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ELISA Development, In vivo, Neut, WB.


Filter By Application
ELISA Development
(1)
In vivo
(2)
Neut
(2)
WB
(1)
All Applications
Filter By Species
Mouse
(6)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF449 Applications Species
H Won, D Moreira, C Gao, P Duttagupta, X Zhao, E Manuel, D Diamond, YC Yuan, Z Liu, J Jones, M D'Apuzzo, S Pal, M Kortylewsk TLR9 expression and secretion of LIF by prostate cancer cells stimulates accumulation and activity of polymorphonuclear MDSCs J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 28533357] (Neut,, Mouse) Neut Mouse
Moidunny S, Vinet J Adenosine A2B receptor-mediated leukemia inhibitory factor release from astrocytes protects cortical neurons against excitotoxicity. J Neuroinflammation, 2012;9(1):198. 2012 [PMID: 22894638] (ELISA Development,, Mouse) ELISA Development Mouse
Quinton LJ, Mizgerd JP, Hilliard KL Leukemia inhibitory factor signaling is required for lung protection during pneumonia. J. Immunol., 2012;188(12):6300-8. 2012 [PMID: 22581855] (In vivo,, Mouse) In vivo Mouse
Cao W, Yang Y, Wang Z, Liu A, Fang L, Wu F, Hong J, Shi Y, Leung S, Dong C, Zhang JZ Leukemia inhibitory factor inhibits T helper 17 cell differentiation and confers treatment effects of neural progenitor cell therapy in autoimmune disease. Immunity, 2011;35(2):273-84. 2011 [PMID: 21835648] (In vivo,, Mouse) In vivo Mouse
Ishibashi T, Dakin KA, Stevens B, Lee PR, Kozlov SV, Stewart CL, Fields RD Astrocytes promote myelination in response to electrical impulses. Neuron, 2006;49(6):823-32. 2006 [PMID: 16543131] (WB,, Mouse) WB Mouse
Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice. Arch. Dermatol. Res., 2001;293(10):508-14. 2001 [PMID: 11820727] (Neut,, Mouse) Neut Mouse

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Blogs on LIF.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

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Bioinformatics

Gene Symbol LIF
Entrez