LIFR alpha Antibody [Unconjugated]


Recombinant Human LIF stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by 0.3 ng/mL Recombinant Human LIF is neutralized (green more

Product Details

Reactivity HuSpecies Glossary
Applications WB, B/N

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LIFR alpha Antibody [Unconjugated] Summary

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LIF R alpha
Accession # P42702
Detects human LIF R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 7% cross-reactivity with recombinant mouse LIF R alpha is observed.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Neutralization 6-36 ug/mL
  • Western Blot 0.1 ug/mL
Read Publications using
AF-249-NA in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIFR alpha Antibody [Unconjugated]

  • CD118 antigen
  • CD118
  • FLJ98106
  • FLJ99923
  • leukemia inhibitory factor receptor alpha
  • leukemia inhibitory factor receptor
  • LIF R alpha
  • LIF R beta
  • LIF receptor
  • LIFR alpha
  • LIFR
  • LIF-R
  • LIFRa
  • SJS2
  • STWS
  • SWS


The activities of the pleiotropic cytokine LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha  subunit (LIF R alpha , also known as LIF R beta and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex. The gp130 subunit was first described as the signal transducing subunit of the high-affinity IL-6 receptor complex. Besides LIF, the high-affinity heterodimeric LIF receptor complex has been shown to mediate the activities of oncostatin M (OSM), cardiotrophin-1 and ciliary neurotrophic factor (CNTF).

Human LIF R alpha  cDNA encodes a 1097 amino acid (aa) residue precursor type I membrane protein with a 44 aa residue signal peptide, a 789 aa residue extracellular domain, a 26 aa residue transmembrane domain, and a 238 aa residue cytoplasmic domain. LIF R alpha is a member of the cytokine receptor family and has extensive homology to gp130. The extracellular domain of LIF R alpha  has two cytokine receptor domains and three fibronectin type III repeats. In mouse, mRNAs encoding a soluble LIF R alpha and lacking transmembrane and intracellular domains, have been isolated. Soluble LIF R alpha has been shown to bind LIF and has LIF antagonistic activity.

  1. Bazan, J.F. (1990) Proc. Natl. Acad. Sci. USA 87:6934.
  2. Gearing, D.P. (1994) Guidebook to Cytokines and Their Receptors, Academic Press, p130.
  3. Pennica D. et al. (1995) J. Biol. Chem. 270:10915.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LIFR alpha Antibody (AF-249-NA)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ELISA Development, ICC, IHC-Fr, IHC-P, Neutralization, Western Blot.

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Western Blot
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF-249-NA Applications Species
H Lörchner, JM Adrian-Seg, C Waechter, R Wagner, ME Góes, N Brachmann, K Sreenivasa, A Wietelmann, S Günther, N Doll, T Braun, J Pöling Concomitant Activation of OSM and LIF Receptor by a Dual-Specific hlOSM Variant Confers Cardioprotection after Myocardial Infarction in Mice International Journal of Molecular Sciences, 2021-12-29;23(1):. 2021-12-29 [PMID: 35008777] (Western Blot, Mouse) Western Blot Mouse
Salm F, Dimitrova V, von Bueren A, Cwiek P, Rehrauer H, Djonov V, Anderle P, Arcaro A The Phosphoinositide 3-Kinase p110alpha Isoform Regulates Leukemia Inhibitory Factor Receptor Expression via c-Myc and miR-125b to Promote Cell Proliferation in Medulloblastoma. PLoS ONE, 2015-04-27;10(4):e0123958. 2015-04-27 [PMID: 25915540] (Neutralization, Human) Neutralization Human
Chollangi , Srinivas, Mather , Timothy, Rodgers , Karla K, Ash , John D A unique loop structure in oncostatin M determines binding affinity toward oncostatin M receptor and leukemia inhibitory factor receptor. J Biol Chem, 2012-07-24;287(39):32848-59. 2012-07-24 [PMID: 22829597] (ELISA Development, Human) ELISA Development Human
Aghajanova L, Altmae S, Bjuresten K, Hovatta O, Landgren BM, Stavreus-Evers A Disturbances in the LIF pathway in the endometrium among women with unexplained infertility. Fertil. Steril., 2008-08-05;91(6):2602-10. 2008-08-05 [PMID: 18684446] (IHC-P, Human) IHC-P Human
Aghajanova L, Skottman H, Stromberg AM, Inzunza J, Lahesmaa R, Hovatta O Expression of leukemia inhibitory factor and its receptors is increased during differentiation of human embryonic stem cells. Fertil. Steril., 2006-09-01;86(4):1193-209. 2006-09-01 [PMID: 16949591] (ICC, Human) ICC Human
Pan W, Cain C, Yu Y, Kastin AJ Receptor-mediated transport of LIF across blood-spinal cord barrier is upregulated after spinal cord injury. J. Neuroimmunol., 2006-03-06;174(1):119-25. 2006-03-06 [PMID: 16563523] (Neutralization, Mouse) Neutralization Mouse
Queen MM, Ryan RE, Holzer RG, Keller-Peck CR, Jorcyk CL Breast cancer cells stimulate neutrophils to produce oncostatin M: potential implications for tumor progression. Cancer Res., 2005-10-01;65(19):8896-904. 2005-10-01 [PMID: 16204061] (Neutralization, Human) Neutralization Human
He W, Gong K, Smith DK, Ip NY The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling. FEBS Lett., 2005-08-15;579(20):4317-23. 2005-08-15 [PMID: 16051226] (Western Blot, Human) Western Blot Human
Abir R, Fisch B, Jin S, Barnnet M, Freimann S, van den Hurk R, Feldberg D, Nitke S, Krissi H, Ao A Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries. Mol. Hum. Reprod., 2004-03-25;10(5):313-9. 2004-03-25 [PMID: 15044601] (IHC-Fr, Human) IHC-Fr Human
Malm C, Sjodin TL, Sjoberg B, Lenkei R, Renstrom P, Lundberg IE, Ekblom B Leukocytes, cytokines, growth factors and hormones in human skeletal muscle and blood after uphill or downhill running. J. Physiol. (Lond.), 2004-02-06;556(0):983-1000. 2004-02-06 [PMID: 14766942] (IHC-Fr, Human) IHC-Fr Human
Show All 12 Publications.

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Gene Symbol LIFR