NKG2D/CD314 Antibody (149810)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready, Func
Clone
149810
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

NKG2D/CD314 Antibody (149810) Summary

Additional Information
Clone 149810 was used by HLDA to establish CD designation
Immunogen
BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
Specificity
Detects human NKG2D/CD314 in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KLRK1
Purity
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Costimulation of T Cells
  • CyTOF-ready
  • Neutralization
Publications
Read Publications using
MAB139 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKG2D/CD314 Antibody (149810)

  • CD314 antigen
  • CD314
  • D12S2489E
  • FLJ17759
  • FLJ75772
  • Killer cell lectin-like receptor subfamily K member 1
  • killer cell lectin-like receptor subfamily K, member 1
  • KLR
  • KLRK1
  • NK cell receptor D
  • NKG2-D type II integral membrane protein
  • NKG2D
  • NKG2-D
  • NKG2-D-activating NK receptor
  • NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

Background

NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

  1. Li, P. et al. (2001) Nature Immunol. 2:443.
  2. Steinle, A. et al. (2001) Immunogenetics 53:279.
  3. Cosman, D. et al. (2001) Immunity 14:123.
  4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
  5. Wu, J. et al. (1999) Science 285:730.
  6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
  7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
  8. Groh, V. et al. (2001) Nature Immunol. 2:255.
  9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
  10. Diefenbach, A. et al. (2001) Nature 413:165.
  11. NKG2D and its Ligands (2002) www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKG2D/CD314 Antibody (MAB139)(68)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: Bioassay, Blocking, Flow Cytometry, Functional Assay, ICC, Neutralization, Surface Plasmon Resonance.


Filter By Application
Bioassay
(5)
Blocking
(2)
Flow Cytometry
(10)
Functional Assay
(12)
ICC
(1)
Neutralization
(37)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(68)
All Species
Showing Publications 1 - 10 of 68. Show All 68 Publications.
Publications using MAB139 Applications Species
E Oh, B Min, Y Li, C Lian, J Hong, GM Park, B Yang, SY Cho, YK Hwang, CO Yun Cryopreserved Human Natural Killer Cells Exhibit Potent Antitumor Efficacy against Orthotopic Pancreatic Cancer through Efficient Tumor-Homing and Cytolytic Ability (Running Title: Cryopreserved NK Cells Exhibit Antitumor Effect) Cancers (Basel), 2019;11(7):. 2019 [PMID: 31324057] (Neutralization, Human) Neutralization Human
A Gras Navar, H Espedal, JV Joseph, L Trachsel-M, M Bahador, B Tore Gjert, E Klæboe Kri, A Simonsen, H Miletic, P Øyvind Eng, MA Rahman, M Chekenya Pretreatment of Glioblastoma with Bortezomib Potentiates Natural Killer Cell Cytotoxicity through TRAIL/DR5 Mediated Apoptosis and Prolongs Animal Survival Cancers (Basel), 2019;11(7):. 2019 [PMID: 31319548] (Neutralization, Human) Neutralization Human
HG Wirsching, H Zhang, F Szulzewsky, S Arora, P Grandi, PJ Cimino, N Amankulor, JS Campbell, L McFerrin, SS Pattwell, C Ene, A Hicks, M Ball, J Yan, J Zhang, D Kumasaka, RH Pierce, M Weller, M Finer, C Quéva, JC Glorioso, AM Houghton, EC Holland Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma JCI Insight, 2019;4(13):. 2019 [PMID: 31292299] (Flow Cytometry, Human) Flow Cytometry Human
CF Amorim, NB Carvalho, JA Neto, SB Santos, MFR Grassi, LP Carvalho, EM Carvalho The Role of NK Cells in the Control of Viral Infection in HTLV-1 Carriers J Immunol Res, 2019;2019(0):6574828. 2019 [PMID: 30944834] (Neutralization, Human) Neutralization Human
B Müller-Dur, J Grählert, OP Devine, AN Akbar, C Hess CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age Aging (Albany NY), 2019;11(2):724-740. 2019 [PMID: 30686790] (Flow Cytometry, Human) Flow Cytometry Human
YS Lee, W Heo, J Nam, YH Jeung, J Bae The combination of ionizing radiation and proteasomal inhibition by bortezomib enhances the expression of NKG2D ligands in multiple myeloma cells J. Radiat. Res., 2018;0(0):. 2018 [PMID: 29518205] (Neutralization, Human) Neutralization Human
YM Dai, HY Liu, YF Liu, Y Zhang, W He EBV transformation induces overexpression of hMSH2/3/6 on B lymphocytes and enhances ??T cell-mediated cytotoxicity via TCR and NKG2D Immunology, 2018;0(0):. 2018 [PMID: 29512904] (Neutralization, Human) Neutralization Human
O Matalon, A Ben-Shmuel, J Kivelevitz, B Sabag, S Fried, N Joseph, E Noy, G Biber, M Barda-Saad Actin retrograde flow controls natural killer cell response by regulating the conformation state of SHP-1 EMBO J., 2018;0(0):. 2018 [PMID: 29449322] (Functional Assay, Human) Functional Assay Human
S López-Cobo, N Pieper, C Campos-Sil, EM García-Cue, HT Reyburn, A Paschen, M Valés-Góme Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors Oncoimmunology, 2018;7(2):e1392426. 2018 [PMID: 29308322] (Bioassay, Human) Bioassay Human
Y Charpak-Am, T Kubsch, E Seidel, E Oiknine-Dj, N Cavaletto, R Yamin, D Schmiedel, D Wolf, G Gribaudo, M Messerle, L Cicin-Sain, O Mandelboim Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20 Sci Rep, 2017;7(1):8661. 2017 [PMID: 28819195] (Neutralization, Human) Neutralization Human
Show All 68 Publications.

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Bioinformatics

Gene Symbol KLRK1