NKG2D/CD314 Antibody (149810) [Unconjugated]

Images

 
Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2D/CD314 ...read more
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD8 APC-conjugated Monoclonal Antibody (FAB1509A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or ...read more
Multiple receptors and ligands are involved in NK cell-mediated lysis of activated CD4+ T cells.Role of (A) activating and (B) inhibitory NK receptors in NK cell degranulation. Left column: representative histograms (of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready, Func
Clone
149810
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

NKG2D/CD314 Antibody (149810) [Unconjugated] Summary

Additional Information
Clone 149810 was used by HLDA to establish CD designation
Immunogen
BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
Specificity
Detects human NKG2D/CD314 in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KLRK1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Costimulation of T Cells
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB139 in the following applications:

Publications
Read Publications using
MAB139 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKG2D/CD314 Antibody (149810) [Unconjugated]

  • CD314 antigen
  • CD314
  • D12S2489E
  • FLJ17759
  • FLJ75772
  • Killer cell lectin-like receptor subfamily K member 1
  • killer cell lectin-like receptor subfamily K, member 1
  • KLR
  • KLRK1
  • NK cell receptor D
  • NKG2-D type II integral membrane protein
  • NKG2D
  • NKG2-D
  • NKG2-D-activating NK receptor
  • NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

Background

NKG2D, also known as CD314, is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

  1. Li, P. et al. (2001) Nature Immunol. 2:443.
  2. Steinle, A. et al. (2001) Immunogenetics 53:279.
  3. Cosman, D. et al. (2001) Immunity 14:123.
  4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
  5. Wu, J. et al. (1999) Science 285:730.
  6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
  7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
  8. Groh, V. et al. (2001) Nature Immunol. 2:255.
  9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
  10. Diefenbach, A. et al. (2001) Nature 413:165.
  11. NKG2D and its Ligands (2002) www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKG2D/CD314 Antibody (MAB139)(85)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 12 applications: Bioassay, Blocking, Blocking/Neutralizing, Cytotoxicity Assay, Flow Cytometry, Functional Assay, ICC, IHC, IHC-Fr, Neutralization, Stimulation, Surface Plasmon Resonance.


Filter By Application
Bioassay
(5)
Blocking
(2)
Blocking/Neutralizing
(1)
Cytotoxicity Assay
(1)
Flow Cytometry
(12)
Functional Assay
(12)
ICC
(2)
IHC
(1)
IHC-Fr
(1)
Neutralization
(52)
Stimulation
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(85)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 85. Show All 85 Publications.
Publications using MAB139 Applications Species
King, LA;Toffoli, EC;Veth, M;Iglesias-Guimarais, V;Slot, MC;Amsen, D;van de Ven, R;Derks, S;Fransen, MF;Tuynman, JB;Riedl, T;Roovers, RC;Adang, AEP;Ruben, JM;Parren, PWHI;de Gruijl, TD;van der Vliet, HJ; A bispecific ?? T-cell engager targeting EGFR activates a potent V?9V?2 T cell-mediated immune response against EGFR-expressing tumors Cancer immunology research 2023-06-27 [PMID: 37368791] (Neutralization, Human) Neutralization Human
R Laufer Bri, A Keren, M Bertolini, Y Ullmann, R Paus, A Gilhar Involvement of ILC1-like innate lymphocytes in human autoimmunity, lessons from alopecia areata Elife, 2023-03-17;12(0):. 2023-03-17 [PMID: 36930216] (Neutralization, Human) Neutralization Human
A Carmena Mo, Y Carpentier, F Lemaître, N Farzam-Kia, S Da Cal, JV Guimond, E Haddad, P Duquette, JM Girard, A Prat, C Larochelle, N Arbour Specific alterations in NKG2D+ T lymphocytes in relapsing-remitting and progressive multiple sclerosis patients Multiple sclerosis and related disorders, 2023-01-26;71(0):104542. 2023-01-26 [PMID: 36716577] (Blocking/Neutralizing, Human) Blocking/Neutralizing Human
CK Yang, CH Huang, CH Hu, JH Fang, TC Chen, YC Lin, CY Lin Immunophenotype and antitumor activity of cytokine-induced killer cells from patients with hepatocellular carcinoma PLoS ONE, 2023-01-04;18(1):e0280023. 2023-01-04 [PMID: 36598909] (Neutralization, Human) Neutralization Human
MC Puzzolo, M Breccia, P Mariglia, G Colafigli, S Pepe, E Scalzulli, E Mariggiò, R Latagliata, A Guarini, R Foà Immunomodulatory Effects of IFNalpha on T and NK Cells in Chronic Myeloid Leukemia Patients in Deep Molecular Response Preparing for Treatment Discontinuation Journal of Clinical Medicine, 2022-09-23;11(19):. 2022-09-23 [PMID: 36233461] (Flow Cytometry, Human) Flow Cytometry Human
R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement Cell Reports, 2022-09-27;40(13):111423. 2022-09-27 [PMID: 36170817] (Neutralization, Human) Neutralization Human
S Belkahla, JM Brualla, A Fayd'herbe, P Falvo, N Allende-Ve, M Constantin, AUH Khan, L Coenon, C Alexia, G Mitola, P Massa, S Orecchioni, F Bertolini, W Mnif, J Hernandez, A Anel, M Villalba The metabolism of cells regulates their sensitivity to NK cells depending on p53 status Scientific Reports, 2022-02-25;12(1):3234. 2022-02-25 [PMID: 35217717] (Neutralization, Human) Neutralization Human
N Allende-Ve, J Marco Brua, P Falvo, C Alexia, M Constantin, AF de Maudave, L Coenon, D Gitenay, G Mitola, P Massa, S Orecchioni, F Bertolini, I Marzo, A Anel, M Villalba Metformin sensitizes leukemic cells to cytotoxic lymphocytes by increasing expression of intercellular adhesion molecule-1 (ICAM-1) Scientific Reports, 2022-01-25;12(1):1341. 2022-01-25 [PMID: 35079096] (Neutralization, Human) Neutralization Human
HY Wu, KX Li, WY Pan, MQ Guo, DZ Qiu, YJ He, YH Li, YX Huang Venetoclax enhances NK cell killing sensitivity of AML cells through the NKG2D/NKG2DL activation pathway International immunopharmacology, 2022-01-06;104(0):108497. 2022-01-06 [PMID: 34999394] (Neutralization, Human) Neutralization Human
D Westhoff S, A Chakravort, M Hayes, W Hammerschm, B Sugden The Epstein-Barr Virus Oncogene EBNA1 Suppresses Natural Killer Cell Responses and Apoptosis Early after Infection of Peripheral B Cells MBio, 2021-11-16;0(0):e0224321. 2021-11-16 [PMID: 34781735] (Neutralization, Human) Neutralization Human
Show All 85 Publications.

Review for NKG2D/CD314 Antibody (MAB139) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB139:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot NKG2D/CD314 MAB139
Enlarge
5
reviewed by:
Verified Customer
WB Human 01/28/2022
View

Summary

ApplicationWestern Blot
Sample TestedPeripheral blood mononuclear cells (PBMCs)
SpeciesHuman
Lot149810

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NKG2D/CD314 Antibody (MAB139) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional NKG2D/CD314 Products

Blogs on NKG2D/CD314.

Harnessing Natural Killer Cell Activity for Anti-Tumor Immunotherapy
By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...  Read full blog post.

mFluor Violet Conjugated Antibodies

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
01/28/2022
Application: WB
Species: Human

Bioinformatics

Gene Symbol KLRK1