NKG2D/CD314 Antibody (149810) [Unconjugated]


Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2D/CD314 ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready, Func

Order Details

NKG2D/CD314 Antibody (149810) [Unconjugated] Summary

Additional Information
Clone 149810 was used by HLDA to establish CD designation
BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
Detects human NKG2D/CD314 in Western blots.
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Costimulation of T Cells
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization
  • Western Blot 1 ug/mL
Read Publications using
MAB139 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKG2D/CD314 Antibody (149810) [Unconjugated]

  • CD314 antigen
  • CD314
  • D12S2489E
  • FLJ17759
  • FLJ75772
  • Killer cell lectin-like receptor subfamily K member 1
  • killer cell lectin-like receptor subfamily K, member 1
  • KLR
  • KLRK1
  • NK cell receptor D
  • NKG2-D type II integral membrane protein
  • NKG2D
  • NKG2-D
  • NKG2-D-activating NK receptor
  • NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence


NKG2D, also known as CD314, is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

  1. Li, P. et al. (2001) Nature Immunol. 2:443.
  2. Steinle, A. et al. (2001) Immunogenetics 53:279.
  3. Cosman, D. et al. (2001) Immunity 14:123.
  4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
  5. Wu, J. et al. (1999) Science 285:730.
  6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
  7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
  8. Groh, V. et al. (2001) Nature Immunol. 2:255.
  9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
  10. Diefenbach, A. et al. (2001) Nature 413:165.
  11. NKG2D and its Ligands (2002) www.RnDSystems.com.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKG2D/CD314 Antibody (MAB139)(73)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: Bioassay, Blocking, Flow Cytometry, Functional Assay, ICC, Neutralization, Surface Plasmon Resonance.

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Showing Publications 1 - 10 of 73. Show All 73 Publications.
Publications using MAB139 Applications Species
X Zhong, H Liao, S Hu, K Luo, H Zhu The diterpenoid adenanthin upregulates the expression of natural killer group 2D receptor ligands in hepatocellular carcinoma cells Molecular and cellular probes, 2021;59(0):101759. 2021 [PMID: 34265372] (Flow Cytometry, Human) Flow Cytometry Human
E Seidel, L Dassa, C Schuler, E Oiknine-Dj, DG Wolf, VTK Le-Trillin, O Mandelboim The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing PloS Pathogens, 2021;17(5):e1008807. 2021 [PMID: 33939764] (Neutralization, Human) Neutralization Human
M Diab, D Schmiedel, E Seidel, E Bacharach, O Mandelboim Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D Viruses, 2020;12(7):. 2020 [PMID: 32698530] (Neutralization, Human) Neutralization Human
S Capellero, J Erriquez, C Melano, G Mesiano, S Genta, A Pisacane, G Mittica, E Ghisoni, M Olivero, MF Di Renzo, M Aglietta, D Sangiolo, G Valabrega Preclinical immunotherapy with Cytokine-Induced Killer lymphocytes against epithelial ovarian cancer Sci Rep, 2020;10(1):6478. 2020 [PMID: 32296104] (Neutralization, Human) Neutralization Human
E Oh, B Min, Y Li, C Lian, J Hong, GM Park, B Yang, SY Cho, YK Hwang, CO Yun Cryopreserved Human Natural Killer Cells Exhibit Potent Antitumor Efficacy against Orthotopic Pancreatic Cancer through Efficient Tumor-Homing and Cytolytic Ability (Running Title: Cryopreserved NK Cells Exhibit Antitumor Effect) Cancers (Basel), 2019;11(7):. 2019 [PMID: 31324057] (Neutralization, Human) Neutralization Human
A Gras Navar, H Espedal, JV Joseph, L Trachsel-M, M Bahador, B Tore Gjert, E Klæboe Kri, A Simonsen, H Miletic, P Øyvind Eng, MA Rahman, M Chekenya Pretreatment of Glioblastoma with Bortezomib Potentiates Natural Killer Cell Cytotoxicity through TRAIL/DR5 Mediated Apoptosis and Prolongs Animal Survival Cancers (Basel), 2019;11(7):. 2019 [PMID: 31319548] (Neutralization, Human) Neutralization Human
HG Wirsching, H Zhang, F Szulzewsky, S Arora, P Grandi, PJ Cimino, N Amankulor, JS Campbell, L McFerrin, SS Pattwell, C Ene, A Hicks, M Ball, J Yan, J Zhang, D Kumasaka, RH Pierce, M Weller, M Finer, C Quéva, JC Glorioso, AM Houghton, EC Holland Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma JCI Insight, 2019;4(13):. 2019 [PMID: 31292299] (Flow Cytometry, Human) Flow Cytometry Human
CF Amorim, NB Carvalho, JA Neto, SB Santos, MFR Grassi, LP Carvalho, EM Carvalho The Role of NK Cells in the Control of Viral Infection in HTLV-1 Carriers J Immunol Res, 2019;2019(0):6574828. 2019 [PMID: 30944834] (Neutralization, Human) Neutralization Human
B Müller-Dur, J Grählert, OP Devine, AN Akbar, C Hess CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age Aging (Albany NY), 2019;11(2):724-740. 2019 [PMID: 30686790] (Flow Cytometry, Human) Flow Cytometry Human
J Prévost, J Richard, H Medjahed, A Alexander, J Jones, JC Kappes, C Ochsenbaue, A Finzi Incomplete downregulation of CD4 expression affects HIV-1 Env conformation and ADCC responses J. Virol., 2018;0(0):. 2018 [PMID: 29669829] (Neutralization, Human) Neutralization Human
Show All 73 Publications.

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB139U
Alexa Fluor 405 FAB139V
Alexa Fluor 488 FAB139G
Alexa Fluor 488 FAB139G
Alexa Fluor 532 FAB139X
Alexa Fluor 594 FAB139T
Alexa Fluor 647 FAB139R
Alexa Fluor 700 FAB139N
Alexa Fluor 700 FAB139N
Alexa Fluor 750 FAB139S
Allophycocyanin FAB139A
Allophycocyanin FAB139A
Biotin FAB139B
DyLight 350 FAB139D
DyLight 405 FAB139E
DyLight 488 FAB139K
DyLight 550 FAB139L
DyLight 594 FAB139M
DyLight 650 FAB139W
DyLight 680 FAB139Y
DyLight 755 FAB139Z
Janelia Fluor 549 FAB139I
Janelia Fluor 646 FAB139J
Phycoerythrin FAB139P
Phycoerythrin FAB139P

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Blogs on NKG2D/CD314.

Harnessing Natural Killer Cell Activity for Anti-Tumor Immunotherapy
By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...  Read full blog post.

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Gene Symbol KLRK1