LC3 Antibody Pack

Images

 
Western Blot: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384.
Immunocytochemistry/ Immunofluorescence: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Immunofluorescent staining of treated U373-MG cells using NB600-1384. The nuclei were stained with DAPI.
Immunohistochemistry: LC3/MAP1LC3A Antibody Pack [NB910-40752] - LC3 staining in gliobastoma multiform tissue using NB600-1384.
Western Blot: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of LC3 in human brain lysate using NB 100-2220. Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo ...read more
Western Blot: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of autophagic LC3 in mouse ES cell lysate using NB 100-2331. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in ...read more
Immunohistochemistry: LC3/MAP1LC3A Antibody Pack [NB910-40752] - IHC staining of Brain, Cerebral Cortex, Neurons with Cell Processes 40x.
Immunohistochemistry: LC3/MAP1LC3A Antibody Pack [NB910-40752] - IHC staining of Brain, Cerebral Cortex, Cell Processes in Gray Matter 40x.
Immunohistochemistry: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Staining of treated U373-MG (human glioblastoma) cells using NB600-1384.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC

Order Details

LC3 Antibody Pack Summary

Gene
MAP1LC3B

Applications/Dilutions

Publications
Read Publications using
NB910-40752 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Kit Components

Components
  1. NB100-2220 (LC3B/MAP1LC3B antibody)
  2. NB100-2331 (LC3/MAP1LC3A Antibody)
  3. NB600-1384 (LC3B/MAP1LC3B Antibody)
  4. NB600-1384: LC3B Antibody
  5. NB100-2331: LC3A Antibody
  6. NB100-2220: LC3B Antibody

Notes

1 vial each of: NB100-2220 (0.1ml), NB100-2331 (0.1ml) and NB600-1384 (0.1ml).

Alternate Names for LC3 Antibody Pack

  • ATG8F
  • LC3B
  • MAP1A/1BLC3
  • map1lc3b
  • MAP1LC3B-a
  • microtubule associated protein 1 light chain 3 beta

Background

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associate with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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NB910-40752
Species: Hu, Mu
Applications: WB, ICC/IF, IHC

Publications for LC3 Antibody Pack (NB910-40752)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Blogs on LC3. Showing 1-10 of 35 blog posts - Show all blog posts.

Visualizing Autophagy by Microscopy
By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to vi...  Read full blog post.

Microglia: pruning shears for homeostatic maintenance in the brain
By Jennifer Sokolowski, MD, PhD.Microglia play a critical role in pruning neurons and synapses during homeostatic maintenance in the adult brain.1 A recent study by Ayata et al. (2018) identified regional differe...  Read full blog post.

The LC3 A, B, C’s and 1, 2, 3’s
By Christina Towers, PhD Autophagy is a catabolic process used to breakdown and recycle damaged proteins and organelles. It is a multistep process that, in its simplest form, consists of 4 steps: initiation, phagophor...  Read full blog post.

Best way to quantitatively measure Autophagic Flux
By Christina Towers, PhD Autophagy is a stress induced cellular recycling process that plays an important physiological role in many diseases. It is induced by a variety of stimuli both intracellular and extracellula...  Read full blog post.

Best Methods to Induce and Inhibit Autophagy Pharmacologically
By Christina Towers, PhD Autophagy facilitates the degradation and recycling of damaged cytoplasmic material. The multistep process consists of a double membrane structure called an autophagosome that engulfs proteins...  Read full blog post.

RNA-binding protein Staufen1 conspires with Atxn2 in stress granules to cause neurodegeneration by dysregulating RNA metabolism
By Jamshed Arslan Pharm.D. Spinocerebellar ataxia type 2 (SCA2) is a movement disorder characterized by neurodegeneration. The cause of this autosomal dominant disease is a mutation in the RNA processing gene Atxn2,...  Read full blog post.

Application Focus: I see an increase in LC3, now what?
By Christina Towers, PhD. Autophagy is highly conserved and tightly regulated process that all cell types use to recycle nutrients, particularly in the instance of stress1. As a result, even small changes in signali...  Read full blog post.

How a cell "reaches" out for help
By Christina Towers, PhD. Parkinson's disease is a debilitating neurodegenerative condition defined by the accumulation of alpha-synuclein-containing (alpha-SYN) intra-cytoplasmic inclusions, called Lewy bodies. The...  Read full blog post.

Measuring Autophagic Flux with LC3 protein levels: The do's and don'ts
By Christina Towers, PhD. Autophagy is a dynamic cellular recycling process that can be influenced by many different external and internal stimuli. The most commonly used assay to measure autophagy is a western blot f...  Read full blog post.

Monitoring Autophagy in Neurons
By Christina Towers, PhD. Autophagy is a critical cellular process used by most cells in the body to recycle nutrients and prevent harmful buildup of damaged proteins. It is particularly important in the brain, where...  Read full blog post.

Showing 1-10 of 35 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol MAP1LC3B