NKG2D/CD314 Antibody (149810) [Unconjugated]

Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by anti-Mouse IgG PE-conjugated Monoclonal Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD8 APC-conjugated Monoclonal Antibody (FAB1509A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or (B) Mouse IgG1 Isotype Control (MAB002) followed by anti-Mouse IgG PE-conjugated Monoclonal Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Multiple receptors and ligands are involved in NK cell-mediated lysis of activated CD4+ T cells.Role of (A) activating and (B) inhibitory NK receptors in NK cell degranulation. Left column: representative histograms (of n≥3) for surface expression of ligands on activated (thick black line) and resting CD4+ T cells (thin black line). Isotype-matched control Ig are represented by dashed line (activated CD4+ T) and filled histogram (resting CD4+ T). Middle- and right column: NK and CD4+ T cells were activated for 4 days in vitro as described, and co-cultured for 4 hours with 10 ug/mL mAb (or relevant isotype-matched control Ig). Degranulation is shown for CD56dim (middle column) and CD56bright (right column) NK cells. Representative histograms of surface expression of receptors on activated (thick black line) and resting NK cells (thin black line). Isotype-matched control Ig are represented by dashed line (activated NK) and filled histogram (resting NK). * P<0.05, ** P<0.005, *** P<0.001. (C) Sorted IL-2-activated CD56dim and CD56bright NK cells were co-cultured with 51Cr-labeled activated CD4+ T cells in a 51Cr-release assay with human IgG4 isotype control (•) or anti-NKG2A mAb (○). Data represents n = 3 experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22384114), licensed under a CC-BY license. Not internally tested by R&D Systems.

CD4+NKG2D+ T cells exert pro-migratory, cytolytic and pro-inflammatory properties.(A) Flow cytometry: The dot plot shows a representative example of NKG2D expression on CD3+CD4+CD8-CD56− T cells derived from the peripheral blood of a healthy donor (HD). The bar graph represents the mean frequency of CD4+NKG2D+ T cells in the peripheral blood of HDs (n = 20), stable RRMS (n = 15) and active RRMS patients (n = 14). (B–E) Mean fluorescence intensity (MFI) of different markers indicative for migratory capacity (B), activation (C), or cytolytic capacity (D, E) of CD4+NKG2D+ and CD4+NKG2D− T cells from the peripheral blood of HDs (n = 6) or active RRMS patients (n = 6). (F) Percentages of naive (CD45RA+CD62L+), T central memory (Tcm, CD45RA-CD62L+), T effector memory (Tem, CD45RA-CD62L−) and T effector memory RA (Tem-RA, CD45RA+CD62L-) cells in the CD4+NKG2D+ and CD4+NKG2D− T cell compartment assessed by flow cytometry (n = 6 HDs). (G) Carboxyfluorescein succinimidyl ester (CFSE) proliferation assays of CD4+NKG2D+ T cells and CD4+NKG2D− T cells under CD3/CD28, CD3/NKG2D, MOG35-55 (10 µg/ml or 100 µg/ml), MBP1-11 or PLP190-209 stimulation (n = 8 HDs). (H) Intracellular cytokine staining for IFN-gamma and IL-17 of CD4+NKG2D+ and CD4+NKG2D− T cells derived from the peripheral blood of HDs (n = 7). The dot plots depict a representative example of IFN-gamma - and IL-17-positive cells upon CD3/CD28 stimulation. The bar graphs show the frequencies of IFN-gamma or IL-17 positive cells of unstimulated, CD3/CD28- or CD3/NKG2D-stimulated cells. (I) Comparison of the proportions of IFN-gamma or IL-17 positive CD3/CD28-stimulated CD4NKG2D+ T cells derived from frozen PBMCs of HDs (n = 6) or active RRMS patients (n = 6). *P < 0.05. ns, not significant; unstim., unstimulated. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24282598), licensed under a CC-BY license. Not internally tested by R&D Systems.

NKG2D and LFA-1 ligation trigger morphologically different responses in NK cells. (H) Quantification of f-actin (phalloidin-AlexaFluor633) across the diameter of cells stimulated on anti-NKG2D (solid line) or anti-LFA-1 (dotted line) (horizontal bars indicate p<0.05 by ANOVA, n = 20). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/19636352), licensed under a CC-BY license. Not internally tested by R&D Systems.

Consequences of MICA-mediated and antibody-mediated NKG2D engagement on cell activation, NKG2D and TIGIT expression. Primary cultured NK cells were incubated with plate-bound recombinant MICA Fc-chimera or anti-NKG2D mAb and goat anti-mouse antibody (GAM). GAM-stimulated NK cells were used as control of NKG2D stimulation. (A) Cells were harvested after 4 hours of stimulation, and degranulation was measured via flow cytometry by assessing CD107a endosomal marker expression. A representative experiment is shown in left panel while means ± SD of three independent experiments are shown in right panel. Paired t test was used for comparisons. *P < 0.05. (B, C) Surface expression of NKG2D and TIGIT receptors was evaluated by FACS analysis upon 18 hours of stimulation. One representative experiment is shown in left panels. Right panels: data pooled from six independent experiments (mean ± SD) are shown (the mean fluorescence intensity, MFI, value of controls NK cells in each experiment is set to 1). Paired t test was used for comparisons. *ρ < 0.05, **ρ < 0.01, ***ρ < 0.001. (D) Relative TIGIT mRNA amount, normalized with GAPDH, was expressed as arbitrary units and referred to the relative controls, considered as calibrator (the value of the calibrator in each run is set to 1). Means ± SD of five independent experiments are shown. Paired t test was used for comparisons. *ρ < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40421025), licensed under a CC-BY license. Not internally tested by R&D Systems.

NKG2D stimulation impairs DNAM-1-triggered ERK1/2 phosphorylation. (A, B) goat anti-mouse (GAM) and anti-NKG2D-stimulated NK cells were incubated with anti-DNAM-1 mAb or isotype control Ab followed by goat anti-mouse Ab for different lengths of times. Then, cells were fixed and permeabilized, stained with anti-pAKT (panel A) and anti-pERK1/2 (panel B) and analyzed by FACS. Results from 2 minutes of stimulation with anti-DNAM-1 mAb (filled histograms) or isotype control Ab (empty histograms) from one representative of three independent experiments is shown in the left panels. Quantitative changes in protein phosphorylation at the indicated lengths of time of three independent experiments (mean ± SD) are shown in the right panels. The ratio between the mean fluorescence intensity (MFI) of anti-DNAM-1-stimulated and Ctrl-Ig samples for each time point was used to calculate fold changes. Multiple t test was used for comparisons. *ρ < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40421025), licensed under a CC-BY license. Not internally tested by R&D Systems.

NKG2D internalization is required for impairment in DNAM-1-mediated cytotoxic granule polarization. (A) Surface expression of NKG2D (upper panels) and TIGIT (lower panels) receptors was evaluated on goat anti-mouse (GAM) or anti-NKG2D-stimulated DAP10 WT and DAP10K84R NKL. One representative of three independent experiments is shown in left panels. Data pooled from three independent experiments (mean ± SD) are shown in right panels (the mean fluorescence intensity, MFI value of GAM-stimulated cells in each experiment is set to 1). Paired t test was used for comparisons. *ρ < 0.05. (B) GAM and anti-NKG2D-stimulated DAP10 WT and DAP10K84R NKL (effector cells, E) were incubated with Ba/F3-PVR (target cells, T) for 30 minutes, fixed, permeabilized and stained with the anti-Perforin mAb followed by AlexaFluor594-conjugated GAM-IgG2b and AlexaFluor488-conjugated Phalloidin to visualize polarized actin. Cells were then counterstained with DAPI to visualize nuclei and acquired with LSM980 confocal microscope with a 63x/1.4 NA UPlanSAPO oil immersion objective (all from Zeiss). Right panel: Percentages of conjugates with polarized granules are shown as mean ± SD calculated on ≥30 conjugates for each NK cell type of three independent experiments. Paired t test was used for comparisons. *ρ < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40421025), licensed under a CC-BY license. Not internally tested by R&D Systems.

NKG2D stimulation impairs DNAM-1-triggered ERK1/2 phosphorylation. (A, B) goat anti-mouse (GAM) and anti-NKG2D-stimulated NK cells were incubated with anti-DNAM-1 mAb or isotype control Ab followed by goat anti-mouse Ab for different lengths of times. Then, cells were fixed and permeabilized, stained with anti-pAKT (panel A) and anti-pERK1/2 (panel B) and analyzed by FACS. Results from 2 minutes of stimulation with anti-DNAM-1 mAb (filled histograms) or isotype control Ab (empty histograms) from one representative of three independent experiments is shown in the left panels. Quantitative changes in protein phosphorylation at the indicated lengths of time of three independent experiments (mean ± SD) are shown in the right panels. The ratio between the mean fluorescence intensity (MFI) of anti-DNAM-1-stimulated and Ctrl-Ig samples for each time point was used to calculate fold changes. Multiple t test was used for comparisons. *ρ < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40421025), licensed under a CC-BY license. Not internally tested by R&D Systems.

NKG2D internalization is required for impairment in DNAM-1-mediated cytotoxic granule polarization. (A) Surface expression of NKG2D (upper panels) and TIGIT (lower panels) receptors was evaluated on goat anti-mouse (GAM) or anti-NKG2D-stimulated DAP10 WT and DAP10K84R NKL. One representative of three independent experiments is shown in left panels. Data pooled from three independent experiments (mean ± SD) are shown in right panels (the mean fluorescence intensity, MFI value of GAM-stimulated cells in each experiment is set to 1). Paired t test was used for comparisons. *ρ < 0.05. (B) GAM and anti-NKG2D-stimulated DAP10 WT and DAP10K84R NKL (effector cells, E) were incubated with Ba/F3-PVR (target cells, T) for 30 minutes, fixed, permeabilized and stained with the anti-Perforin mAb followed by AlexaFluor594-conjugated GAM-IgG2b and AlexaFluor488-conjugated Phalloidin to visualize polarized actin. Cells were then counterstained with DAPI to visualize nuclei and acquired with LSM980 confocal microscope with a 63x/1.4 NA UPlanSAPO oil immersion objective (all from Zeiss). Right panel: Percentages of conjugates with polarized granules are shown as mean ± SD calculated on ≥30 conjugates for each NK cell type of three independent experiments. Paired t test was used for comparisons. *ρ < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40421025), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Flow, B/N, CyTOF-ready, Func
• Clone: 149810
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NKG2D/CD314 Antibody (149810) [Unconjugated] Summary

• Additional Information: Clone 149810 was used by HLDA to establish CD designation
• Immunogen: BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
• Specificity: Detects human NKG2D/CD314 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLRK1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Costimulation of T Cells
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKG2D/CD314 Antibody (149810) [Unconjugated]

• CD314 antigen
• CD314
• D12S2489E
• FLJ17759
• FLJ75772
• Killer cell lectin-like receptor subfamily K member 1
• killer cell lectin-like receptor subfamily K, member 1
• KLR
• KLRK1
• NK cell receptor D
• NKG2-D type II integral membrane protein
• NKG2D
• NKG2-D
• NKG2-D-activating NK receptor
• NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

Background

NKG2D, also known as CD314, is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8⁺ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

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2. Steinle, A. et al. (2001) Immunogenetics 53:279.
3. Cosman, D. et al. (2001) Immunity 14:123.
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5. Wu, J. et al. (1999) Science 285:730.
6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
8. Groh, V. et al. (2001) Nature Immunol. 2:255.
9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
10. Diefenbach, A. et al. (2001) Nature 413:165.
11. NKG2D and its Ligands (2002) www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKG2D/CD314 Antibody (MAB139)(104)

Publications using MAB139

• Sproviero, E;Gnocchini, E;Cipollone, T;Petillo, S;Cassone, C;Molfetta, R;Zingoni, A;Soriani, A;Cerboni, C;Petrucci, MT;Fazio, F;Paolini, R;Palmieri, G;Cippitelli, M; Targeting RNA polymerase I to boost natural killer cell anticancer activity in multiple myeloma Cell death & disease 2025-11-28 [PMID: 41315184] (Flow Cytometry, Human)
• Jürgens, M;Claus, M;Wingert, S;Niemann, JA;Picard, LK;Hennes, E;Haasler, I;Hellwig, B;Overbeck, N;Reinders, J;Rahnenführer, J;Schedel, M;Capellino, S;Watzl, C; Chronic stimulation desensitizes ?2-adrenergic receptor responses in natural killer cells European journal of immunology 2024-09-30 [PMID: 39350450] (Neutralization, ELISA Capture, Human)
• Sugawara S, Hueber B, Woolley G et al. Multiplex interrogation of the NK cell signalome reveals global downregulation of CD16 signaling during lentivirus infection through an IL-18/ADAM17-dependent mechanism PLoS pathogens 9/1/2023 [PMID: 37669308]
• King, LA;Toffoli, EC;Veth, M;Iglesias-Guimarais, V;Slot, MC;Amsen, D;van de Ven, R;Derks, S;Fransen, MF;Tuynman, JB;Riedl, T;Roovers, RC;Adang, AEP;Ruben, JM;Parren, PWHI;de Gruijl, TD;van der Vliet, HJ; A bispecific ?? T-cell engager targeting EGFR activates a potent V?9V?2 T cell-mediated immune response against EGFR-expressing tumors Cancer immunology research 2023-06-27 [PMID: 37368791] (Neutralization, Human)
• R Laufer Bri, A Keren, M Bertolini, Y Ullmann, R Paus, A Gilhar Involvement of ILC1-like innate lymphocytes in human autoimmunity, lessons from alopecia areata Elife, 2023-03-17;12(0):. 2023-03-17 [PMID: 36930216] (Neutralization, Human)
• A Carmena Mo, Y Carpentier, F Lemaître, N Farzam-Kia, S Da Cal, JV Guimond, E Haddad, P Duquette, JM Girard, A Prat, C Larochelle, N Arbour Specific alterations in NKG2D+ T lymphocytes in relapsing-remitting and progressive multiple sclerosis patients Multiple sclerosis and related disorders, 2023-01-26;71(0):104542. 2023-01-26 [PMID: 36716577] (Blocking/Neutralizing, Human)
• CK Yang, CH Huang, CH Hu, JH Fang, TC Chen, YC Lin, CY Lin Immunophenotype and antitumor activity of cytokine-induced killer cells from patients with hepatocellular carcinoma PLoS ONE, 2023-01-04;18(1):e0280023. 2023-01-04 [PMID: 36598909] (Neutralization, Human)
• MC Puzzolo, M Breccia, P Mariglia, G Colafigli, S Pepe, E Scalzulli, E Mariggiò, R Latagliata, A Guarini, R Foà Immunomodulatory Effects of IFNalpha on T and NK Cells in Chronic Myeloid Leukemia Patients in Deep Molecular Response Preparing for Treatment Discontinuation Journal of Clinical Medicine, 2022-09-23;11(19):. 2022-09-23 [PMID: 36233461] (Flow Cytometry, Human)
• R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement Cell Reports, 2022-09-27;40(13):111423. 2022-09-27 [PMID: 36170817] (Neutralization, Human)
• S Belkahla, JM Brualla, A Fayd'herbe, P Falvo, N Allende-Ve, M Constantin, AUH Khan, L Coenon, C Alexia, G Mitola, P Massa, S Orecchioni, F Bertolini, W Mnif, J Hernandez, A Anel, M Villalba The metabolism of cells regulates their sensitivity to NK cells depending on p53 status Scientific Reports, 2022-02-25;12(1):3234. 2022-02-25 [PMID: 35217717] (Neutralization, Human)
• N Allende-Ve, J Marco Brua, P Falvo, C Alexia, M Constantin, AF de Maudave, L Coenon, D Gitenay, G Mitola, P Massa, S Orecchioni, F Bertolini, I Marzo, A Anel, M Villalba Metformin sensitizes leukemic cells to cytotoxic lymphocytes by increasing expression of intercellular adhesion molecule-1 (ICAM-1) Scientific Reports, 2022-01-25;12(1):1341. 2022-01-25 [PMID: 35079096] (Neutralization, Human)
• HY Wu, KX Li, WY Pan, MQ Guo, DZ Qiu, YJ He, YH Li, YX Huang Venetoclax enhances NK cell killing sensitivity of AML cells through the NKG2D/NKG2DL activation pathway International immunopharmacology, 2022-01-06;104(0):108497. 2022-01-06 [PMID: 34999394] (Neutralization, Human)
• D Westhoff S, A Chakravort, M Hayes, W Hammerschm, B Sugden The Epstein-Barr Virus Oncogene EBNA1 Suppresses Natural Killer Cell Responses and Apoptosis Early after Infection of Peripheral B Cells MBio, 2021-11-16;0(0):e0224321. 2021-11-16 [PMID: 34781735] (Neutralization, Human)
• S Petillo, C Capuano, R Molfetta, C Fionda, A Mekhloufi, C Pighi, F Antonangel, A Zingoni, A Soriani, MT Petrucci, R Galandrini, R Paolini, A Santoni, M Cippitelli Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition Cell Death & Disease, 2021-09-04;12(9):836. 2021-09-04 [PMID: 34482362] (Neutralization, Human)
• Samuel M. Duncan, Rupa Nagar, Manuela Damerow, Dmitry V. Yashunsky, Benedetta Buzzi, Andrei V. Nikolaev et al. A Trypanosoma brucei beta 3 glycosyltransferase superfamily gene encodes a beta 1-6 GlcNAc-transferase mediating N-glycan and GPI anchor modification Journal of Biological Chemistry 10/1/2021 [PMID: 34478712]
• X Zhong, H Liao, S Hu, K Luo, H Zhu The diterpenoid adenanthin upregulates the expression of natural killer group 2D receptor ligands in hepatocellular carcinoma cells Molecular and cellular probes, 2021-07-12;59(0):101759. 2021-07-12 [PMID: 34265372] (Flow Cytometry, Human)
• E Seidel, L Dassa, C Schuler, E Oiknine-Dj, DG Wolf, VTK Le-Trillin, O Mandelboim The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing PloS Pathogens, 2021-05-03;17(5):e1008807. 2021-05-03 [PMID: 33939764] (Neutralization, Human)
• Prasad V. Phatarpekar, Brittany L. Overlee, Alexander Leehan, Katelynn M. Wilton, Hyoungjun Ham, Daniel D. Billadeau The septin cytoskeleton regulates natural killer cell lytic granule release Journal of Cell Biology 11/2/2020 [PMID: 32841357]
• M Diab, D Schmiedel, E Seidel, E Bacharach, O Mandelboim Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D Viruses, 2020-07-20;12(7):. 2020-07-20 [PMID: 32698530] (Neutralization, Human)
• S Capellero, J Erriquez, C Melano, G Mesiano, S Genta, A Pisacane, G Mittica, E Ghisoni, M Olivero, MF Di Renzo, M Aglietta, D Sangiolo, G Valabrega Preclinical immunotherapy with Cytokine-Induced Killer lymphocytes against epithelial ovarian cancer Sci Rep, 2020-04-15;10(1):6478. 2020-04-15 [PMID: 32296104] (Neutralization, Human)
• S Sasawatari, Y Okamoto, A Kumanogoh, T Toyofuku Blockade of N-Glycosylation Promotes Antitumor Immune Response of T Cells J. Immunol., 2020-01-22;0(0):. 1/22/2020 [PMID: 31969386]
• Cristina Capuano, Chiara Pighi, Roberta Maggio, Simone Battella, Stefania Morrone, Gabriella Palmieri et al. CD16 pre-ligation by defucosylated tumor-targeting mAb sensitizes human NK cells to gamma c cytokine stimulation via PI3K/mTOR axis Cancer Immunology, Immunotherapy 4/1/2020 [PMID: 31950225]
• E Oh, B Min, Y Li, C Lian, J Hong, GM Park, B Yang, SY Cho, YK Hwang, CO Yun Cryopreserved Human Natural Killer Cells Exhibit Potent Antitumor Efficacy against Orthotopic Pancreatic Cancer through Efficient Tumor-Homing and Cytolytic Ability (Running Title: Cryopreserved NK Cells Exhibit Antitumor Effect) Cancers (Basel), 2019-07-09;11(7):. 2019-07-09 [PMID: 31324057] (Neutralization, Human)
• A Gras Navar, H Espedal, JV Joseph, L Trachsel-M, M Bahador, B Tore Gjert, E Klæboe Kri, A Simonsen, H Miletic, P Øyvind Eng, MA Rahman, M Chekenya Pretreatment of Glioblastoma with Bortezomib Potentiates Natural Killer Cell Cytotoxicity through TRAIL/DR5 Mediated Apoptosis and Prolongs Animal Survival Cancers (Basel), 2019-07-17;11(7):. 2019-07-17 [PMID: 31319548] (Neutralization, Human)
• HG Wirsching, H Zhang, F Szulzewsky, S Arora, P Grandi, PJ Cimino, N Amankulor, JS Campbell, L McFerrin, SS Pattwell, C Ene, A Hicks, M Ball, J Yan, J Zhang, D Kumasaka, RH Pierce, M Weller, M Finer, C Quéva, JC Glorioso, AM Houghton, EC Holland Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma JCI Insight, 2019-07-11;4(13):. 2019-07-11 [PMID: 31292299] (Flow Cytometry, Human, Mouse)
• KM Wilton, BL Overlee, DD Billadeau NKG2D/DAP10 Signaling recruits EVL to the cytotoxic synapse to generate F-actin and promote NK cell cytotoxicity J. Cell. Sci., 2019-07-18;0(0):. 2019-07-18 [PMID: 31235500] (Cytotoxicity Assay, Stimulation, Human, Mouse)
• DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging JCI Insight, 2019-06-11;5(0):. 2019-06-11 [PMID: 31184599] (IHC, Human)
• Xiaoxuan Zhuang, Eric O. Long CD28 Homolog Is a Strong Activator of Natural Killer Cells for Lysis of B7H7+ Tumor Cells Cancer Immunology Research 6/1/2019 [PMID: 31018957]
• CF Amorim, NB Carvalho, JA Neto, SB Santos, MFR Grassi, LP Carvalho, EM Carvalho The Role of NK Cells in the Control of Viral Infection in HTLV-1 Carriers J Immunol Res, 2019-02-28;2019(0):6574828. 2019-02-28 [PMID: 30944834] (Neutralization, Human)
• Erica Giuliani, Maria Giovanna Desimio, Margherita Doria Hexamethylene bisacetamide impairs NK cell-mediated clearance of acute T lymphoblastic leukemia cells and HIV-1-infected T cells that exit viral latency Scientific Reports 2019-03-13 [PMID: 30867508] (Bioassay, Human)
• B Müller-Dur, J Grählert, OP Devine, AN Akbar, C Hess CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age Aging (Albany NY), 2019-01-27;11(2):724-740. 2019-01-27 [PMID: 30686790] (Flow Cytometry, Human)
• J Prévost, J Richard, H Medjahed, A Alexander, J Jones, JC Kappes, C Ochsenbaue, A Finzi Incomplete downregulation of CD4 expression affects HIV-1 Env conformation and ADCC responses J. Virol., 2018-06-13;0(0):. 2018-06-13 [PMID: 29669829] (Neutralization, Human)
• YS Lee, W Heo, J Nam, YH Jeung, J Bae The combination of ionizing radiation and proteasomal inhibition by bortezomib enhances the expression of NKG2D ligands in multiple myeloma cells J. Radiat. Res., 2018-05-01;0(0):. 2018-05-01 [PMID: 29518205] (Neutralization, Human)
• YM Dai, HY Liu, YF Liu, Y Zhang, W He EBV transformation induces overexpression of hMSH2/3/6 on B lymphocytes and enhances ??T cell-mediated cytotoxicity via TCR and NKG2D Immunology, 2018-03-07;0(0):. 2018-03-07 [PMID: 29512904] (Neutralization, Human)
• O Matalon, A Ben-Shmuel, J Kivelevitz, B Sabag, S Fried, N Joseph, E Noy, G Biber, M Barda-Saad Actin retrograde flow controls natural killer cell response by regulating the conformation state of SHP-1 EMBO J., 2018-02-15;0(0):. 2018-02-15 [PMID: 29449322] (Functional Assay, Human)
• S López-Cobo, N Pieper, C Campos-Sil, EM García-Cue, HT Reyburn, A Paschen, M Valés-Góme Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors Oncoimmunology, 2017-11-06;7(2):e1392426. 2017-11-06 [PMID: 29308322] (Bioassay, Human)
• Paul J Brighton, Yojiro Maruyama, Katherine Fishwick, Pavle Vrljicak, Shreeya Tewary, Risa Fujihara et al. Clearance of senescent decidual cells by uterine natural killer cells in cycling human endometrium eLife 2017-12-11 [PMID: 29227245] (Bioassay, Human)
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• JRM Van Audena, J De Waele, E Marcq, J Van Loenho, E Lion, JMJ Van den Be, R Jesenofsky, A Masamune, G Roeyen, P Pauwels, F Lardon, M Peeters, ELJ Smits Interleukin-15 stimulates natural killer cell-mediated killing of both human pancreatic cancer and stellate cells Oncotarget, 2017-01-01;0(0):. 2017-01-01 [PMID: 28615524] (Neutralization, Human)
• X Cai, A Caballero-, MM Gewe, IC Jenkins, CW Drescher, RK Strong, T Spies, V Groh Control of Tumor Initiation by NKG2D Naturally Expressed on Ovarian Cancer Cells Neoplasia, 2017-05-09;19(6):471-482. 2017-05-09 [PMID: 28499126] (Functional Assay, Human)
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• Sangiolo , Dario, Mesiano , Giulia, Gammaitoni , Loretta, Leuci , Valeria, Todorovic , Maja, Giraudo , Lidia, Cammarata , Cristina, Dell'Aglio , Carmine, D'Ambrosio , Lorenzo, Pisacane , Alberto, Sarotto , Ivana, Miano , Sara, Ferrero , Ivana, Carnevale-Schianca , Fabrizio, Pignochino , Ymera, Sassi , Francesc, Bertotti , Andrea, Piacibello , Wanda, Fagioli , Franca, Aglietta , Massimo, Grignani , Giovanni Cytokine-induced killer cells eradicate bone and soft-tissue sarcomas. Cancer Res, 2013-12-19;74(1):119-29. 2013-12-19 [PMID: 24356422] (Neutralization, Human)
• Windheim , Mark, Southcombe , Jennifer, Kremmer , Elisabet, Chaplin , Lucy, Urlaub , Doris, Falk , Christin, Claus , Maren, Mihm , Janine, Braithwaite , Myles, Dennehy , Kevin, Renz , Harald, Sester , Martina, Watzl , Carsten, Burgert , Hans-Ger A unique secreted adenovirus E3 protein binds to the leukocyte common antigen CD45 and modulates leukocyte functions. Proc Natl Acad Sci U S A, 2013-11-11;110(50):E4884-93. 2013-11-11 [PMID: 24218549] (Bioassay, Human)
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• Ho , Liam Poc, Yit , Pui San, Ng , Lee Hui, Linn , Yeh Chin, Zhao , Yi, Sun , Li, Ling , Khoon Li, Chai Koh , Mickey B, Monica Shih , Meng-Chu, Li , Shang, Wang , Xue Ying, Tien , Sim Leng, Goh , Yeow Tee The road to memory: an early rest for the long journey. J Immunol, 2013-11-01;191(11):5603-14. 2013-11-01 [PMID: 24184558] (Neutralization, Human)
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• Wiskott-Aldrich syndrome protein (WASp) and N-WASp are involved in the regulation of NK-cell migration upon NKG2D activation. Eur J Immunol, 2012-08-01;42(8):2142-51. 2012-08-01 [PMID: 22585739] (Functional Assay, Human)
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• Maria Raffaella Zocchi, Silvia Catellani, Paolo Canevali, Sara Tavella, Anna Garuti, Barbara Villaggio et al. High ERp5/ADAM10 expression in lymph node microenvironment and impaired NKG2D ligands recognition in Hodgkin lymphomas Blood 2/9/2012 [PMID: 22167753]
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Review for NKG2D/CD314 Antibody (MAB139) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/28/2022

Summary

• Application: Western Blot
• Sample Tested: Peripheral blood mononuclear cells (PBMCs)
• Species: Human
• Lot: 149810

Bioinformatics

• Gene Symbol: KLRK1