NKG2D/CD314 Antibody (149810) [Unconjugated]

Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by anti-Mouse IgG PE-conjugated Monoclonal Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD8 APC-conjugated Monoclonal Antibody (FAB1509A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or (B) Mouse IgG1 Isotype Control (MAB002) followed by anti-Mouse IgG PE-conjugated Monoclonal Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.

• Reactivity: Hu
• Applications: WB, Flow, B/N, CyTOF-ready, Func
• Clone: 149810
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NKG2D/CD314 Antibody (149810) [Unconjugated] Summary

• Additional Information: Clone 149810 was used by HLDA to establish CD designation
• Immunogen: BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
• Specificity: Detects human NKG2D/CD314 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLRK1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Costimulation of T Cells
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKG2D/CD314 Antibody (149810) [Unconjugated]

• CD314 antigen
• CD314
• D12S2489E
• FLJ17759
• FLJ75772
• Killer cell lectin-like receptor subfamily K member 1
• killer cell lectin-like receptor subfamily K, member 1
• KLR
• KLRK1
• NK cell receptor D
• NKG2-D type II integral membrane protein
• NKG2D
• NKG2-D
• NKG2-D-activating NK receptor
• NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

Background

NKG2D, also known as CD314, is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8⁺ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

1. Li, P. et al. (2001) Nature Immunol. 2:443.
2. Steinle, A. et al. (2001) Immunogenetics 53:279.
3. Cosman, D. et al. (2001) Immunity 14:123.
4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
5. Wu, J. et al. (1999) Science 285:730.
6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
8. Groh, V. et al. (2001) Nature Immunol. 2:255.
9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
10. Diefenbach, A. et al. (2001) Nature 413:165.
11. NKG2D and its Ligands (2002) www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKG2D/CD314 Antibody (MAB139)(83)

Publications using MAB139

• A Carmena Mo, Y Carpentier, F Lemaître, N Farzam-Kia, S Da Cal, JV Guimond, E Haddad, P Duquette, JM Girard, A Prat, C Larochelle, N Arbour Specific alterations in NKG2D+ T lymphocytes in relapsing-remitting and progressive multiple sclerosis patients Multiple sclerosis and related disorders, 2023;71(0):104542. 2023 [PMID: 36716577] (Blocking/Neutralizing, Human)
• MC Puzzolo, M Breccia, P Mariglia, G Colafigli, S Pepe, E Scalzulli, E Mariggiò, R Latagliata, A Guarini, R Foà Immunomodulatory Effects of IFNalpha on T and NK Cells in Chronic Myeloid Leukemia Patients in Deep Molecular Response Preparing for Treatment Discontinuation Journal of Clinical Medicine, 2022;11(19):. 2022 [PMID: 36233461] (Flow Cytometry, Human)
• R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement Cell Reports, 2022;40(13):111423. 2022 [PMID: 36170817] (Neutralization, Human)
• S Belkahla, JM Brualla, A Fayd'herbe, P Falvo, N Allende-Ve, M Constantin, AUH Khan, L Coenon, C Alexia, G Mitola, P Massa, S Orecchioni, F Bertolini, W Mnif, J Hernandez, A Anel, M Villalba The metabolism of cells regulates their sensitivity to NK cells depending on p53 status Scientific Reports, 2022;12(1):3234. 2022 [PMID: 35217717] (Neutralization, Human)
• N Allende-Ve, J Marco Brua, P Falvo, C Alexia, M Constantin, AF de Maudave, L Coenon, D Gitenay, G Mitola, P Massa, S Orecchioni, F Bertolini, I Marzo, A Anel, M Villalba Metformin sensitizes leukemic cells to cytotoxic lymphocytes by increasing expression of intercellular adhesion molecule-1 (ICAM-1) Scientific Reports, 2022;12(1):1341. 2022 [PMID: 35079096] (Neutralization, Human)
• HY Wu, KX Li, WY Pan, MQ Guo, DZ Qiu, YJ He, YH Li, YX Huang Venetoclax enhances NK cell killing sensitivity of AML cells through the NKG2D/NKG2DL activation pathway International immunopharmacology, 2022;104(0):108497. 2022 [PMID: 34999394] (Neutralization, Human)
• D Westhoff S, A Chakravort, M Hayes, W Hammerschm, B Sugden The Epstein-Barr Virus Oncogene EBNA1 Suppresses Natural Killer Cell Responses and Apoptosis Early after Infection of Peripheral B Cells MBio, 2021;0(0):e0224321. 2021 [PMID: 34781735] (Neutralization, Human)
• S Petillo, C Capuano, R Molfetta, C Fionda, A Mekhloufi, C Pighi, F Antonangel, A Zingoni, A Soriani, MT Petrucci, R Galandrini, R Paolini, A Santoni, M Cippitelli Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition Cell Death & Disease, 2021;12(9):836. 2021 [PMID: 34482362] (Neutralization, Human)
• X Zhong, H Liao, S Hu, K Luo, H Zhu The diterpenoid adenanthin upregulates the expression of natural killer group 2D receptor ligands in hepatocellular carcinoma cells Molecular and cellular probes, 2021;59(0):101759. 2021 [PMID: 34265372] (Flow Cytometry, Human)
• E Seidel, L Dassa, C Schuler, E Oiknine-Dj, DG Wolf, VTK Le-Trillin, O Mandelboim The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing PloS Pathogens, 2021;17(5):e1008807. 2021 [PMID: 33939764] (Neutralization, Human)
• M Diab, D Schmiedel, E Seidel, E Bacharach, O Mandelboim Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D Viruses, 2020;12(7):. 2020 [PMID: 32698530] (Neutralization, Human)
• S Capellero, J Erriquez, C Melano, G Mesiano, S Genta, A Pisacane, G Mittica, E Ghisoni, M Olivero, MF Di Renzo, M Aglietta, D Sangiolo, G Valabrega Preclinical immunotherapy with Cytokine-Induced Killer lymphocytes against epithelial ovarian cancer Sci Rep, 2020;10(1):6478. 2020 [PMID: 32296104] (Neutralization, Human)
• E Oh, B Min, Y Li, C Lian, J Hong, GM Park, B Yang, SY Cho, YK Hwang, CO Yun Cryopreserved Human Natural Killer Cells Exhibit Potent Antitumor Efficacy against Orthotopic Pancreatic Cancer through Efficient Tumor-Homing and Cytolytic Ability (Running Title: Cryopreserved NK Cells Exhibit Antitumor Effect) Cancers (Basel), 2019;11(7):. 2019 [PMID: 31324057] (Neutralization, Human)
• A Gras Navar, H Espedal, JV Joseph, L Trachsel-M, M Bahador, B Tore Gjert, E Klæboe Kri, A Simonsen, H Miletic, P Øyvind Eng, MA Rahman, M Chekenya Pretreatment of Glioblastoma with Bortezomib Potentiates Natural Killer Cell Cytotoxicity through TRAIL/DR5 Mediated Apoptosis and Prolongs Animal Survival Cancers (Basel), 2019;11(7):. 2019 [PMID: 31319548] (Neutralization, Human)
• HG Wirsching, H Zhang, F Szulzewsky, S Arora, P Grandi, PJ Cimino, N Amankulor, JS Campbell, L McFerrin, SS Pattwell, C Ene, A Hicks, M Ball, J Yan, J Zhang, D Kumasaka, RH Pierce, M Weller, M Finer, C Quéva, JC Glorioso, AM Houghton, EC Holland Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma JCI Insight, 2019;4(13):. 2019 [PMID: 31292299] (Flow Cytometry, Human)
• KM Wilton, BL Overlee, DD Billadeau NKG2D/DAP10 Signaling recruits EVL to the cytotoxic synapse to generate F-actin and promote NK cell cytotoxicity J. Cell. Sci., 2019;0(0):. 2019 [PMID: 31235500] (Stimulation, Human)
• DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging JCI Insight, 2019;5(0):. 2019 [PMID: 31184599] (IHC, Human)
• CF Amorim, NB Carvalho, JA Neto, SB Santos, MFR Grassi, LP Carvalho, EM Carvalho The Role of NK Cells in the Control of Viral Infection in HTLV-1 Carriers J Immunol Res, 2019;2019(0):6574828. 2019 [PMID: 30944834] (Neutralization, Human)
• B Müller-Dur, J Grählert, OP Devine, AN Akbar, C Hess CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age Aging (Albany NY), 2019;11(2):724-740. 2019 [PMID: 30686790] (Flow Cytometry, Human)
• J Prévost, J Richard, H Medjahed, A Alexander, J Jones, JC Kappes, C Ochsenbaue, A Finzi Incomplete downregulation of CD4 expression affects HIV-1 Env conformation and ADCC responses J. Virol., 2018;0(0):. 2018 [PMID: 29669829] (Neutralization, Human)
• YS Lee, W Heo, J Nam, YH Jeung, J Bae The combination of ionizing radiation and proteasomal inhibition by bortezomib enhances the expression of NKG2D ligands in multiple myeloma cells J. Radiat. Res., 2018;0(0):. 2018 [PMID: 29518205] (Neutralization, Human)
• YM Dai, HY Liu, YF Liu, Y Zhang, W He EBV transformation induces overexpression of hMSH2/3/6 on B lymphocytes and enhances ??T cell-mediated cytotoxicity via TCR and NKG2D Immunology, 2018;0(0):. 2018 [PMID: 29512904] (Neutralization, Human)
• O Matalon, A Ben-Shmuel, J Kivelevitz, B Sabag, S Fried, N Joseph, E Noy, G Biber, M Barda-Saad Actin retrograde flow controls natural killer cell response by regulating the conformation state of SHP-1 EMBO J., 2018;0(0):. 2018 [PMID: 29449322] (Functional Assay, Human)
• S López-Cobo, N Pieper, C Campos-Sil, EM García-Cue, HT Reyburn, A Paschen, M Valés-Góme Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors Oncoimmunology, 2018;7(2):e1392426. 2018 [PMID: 29308322] (Bioassay, Human)
• Y Charpak-Am, T Kubsch, E Seidel, E Oiknine-Dj, N Cavaletto, R Yamin, D Schmiedel, D Wolf, G Gribaudo, M Messerle, L Cicin-Sain, O Mandelboim Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20 Sci Rep, 2017;7(1):8661. 2017 [PMID: 28819195] (Neutralization, Human)
• S Santaguida, A Richardson, DR Iyer, O M'Saad, L Zasadil, KA Knouse, YL Wong, N Rhind, A Desai, A Amon Chromosome Mis-segregation Generates Cell-Cycle-Arrested Cells with Complex Karyotypes that Are Eliminated by the Immune System Dev. Cell, 2017;41(6):638-651.e5. 2017 [PMID: 28633018] (Neutralization, Human)
• JRM Van Audena, J De Waele, E Marcq, J Van Loenho, E Lion, JMJ Van den Be, R Jesenofsky, A Masamune, G Roeyen, P Pauwels, F Lardon, M Peeters, ELJ Smits Interleukin-15 stimulates natural killer cell-mediated killing of both human pancreatic cancer and stellate cells Oncotarget, 2017;0(0):. 2017 [PMID: 28615524] (Neutralization, Human)
• X Cai, A Caballero-, MM Gewe, IC Jenkins, CW Drescher, RK Strong, T Spies, V Groh Control of Tumor Initiation by NKG2D Naturally Expressed on Ovarian Cancer Cells Neoplasia, 2017;19(6):471-482. 2017 [PMID: 28499126] (Functional Assay, Human)
• Z Djaoud, LA Guethlein, A Horowitz, T Azzi, N Nemat-Gorg, D Olive, D Nadal, PJ Norman, C Münz, P Parham Two alternate strategies for innate immunity to Epstein-Barr virus: One using NK cells and the other NK cells and ?? T cells J. Exp. Med., 2017;0(0):. 2017 [PMID: 28468758] (Neutralization, Human)
• F Lin, C Dai, X Ge, W Tang, Y Lin, Y Wang, J Li Prognostic significance and functional implication of immune activating receptor NKG2D in gastric cancer Biochem. Biophys. Res. Commun., 2017;0(0):. 2017 [PMID: 28433635] (Neutralization, Human)
• H Jensen, SY Chen, L Folkersen, GP Nolan, LL Lanier EBI3 regulates the NK cell response to mouse cytomegalovirus infection Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28143936] (Functional Assay, Human)
• A functional polymorphism in the NKG2D gene modulates NK-cell cytotoxicity and is associated with susceptibility to Human Papilloma Virus-related cancers Sci Rep, 2016;6(0):39231. 2016 [PMID: 27995954] (Neutralization, Human)
• C Niu, H Jin, M Li, S Zhu, L Zhou, F Jin, Y Zhou, D Xu, J Xu, L Zhao, S Hao, W Li, J Cui Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and ?? T cell-mediated lysis in multiple myeloma Oncotarget, 2017;8(4):5954-5964. 2017 [PMID: 27992381] (Neutralization, Human)
• NK cell function is markedly impaired in patients with chronic lymphocytic leukaemia but is preserved in patients with small lymphocytic lymphoma Oncotarget, 2016;0(0):. 2016 [PMID: 27655680] (Neutralization, Human)
• M Ke, H Wang, Y Zhou, J Li, Y Liu, M Zhang, J Dou, T Xi, B Shen, C Zhou SEP enhanced the antitumor activity of 5-fluorouracil by up-regulating NKG2D/MICA and reversed immune suppression via inhibiting ROS and caspase-3 in mice Oncotarget, 2016;7(31):49509-49526. 2016 [PMID: 27385218] (Neutralization, Human)
• Davis Z, Sowrirajan B, Cogswell A, Ward J, Planelles V, Barker E CD155 on HIV-Infected Cells Is Not Modulated by HIV-1 Vpu and Nef but Synergizes with NKG2D Ligands to Trigger NK Cell Lysis of Autologous Primary HIV-Infected Cells. AIDS Res Hum Retroviruses, 0;33(2):93-100. 0 [PMID: 27296670] (Neutralization, Human)
• Kruse V, Hamann C, Monecke S, Cyganek L, Elsner L, Hubscher D, Walter L, Streckfuss-Bomeke K, Guan K, Dressel R Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1. PLoS ONE, 2015;10(5):e0125544. 2015 [PMID: 25950680] (Flow Cytometry, Human)
• Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer E, Cerwenka A, Steinle A, Koehl U, Koch J Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids. BMC Cancer, 2015;15(0):351. 2015 [PMID: 25933805] (Neutralization, Human)
• Frishman-Levy L, Shemesh A, Bar-Sinai A, Ma C, Ni Z, Frenkel S, Muench V, Bruckmueller H, Vokuhl C, Debatin K, Eckert C, Stanulla M, Schrappe M, Campbell K, Loewenthal R, Schewe D, Hochman J, Meyer L, Kaufman D, Cario G, Porgador A, Izraeli S Central nervous system acute lymphoblastic leukemia: role of natural killer cells. Blood, 2015;125(22):3420-31. 2015 [PMID: 25896649] (Neutralization, Human)
• Karimi M, Aguilar O, Zou B, Bachmann M, Carlyle J, Baldwin C, Kambayashi T A truncated human NKG2D splice isoform negatively regulates NKG2D-mediated function. J Immunol, 2014;193(6):2764-71. 2014 [PMID: 25092887] (Flow Cytometry, Human)
• Celis-Gutierrez J, Boyron M, Walzer T, Pandolfi P, Jonjic S, Olive D, Dalod M, Vivier E, Nunes J Dok1 and Dok2 proteins regulate natural killer cell development and function. EMBO J, 2014;33(17):1928-40. 2014 [PMID: 24963146] (Bioassay, Human)
• Molfetta R, Quatrini L, Capuano C, Gasparrini F, Zitti B, Zingoni A, Galandrini R, Santoni A, Paolini R c-Cbl regulates MICA- but not ULBP2-induced NKG2D down-modulation in human NK cells. Eur J Immunol, 2014;44(9):2761-70. 2014 [PMID: 24846123] (ICC, Human)
• Serrano-Pertierra E, Cernuda-Morollon E, Brdicka T, Hooejsi V, Lopez-Larrea C L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration. J Leukoc Biol, 2014;96(3):437-45. 2014 [PMID: 24803550] (Neutralization, Human)
• Awad A, Yassine H, Barrier M, Vorng H, Marquillies P, Tsicopoulos A, Duez C Natural killer cells induce eosinophil activation and apoptosis. PLoS ONE, 2014;9(4):e94492. 2014 [PMID: 24727794] (Neutralization, Human)
• Sangiolo , Dario, Mesiano , Giulia, Gammaitoni , Loretta, Leuci , Valeria, Todorovic , Maja, Giraudo , Lidia, Cammarata , Cristina, Dell'Aglio , Carmine, D'Ambrosio , Lorenzo, Pisacane , Alberto, Sarotto , Ivana, Miano , Sara, Ferrero , Ivana, Carnevale-Schianca , Fabrizio, Pignochino , Ymera, Sassi , Francesc, Bertotti , Andrea, Piacibello , Wanda, Fagioli , Franca, Aglietta , Massimo, Grignani , Giovanni Cytokine-induced killer cells eradicate bone and soft-tissue sarcomas. Cancer Res, 2014;74(1):119-29. 2014 [PMID: 24356422] (Neutralization, Human)
• Windheim , Mark, Southcombe , Jennifer, Kremmer , Elisabet, Chaplin , Lucy, Urlaub , Doris, Falk , Christin, Claus , Maren, Mihm , Janine, Braithwaite , Myles, Dennehy , Kevin, Renz , Harald, Sester , Martina, Watzl , Carsten, Burgert , Hans-Ger A unique secreted adenovirus E3 protein binds to the leukocyte common antigen CD45 and modulates leukocyte functions. Proc Natl Acad Sci U S A, 2013;110(50):E4884-93. 2013 [PMID: 24218549] (Bioassay, Human)
• Leung W, Vong Q, Lin W, Janke L, Chen T, Leung W Modulation of NKG2D ligand expression and metastasis in tumors by spironolactone via RXRgamma activation. J Exp Med, 2013;210(12):2675-92. 2013 [PMID: 24190430] (Neutralization, Human)
• Ho , Liam Poc, Yit , Pui San, Ng , Lee Hui, Linn , Yeh Chin, Zhao , Yi, Sun , Li, Ling , Khoon Li, Chai Koh , Mickey B, Monica Shih , Meng-Chu, Li , Shang, Wang , Xue Ying, Tien , Sim Leng, Goh , Yeow Tee The road to memory: an early rest for the long journey. J Immunol, 2013;191(11):5603-14. 2013 [PMID: 24184558] (Neutralization, Human)
• Hosomi S, Chen Z, Baker K, Chen L, Huang Y, Olszak T, Zeissig S, Wang J, Mandelboim O, Beauchemin N, Lanier L, Blumberg R CEACAM1 on activated NK cells inhibits NKG2D-mediated cytolytic function and signaling. Eur J Immunol, 2013;43(9):2473-83. 2013 [PMID: 23696226] (Neutralization, Human)
• Lim S, Kim T, Lee J, Sonn C, Kim K, Kim J, Choi J, Choi I, Yun C, Kim J, Yee C, Kumar V, Lee K Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy. Cancer Res, 2013;73(8):2598-607. 2013 [PMID: 23580577] (Neutralization, Human)
• Di Carlo E, Bocca P, Emionite L, Cilli M, Cipollone G, Morandi F, Raffaghello L, Pistoia V, Prigione I Mechanisms of the antitumor activity of human Vgamma9Vdelta2 T cells in combination with zoledronic acid in a preclinical model of neuroblastoma. Mol Ther, 2013;21(5):1034-43. 2013 [PMID: 23481325] (Bioassay, Human)
• Ham , Hyoungju, Guerrier , Sabrice, Kim , JungJin, Schoon , Renee A, Anderson , Erik L, Hamann , Michael, Lou , Zhenkun, Billadeau , Daniel D Dedicator of cytokinesis 8 interacts with talin and Wiskott-Aldrich syndrome protein to regulate NK cell cytotoxicity. J Immunol, 2013;190(7):3661-9. 2013 [PMID: 23455509] (Functional Assay, Human)
• Donia M, Ellebaek E, Andersen M, Straten P, Svane I Analysis of Vdelta1 T cells in clinical grade melanoma-infiltrating lymphocytes. Oncoimmunology, 2012;1(8):1297-1304. 2012 [PMID: 23243593] (Blocking, Human)
• Brown A, Dobbie I, Alakoskela J, Davis I, Davis D Super-resolution imaging of remodeled synaptic actin reveals different synergies between NK cell receptors and integrins. Blood, 2012;120(18):3729-40. 2012 [PMID: 22966166] (Bioassay, Human)
• Tsukerman P, Stern-Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, Yamin R, Vitenshtein A, Stanietsky N, Bar-Mag T, Lankry D, Mandelboim O MiR-10b Downregulates the Stress-Induced Cell Surface Molecule MICB, a Critical Ligand for Cancer Cell Recognition by Natural Killer Cells. Cancer Res, 2012;72(21):5463-72. 2012 [PMID: 22915757] (Flow Cytometry, Human)
• Morandi F, Prigione I, Airoldi I Human TCR gamma delta+ T cells represent a novel target for IL-27 activity. Eur. J. Immunol., 2012;42(6):1547-52. 2012 [PMID: 22678908] (Neutralization, Human)
• Wiskott-Aldrich syndrome protein (WASp) and N-WASp are involved in the regulation of NK-cell migration upon NKG2D activation. Eur J Immunol, 2012;42(8):2142-51. 2012 [PMID: 22585739] (Functional Assay, Human)
• Glassner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner. Lab. Invest., 2012;92(7):967-77. 2012 [PMID: 22449797] (Neutralization, Human)
• Nielsen N, Odum N, Urso B, Lanier LL, Spee P Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A. PLoS ONE, 2012;7(2):e31959. 2012 [PMID: 22384114] (Functional Assay, Human)
• Andresen L, Skovbakke SL, Persson G, Hagemann-Jensen M, Hansen KA, Jensen H, Skov S 2-deoxy D-glucose prevents cell surface expression of NKG2D ligands through inhibition of N-linked glycosylation. J. Immunol., 2012;188(4):1847-55. 2012 [PMID: 22227571] (Neutralization, Human)
• Heinemann A, Zhao F, Pechlivanis S, Eberle J, Steinle A, Diederichs S, Schadendorf D, Paschen A Tumor suppressive microRNAs miR-34a/c control cancer cell expression of ULBP2, a stress-induced ligand of the natural killer cell receptor NKG2D. Cancer Res., 2012;72(2):460-71. 2012 [PMID: 22102694] (Flow Cytometry, Human)
• Ardolino M, Zingoni A, Cerboni C, Cecere F, Soriani A, Iannitto ML, Santoni A DNAM-1 ligand expression on Ag-stimulated T lymphocytes is mediated by ROS-dependent activation of DNA-damage response: relevance for NK-T cell interaction. Blood, 2011;117(18):4778-86. 2011 [PMID: 21406724] (Flow Cytometry, Human)
• Inoue T, Tashiro Y, Takeuchi M Potent anti-tumor killing activity of the multifunctional Treg cell line HOZOT against human tumors with diverse origins. Int. J. Oncol., 2011;38(5):1299-306. 2011 [PMID: 21373756] (Neutralization, Human)
• Qin H, Lee IF, Panagiotopoulos C, Wang X, Chu AD, Utz PJ, Priatel JJ, Tan R Natural killer cells from children with type 1 diabetes have defects in NKG2D-dependent function and signaling. Diabetes, 2011;60(3):857-66. 2011 [PMID: 21270236] (Functional Assay, Human)
• Angelini DF, Zambello R, Galandrini R, Diamantini A, Placido R, Micucci F, Poccia F, Semenzato G, Borsellino G, Santoni A, Battistini L NKG2A inhibits NKG2C effector functions of gammadelta T cells: implications in health and disease. J. Leukoc. Biol., 2011;89(1):75-84. 2011 [PMID: 20952657] (Functional Assay, Human)
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Review for NKG2D/CD314 Antibody (MAB139) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Samuel Jones WB Human 01/28/2022

Summary

• Application: Western Blot
• Sample Tested: Peripheral blood mononuclear cells (PBMCs)
• Species: Human
• Lot: 149810

Bioinformatics

• Gene Symbol: KLRK1