Leptin R Antibody [Biotin]

HEK293 human cell line transfected with Mouse Leptin R and EGFP was stained with (A) Goat Anti-Mouse Leptin R Biotinylated Polyclonal Antibody (Catalog # BAF497) or (B) control antibody (BAF108), followed by Allophycocyanin-conjugated Streptavidin (F0050). Staining was performed using our Staining Membrane-associated Proteins protocol.

The MC in the mouse LN is a unique structure comprising MCRCs. (A) LepR is highly expressed in the medulla. A section of a brachial LN from a wild type B6 mouse was stained with the indicated markers. The dotted lines indicate the boundary between the DCP and medulla. (B) FSCs in the MC showed the highest expression of LepR. The inset shows a higher magnification view of a MC. (C) Average fluorescent intensity profiles of B220, desmin, and LepR along the cortex–medulla axis. Note that LepR expression is prominent in the medulla, and shows a similar pattern to LYVE-1 in Figure 3D, indicating preferential expression in the medulla. (D) Plasma cells preferentially accumulate in the MCs. Shown is a wild type B6 mouse LN. (E) Plasma cells make close contacts with MCRCs. Shown is a Cxcl12-EGFP mouse LN. (F,G) Quantitative analysis of plasma cell localization in LN subcompartments (bars: mean; asterisks: significantly different compared to the follicles, p < 0.0001). (F) A representative image of plasma cells. The dotted lines indicate the boundary between the DCP and medulla. (G) Proportions of plasma cells in each subcompartment compared to the total number in the LN were plotted (open circles; bars: mean; *p < 0.0001). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30333825), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, Flow, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Leptin R Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Leptin R
  Ala20-Gly839
  Accession # Q3US58
• Specificity: Detects mouse Leptin R in ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LEPR
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse Leptin R Antibody (Catalog # MAB497)
  ELISA Detection: Mouse Leptin R Biotinylated Antibody (Catalog # BAF497)
  Standard: Recombinant Mouse Leptin R Fc Chimera (Catalog # 497-LR)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Leptin R Antibody [Biotin]

• B219
• CD295 antigen
• CD295
• DB
• DKFZp686B1731
• huB219
• LEPR
• LEP-R
• Leptin R
• leptin receptor
• LeptinR
• OB R
• OB receptor
• OB-R
• OBRCD295

Background

Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-R_L) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-R_s) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript lacking a transmembrane domain and potentially encoding a soluble form of the receptor has also been described. Circulating soluble OB-R, complexed to leptin, has been detected in mouse serum. Serum soluble OB-R levels have been shown to increase during pregnancy. OB-R_L transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-R_s transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney and primitive hematopoietic cell populations. OB-R has recently been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype.

Mouse OB-R long form encodes a 1162 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 817 aa residue extracellular domain, a 21 aa residue transmembrane domain, and a 302 aa residue cytoplasmic domain. The extracellular domain of OB-R contains two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant murine soluble OB-R has been shown to bind leptin with high affinity and is a potent leptin antagonist.

1. Tartaglia, L.A. et al. (1995) Cell 83:1263.
2. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
3. Lee, J.I. and J.M. Friedman (1996) Nature 379:632.
4. Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.
5. Gavrilova, O. et al. (1997) J. Biol. Chem. 272:30546.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Leptin R Antibody (BAF497)(42)

Publications using BAF497

• Li, K;Hu, S;Li, H;Lin, W;Zhao, D;Xu, Z;Pan, C;Wang, H;Li, D;Liu, J;Chen, H; Regenerating aged bone marrow via a nitric oxide nanopump Nature communications 2025-07-01 [PMID: 40593809] (Bioassay, In vivo assay, Mouse)
• Krepuska, M;Mayer, B;Vitale-Cross, L;Myneni, VD;Boyajian, MK;Németh, K;Szalayova, I;Cho, T;Mc Clain-Caldwell, I;Gingerich, AD;Han, H;Westerman, M;Rada, B;Mezey, É; Bone marrow stromal cell-derived hepcidin has antimicrobial and immunomodulatory activities Scientific reports 2024-02-17 [PMID: 38368463] (Immunohistochemistry, Human)
• Yan Lin, Quan Gu, Shihong Lu, Zengkai Pan, Zining Yang, Yapu Li et al. Ruxolitinib improves hematopoietic regeneration by restoring mesenchymal stromal cell function in acute graft-versus-host disease Journal of Clinical Investigation 8/1/2023 [PMID: 37338986]
• Yuriko Sorimachi, Hiroshi Kobayashi, Yusuke Shiozawa, Shuhei Koide, Ryuichiro Nakato, Yukiko Shimizu et al. Mesenchymal loss of p53 alters stem cell capacity and models human soft tissue sarcoma traits Stem Cell Reports 2023-05-09 [PMID: 37059101] (Flow Cytometry, Mouse, Transgenic Mouse)
• Yuriko Sorimachi, Hiroshi Kobayashi, Yusuke Shiozawa, Shuhei Koide, Ryuichiro Nakato, Yukiko Shimizu et al. Mesenchymal loss of p53 alters stem cell capacity and models human soft tissue sarcoma traits Stem Cell Reports 5/9/2023 [PMID: 37059101]
• P Ramalingam, MC Gutkin, MG Poulos, T Tillery, C Doughty, A Winiarski, AG Freire, S Rafii, D Redmond, JM Butler Restoring bone marrow niche function rejuvenates aged hematopoietic stem cells by reactivating the DNA Damage Response Nature Communications, 2023-04-10;14(1):2018. 2023-04-10 [PMID: 37037837] (ICC, MACS, Mouse)
• Hotaka Kawai, May Wathone Oo, Kiyofumi Takabatake, Ikue Tosa, Yamin Soe, Htoo Shwe Eain et al. Enzyme‐Cleaved Bone Marrow Transplantation Improves the Engraftment of Bone Marrow Mesenchymal Stem Cells JBMR Plus 2023-03-01 [PMID: 36936364] (Flow Cytometry, Mouse)
• Hotaka Kawai, May Wathone Oo, Kiyofumi Takabatake, Ikue Tosa, Yamin Soe, Htoo Shwe Eain et al. Enzyme‐Cleaved Bone Marrow Transplantation Improves the Engraftment of Bone Marrow Mesenchymal Stem Cells JBMR Plus 3/1/2023 [PMID: 36936364]
• Martijn P. T. Ernst, Eline Pronk, Claire van Dijk, Paulina M. H. van Strien, Tim V. D. van Tienhoven, Michiel J. W. Wevers et al. Hematopoietic Cell Autonomous Disruption of Hematopoiesis in a Germline Loss-of-function Mouse Model of RUNX1-FPD HemaSphere 2/1/2023 [PMID: 36741355]
• Carl A. Mitchell, Evgenia V. Verovskaya, Fernando J. Calero-Nieto, Oakley C. Olson, James W. Swann, Xiaonan Wang et al. Stromal niche inflammation mediated by IL-1 signalling is a targetable driver of haematopoietic ageing Nature Cell Biology 2023-01-17 [PMID: 36650381] (Flow Cytometry, Mouse)
• Carl A. Mitchell, Evgenia V. Verovskaya, Fernando J. Calero-Nieto, Oakley C. Olson, James W. Swann, Xiaonan Wang et al. Stromal niche inflammation mediated by IL-1 signalling is a targetable driver of haematopoietic ageing Nature Cell Biology 1/17/2023 [PMID: 36650381]
• EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair Cell Stem Cell, 2022-10-21;29(11):1547-1561.e6. 2022-10-21 [PMID: 36272401] (IHC, Mouse)
• Siddaraju V. Boregowda, Manjunatha K. Nanjappa, Cori N. Booker, Jacqueline Strivelli, Valentina M. Supper, Paul S. Cooke et al. Pharmacological Inhibition of Inositol Hexakisphosphate Kinase 1 Protects Mice against Obesity-Induced Bone Loss Biology (Basel) 8/24/2022 [PMID: 36138736]
• MJ Schloss, M Hulsmans, D Rohde, IH Lee, N Severe, BH Foy, FE Pulous, S Zhang, KD Kokkaliari, V Frodermann, G Courties, C Yang, Y Iwamoto, AS Knudsen, CS McAlpine, M Yamazoe, SP Schmidt, GR Wojtkiewic, GS Masson, K Gustafsson, D Capen, D Brown, JM Higgins, DT Scadden, P Libby, FK Swirski, K Naxerova, M Nahrendorf B lymphocyte-derived acetylcholine limits steady-state and emergency hematopoiesis Nature Immunology, 2022-03-28;23(4):605-618. 2022-03-28 [PMID: 35352063] (ICC, Mouse)
• YS Lee, TY Kim, Y Kim, S Kim, SH Lee, SU Seo, BO Zhou, O Eunju, KS Kim, MN Kweon Microbiota-derived lactate promotes hematopoiesis and erythropoiesis by inducing stem cell factor production from leptin receptor+ niche cells Experimental & Molecular Medicine, 2021-09-09;0(0):. 2021-09-09 [PMID: 34497346] (Flow Cytometry, ICC, IHC, Mouse)
• Ambrosi TH, Sinha R, Steininger HM et al. Distinct skeletal stem cell types orchestrate long bone skeletogenesis eLife 7/19/2021 [PMID: 34280086]
• B Shen, A Tasdogan, JM Ubellacker, J Zhang, ED Nosyreva, L Du, MM Murphy, S Hu, Y Yi, N Kara, X Liu, S Guela, Y Jia, V Ramesh, C Embree, EC Mitchell, YC Zhao, LA Ju, Z Hu, GM Crane, Z Zhao, R Syeda, SJ Morrison A mechanosensitive peri-arteriolar niche for osteogenesis and lymphopoiesis Nature, 2021-02-24;0(0):. 2021-02-24 [PMID: 33627868] (IHC, Mouse)
• M Mohrin, J Liu, J Zavala-Sol, S Bhargava, J Maxwell Tr, A Brito, D Hu, D Brooks, G Koukos, L Alabdulaal, JS Paw, K Hake, G Kolumam, ML Bouxsein, R Baron, Y Kutskova, A Freund Inhibition of longevity regulator PAPP-A modulates tissue homeostasis via restraint of mesenchymal stromal cells Aging Cell, 2021-02-09;0(0):e13313. 2021-02-09 [PMID: 33561324] (Flow Cytometry, Mouse)
• T Trinh, J Ropa, A Aljoufi, S Cooper, A Sinn, EF Srour, HE Broxmeyer Leptin receptor, a surface marker for a subset of highly engrafting long-term functional hematopoietic stem cells Leukemia, 2020-11-07;0(0):. 2020-11-07 [PMID: 33159180] (ICC, Mouse)
• Shuji Nosho, Ikue Tosa, Mitsuaki Ono, Emilio Satoshi Hara, Kei Ishibashi, Akihiro Mikai et al. Distinct Osteogenic Potentials of BMP-2 and FGF-2 in Extramedullary and Medullary Microenvironments International Journal of Molecular Sciences 10/27/2020 [PMID: 33120952]
• Van Gastel N, Spinelli Jb, Sharda A Et Al. Induction of a Timed Metabolic Collapse to Overcome Cancer Chemoresistance Cell Metab. 9/1/2020 [PMID: 32763164]
• DD He, XT Tang, W Dong, G Cui, G Peng, X Yin, Y Chen, N Jing, BO Zhou C-KIT Expression Distinguishes Fetal from Postnatal Skeletal Progenitors Stem Cell Reports, 2020-03-26;0(0):. 2020-03-26 [PMID: 32220331] (Flow Cytometry, Mouse)
• B Gao, J Yin, X Xu, J Fan, D Wang, C Zheng, W Lu, P Cheng, J Sun, D Wang, L Li, BO Zhou, L Yang, Z Luo Leptin receptor-expressing cells represent a distinct subpopulation of notochord-derived cells and are essential for disc homoeostasis J Orthop Translat, 2019-12-24;21(0):91-99. 2019-12-24 [PMID: 32110508] (IHC, Mouse)
• Dayana B. Rivadeneira, Kristin DePeaux, Yiyang Wang, Aditi Kulkarni, Tracy Tabib, Ashley V. Menk et al. Oncolytic Viruses Engineered to Enforce Leptin Expression Reprogram Tumor-Infiltrating T Cell Metabolism and Promote Tumor Clearance Immunity 9/1/2019 [PMID: 31471106]
• N Severe, NM Karabacak, K Gustafsson, N Baryawno, G Courties, Y Kfoury, KD Kokkaliari, C Rhee, D Lee, EW Scadden, JE Garcia-Rob, T Brouse, M Nahrendorf, M Toner, DT Scadden Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping Cell Stem Cell, 2019-07-03;0(0):. 7/3/2019 [PMID: 31279774]
• W Zou, N Rohatgi, JR Brestoff, Y Zhang, EL Scheller, CS Craft, MD Brodt, N Migotsky, MJ Silva, CA Harris, SL Teitelbaum Congenital lipodystrophy induces severe osteosclerosis PLoS Genet., 2019-06-24;15(6):e1008244. 2019-06-24 [PMID: 31233501] (Flow Cytometry, Mouse)
• J Song, J Li, F Yang, G Ning, L Zhen, L Wu, Y Zheng, Q Zhang, D Lin, C Xie, L Peng Nicotinamide mononucleotide promotes osteogenesis and reduces adipogenesis by regulating mesenchymal stromal cells via the SIRT1 pathway in aged bone marrow Cell Death Dis, 2019-04-18;10(5):336. 2019-04-18 [PMID: 31000692] (Flow Cytometry, Mouse)
• B Gao, R Deng, Y Chai, H Chen, B Hu, X Wang, S Zhu, Y Cao, S Ni, M Wan, L Yang, Z Luo, X Cao Macrophage-lineage TRAP+ cells recruit periosteum-derived cells for periosteal osteogenesis and regeneration J. Clin. Invest., 2019-04-04;129(6):2578-2594. 2019-04-04 [PMID: 30946695] (Flow Cytometry, IHC, Mouse)
• M Balzano, M De Grandis, TP Vu Manh, L Chasson, F Bardin, A Farina, A Sergé, G Bidaut, P Charbord, L Hérault, AL Bailly, A Cartier-Mi, A Boned, M Dalod, E Duprez, P Genever, M Coles, M Bajenoff, L Xerri, M Aurrand-Li, C Schiff, SJC Mancini Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche Cell Rep, 2019-03-19;26(12):3257-3271.e8. 2019-03-19 [PMID: 30893599] (IHC, Mouse)
• M Zhao, F Tao, A Venkatrama, Z Li, SE Smith, J Unruh, S Chen, C Ward, P Qian, JM Perry, H Marshall, J Wang, XC He, L Li N-Cadherin-Expressing Bone and Marrow Stromal Progenitor Cells Maintain Reserve Hematopoietic Stem Cells Cell Rep, 2019-01-15;26(3):652-669.e6. 2019-01-15 [PMID: 30650358] (Flow Cytometry, Mouse)
• C Lo Sicco, D Reverberi, F Villa, U Pfeffer, R Quarto, R Cancedda, R Tasso Circulating healing (CH) cells expressing BST2 are functionally activated by the injury-regulated systemic factor HGFA Stem Cell Res Ther, 2018-11-08;9(1):300. 2018-11-08 [PMID: 30409222] (Flow Cytometry, Mouse)
• Li CJ, Xiao Y, Yang M et al. Long noncoding RNA Bmncr regulates mesenchymal stem cell fate during skeletal aging. J. Clin. Invest. 10/22/2018 [PMID: 30352426]
• Arata Takeuchi, Madoka Ozawa, Yasuhiro Kanda, Mina Kozai, Izumi Ohigashi, Yoichi Kurosawa et al. A Distinct Subset of Fibroblastic Stromal Cells Constitutes the Cortex-Medulla Boundary Subcompartment of the Lymph Node Frontiers in Immunology 10/2/2018 [PMID: 30333825]
• Ke, X, Do, D C Et al. Ras homolog family member A/Rho-associated protein kinase 1 signaling modulates lineage commitment of mesenchymal stem cells in asthmatic patients through lymphoid enhancer-binding factor 1. J Allergy Clin Immunol 4/1/2019 [PMID: 30194990]
• B Gao, X Lin, H Jing, J Fan, C Ji, Q Jie, C Zheng, D Wang, X Xu, Y Hu, W Lu, Z Luo, L Yang Local delivery of tetramethylpyrazine eliminates the senescent phenotype of bone marrow mesenchymal stromal cells and creates an anti-inflammatory and angiogenic environment in aging mice Aging Cell, 2018-02-28;0(0):. 2018-02-28 [PMID: 29488314] (Flow Cytometry, Mouse)
• Rui Yue, Bo Shen, Sean J Morrison Clec11a/osteolectin is an osteogenic growth factor that promotes the maintenance of the adult skeleton eLife 12/13/2016 [PMID: 27976999]
• Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation Nat. Med, 2016-12-12;0(0):. 2016-12-12 [PMID: 27941793] (Flow Cytometry, Mouse)
• Joshua N Farr Identification of Senescent Cells in the Bone Microenvironment J Bone Miner Res, 2016-10-24;0(0):. 2016-10-24 [PMID: 27341653] (Cell Selection, Mouse)
• Douaa Dhahri Fibrinolytic factor-mediated crosstalk with endothelial cells expands murine bone marrow mesenchymal stromal cells Blood, 2016-06-09;0(0):. 6/9/2016 [PMID: 27283026]
• Garcin G, Paul F, Staufenbiel M, Bordat Y, Van der Heyden J, Wilmes S, Cartron G, Apparailly F, De Koker S, Piehler J, Tavernier J, Uze G High efficiency cell-specific targeting of cytokine activity. Nat Commun, 2014-01-01;5(0):3016. 2014-01-01 [PMID: 24398568] (Flow Cytometry, Mouse)
• Marie CS, Verkerke HP, Paul SN Leptin protects host cells from Entamoeba histolytica cytotoxicity by a STAT3-dependent mechanism. Infect. Immun., 2012-02-13;80(5):1934-43. 2012-02-13 [PMID: 22331430] (Western Blot, Mouse)
• Gruver AL, Ventevogel MS, Sempowski GD Leptin receptor is expressed in thymus medulla and leptin protects against thymic remodeling during endotoxemia-induced thymus involution. J. Endocrinol., 2009-07-08;203(1):75-85. 2009-07-08 [PMID: 19587263] (ICC, IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: LEPR
• Uniprot:
  • Q3US58()