LAMP-2/CD107b Antibody (SA46-01)

Images

 
Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse placenta tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.
Western Blot: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of LAMP2a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The ...read more
Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-LAMP-2/CD107b antibody. The section was pre-treated using ...read more
Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human kidney tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human liver tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human pancreas tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse brain tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse kidney tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clone
SA46-01
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

LAMP-2/CD107b Antibody (SA46-01) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within C-terminal human LAMP-2/CD107b. (SwissProt: P13473 Human;SwissProt: P17047 Mouse;SwissProt: P17046 Rat)
Localization
Cell membrane, Endosome membrane, Lysosome membrane
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
LAMP2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:5000
Application Notes
IP-assay-dependent
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-67298 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for LAMP-2/CD107b Antibody (SA46-01)

  • CD107 antigen-like family member B
  • CD107b antigen
  • CD107b
  • LAMP2
  • LAMP-2
  • LAMP2A
  • LAMP-2A
  • LAMPB
  • LGP110
  • LGP-96
  • Lysosomal Associated Membrane Protein 2
  • lysosomal-associated membrane protein 2
  • lysosome-associated membrane glycoprotein 2
  • Lysosome-associated membrane protein 2

Background

LAMP-2 (Lysosome-associated membrane protein 2) is a single-pass type I membrane protein that belongs to a family of membrane glycoproteins (~40 KDa). LAMP-2 protein is encoded by nine exons, with the first 8 exons and a portion of exon 9 encoding the highly glycosylated protein domains within the lysosomal lumen. The transmembrane and cytosolic carboxy-terminal domains of LAMP-2 are encoded by the remaining sequence of exon 9 and conform the receptor for targeting proteins to the lysosome. Splicing of exon 9 in the LAMP-2 pre mRNA leads to various splice forms with distinct cytosolic domains. Three splice variants, LAMP-2A, -2B and -2C, have been identified which shuttle between the plasma membrane, endosomal compartment and lysosomes (1). Tissue specific expression has been described for each LAMP-2 splice variant, with LAMP-2A being more ubiquitously expressed (e.g., placenta, lung, liver, pancreas and prostate), LAMP-2B predominantly expressed in skeletal muscle and LAMP-2C in brain tissue (1). All LAMP-2 splice variants participate in lysosomal degradation processes. LAMP-2A is the only variant that serves as a receptor targeting proteins for lysosomal degradation in chaperone-mediated autophagy (2,3). LAMP-2B is essential for macroautophagy in cardiomyocytes, where it facilitates autophagosome-lysosome fusion. LAMP-2B mutations underscore the myopathy and severe hypertrophic cardiomyopathy in Danon disease which results from deficits in autophagy (1, 4). Vasculopathy of coronary and cerebral arteries is a rare phenotype in Danon patients that is also associated with deficient autophagy processing of proteins and cellular organelles (5). LAMP2C serves as a receptor for DNA and RNA, facilitating their lysosomal degradation through DNA-autophagy and RNA-autophagy, respectively (1).

References

1. Rowland, T. J., Sweet, M. E., Mestroni, L., & Taylor, M. R. G. (2016). Danon disease - dysregulation of autophagy in a multisystem disorder with cardiomyopathy. Journal of Cell Science. https://doi.org/10.1242/jcs.184770

2. Alfaro, I. E., Albornoz, A., Molina, A., Moreno, J., Cordero, K., Criollo, A., & Budini, M. (2019). Chaperone mediated autophagy in the crosstalk of neurodegenerative diseases and metabolic disorders. Frontiers in Endocrinology. https://doi.org/10.3389/fendo.2018.00778

3. Schneider, J. L., & Cuervo, A. M. (2014). Autophagy and human disease: Emerging themes. Current Opinion in Genetics and Development. https://doi.org/10.1016/j.gde.2014.04.003

4. Chi, C., Leonard, A., Knight, W. E., Beussman, K. M., Zhao, Y., Cao, Y., Song, K. (2019). LAMP-2B regulates human cardiomyocyte function by mediating autophagosome lysosome fusion. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.1808618116

5. Nguyen, H. T., Noguchi, S., Sugie, K., Matsuo, Y., Nguyen, C. T. H., Koito, H., Tsukaguchi, H. (2018). Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency. Scientific Reports. https://doi.org/10.1038/s41598-018-21602-8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-52721
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
7268-CT
Species: Hu
Applications: BA
DDX0191P-100
Species: Ca, Fe, Hu, Mu, Sh
Applications: ICC/IF, IHC, IHC-P
AF873
Species: Hu
Applications: WB
NBP2-42225
Species: Ca, Hu
Applications: B/N, DB, EM, ELISA, Flow-IC, Flow, Func, ICC/IF, IHC-WhMt, IP, In vitro, WB
AF1029
Species: Mu
Applications: ICC, IHC, IP, WB
NB100-2331
Species: Am, Ca, Fi, Hu, Mu, Pl, Rt, Ze
Applications: ChIP, ELISA, Flow, IB, ICC/IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, IP, Simple Western, SB, WB
NBP2-36568
Species: Hu, Mu
Applications: Flow-IC, ICC/IF, IHC, IHC-P, WB
H00338382-M01
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
NB400-129
Species: Ca, Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, IP, Simple Western, WB
AF953
Species: Hu
Applications: IHC, IP, KO, Simple Western, WB
AF3667
Species: Hu, Mu
Applications: ICC, IHC, Simple Western, WB
H00008878-M01
Species: Bv, Ha, Hu, Mu, Rb, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP1-87174
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
NB120-13253
Species: Bv, Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC-FrFl, IHC, IHC-P, IP, WB
137-PS
Species: Hu
Applications: BA
NBP1-25966
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP2-67298
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP

Publications for LAMP-2/CD107b Antibody (NBP2-67298)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for LAMP-2/CD107b Antibody (NBP2-67298) (0)

There are no reviews for LAMP-2/CD107b Antibody (NBP2-67298). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LAMP-2/CD107b Antibody (NBP2-67298) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional LAMP-2/CD107b Products

Bioinformatics Tool for LAMP-2/CD107b Antibody (NBP2-67298)

Discover related pathways, diseases and genes to LAMP-2/CD107b Antibody (NBP2-67298). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for LAMP-2/CD107b Antibody (NBP2-67298)

Discover more about diseases related to LAMP-2/CD107b Antibody (NBP2-67298).
 

Pathways for LAMP-2/CD107b Antibody (NBP2-67298)

View related products by pathway.

PTMs for LAMP-2/CD107b Antibody (NBP2-67298)

Learn more about PTMs related to LAMP-2/CD107b Antibody (NBP2-67298).
 

Research Areas for LAMP-2/CD107b Antibody (NBP2-67298)

Find related products by research area.

Blogs on LAMP-2/CD107b.

Chaperone Mediated Autophagy (CMA) does it all!
By Christina Towers, PhD. The degradation of cellular proteins is a critical step of both regulation and quality control and results in the turn over and recycling of critical amino acids. The two main mechanisms o...  Read full blog post.

LAMP2 - a marker of lysosomes and late endosomes
Lysosomes are membrane-bound organelles responsible for the degradation of various biological macromolecules. Vesicles containing hydrolytic enzymes bud from the Golgi and fuse with endosomes to form the mature lysosome capable of breaking down va...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our LAMP-2/CD107b Antibody (SA46-01) and receive a gift card or discount.

Bioinformatics

Gene Symbol LAMP2
Uniprot