Leptin/OB Antibody Pair [HRP]

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Sandwich ELISA: Leptin/OB Antibody Pair [HRP] [NBP2-79538] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, S-ELISA
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP
Standard Curve Range
7.81-500 pg/ml (example only; lot dependent)
Sensitivity
7.81 pg/ml (example only; lot dependent)

Order Details

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Catalog# & Conjugate Size Price

Leptin/OB Antibody Pair [HRP] Summary

Description
Solid Phase sandwich ELISA for the quantitative determination of Human Leptin/OB.
Specificity
This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Leptin/OB.
Standard Curve Range
7.81-500 pg/ml (example only; lot dependent)
Sensitivity
7.81 pg/ml (example only; lot dependent)
Assay Type
Sandwich ELISA
Sample Volume
100 ul
Clonality
Monoclonal
Host
Mouse
Gene
LEP

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
Application Notes
1. Capture Antibody: Dilute to a working concentration of 2 ug/ml in PBS before coating.
2. Detection Antibody: Dilute to working concentration of 1 ug/ml in detection antibody dilution buffer before use.
3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 500 pg/ml is recommended.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Capture Antibody: 0.5 mg/ml of mouse anti-Human Leptin/OB monoclonal antibody (in PBS, pH 7.4).
  2. Detection Antibody: 0.2 mg/ml of mouse anti-Human Leptin/OB monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  3. Standard: Each vial contains 172 ng of recombinant Human Leptin/OB.

Alternate Names for Leptin/OB Antibody Pair [HRP]

  • FLJ94114
  • LEP
  • leptin (murine obesity homolog)
  • leptin (obesity homolog, mouse)
  • Leptin
  • OB
  • Obese protein
  • obese, mouse, homolog of
  • Obesity factor
  • OBOBS

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol LEP