Leptin/OB Antibody (44804) [Biotin]

• Reactivity: Hu
• Applications: ELISA(Det)
• Clone: 44804
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: LYOPH

Leptin/OB Antibody (44804) [Biotin] Summary

• Immunogen: E. coli-derived recombinant human Leptin
  Val22-Cys167
  Accession # P41159
• Specificity: Detects human Leptin/OB in ELISAs and Western blots. In ELISAs, this antibody shows less than 0.1% cross-reactivity with recombinant mouse (rm) and rrLeptin. In Western blots, this antibody shows no cross-reactivity with rmLeptin.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LEP
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Human Leptin/OB Antibody (Catalog # MAB398)
  ELISA Detection: Human Leptin/OB Biotinylated Antibody (Catalog # BAM398)
  Standard: Recombinant Human Leptin/OB (Catalog # 398-LP)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Leptin/OB Antibody (44804) [Biotin]

• FLJ94114
• LEP
• leptin (murine obesity homolog)
• leptin (obesity homolog, mouse)
• Leptin
• OB
• Obese protein
• obese, mouse, homolog of
• Obesity factor
• OBOBS

Background

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the sythesis of Leptin have been found to be obese, diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Human Leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1 - 3). The roles of leptin in body weight regulation, hematopoiesis and reproduction continue to be investigated.

1. Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
2. Tartaglia, L.A. et al. (1995) Cell 83:1263.
3. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Leptin/OB Antibody (BAM398)(10)

Publications using BAM398

• Satish Patel, Anna Alvarez-Guaita, Audrey Melvin, Debra Rimmington, Alessia Dattilo, Emily L. Miedzybrodzka et al. GDF15 Provides an Endocrine Signal of Nutritional Stress in Mice and Humans Cell Metabolism 3/5/2019 [PMID: 30639358]
• Yuka Tomabechi, Takeshi Tsuruta, Shinichi Saito, Martin Wabitsch, Kei Sonoyama Extra-adrenal glucocorticoids contribute to the postprandial increase of circulating leptin in mice Journal of Cell Communication and Signaling 6/1/2018 [PMID: 28744834]
• Mok J, Mindrinos M, Davis R, Javanmard M Digital microfluidic assay for protein detection. Proc Natl Acad Sci U S A, 2014-01-21;111(6):2110-5. 2014-01-21 [PMID: 24449893] (Immunoassay Development, N/A)
• Dana C. Borcherding, Eric R. Hugo, Gila Idelman, Anuradha De Silva, Nathan W. Richtand, Jean Loftus et al. Dopamine Receptors in Human Adipocytes: Expression and Functions PLoS ONE 9/26/2011 [PMID: 21966540]
• Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E A multiplex immunoassay for human adipokine profiling. Clin. Chem., 2010-06-08;56(0):1320. 2010-06-08 [PMID: 20530731] (Luminex Development, Human)
• Hu H, Kwun J, Aizenstein BD, Knechtle SJ Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation, 2009-06-27;87(12):1814-20. 2009-06-27 [PMID: 19543058] (Antibody Array Development, Functional Assay, Human)
• Sabatier MJ, McCully KK, Marinik EL, Schwark EH, Haddow S, Cortez-Cooper M, Bergeron MF, Sloan GJ, Cannon JG Leptin, blood pressure, and aerobic capacity in women. Am. J. Hypertens., 2008-08-28;21(11):1245-50. 2008-08-28 [PMID: 18756258] (ELISA Development, Human)
• Emsley HC, Smith CJ, Gavin CM Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production. BMC Neurol, 2007-02-28;7(0):5. 2007-02-28 [PMID: 17328808] (ELISA Development, Human)
• Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA Steroids and protein markers in the follicular fluid as indicators of oocyte quality in patients with and without endometriosis. J. Assist. Reprod. Genet., 2005-06-01;22(6):257-64. 2005-06-01 [PMID: 16021855] (ELISA Development, Human)
• Kaser S, Kaser A, Vogel W, Patsch JR, Tilg H Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes. Eur. Cytokine Netw., 2002-04-01;13(2):225-9. 2002-04-01 [PMID: 12101078] (Human)

Bioinformatics

• Gene Symbol: LEP
• Uniprot:
  • P41159()