Leptin/OB Antibody [Unconjugated]

398-LP), Recombinant Mouse Leptin/OB (Catalog # 498-OB) and Recombinant Rat Leptin/OB (Catalog # 598-LP). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Leptin/OB at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." > 398-LP), Recombinant Mouse Leptin/OB (Catalog # 498-OB) and Recombinant Rat Leptin/OB (Catalog # 598-LP). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Leptin/OB at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." title="Western blot shows 100 ng of Recombinant Human Leptin/OB (Catalog # 398-LP), Recombinant Mouse Leptin/OB (Catalog # 498-OB) and Recombinant Rat Leptin/OB (Catalog # 598-LP). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Leptin/OB at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." />

Western blot shows 100 ng of Recombinant Human Leptin/OB (Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="Leptin/OB was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF398) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

Leptin/OB was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF398) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

398-LP) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Leptin/OB (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF398). The ND₅₀ is typically 0.5-3.0 µg/mL." > 398-LP) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Leptin/OB (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF398). The ND₅₀ is typically 0.5-3.0 µg/mL." title="Recombinant Human Leptin/OB (Catalog # 398-LP) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Leptin/OB (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF398). The ND₅₀ is typically 0.5-3.0 µg/mL." />

Recombinant Human Leptin/OB (Catalog # <a class=

Effect of the body mass index on epithelial polarity and proliferation in normal breast tissues. A Leptin and adiponectin levels as a function of BMI in serum from women who donated breast tissue specimens to the Komen Tissue Bank. Spearman correlation coefficients (r) are indicated with corresponding P values. b Cross-sectional size of adipocytes in breast tissue sections from KTB donors. r, Pearson’s correlation coefficient. c ZO-1 radial distribution and continuity in breast tissue sections as a function of BMI. Microscopy images from two different donors are shown for illustration. Cell nuclei were stained with DAPI. Statistical comparisons (normal weight vs. obesity) with Mann Whitney test. d Relative proportion of adipose tissue as a function of BMI. r, Pearson’s coefficient. e ZO-1 score (average of continuity and radial distribution) as a function of the adipose content of the tissues. r, Pearson’s coefficient. f Quantification of epithelial structures (ducts, lobules) with ≥1 Ki67-positive cells according to BMI categories. Representative immunostaining images are shown, with arrowheads pointing to Ki67-positive nuclei. Antibodies against NuMA were used as staining control. *P < 0.05 (ANOVA and Fischer’s LSD test). g Average number of epithelial cell layers in breast epithelia from different BMI categories, assessed on H&E images. *P < 0.05 (Kruskal–Wallis and Dunn’s test). Averages are shown on bar graphs. Scale bars: 50 µm. N normal weight, OW overweight, OB obese. Each symbol on the graphs represents a KTB donors. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37160903), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of the body mass index on epithelial polarity and proliferation in normal breast tissues. A Leptin and adiponectin levels as a function of BMI in serum from women who donated breast tissue specimens to the Komen Tissue Bank. Spearman correlation coefficients (r) are indicated with corresponding P values. b Cross-sectional size of adipocytes in breast tissue sections from KTB donors. r, Pearson’s correlation coefficient. c ZO-1 radial distribution and continuity in breast tissue sections as a function of BMI. Microscopy images from two different donors are shown for illustration. Cell nuclei were stained with DAPI. Statistical comparisons (normal weight vs. obesity) with Mann Whitney test. d Relative proportion of adipose tissue as a function of BMI. r, Pearson’s coefficient. e ZO-1 score (average of continuity and radial distribution) as a function of the adipose content of the tissues. r, Pearson’s coefficient. f Quantification of epithelial structures (ducts, lobules) with ≥1 Ki67-positive cells according to BMI categories. Representative immunostaining images are shown, with arrowheads pointing to Ki67-positive nuclei. Antibodies against NuMA were used as staining control. *P < 0.05 (ANOVA and Fischer’s LSD test). g Average number of epithelial cell layers in breast epithelia from different BMI categories, assessed on H&E images. *P < 0.05 (Kruskal–Wallis and Dunn’s test). Averages are shown on bar graphs. Scale bars: 50 µm. N normal weight, OW overweight, OB obese. Each symbol on the graphs represents a KTB donors. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37160903), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of the body mass index on epithelial polarity and proliferation in normal breast tissues. A Leptin and adiponectin levels as a function of BMI in serum from women who donated breast tissue specimens to the Komen Tissue Bank. Spearman correlation coefficients (r) are indicated with corresponding P values. b Cross-sectional size of adipocytes in breast tissue sections from KTB donors. r, Pearson’s correlation coefficient. c ZO-1 radial distribution and continuity in breast tissue sections as a function of BMI. Microscopy images from two different donors are shown for illustration. Cell nuclei were stained with DAPI. Statistical comparisons (normal weight vs. obesity) with Mann Whitney test. d Relative proportion of adipose tissue as a function of BMI. r, Pearson’s coefficient. e ZO-1 score (average of continuity and radial distribution) as a function of the adipose content of the tissues. r, Pearson’s coefficient. f Quantification of epithelial structures (ducts, lobules) with ≥1 Ki67-positive cells according to BMI categories. Representative immunostaining images are shown, with arrowheads pointing to Ki67-positive nuclei. Antibodies against NuMA were used as staining control. *P < 0.05 (ANOVA and Fischer’s LSD test). g Average number of epithelial cell layers in breast epithelia from different BMI categories, assessed on H&E images. *P < 0.05 (Kruskal–Wallis and Dunn’s test). Averages are shown on bar graphs. Scale bars: 50 µm. N normal weight, OW overweight, OB obese. Each symbol on the graphs represents a KTB donors. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37160903), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of the body mass index on epithelial polarity and proliferation in normal breast tissues. A Leptin and adiponectin levels as a function of BMI in serum from women who donated breast tissue specimens to the Komen Tissue Bank. Spearman correlation coefficients (r) are indicated with corresponding P values. b Cross-sectional size of adipocytes in breast tissue sections from KTB donors. r, Pearson’s correlation coefficient. c ZO-1 radial distribution and continuity in breast tissue sections as a function of BMI. Microscopy images from two different donors are shown for illustration. Cell nuclei were stained with DAPI. Statistical comparisons (normal weight vs. obesity) with Mann Whitney test. d Relative proportion of adipose tissue as a function of BMI. r, Pearson’s coefficient. e ZO-1 score (average of continuity and radial distribution) as a function of the adipose content of the tissues. r, Pearson’s coefficient. f Quantification of epithelial structures (ducts, lobules) with ≥1 Ki67-positive cells according to BMI categories. Representative immunostaining images are shown, with arrowheads pointing to Ki67-positive nuclei. Antibodies against NuMA were used as staining control. *P < 0.05 (ANOVA and Fischer’s LSD test). g Average number of epithelial cell layers in breast epithelia from different BMI categories, assessed on H&E images. *P < 0.05 (Kruskal–Wallis and Dunn’s test). Averages are shown on bar graphs. Scale bars: 50 µm. N normal weight, OW overweight, OB obese. Each symbol on the graphs represents a KTB donors. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37160903), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Leptin/OB Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Leptin/OB
  Val22-Cys167
  Accession # Q6NT58
• Specificity: Detects human Leptin/OB in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 25% cross-reactivity with recombinant mouse Leptin/OB is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LEP
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.5-3.0 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Leptin/OB Antibody [Unconjugated]

• FLJ94114
• LEP
• leptin (murine obesity homolog)
• leptin (obesity homolog, mouse)
• Leptin
• OB
• Obese protein
• obese, mouse, homolog of
• Obesity factor
• OBOBS

Background

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the sythesis of Leptin have been found to be obese, diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Human Leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1 - 3). The roles of leptin in body weight regulation, hematopoiesis and reproduction continue to be investigated.

1. Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
2. Tartaglia, L.A. et al. (1995) Cell 83:1263.
3. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Leptin/OB Antibody (AF398)(6)

Publications using AF398

• Julia Holmes, Mohamed Gaber, Mónica Z. Jenks, Adam Wilson, Tucker Loy, Cassandra Lepetit et al. Reversion of breast epithelial polarity alterations caused by obesity npj Breast Cancer 5/9/2023 [PMID: 37160903]
• Christine Frithioff‐Bøjsøe, Morten A.V. Lund, Ulrik Lausten‐Thomsen, Paula L. Hedley, Oluf Pedersen, Michael Christiansen et al. Leptin, adiponectin, and their ratio as markers of insulin resistance and cardiometabolic risk in childhood obesity Pediatric Diabetes 3/1/2020 [PMID: 31845423]
• UV-induced inhibition of adipokine production in subcutaneous fat aggravates dermal matrix degradation in human skin Sci Rep, 2016-05-10;6(0):25616. 2016-05-10 [PMID: 27161953] (IHC-Fr, Human)
• Mark Duquette, Peter M. Sadow, Amjad Husain, Jennifer N. Sims, Zeus A. Antonello, Andrew H. Fischer et al. Metastasis-associated MCL1 and P16 copy number alterations dictate resistance to vemurafenib in a BRAFV600E patient-derived papillary thyroid carcinoma preclinical model Oncotarget 11/30/2015 [PMID: 26636651]
• Samar Basu, Kristell Combe, Fabrice Kwiatkowski, Florence Caldefie-Chézet, Frédérique Penault-Llorca, Yves-Jean Bignon et al. Cellular Expression of Cyclooxygenase, Aromatase, Adipokines, Inflammation and Cell Proliferation Markers in Breast Cancer Specimen PLOS ONE 10/2/2015 [PMID: 26431176]
• Wu MH, Chuang PC, Chen HM Increased leptin expression in endometriosis cells is associated with endometrial stromal cell proliferation and leptin gene up-regulation. Mol. Hum. Reprod., 2002-05-01;8(5):456-64. 2002-05-01 [PMID: 11994543] (IHC-P, Western Blot, Human)

Bioinformatics

• Gene Symbol: LEP
• Uniprot:
  • Q6NT58()