Lgr5/GPR49 Antibody (CL13567) - BSA Free

Staining of human skeletal muscle shows moderate membranous positivity in myocytes.

Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Western blot analysis in human cell line SH-SY5Y.

Staining of human ovarian cancer (dysgerminoma) shows strong membranous and cytoplasmic positivity in tumor cells.

Staining of human cerebral cortex shows moderate membranous positivity in a subset of neurons.

Staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.

Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.

Staining of human ovarian cancer (high grade serous ovarian carcinoma) shows strong cytoplasmic positivity in tumor cells.

Analysis in human fallopian tube and tonsil tissues using NBP3-24570 antibody. Corresponding LGR5 RNA-seq data are presented for the same tissues.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: CL13567
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Lgr5/GPR49 Antibody (CL13567) - BSA Free Summary

• Description: Novus Biologicals Mouse Lgr5/GPR49 Antibody (CL13567) - BSA Free (NBP3-24570) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a synthetic peptide derived from O75473, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (91%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LGR5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1ug/ml
• Application Notes: For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Reactivity Notes

Rat (87%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Lgr5/GPR49 Antibody (CL13567) - BSA Free

• FEX
• G protein-coupled receptor 49
• GPR49
• GPR49G-protein coupled receptor HG38
• GPR67
• G-protein coupled receptor 49
• G-protein coupled receptor 67
• GRP49
• HG38
• leucine-rich repeat containing G protein-coupled receptor 5
• leucine-rich repeat-containing G protein-coupled receptor 5
• leucine-rich repeat-containing G-protein coupled receptor 5
• Lgr5
• MGC117008
• orphan G protein-coupled receptor HG38

Background

Leucine-rich repeat-containing LGR5, also known as GPR49, is an Orphan-A G-protein coupled receptor with an unknown ligand. GPR49 is a stem cell marker of the intestinal epithelium and the hair follicule, and is often expressed in the brain, skeletal muscle, placenta, and spinal cord. Because LGR5 is typically found in mouse hair follicles it may potentially be used to regrow hair in mice. GPR49/LGR5 antibodies are useful tools for research on hair loss and hair follicule development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LGR5