NKG2D/CD314 Antibody (149810) [Unconjugated]


Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2D/CD314 ...read more
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD8 APC-conjugated Monoclonal Antibody (FAB1509A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or ...read more
Multiple receptors and ligands are involved in NK cell-mediated lysis of activated CD4+ T cells.Role of (A) activating and (B) inhibitory NK receptors in NK cell degranulation. Left column: representative histograms (of ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready, Func

Order Details

NKG2D/CD314 Antibody (149810) [Unconjugated] Summary

Additional Information
Clone 149810 was used by HLDA to establish CD designation
BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
Detects human NKG2D/CD314 in Western blots.
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Costimulation of T Cells
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
MAB139 in the following applications:

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MAB139 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKG2D/CD314 Antibody (149810) [Unconjugated]

  • CD314 antigen
  • CD314
  • D12S2489E
  • FLJ17759
  • FLJ75772
  • Killer cell lectin-like receptor subfamily K member 1
  • killer cell lectin-like receptor subfamily K, member 1
  • KLR
  • KLRK1
  • NK cell receptor D
  • NKG2-D type II integral membrane protein
  • NKG2D
  • NKG2-D
  • NKG2-D-activating NK receptor
  • NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence


NKG2D, also known as CD314, is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

  1. Li, P. et al. (2001) Nature Immunol. 2:443.
  2. Steinle, A. et al. (2001) Immunogenetics 53:279.
  3. Cosman, D. et al. (2001) Immunity 14:123.
  4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
  5. Wu, J. et al. (1999) Science 285:730.
  6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
  7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
  8. Groh, V. et al. (2001) Nature Immunol. 2:255.
  9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
  10. Diefenbach, A. et al. (2001) Nature 413:165.
  11. NKG2D and its Ligands (2002) www.RnDSystems.com.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKG2D/CD314 Antibody (MAB139)(85)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 12 applications: Bioassay, Blocking, Blocking/Neutralizing, Cytotoxicity Assay, Flow Cytometry, Functional Assay, ICC, IHC, IHC-Fr, Neutralization, Stimulation, Surface Plasmon Resonance.

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Showing Publications 1 - 10 of 85. Show All 85 Publications.
Publications using MAB139 Applications Species
King, LA;Toffoli, EC;Veth, M;Iglesias-Guimarais, V;Slot, MC;Amsen, D;van de Ven, R;Derks, S;Fransen, MF;Tuynman, JB;Riedl, T;Roovers, RC;Adang, AEP;Ruben, JM;Parren, PWHI;de Gruijl, TD;van der Vliet, HJ; A bispecific ?? T-cell engager targeting EGFR activates a potent V?9V?2 T cell-mediated immune response against EGFR-expressing tumors Cancer immunology research 2023-06-27 [PMID: 37368791] (Neutralization, Human) Neutralization Human
R Laufer Bri, A Keren, M Bertolini, Y Ullmann, R Paus, A Gilhar Involvement of ILC1-like innate lymphocytes in human autoimmunity, lessons from alopecia areata Elife, 2023-03-17;12(0):. 2023-03-17 [PMID: 36930216] (Neutralization, Human) Neutralization Human
A Carmena Mo, Y Carpentier, F Lemaître, N Farzam-Kia, S Da Cal, JV Guimond, E Haddad, P Duquette, JM Girard, A Prat, C Larochelle, N Arbour Specific alterations in NKG2D+ T lymphocytes in relapsing-remitting and progressive multiple sclerosis patients Multiple sclerosis and related disorders, 2023-01-26;71(0):104542. 2023-01-26 [PMID: 36716577] (Blocking/Neutralizing, Human) Blocking/Neutralizing Human
CK Yang, CH Huang, CH Hu, JH Fang, TC Chen, YC Lin, CY Lin Immunophenotype and antitumor activity of cytokine-induced killer cells from patients with hepatocellular carcinoma PLoS ONE, 2023-01-04;18(1):e0280023. 2023-01-04 [PMID: 36598909] (Neutralization, Human) Neutralization Human
MC Puzzolo, M Breccia, P Mariglia, G Colafigli, S Pepe, E Scalzulli, E Mariggiò, R Latagliata, A Guarini, R Foà Immunomodulatory Effects of IFNalpha on T and NK Cells in Chronic Myeloid Leukemia Patients in Deep Molecular Response Preparing for Treatment Discontinuation Journal of Clinical Medicine, 2022-09-23;11(19):. 2022-09-23 [PMID: 36233461] (Flow Cytometry, Human) Flow Cytometry Human
R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement Cell Reports, 2022-09-27;40(13):111423. 2022-09-27 [PMID: 36170817] (Neutralization, Human) Neutralization Human
S Belkahla, JM Brualla, A Fayd'herbe, P Falvo, N Allende-Ve, M Constantin, AUH Khan, L Coenon, C Alexia, G Mitola, P Massa, S Orecchioni, F Bertolini, W Mnif, J Hernandez, A Anel, M Villalba The metabolism of cells regulates their sensitivity to NK cells depending on p53 status Scientific Reports, 2022-02-25;12(1):3234. 2022-02-25 [PMID: 35217717] (Neutralization, Human) Neutralization Human
N Allende-Ve, J Marco Brua, P Falvo, C Alexia, M Constantin, AF de Maudave, L Coenon, D Gitenay, G Mitola, P Massa, S Orecchioni, F Bertolini, I Marzo, A Anel, M Villalba Metformin sensitizes leukemic cells to cytotoxic lymphocytes by increasing expression of intercellular adhesion molecule-1 (ICAM-1) Scientific Reports, 2022-01-25;12(1):1341. 2022-01-25 [PMID: 35079096] (Neutralization, Human) Neutralization Human
HY Wu, KX Li, WY Pan, MQ Guo, DZ Qiu, YJ He, YH Li, YX Huang Venetoclax enhances NK cell killing sensitivity of AML cells through the NKG2D/NKG2DL activation pathway International immunopharmacology, 2022-01-06;104(0):108497. 2022-01-06 [PMID: 34999394] (Neutralization, Human) Neutralization Human
D Westhoff S, A Chakravort, M Hayes, W Hammerschm, B Sugden The Epstein-Barr Virus Oncogene EBNA1 Suppresses Natural Killer Cell Responses and Apoptosis Early after Infection of Peripheral B Cells MBio, 2021-11-16;0(0):e0224321. 2021-11-16 [PMID: 34781735] (Neutralization, Human) Neutralization Human
Show All 85 Publications.

Review for NKG2D/CD314 Antibody (MAB139) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB139:
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Images Ratings Applications Species Date Details
Western Blot NKG2D/CD314 MAB139
reviewed by:
Verified Customer
WB Human 01/28/2022


ApplicationWestern Blot
Sample TestedPeripheral blood mononuclear cells (PBMCs)

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Verified Customer
Application: WB
Species: Human


Gene Symbol KLRK1