Lgr5/GPR49 Antibody (4H8) - BSA Free

Western Blot: Lgr5/GPR49 Antibody (4H8) [NBP3-15237] - Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue. All lanes: Lgr5/GPR49 antibody at 1:1500. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 100, 98, 93 KDa. Observed band size: 100 KDa.

Flow Cytometry: Lgr5/GPR49 Antibody (4H8) [NBP3-15237] - Overlay histogram showing HepG2 cells stained with Lgr5/GPR49 antibody (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Flow
• Clone: 4H8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Lgr5/GPR49 Antibody (4H8) - BSA Free Summary

• Description: Novus Biologicals Rabbit Lgr5/GPR49 Antibody (4H8) - BSA Free (NBP3-15237) is a recombinant monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from human LGR5/GPR49. UniProt ID (O75473).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LGR5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Lgr5/GPR49 Antibody (4H8) - BSA Free

• FEX
• G protein-coupled receptor 49
• GPR49
• GPR49G-protein coupled receptor HG38
• GPR67
• G-protein coupled receptor 49
• G-protein coupled receptor 67
• GRP49
• HG38
• leucine-rich repeat containing G protein-coupled receptor 5
• leucine-rich repeat-containing G protein-coupled receptor 5
• leucine-rich repeat-containing G-protein coupled receptor 5
• Lgr5
• MGC117008
• orphan G protein-coupled receptor HG38

Background

Leucine-rich repeat-containing LGR5, also known as GPR49, is an Orphan-A G-protein coupled receptor with an unknown ligand. GPR49 is a stem cell marker of the intestinal epithelium and the hair follicule, and is often expressed in the brain, skeletal muscle, placenta, and spinal cord. Because LGR5 is typically found in mouse hair follicles it may potentially be used to regrow hair in mice. GPR49/LGR5 antibodies are useful tools for research on hair loss and hair follicule development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LGR5