Leptin/OB Antibody (2299B) [Unconjugated]

Western blot shows lysates of mouse ovary tissue. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse Leptin/OB Monoclonal Antibody (Catalog # MAB498) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Leptin/OB at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Leptin/OB was detected in immersion fixed paraffin-embedded sections of mouse adipose tissue using Rabbit Anti-Mouse Leptin/OB Monoclonal Antibody (Catalog # MAB498) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in fat cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Recombinant Mouse Leptin/OB protein was serially diluted 2-fold and captured by Rabbit Anti-Mouse Leptin/OB Monoclonal Antibody (Catalog # MAB498) coated on a Clear Polystyrene Microplate (DY990). Rabbit Anti-Mouse Leptin/OB Monoclonal Antibody (MAB4981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

• Reactivity: Mu
• Applications: WB, ELISA, IHC
• Clone: 2299B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Leptin/OB Antibody (2299B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant mouse Leptin/OB
  Val22-Cys167
  Accession # Q544U0
• Specificity: Detects mouse Leptin/OB in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 3-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Leptin/OB Antibody (2299B) [Unconjugated]

• FLJ94114
• LEP
• leptin (murine obesity homolog)
• leptin (obesity homolog, mouse)
• Leptin
• OB
• Obese protein
• obese, mouse, homolog of
• Obesity factor
• OBOBS

Background

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. Mouse Leptin shares approximately 96% and 84% sequence identity with the rat and human protein, respectively. Mouse Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1-3). The possible roles of Leptin in body weight regulation, hematopoiesis and reproduction are being investigated.

1. Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
2. Tartaglia, L.A. et al. (1995) Cell 83:1263.
3. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Leptin/OB Antibody (MAB498)(1)

Publication using MAB498

• Q Cui, Y Zhang, N Tian, J Yang, D Ya, W Xiang, Z Zhou, Y Jiang, J Deng, B Yang, X Lin, Q Li, R Liao Leptin Promotes Angiogenesis via Pericyte STAT3 Pathway upon Intracerebral Hemorrhage Cells, 2022-09-03;11(17):. 2022-09-03 [PMID: 36078162] (In Vivo, Mouse)

Bioinformatics

• Uniprot:
  • Q544U0()