Human AgRP Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human AgRP Quantikine ELISA Kit Summary

• Background: The Quantikine Human AgRP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human AgRP in cell culture supernates, plasma, and urine. It contains Sf 21-expressed recombinant human AgRP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human AgRP showed linear curves that were parallel to the standard curves obtained usin...g the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human AgRP.
  Show More
• Specificity: Natural and recombinant human AgRP
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: AGRP

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human AgRP Quantikine ELISA Kit

• agouti related protein homolog (mouse)
• agouti-related protein
• AgRP
• Agrt
• AGRTMGC118963
• ART
• ARTagouti (mouse) related protein
• ASIP2

Background

Agouti-related Protein (AgRP), also known as the protein product of the Agouti-related Transcript (ART), is a neuropeptide and a functional antagonist of alpha-MSH on Melanocortin (MC)-3 and MC-4 receptors, both of which are implicated in obesity. AgRP shares sequence and structural similarity with the Agouti protein and has similar expression patterns in both mouse and human.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for AgRP/ART (DAGR00)(3)

Publications using DAGR00

• NG Nelson, L Wu, MT Maier, D Lam, R Cheang, D Alba, A Huang, DA Neumann, T Hill, E Vagena, GS Barsh, MW Medina, RM Krauss, SK Koliwad, AW Xu A gene-diet interaction controlling relative intake of dietary carbohydrates and fats Molecular Metabolism, 2022-01-19;0(0):101442. 2022-01-19 [PMID: 35051651] (Human)
• Xiao E, Kim AJ, Dutia R Effects of estradiol on cerebrospinal fluid levels of agouti-related protein in ovariectomized rhesus monkeys. Endocrinology, 2010-01-07;151(3):1002-9. 2010-01-07 [PMID: 20056830] (Primate - Macaca mulatta (Rhesus Macaque))
• Moriya J, Takimoto Y, Yoshiuchi K Plasma agouti-related protein levels in women with anorexia nervosa. Psychoneuroendocrinology, 2006-08-14;31(9):1057-61. 2006-08-14 [PMID: 16904835] (Human)

FAQs for AgRP/ART (DAGR00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: AGRP