LIF Antibody [Unconjugated]


Recombinant Mouse LIF (Catalog # 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). more

Product Details

Reactivity MuSpecies Glossary
Applications WB, B/N

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LIF Antibody [Unconjugated] Summary

E. coli-derived recombinant mouse LIF
Detects mouse LIF in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than
5% cross‑reactivity with recombinant human (rh) LIF is observed. In direct ELISAs, no cross‑reactivity with rhCNTF, recombinant mouse (rm) G-CSF, rmIL-6, and rmOSM is observed.  
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.1 ug/mL
  • Neutralization 10-60 ng/mL
Read Publications using
AF449 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIF Antibody [Unconjugated]

  • CDF
  • D Factor
  • DIA
  • differentiation inhibitory activity
  • differentiation stimulating factor
  • Differentiation-stimulating factor
  • Emfilermin
  • HILDAcholinergic differentiation factor
  • leukemia inhibitory factor (cholinergic differentiation factor)
  • leukemia inhibitory factor
  • LIF
  • Melanoma-derived LPL inhibitor


Leukemia inhibitory factor (LIF) was initially identified as a factor that inhibited the proliferation and induced the differentiation to macrophages of the murine myeloid leukemic cell line M1. Subsequent to its purification and molecular cloning, LIF was recognized to be a pleiotropic factor with multiple effects on both hematopoietic and non-hematopoietic cells. LIF has overlapping biological functions with OSM, IL-6, IL-11 and CNTF. All these cytokines utilize gp130 as a component in their signal transducing receptor complexes. Mouse LIF cDNA encodes a 203 amino acid residue polypeptide with a 23 amino acid signal peptide that is cleaved to yield a 180 amino acid mature mouse LIF. Native human and mouse LIF are highly glycosylated monomeric proteins. Both human and murine LIF protein sequences have multiple potential N- and O-linked glycosylation sites and six conserved cysteine residues that are involved in three intramolecular disulfide bridges.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LIF Antibody (AF449)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Bioassay, ELISA Development, Neutralization, Western Blot.

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ELISA Development
Western Blot
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Showing Publications 1 - 7 of 7.
Publications using AF449 Applications Species
Y Tang, S Xiong, P Yu, F Liu, L Cheng Direct Conversion of Mouse Fibroblasts into Neural Stem Cells by Chemical Cocktail Requires Stepwise Activation of Growth Factors and Nup210 Cell Rep, 2018;24(5):1355-1362.e3. 2018 [PMID: 30067988] (Bioassay, Mouse) Bioassay Mouse
H Won, D Moreira, C Gao, P Duttagupta, X Zhao, E Manuel, D Diamond, YC Yuan, Z Liu, J Jones, M D'Apuzzo, S Pal, M Kortylewsk TLR9 expression and secretion of LIF by prostate cancer cells stimulates accumulation and activity of polymorphonuclear MDSCs J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 28533357] (Neutralization, Mouse) Neutralization Mouse
Moidunny S, Vinet J Adenosine A2B receptor-mediated leukemia inhibitory factor release from astrocytes protects cortical neurons against excitotoxicity. J Neuroinflammation, 2012;9(1):198. 2012 [PMID: 22894638] (ELISA Development, Mouse) ELISA Development Mouse
Quinton LJ, Mizgerd JP, Hilliard KL Leukemia inhibitory factor signaling is required for lung protection during pneumonia. J. Immunol., 2012;188(12):6300-8. 2012 [PMID: 22581855] (Neutralization, Mouse) Neutralization Mouse
Cao W, Yang Y, Wang Z, Liu A, Fang L, Wu F, Hong J, Shi Y, Leung S, Dong C, Zhang JZ Leukemia inhibitory factor inhibits T helper 17 cell differentiation and confers treatment effects of neural progenitor cell therapy in autoimmune disease. Immunity, 2011;35(2):273-84. 2011 [PMID: 21835648] (Neutralization, Mouse) Neutralization Mouse
Ishibashi T, Dakin KA, Stevens B, Lee PR, Kozlov SV, Stewart CL, Fields RD Astrocytes promote myelination in response to electrical impulses. Neuron, 2006;49(6):823-32. 2006 [PMID: 16543131] (Western Blot, Mouse) Western Blot Mouse
Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice. Arch. Dermatol. Res., 2001;293(10):508-14. 2001 [PMID: 11820727] (Neutralization, Mouse) Neutralization Mouse

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Biotin BAF449

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Gene Symbol LIF