LAMP-1/CD107a: Novus Biologicals

NB120-19294

LAMP-1/CD107a Antibody - BSA Free

(2 Reviews)

(39 Publications)

100 μg

NB120-19294

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Lamp1 (red) in HeLa cells.

LAMP-1-CD107a-Antibody-Immunocytochemistry-Immunofluorescence-NB120-19294-img0007.jpg

Autophagy associated protein immunoreactivity in HIV-infected brain tissue. (A) Representative images from five randomly selected fields of cells each examined in duplicate frontal lobe white matter sections for the indicated subject groups. The indicated proteins were labeled red & microglia with the cell-type-specific marker Iba1 (green). Blue staining indicates cell nuclei. Arrow heads indicate examples of higher Iba1 immunoreactivity whereas arrows indicate more focal (punctal) vs. diffuse (filamentous) patterns of autophagy associated protein expression. Scale bar = 10 μm. (B) Quantification of relative Iba1 immunoreactivity from (A). F(3,20) = 6.450, p = 0.0031; ∗p < 0.05 when compared to all other subject groups. Error bars show the SEM for the average values of 2–6 regions from each subject group across the six autophagy associated proteins examined. (C) Quantification of the indicated autophagy associated protein relative immunoreactivity from (A). Beclin 1: F(3,12) = 11.29, p = 0.0008; LC3B: F(3,12) = 1.994, p = 0.1687; APG7/ATG7: F(3,12) = 84.20, p = < 0.0001; ATG5: F(3,12) = 6.218, p = 0.0086; p62/SQSTM1: F(3,12) = 87.04, p = < 0.0001; LAMP1: F(3,12) = 8.317, p = 0.0029. ∗p < 0.05 when compared to HIV-negative; #p < 0.05 when compared to HIV-positive; & Ωp < 0.05 when compared to HIV-positive/NCI subjects. Error bars show the SEM for four regions from each subject group. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fmicb.2015.00653/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Differences in neuronal autophagy & dendrite varicosity following HIV-1 Tat protein & morphine treatment. (A) Representative images of neurons transfected with a fluorescent reporter plasmid to monitor autophagic flux at 8 h following the indicated treatments. GFP (green) & GFP + mRFP (yellow) fluorescence are observed prior to the fusion of autophagosomes with lysosomes whereas only mRFP (red) fluorescence is present in post-fusion autolysosomes. DIC, differential interference contrast microscopy image. DAPI (blue) staining indicates cell nuclei. (B) Quantification of autolysosomes (red puncta) from (A). F(3,13) = 8.756, p = 0.0019; ∗p < 0.05 when compared to all other groups. (C) Western blotting analysis of the indicated autophagy associated protein levels at 24 h following the indicated treatments. GAPDH was used as a loading control. Blots are representative of three independent experiments. (D) Quantification of dendrite beading from (A). F(3,77) = 6.429, p = 0.0006; ∗p < 0.05 when compared to control cells. Error bars show the SEM. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fmicb.2015.00653/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - EsxG-EsxH alters phagosomal GAL3, ubiquitin, & LAMP1. (A, C, & E) IF images of GAL3 (A), ubiquitin (FK2 antibody) (C), & LAMP1 (E) in BMDMs that were infected with DsRed-expressing H37Rv (WT) or the delta esxH mutant for 3 h. Images are maximum-intensity projections. Scale bar, 10 μm. Boxed areas in the merged image are shown in higher magnification in the rightmost panel. Mtb, M. tuberculosis. (B, D, & F) Automated image analysis was used to quantify the MFI of GAL3 (B), ubiquitin (D), & LAMP1 (F) colocalized with individual bacilli from 5 fields of a 12-mm coverslip. Data are means ± SEM from one representative experiment from three (A, B, E, & F) or two (C & D) independent experiments. ****, P ≤ 0.0001, Student's t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30482832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Characterization of selected mutant protein glycosylation status or localization.(A) Two independent, representative experiments to analyze the glycosylation status of GFP-NPC1 wild type & GFP-NPC1 P251C/L929C, as determined by immunoblot of indicated samples with anti-GFP antibodies after 6% SDS PAGE; samples were incubated at 70°C in SDS PAGE sample buffer for 10 min prior to loading. Molecular weight markers are shown at left in kilodaltons here & in all subsequent gels shown. (B), Localization of NPC1 P251/L929C/P249K/P259K in NPC1−/− HeLa cells as in Figure 1C. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32410728), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - NPC1 delta loop mutant cannot rescue cholesterol export from lysosomes.(A) Cholesterol-cross-linked peptides (Hulce et al., 2013) are highlighted in red for two orientations of the crystal structure of N-terminal domain- & first transmembrane domain-deleted NPC1 (PDBID: 5u74). The disordered cytoplasmic loop residues 800–814 are shown as a blue dotted line. (B) Confocal immunofluorescence microscopy analysis of the localization of mouse NPC1-delta 807–811, NPC1-807-811Ala & LAMP1 proteins in HeLa cells (bar, 20 μm). White boxes in images indicate regions of cells enlarged in the insets shown at the lower right of each image. (C) Confocal immunofluorescence microscopy of cholesterol accumulation rescue. NPC1−/− HeLa cells were transfected with GFP-tagged mouse NPC1-delta 807–811 or mouse NPC1-807-811Ala plasmids for 48 h & assayed for cholesterol accumulation rescue as in Figure 1 (bar, 20 μm). (D) Quantitation of cholesterol accumulation rescue using flow cytometry. GFP-positive cells with similar expression levels were analyzed: 2480 NPC1; 427 NPC1-delta 807–811; 764 NPC1-807-811Ala; LAMP1 expressing control, 1753 cells counted. Shown are the normalized data from mean fluorescence intensity flow cytometry values. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32410728), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Cathepsin D (CathD) & B (CathB) expression & localization in primary neurons. A. Western blot analyses of CathD & CathB protein levels after transfection. Primary cortical neurons were transfected with 23QHtt, 23QHtt plus CathD, 23QHtt plus CathB, 145QmHtt, 145QmHtt plus CathD & 145QmHtt plus CathB constructs. Western blot analyses were performed with anti-CathD & anti-CathB antibodies. beta -actin western blots were used as loading controls. Relative expression levels were quantified by band intensity. Positions of molecular weight markers were indicated. Quantification of the western blots are shown in the bar graphs. *p < 0.05 compared to without cathepsin transfection. B. Analyses of CathD & CathB enzymatic activities after transfection. Primary cortical neurons were transfected with CathD, CathB & as described in A. CathD & CathB activities were assayed by CathD or CathB activity assay kit. *p < 0.05 compared to without cathepsin transfection. C & D. Colocalization of transfected CathD (C) & CathB (D) with LAMP-1. CathD & CathB transfected neurons were examined by co-immunostaining of LAMP-1 & CathD or CathB. Yellow colored cytoplasmic spots are indicative of co-localization of transfected cathepsins & LAMP-1. Scale bar = 10 micron. Image collected & cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/1750-1326-6-37), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: LAMP-1/CD107a Antibody [NB120-19294] - Effects on autophagic activity & dendritic length of neurons exposed to supernatant from HIV-1-infected microglia in combination with morphine. (A) Representative images of neurons with the indicated treatments. Sup, supernatant from uninfected [HIV(-)] & HIV-1-infected [HIV(+)] microglia. Cells were immunolabeled with antibodies to the autophagic activity marker p62/SQSTM1 (red) & the neuronal cell-type-specific marker MAP2 (green). DAPI (blue) staining indicates cell nuclei. (B) Quantification of p62/SQSTM1 immunoreactivity from (A). Data are presented as the percentage of control cells which was set at 100; F(5,24) = 5.882, p = 0.0011; ∗p < 0.05 when compared to HIV(+)Sup + morphine treatment. (C) Western blotting analysis of p62/SQSTM1 & LAMP1 expression levels for the indicated treatments. GAPDH was used as a loading control. Blots are representative of three independent experiments. (D) Measurement of dendrite length from (A). F(5,24) = 26.15, p = < 0.0001; Φp < 0.05 when compared to morphine; Ψp < 0.05 when compared to HIV(-)Sup; Ωp < 0.05 when compared to HIV(-)Sup + morphine; #p < 0.05 when compared to HIV(+)Sup; & ∗p < 0.05 when compared to HIV(+)Sup + morphine treatment. Error bars show the SEM for five randomly selected fields totaling at least 100 cells from each group. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fmicb.2015.00653/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - NPC1 Disulfide bond-locked MLD & CTD fails to rescue cholesterol export from lysosomes.(A) Partial NPC1 structure; inset, close-up view of the MLD/CTD interface. The amino acid residues mutated to cysteines for disulfide bond formation are shown & highlighted in red. (B) Confocal immunofluorescence microscopy analysis of mouse NPC1-A521C/K1013C & LAMP1 proteins in HeLa cells (bar, 20 μm). White boxes in images indicate regions of cells enlarged in the insets shown at the lower right of each image. (C) Confocal immunofluorescence microscopy of cholesterol accumulation rescue for NPC1-A521C or mouse NPC1-A521C/K1013C. (D) Flow cytometry of the rescue experiment analyzed in (C). GFP-positive cells with similar expression levels were analyzed: 17746 NPC1-/- cells; 1315 NPC1 wild type; 1137 NPC1-A521C/K1013C cells; 837 NPC1-A521C cells; cell numbers were normalized for comparison.Extracted ion chromatograms from LC-MS analysis of proteolyzed A521C/K1013C NPC1.Protein was carbamidomethylated in the presence or absence of reducing agent prior to deglycosylation & proteolysis. In both samples, blue traces represent m/z = 596.2818 (corresponding to the NPC1 peptide APCSLNDTSLL carbamidomethylated at the engineered cysteine A521C & deamidated at the N524 glycosylation site). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32410728), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB120-19294AF350
Alexa Fluor 405	NB120-19294AF405
Alexa Fluor 488	NB120-19294AF488
Alexa Fluor 532	NB120-19294AF532
Alexa Fluor 594	NB120-19294AF594
Alexa Fluor 647	NB120-19294AF647
Alexa Fluor 700	NB120-19294AF700
Alexa Fluor 750	NB120-19294AF750
Alexa Fluor Plus 405	NB120-19294AFP405
Alexa Fluor Plus 488	NB120-19294AFP488
Alexa Fluor Plus 555	NB120-19294AFP555
Alexa Fluor Plus 594	NB120-19294AFP594
Alexa Fluor Plus 647	NB120-19294AFP647
Alexa Fluor Plus 680	NB120-19294AFP680
Allophycocyanin	NB120-19294APC
Biotin	NB120-19294B
CoraFluor 1	NB120-19294CL1
DyLight 350	NB120-19294UV
DyLight 405	NB120-19294V
DyLight 488	NB120-19294G
DyLight 550	NB120-19294R
DyLight 594	NB120-19294DL594
DyLight 650	NB120-19294C
DyLight 680	NB120-19294FR
DyLight 755	NB120-19294IR
FITC	NB120-19294F
HRP	NB120-19294H
Janelia Fluor 525	NB120-19294JF525
Janelia Fluor 549	NB120-19294JF549
Janelia Fluor 646	NB120-19294JF646
Janelia Fluor 669	NB120-19294JF669
PE	NB120-19294PE
PerCP	NB120-19294PCP
mFluor Violet 450 SE	NB120-19294MFV450
mFluor Violet 500 SE	NB120-19294MFV500
mFluor Violet 610 SE	NB120-19294MFV610

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NBP2-52721

LAMP-1/CD107a Antibody (5E7) - BSA Free

(2 Reviews)

(7 Publications)

0.1 mg

NBP2-52721

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-LAMP (5E7) at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - MCF-7 human breast cancer cell line. Secondary antibody goat anti-mouse IgG Fc Dy680 (NBP1-72880). Image from verified customer review.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-52721AF350
Alexa Fluor 405	NBP2-52721AF405
Alexa Fluor 488	NBP2-52721AF488
Alexa Fluor 532	NBP2-52721AF532
Alexa Fluor 594	NBP2-52721AF594
Alexa Fluor 647	NBP2-52721AF647
Alexa Fluor 700	NBP2-52721AF700
Alexa Fluor 750	NBP2-52721AF750
Alexa Fluor Plus 405	NBP2-52721AFP405
Alexa Fluor Plus 488	NBP2-52721AFP488
Alexa Fluor Plus 555	NBP2-52721AFP555
Alexa Fluor Plus 594	NBP2-52721AFP594
Alexa Fluor Plus 647	NBP2-52721AFP647
Alexa Fluor Plus 680	NBP2-52721AFP680
Allophycocyanin	NBP2-52721APC
Biotin	NBP2-52721B
CoraFluor 1	NBP2-52721CL1
DyLight 350	NBP2-52721UV
DyLight 405	NBP2-52721V
DyLight 488	NBP2-52721G
DyLight 550	NBP2-52721R
DyLight 594	NBP2-52721DL594
DyLight 650	NBP2-52721C
DyLight 680	NBP2-52721FR
DyLight 755	NBP2-52721IR
FITC	NBP2-52721F
HRP	NBP2-52721H
Janelia Fluor 525	NBP2-52721JF525
Janelia Fluor 549	NBP2-52721JF549
Janelia Fluor 646	NBP2-52721JF646
Janelia Fluor 669	NBP2-52721JF669
PE	NBP2-52721PE
PerCP	NBP2-52721PCP
mFluor Violet 450 SE	NBP2-52721MFV450
mFluor Violet 500 SE	NBP2-52721MFV500
mFluor Violet 610 SE	NBP2-52721MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-52721SS
Azide and BSA Free	NBP2-80824

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AF4320

LAMP-1/CD107a Antibody [Unconjugated]

(2 Reviews)

(18 Publications)

100 μg

AF4320

HAF017). A specific band was detected for LAMP-1/CD107a at approximately 100-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse small intestine tissue and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse LAMP-1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for LAMP-1/CD107a at approximately 100-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse small intestine tissue and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse LAMP-1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LAMP-1/CD107a at approximately 100-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox">

RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320, filled histogram) or control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

LAMP1/CD107a was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF4320U-100UG
Alexa Fluor 405	AF4320V-100UG
Alexa Fluor 488	AF4320G-100UG
Alexa Fluor 532	AF4320X-100UG
Alexa Fluor 594	AF4320T-100UG
Alexa Fluor 647	AF4320R-100UG
Alexa Fluor 700	AF4320N-100UG
Alexa Fluor 750	AF4320S-100UG
Alexa Fluor Plus 405	AF4320AFP405-100UG
Alexa Fluor Plus 488	AF4320AFP488-100UG
Alexa Fluor Plus 555	AF4320AFP555-100UG
Alexa Fluor Plus 594	AF4320AFP594-100UG
Alexa Fluor Plus 647	AF4320AFP647-100UG
Alexa Fluor Plus 680	AF4320AFP680-100UG
Allophycocyanin	AF4320A
Biotin	AF4320B
CoraFluor 1	AF4320CL1
DyLight 350	AF4320D
DyLight 405	AF4320E
DyLight 488	AF4320K
DyLight 550	AF4320L
DyLight 594	AF4320M
DyLight 650	AF4320W
DyLight 680	AF4320Y
DyLight 755	AF4320Z
FITC	AF4320F
HRP	AF4320H
Janelia Fluor 525	AF4320JF525
Janelia Fluor 549	AF4320I
Janelia Fluor 646	AF4320J
Janelia Fluor 669	AF4320JF669
PE	AF4320P
PerCP	AF4320C
mFluor Violet 450 SE	AF4320MFV450
mFluor Violet 500 SE	AF4320MFV500
mFluor Violet 610 SE	AF4320MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF4320-SP

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NBP2-25183

LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

(1 Publication)

0.1 mg

NBP2-25183

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - IHC staining of LAMP-1 [H4A3] catalog number NBP2-25183 in mouse liver. Cytoplasmic and membrane staining was observed in tubule cells and glomeruli.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on U-87 cells with LAMP-1/CD107a Antibody (H4A3) NBP2-25183PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Human kidney.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on A549 cells with LAMP-1/CD107a antibody (H4A3) NBP2-25183AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Surface staining of human peripheral blood cells with anti-CD107a (H4A3) PE.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on THP-1 cells with LAMP-1/CD107a antibody (H4A3) NBP2-25183PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) azide free, GAM-APC.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) APC.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) Alexa Fluor (R) 488.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Analysis of PHA-activated PBMC with anti-CD107a (H4A3) PerCP-CyTM5.5.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on A431 cells with LAMP-1/CD107a Antibody [H4A3] NBP2-25183AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

LAMP-1/CD107a (H4A3) was detected in immersion fixed A431 human skin carcinoma cell line using Mouse anti- LAMP-1/CD107a (H4A3) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP2-25183AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

LAMP-1/CD107a (H4A3) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti-LAMP-1/CD107a (H4A3) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 488 (Catalog # NBP2-25183AF488) (green) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

An intracellular stain was performed on U-2 OS human osteosarcoma cell line with Mouse anti-LAMP-1/CD107a (H4A3) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 488 (Catalog # NBP2-25183AF488, blue histogram) or matched control antibody (orange histogram) at 5 µg/mL for 30 minutes at RT.

An intracellular stain was performed on A431 human skin carcinoma cell line using Mouse anti-LAMP-1/CD107a (H4A3) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP2-25183AF647, blue histogram) or matched control antibody (orange histogram) at 2.5 µg/mL for 30 minutes at RT.

LAMP-1/CD107a (H4A3) was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse anti-LAMP-1/CD107a (H4A3) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP2-25183AF647) (light blue) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

LAMP-1/CD107a (H4A3) was detected in immersion fixed NIH-3T3 Mouse fibroblast cell line using Mouse anti-LAMP-1/CD107a (H4A3) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP2-25183AF647) (light blue) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu, Pm

Species Glossary

Reactivity: Hu, Mu, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25183AF350
Alexa Fluor 405	NBP2-25183AF405
Alexa Fluor 488	NBP2-25183AF488
Alexa Fluor 532	NBP2-25183AF532
Alexa Fluor 594	NBP2-25183AF594
Alexa Fluor 647	NBP2-25183AF647
Alexa Fluor 700	NBP2-25183AF700
Alexa Fluor 750	NBP2-25183AF750
Alexa Fluor Plus 405	NBP2-25183AFP405
Alexa Fluor Plus 488	NBP2-25183AFP488
Alexa Fluor Plus 555	NBP2-25183AFP555
Alexa Fluor Plus 594	NBP2-25183AFP594
Alexa Fluor Plus 647	NBP2-25183AFP647
Alexa Fluor Plus 680	NBP2-25183AFP680
Allophycocyanin	NBP2-25183APC
Allophycocyanin/Cy7	NBP2-25183APCCY7
Biotin	NBP2-25183B
CoraFluor 1	NBP2-25183CL1
DyLight 350	NBP2-25183UV
DyLight 405	NBP2-25183V
DyLight 488	NBP2-25183G
DyLight 550	NBP2-25183R
DyLight 594	NBP2-25183DL594
DyLight 650	NBP2-25183C
DyLight 680	NBP2-25183FR
DyLight 755	NBP2-25183IR
FITC	NBP2-25183F
HRP	NBP2-25183H
Janelia Fluor 525	NBP2-25183JF525
Janelia Fluor 549	NBP2-25183JF549
Janelia Fluor 646	NBP2-25183JF646
Janelia Fluor 669	NBP2-25183JF669
PE	NBP2-25183PE
PE/Atto594	NBP2-25183PEATT594
PE/Cy5.5	NBP2-25183PECY55
PerCP	NBP2-25183PCP
mFluor Violet 450 SE	NBP2-25183MFV450
mFluor Violet 500 SE	NBP2-25183MFV500
mFluor Violet 610 SE	NBP2-25183MFV610

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NBP2-25154

LAMP-1/CD107a Antibody (5H6) - BSA Free

(6 Publications)

0.1 ml

NBP2-25154

LAMP-1-CD107a-Antibody-5H6-Immunocytochemistry-Immunofluorescence-NBP2-25154-img0008.jpg

D2.0 R cells activate autophagy upon entering a dormant state. Representative images are shown for a Immunofluorescent staining of lysosomal (LAMP1, red) and autophagic (LC3, green) markers of D2.0 R cells in BME (upper panels) and BME + COL1 (lower panels) with b Quantification of the percentage of cells exhibiting LC3 positive puncta and LAMP1 staining out of the total number of cells analysed (mean +/- s.e.m, n = 30–42 cells. Comparisons are relative to Day 1 by Kruskal–Wallis, Dunn’s post test. *P ≤ 0.05; ****P ≤ 0.0001) and the average number of LC3 puncta per cell (Comparisons by Kruskal–Wallis, Dunn’s post test. **P ≤ 0.01 and ****P ≤ 0.0001). c D2.0 R cells transfected with the mCherry-GFP-LC3 reporter show activation and completion of the autophagic cycle (red fluorescence only) when plated in BME matrices for 8 days. d The graphs represent the percentage of cells with double-positive puncta (mCherry+ and GFP+) (autophagosomes) out of the total number of cells analysed (left graph) (mean +/- s.e.m, n = 35–39 cells). Comparisons by Kruskal–Wallis, Dunn’s post test. ****P ≤ 0.0001) and the rate of conversion from cells exhibiting yellow puncta to red-only puncta expressed as fold change (right graph). Scale bars are 50 and 10 μm for a and 20 and 10 μm for b Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29789598), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25154AF350
Alexa Fluor 405	NBP2-25154AF405
Alexa Fluor 488	NBP2-25154AF488
Alexa Fluor 532	NBP2-25154AF532
Alexa Fluor 594	NBP2-25154AF594
Alexa Fluor 647	NBP2-25154AF647
Alexa Fluor 700	NBP2-25154AF700
Alexa Fluor 750	NBP2-25154AF750
Alexa Fluor Plus 405	NBP2-25154AFP405
Alexa Fluor Plus 488	NBP2-25154AFP488
Alexa Fluor Plus 555	NBP2-25154AFP555
Alexa Fluor Plus 594	NBP2-25154AFP594
Alexa Fluor Plus 647	NBP2-25154AFP647
Alexa Fluor Plus 680	NBP2-25154AFP680
Allophycocyanin	NBP2-25154APC
Biotin	NBP2-25154B
CoraFluor 1	NBP2-25154CL1
DyLight 350	NBP2-25154UV
DyLight 405	NBP2-25154V
DyLight 488	NBP2-25154G
DyLight 550	NBP2-25154R
DyLight 594	NBP2-25154DL594
DyLight 650	NBP2-25154C
DyLight 680	NBP2-25154FR
DyLight 755	NBP2-25154IR
FITC	NBP2-25154F
HRP	NBP2-25154H
Janelia Fluor 525	NBP2-25154JF525
Janelia Fluor 549	NBP2-25154JF549
Janelia Fluor 646	NBP2-25154JF646
Janelia Fluor 669	NBP2-25154JF669
PE	NBP2-25154PE
PerCP	NBP2-25154PCP
mFluor Violet 450 SE	NBP2-25154MFV450
mFluor Violet 500 SE	NBP2-25154MFV500
mFluor Violet 610 SE	NBP2-25154MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-25154SS

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AF4800

LAMP-1/CD107a Antibody [Unconjugated]

(1 Review)

(11 Publications)

100 μg

AF4800

HAF016). A specific band was detected for LAMP-1/CD107a at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and HL-60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LAMP-1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4800) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for LAMP-1/CD107a at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and HL-60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LAMP-1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4800) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LAMP-1/CD107a at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

LAMP1/CD107a was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using 10 µg/mL Sheep Anti-Human LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4800) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # <a class=

NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF4800U-100UG
Alexa Fluor 405	AF4800V-100UG
Alexa Fluor 488	IC7985G
Alexa Fluor 532	AF4800X-100UG
Alexa Fluor 594	AF4800T-100UG
Alexa Fluor 647	AF4800R-100UG
Alexa Fluor 700	AF4800N-100UG
Alexa Fluor 750	AF4800S-100UG
Alexa Fluor Plus 405	AF4800AFP405-100UG
Alexa Fluor Plus 488	AF4800AFP488-100UG
Alexa Fluor Plus 555	AF4800AFP555-100UG
Alexa Fluor Plus 594	AF4800AFP594-100UG
Alexa Fluor Plus 647	AF4800AFP647-100UG
Alexa Fluor Plus 680	AF4800AFP680-100UG
Allophycocyanin	AF4800A
Biotin	BAF4800
CoraFluor 1	AF4800CL1
DyLight 350	AF4800D
DyLight 405	AF4800E
DyLight 488	AF4800K
DyLight 550	AF4800L
DyLight 594	AF4800M
DyLight 650	AF4800W
DyLight 680	AF4800Y
DyLight 755	AF4800Z
FITC	AF4800F
HRP	AF4800H
Janelia Fluor 525	AF4800JF525
Janelia Fluor 549	AF4800I
Janelia Fluor 646	AF4800J
Janelia Fluor 669	AF4800JF669
PE	AF4800P
PerCP	AF4800C
mFluor Violet 450 SE	AF4800MFV450
mFluor Violet 500 SE	AF4800MFV500
mFluor Violet 610 SE	AF4800MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC7985G
Carrier Free	AF4800-SP

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NB100-1952

LAMP-1/CD107a Antibody (LY1C6) - BSA Free

(2 Publications)

100 μg

NB100-1952

Reactivity:

Hu, Mu, Rt, Ha

Species Glossary

Reactivity: Hu, Mu, Rt, Ha

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-1952AF350
Alexa Fluor 405	NB100-1952AF405
Alexa Fluor 488	NB100-1952AF488
Alexa Fluor 532	NB100-1952AF532
Alexa Fluor 594	NB100-1952AF594
Alexa Fluor 647	NB100-1952AF647
Alexa Fluor 700	NB100-1952AF700
Alexa Fluor 750	NB100-1952AF750
Alexa Fluor Plus 405	NB100-1952AFP405
Alexa Fluor Plus 488	NB100-1952AFP488
Alexa Fluor Plus 555	NB100-1952AFP555
Alexa Fluor Plus 594	NB100-1952AFP594
Alexa Fluor Plus 647	NB100-1952AFP647
Alexa Fluor Plus 680	NB100-1952AFP680
Allophycocyanin	NB100-1952APC
Biotin	NB100-1952B
CoraFluor 1	NB100-1952CL1
DyLight 350	NB100-1952UV
DyLight 405	NB100-1952V
DyLight 488	NB100-1952G
DyLight 550	NB100-1952R
DyLight 594	NB100-1952DL594
DyLight 650	NB100-1952C
DyLight 680	NB100-1952FR
DyLight 755	NB100-1952IR
FITC	NB100-1952F
HRP	NB100-1952H
Janelia Fluor 525	NB100-1952JF525
Janelia Fluor 549	NB100-1952JF549
Janelia Fluor 646	NB100-1952JF646
Janelia Fluor 669	NB100-1952JF669
PE	NB100-1952PE
PerCP	NB100-1952PCP
mFluor Violet 450 SE	NB100-1952MFV450
mFluor Violet 500 SE	NB100-1952MFV500
mFluor Violet 610 SE	NB100-1952MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-1952-25ug

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MAB4800

LAMP-1/CD107a Antibody (508921) [Unconjugated]

(7 Publications)

100 μg

MAB4800

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to lysosomes in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

LAMP1/CD107a was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human LAMP1/CD107a Monoclonal Antibody (Catalog # MAB4800) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to lysosomes in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="LAMP1/CD107a was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human LAMP1/CD107a Monoclonal Antibody (Catalog # MAB4800) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to lysosomes in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

LAMP-1/CD107a was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human LAMP-1/CD107a Monoclonal Antibody (Catalog # MAB4800) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

$<b>Biological Strategies Validation. </b>Increased SNX9 expression and co-localization with podocin are detectable in the cytoplasm of ADR-treated WT podocytes, whereas SNX9 KD podocytes exhibit little cytoplasmic expression of podocin.(a) Fluorescent micrographs of cultured human podocytes stained with SNX9 (green) and podocin (red) before and after ADR treatment (merged areas are in yellow). DAPI (blue) was used to indicate nuclei. Boxes indicate higher magnification areas presented in the lower panels. (b) Western blot analyses of the fractions from control podocytes or podocytes treated with ADR separated on linear OptiPrep gradients (5–25%). Distributions of SNX9 and podocin, as well as marker proteins of plasma membrane (caveolin), endosome/lysosome (LAMP1), mitochondria ( beta subunit of F1F0-ATPase), and endoplasmic reticulum (calnexin), were examined by western blot analysis. (c) Cultured human podocytes were transfected with nonfunctional control siRNA (upper panel) or SNX9 siRNA (middle and lower panels). Transfected cells, as decided by GFP expression, are indicated by arrowheads. Upper panel: Fluorescent micrographs of control siRNA-transfected podocyte stained with SNX9 (red). Middle panel: Fluorescent micrographs of SNX9 siRNA-transfected podocyte with ADR treatment stained with SNX9 (red). Lower panel: Fluorescent micrographs of SNX9 siRNA-transfected podocyte with ADR treatment stained with podocin (red). DAPI (blue) was used to indicate nuclei. Boxes indicate higher-magnification areas presented on the right. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28266622), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC4800U-100UG
Alexa Fluor 405	IC4800V-100UG
Alexa Fluor 488	IC4800G
Alexa Fluor 532	FAB4800X
Alexa Fluor 594	IC4800T-100UG
Alexa Fluor 647	IC4800R-100UG
Alexa Fluor 700	IC4800N-100UG
Alexa Fluor 750	IC4800S-100UG
Alexa Fluor Plus 405	FAB4800AFP405-100UG
Alexa Fluor Plus 488	FAB4800AFP488-100UG
Alexa Fluor Plus 555	FAB4800AFP555-100UG
Alexa Fluor Plus 594	FAB4800AFP594-100UG
Alexa Fluor Plus 647	FAB4800AFP647-100UG
Alexa Fluor Plus 680	FAB4800AFP680-100UG
Allophycocyanin	IC4800A
Biotin	BAM4800
CoraFluor 1	FAB4800CL1
DyLight 350	FAB4800D
DyLight 405	FAB4800E
DyLight 488	FAB4800K
DyLight 550	FAB4800L
DyLight 594	FAB4800M
DyLight 650	FAB4800W
DyLight 680	FAB4800Y
DyLight 755	FAB4800Z
HRP	FAB4800H
Janelia Fluor 525	FAB4800JF525
Janelia Fluor 549	FAB4800I
Janelia Fluor 646	FAB4800J
Janelia Fluor 669	FAB4800JF669
PerCP	FAB4800C
Phycoerythrin	IC4800P
mFluor Violet 450 SE	FAB4800MFV450
mFluor Violet 500 SE	FAB4800MFV500
mFluor Violet 610 SE	FAB4800MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC4800A
Carrier Free	IC4800G
Carrier Free	IC4800P
Carrier Free	MAB4800-SP
Carrier Free	IC4800S-100UG
Carrier Free	IC4800R-100UG
Carrier Free	IC4800N-100UG
Carrier Free	IC4800V-100UG
Carrier Free	IC4800U-100UG
Carrier Free	IC4800T-100UG

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NBP1-77241

LAMP-1/CD107a Antibody - BSA Free

(2 Publications)

0.1 mg

NBP1-77241

Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NBP1-77241] - Immunofluorescence of LAMP-1/CD107a in Human Colon cells with LAMP-1/CD107a antibody at 20 ug/mL.

Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NBP1-77241] - Immunofluorescence of LAMP-1/CD107a in rat colon tissue with LAMP-1/CD107a antibody at 20 ug/ml.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77241AF350
Alexa Fluor 405	NBP1-77241AF405
Alexa Fluor 488	NBP1-77241AF488
Alexa Fluor 532	NBP1-77241AF532
Alexa Fluor 594	NBP1-77241AF594
Alexa Fluor 647	NBP1-77241AF647
Alexa Fluor 700	NBP1-77241AF700
Alexa Fluor 750	NBP1-77241AF750
Alexa Fluor Plus 405	NBP1-77241AFP405
Alexa Fluor Plus 488	NBP1-77241AFP488
Alexa Fluor Plus 555	NBP1-77241AFP555
Alexa Fluor Plus 594	NBP1-77241AFP594
Alexa Fluor Plus 647	NBP1-77241AFP647
Alexa Fluor Plus 680	NBP1-77241AFP680
Allophycocyanin	NBP1-77241APC
Biotin	NBP1-77241B
CoraFluor 1	NBP1-77241CL1
DyLight 350	NBP1-77241UV
DyLight 405	NBP1-77241V
DyLight 488	NBP1-77241G
DyLight 550	NBP1-77241R
DyLight 594	NBP1-77241DL594
DyLight 650	NBP1-77241C
DyLight 680	NBP1-77241FR
DyLight 755	NBP1-77241IR
FITC	NBP1-77241F
HRP	NBP1-77241H
Janelia Fluor 525	NBP1-77241JF525
Janelia Fluor 549	NBP1-77241JF549
Janelia Fluor 646	NBP1-77241JF646
Janelia Fluor 669	NBP1-77241JF669
PE	NBP1-77241PE
PerCP	NBP1-77241PCP
mFluor Violet 450 SE	NBP1-77241MFV450
mFluor Violet 500 SE	NBP1-77241MFV500
mFluor Violet 610 SE	NBP1-77241MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77241-0.025mg

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NBP2-59780

LAMP-1/CD107a Antibody (CL3484) - BSA Free

(1 Review)

100 ul

NBP2-59780

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human liver shows strong granular cytoplasmic immunoreactivity in hepatocytes.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human fallopian tube shows granular cytoplasmic positivity in epithelial cells.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human endometrium shows granular cytoplasmic immunoreactivity in epithelial and stromal cells.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (3916) [NBP2-59780] - Staining of human prostate shows strong granular cytoplasmic positivity in epithelial cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-43999AF350
Alexa Fluor 405	NBP3-43999AF405
Alexa Fluor 488	NBP3-43999AF488
Alexa Fluor 532	NBP3-43999AF532
Alexa Fluor 594	NBP3-43999AF594
Alexa Fluor 647	NBP3-43999AF647
Alexa Fluor 700	NBP3-43999AF700
Alexa Fluor 750	NBP3-43999AF750
Alexa Fluor Plus 405	NBP3-43999AFP405
Alexa Fluor Plus 488	NBP3-43999AFP488
Alexa Fluor Plus 555	NBP3-43999AFP555
Alexa Fluor Plus 594	NBP3-43999AFP594
Alexa Fluor Plus 647	NBP3-43999AFP647
Alexa Fluor Plus 680	NBP3-43999AFP680
Allophycocyanin	NBP3-43999APC
Biotin	NBP3-43999B
CoraFluor 1	NBP3-43999CL1
DyLight 350	NBP3-43999UV
DyLight 405	NBP3-43999V
DyLight 488	NBP3-43999G
DyLight 550	NBP3-43999R
DyLight 594	NBP3-43999DL594
DyLight 650	NBP3-43999C
DyLight 680	NBP3-43999FR
DyLight 755	NBP3-43999IR
FITC	NBP3-43999F
HRP	NBP3-43999H
Janelia Fluor 525	NBP3-43999JF525
Janelia Fluor 549	NBP3-43999JF549
Janelia Fluor 646	NBP3-43999JF646
Janelia Fluor 669	NBP3-43999JF669
PE	NBP3-43999PE
PerCP	NBP3-43999PCP
mFluor Violet 450 SE	NBP3-43999MFV450
mFluor Violet 500 SE	NBP3-43999MFV500
mFluor Violet 610 SE	NBP3-43999MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-43999
BSA Free	NBP2-59780-25ul

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NB600-956

LAMP-1/CD107a Antibody - BSA Free

(1 Review)

(4 Publications)

0.1 ml

NB600-956

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-956AF350
Alexa Fluor 405	NB600-956AF405
Alexa Fluor 488	NB600-956AF488
Alexa Fluor 532	NB600-956AF532
Alexa Fluor 594	NB600-956AF594
Alexa Fluor 647	NB600-956AF647
Alexa Fluor 700	NB600-956AF700
Alexa Fluor 750	NB600-956AF750
Alexa Fluor Plus 405	NB600-956AFP405
Alexa Fluor Plus 488	NB600-956AFP488
Alexa Fluor Plus 555	NB600-956AFP555
Alexa Fluor Plus 594	NB600-956AFP594
Alexa Fluor Plus 647	NB600-956AFP647
Alexa Fluor Plus 680	NB600-956AFP680
Allophycocyanin	NB600-956APC
Biotin	NB600-956B
CoraFluor 1	NB600-956CL1
DyLight 350	NB600-956UV
DyLight 405	NB600-956V
DyLight 488	NB600-956G
DyLight 550	NB600-956R
DyLight 594	NB600-956DL594
DyLight 650	NB600-956C
DyLight 680	NB600-956FR
DyLight 755	NB600-956IR
FITC	NB600-956F
HRP	NB600-956H
Janelia Fluor 525	NB600-956JF525
Janelia Fluor 549	NB600-956JF549
Janelia Fluor 646	NB600-956JF646
Janelia Fluor 669	NB600-956JF669
PE	NB600-956PE
PerCP	NB600-956PCP
mFluor Violet 450 SE	NB600-956MFV450
mFluor Violet 500 SE	NB600-956MFV500
mFluor Violet 610 SE	NB600-956MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-956-0.025ml

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NBP2-61619

LAMP-1/CD107a Antibody (CL3482) - BSA Free

(1 Publication)

100 ul

NBP2-61619

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human kidney shows strong granular cytoplasmic immunoreactivity in renal glomerulus and tubules cells.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human fallopian tube shows granular cytoplasmic immunoreactivity in epithelial cells.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human small intestine shows granular cytoplasmic immunoreactivity in glandular cells.

$LAL protein accumulates in the extra-lysosomal compartment in an in vitro model of NAFLD.Huh7 cells were cultured in high-glucose/high-lipid (HGHL) medium for 8 days. A–C Analysis of LAL localization by confocal microscopy. The lysosomal marker LAMP1 (red) and total LAL protein (individuated by antibody against C-term) (green) were shown (A) and their abundance (B) and colocalization (C) were evaluated. Scale bar 25 μm. D, E Western blot analysis of LAL C-terminal domain, LDHB, Tubulin and LAMP-1 in in the cytosolic fraction (D) and evaluation of LAL content in the cytosolic fraction by calculating LAL to tubulin ratio through densitometric analysis (E). Representative images from one experiment out of three giving similar results are reported. Data are expressed as mean +/- SD (*p < 0.05, **p < 0.01 ***p < 0.001, vs CTR; n = 3). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34795230), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-43861AF350
Alexa Fluor 405	NBP3-43861AF405
Alexa Fluor 488	NBP3-43861AF488
Alexa Fluor 532	NBP3-43861AF532
Alexa Fluor 594	NBP3-43861AF594
Alexa Fluor 647	NBP3-43861AF647
Alexa Fluor 700	NBP3-43861AF700
Alexa Fluor 750	NBP3-43861AF750
Alexa Fluor Plus 405	NBP3-43861AFP405
Alexa Fluor Plus 488	NBP3-43861AFP488
Alexa Fluor Plus 555	NBP3-43861AFP555
Alexa Fluor Plus 594	NBP3-43861AFP594
Alexa Fluor Plus 647	NBP3-43861AFP647
Alexa Fluor Plus 680	NBP3-43861AFP680
Allophycocyanin	NBP3-43861APC
Biotin	NBP3-43861B
CoraFluor 1	NBP3-43861CL1
DyLight 350	NBP3-43861UV
DyLight 405	NBP3-43861V
DyLight 488	NBP3-43861G
DyLight 550	NBP3-43861R
DyLight 594	NBP3-43861DL594
DyLight 650	NBP3-43861C
DyLight 680	NBP3-43861FR
DyLight 755	NBP3-43861IR
FITC	NBP3-43861F
HRP	NBP3-43861H
Janelia Fluor 525	NBP3-43861JF525
Janelia Fluor 549	NBP3-43861JF549
Janelia Fluor 646	NBP3-43861JF646
Janelia Fluor 669	NBP3-43861JF669
PE	NBP3-43861PE
PerCP	NBP3-43861PCP
mFluor Violet 450 SE	NBP3-43861MFV450
mFluor Violet 500 SE	NBP3-43861MFV500
mFluor Violet 610 SE	NBP3-43861MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-43861
BSA Free	NBP2-61619-25ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

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Species Glossary

LAMP1 Products By Gene Name

LAMP-1/CD107a Antibody - BSA Free

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LAMP-1/CD107a Antibody [Unconjugated]

LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

LAMP-1/CD107a Antibody (5H6) - BSA Free

LAMP-1/CD107a Antibody [Unconjugated]

LAMP-1/CD107a Antibody (LY1C6) - BSA Free

LAMP-1/CD107a Antibody (508921) [Unconjugated]

LAMP-1/CD107a Antibody - BSA Free

LAMP-1/CD107a Antibody (CL3484) - BSA Free

LAMP-1/CD107a Antibody - BSA Free

LAMP-1/CD107a Antibody (CL3482) - BSA Free