Lgr5/GPR49 Antibody (CL13567) - Azide and BSA Free

Analysis in human fallopian tube and tonsil tissues using NBP3-43844 antibody. Corresponding Lgr5/GPR49 RNA-seq data are presented for the same tissues.

Staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.

Staining of human cerebral cortex shows moderate membranous positivity in a subset of neurons.

Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.

Staining of human ovarian cancer (dysgerminoma) shows strong membranous and cytoplasmic positivity in tumor cells.

Staining of human skeletal muscle shows moderate membranous positivity in myocytes.

Staining of human ovarian cancer (high grade serous ovarian carcinoma) shows strong cytoplasmic positivity in tumor cells.

Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Analysis in human cell line SH-SY5Y.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL13567
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Lgr5/GPR49 Antibody (CL13567) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a synthetic peptide of O75473, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LGR5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Lgr5/GPR49 Antibody (CL13567) - Azide and BSA Free

• FEX
• G protein-coupled receptor 49
• GPR49
• GPR49G-protein coupled receptor HG38
• GPR67
• G-protein coupled receptor 49
• G-protein coupled receptor 67
• GRP49
• HG38
• leucine-rich repeat containing G protein-coupled receptor 5
• leucine-rich repeat-containing G protein-coupled receptor 5
• leucine-rich repeat-containing G-protein coupled receptor 5
• Lgr5
• MGC117008
• orphan G protein-coupled receptor HG38

Background

Leucine-rich repeat-containing LGR5, also known as GPR49, is an Orphan-A G-protein coupled receptor with an unknown ligand. GPR49 is a stem cell marker of the intestinal epithelium and the hair follicule, and is often expressed in the brain, skeletal muscle, placenta, and spinal cord. Because LGR5 is typically found in mouse hair follicles it may potentially be used to regrow hair in mice. GPR49/LGR5 antibodies are useful tools for research on hair loss and hair follicule development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LGR5