LIF Antibody [Unconjugated]

Images

 
LIF was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA) at 10 µg/mL overnight at 4 °C. ...read more
Recombinant Human LIF (Catalog # 7734-LF) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-depend­ent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation ...read more
LIF was detected in immersion fixed paraffin-embedded sections of human lung array using Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA) at 25 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

LIF Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human LIF
Ser23-Phe202
Accession # P15018
Specificity
Detects human LIF in direct ELISAs. In direct ELISAs, less than 45% cross-reactivity with recombinant mouse LIF and less than
15% cross-reactivity with recombinant rat LIF is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LIF
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.04-0.08 ug/mL
Control
LIF Knockout HeLa Cell Lysate
Publications
Read Publications using
AF-250-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIF Antibody [Unconjugated]

  • CDF
  • D Factor
  • DIA
  • differentiation inhibitory activity
  • differentiation stimulating factor
  • Differentiation-stimulating factor
  • Emfilermin
  • HILDA
  • HILDAcholinergic differentiation factor
  • leukemia inhibitory factor (cholinergic differentiation factor)
  • leukemia inhibitory factor
  • LIF
  • Melanoma-derived LPL inhibitor
  • MLPLI

Background

LIF (Leukemia inhibitory factor; also  Differentiation-stimulating factor) is a 22 kDa (predicted) glycoprotein, member of  the leukemia inhibitory factor/interleukin-6 (LIF/IL-6) family of cytokines. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, it is produced by a variety of cells including T cells, monocytes, fibroblasts, osteoblasts and mast cells. LIF induces hematopoietic differentiation in normal and myeloid leukemia cells, neuronal cell differentiation and stimulation of acute-phase protein synthesis in hepatocytes. Human LIF acts through a receptor comprising a 190 kDa LIF-binding alpha -chain and a 130 kDa signal-transducing beta -chain (gp130), which is shared with the other members of the IL-6 family. Human and mouse LIF exhibit 78% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LIF Antibody (AF-250-NA)(13)

We have publications tested in 2 confirmed species: Human, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 5 applications: ICC, IHC, IHC-P, Neutralization, Western Blot.


Filter By Application
ICC
(3)
IHC
(1)
IHC-P
(5)
Neutralization
(3)
Western Blot
(1)
All Applications
Filter By Species
Human
(12)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF-250-NA Applications Species
M Kessler, K Hoffmann, K Fritsche, V Brinkmann, HJ Mollenkopf, O Thieck, AR Teixeira d, EI Braicu, J Sehouli, M Mangler, H Berger, TF Meyer Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation Nat Commun, 2019;10(1):1194. 2019 [PMID: 30886143] (Neutralization, Human) Neutralization Human
JE Yoon, Y Kim, S Kwon, M Kim, YH Kim, JH Kim, TJ Park, HY Kang Senescent fibroblasts drive ageing pigmentation: ?A potential therapeutic target for senile lentigo Theranostics, 2018;8(17):4620-4632. 2018 [PMID: 30279727] (IHC-P, Human) IHC-P Human
F Wu, X Chen, Y Liu, B Liang, H Xu, TC Li, CC Wang Decreased MUC1 in endometrium is an independent receptivity marker in recurrent implantation failure during implantation window Reprod. Biol. Endocrinol., 2018;16(1):60. 2018 [PMID: 29929546] (IHC, Human) IHC Human
C Ueshima, TR Kataoka, M Hirata, A Sugimoto, Y Iemura, S Minamiguch, T Nomura, H Haga Possible involvement of human mast cells in the establishment of pregnancy via KIR2DL4 Am. J. Pathol., 2018;0(0):. 2018 [PMID: 29577936] (Neutralization, Human) Neutralization Human
Xiao Y, Sun X, Yang X, Zhang J, Xue Q, Cai B, Zhou Y Leukemia inhibitory factor is dysregulated in the endometrium and uterine flushing fluid of patients with adenomyosis during implantation window. Fertil. Steril., 2010;94(1):85-9. 2010 [PMID: 19361790] (IHC-P, Human) IHC-P Human
Aghajanova L, Altmae S, Bjuresten K, Hovatta O, Landgren BM, Stavreus-Evers A Disturbances in the LIF pathway in the endometrium among women with unexplained infertility. Fertil. Steril., 2009;91(6):2602-10. 2009 [PMID: 18684446] (IHC-P, Human) IHC-P Human
Meng CX, Andersson KL, Bentin-Ley U, Gemzell-Danielsson K, Lalitkumar PG Effect of levonorgestrel and mifepristone on endometrial receptivity markers in a three-dimensional human endometrial cell culture model. Fertil. Steril., 2009;91(1):256-64. 2009 [PMID: 18206148] (ICC, Human) ICC Human
Kamohara H, Ogawa M, Ishiko T, Sakamoto K, Baba H Leukemia inhibitory factor functions as a growth factor in pancreas carcinoma cells: Involvement of regulation of LIF and its receptor expression. Int. J. Oncol., 2007;30(4):977-83. 2007 [PMID: 17332938] (Neutralization, Human) Neutralization Human
Aghajanova L, Skottman H, Stromberg AM, Inzunza J, Lahesmaa R, Hovatta O Expression of leukemia inhibitory factor and its receptors is increased during differentiation of human embryonic stem cells. Fertil. Steril., 2006;86(4):1193-209. 2006 [PMID: 16949591] (ICC, Human) ICC Human
He W, Gong K, Smith DK, Ip NY The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling. FEBS Lett., 2005;579(20):4317-23. 2005 [PMID: 16051226] (Western Blot, Human) Western Blot Human
Show All 13 Publications.

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF250

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Blogs on LIF.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

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Bioinformatics

Gene Symbol LIF
Uniprot