Lactate Dehydrogenase B Antibody (2057D) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Lactate Dehydrogenase B/LDHB at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western shows lysates of Exosome Standard (LNCaP) (NBP3-11687) and PC‑3 human prostate cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for Lactate Dehydrogenase B/LDHB at approximately 38 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Lactate Dehydrogenase B/LDHB was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Lactate Dehydrogenase B/LDHB was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

HeLa human cervical epithelial carcinoma cell line was stained with Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and LDHB knockout HEK293T cell line (KO). PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Lactate Dehydrogenase B/LDHB at approximately 36 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, Flow, ICC/IF, KO
• Clone: 2057D
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Lactate Dehydrogenase B Antibody (2057D) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant human LDHB
  Met1-Val93
  Accession # P07195
• Specificity: Detects human LDHB in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 1-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Knockout Validated
  • Simple Western 10 ug/mL
  • Western Blot 0.05 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitue at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lactate Dehydrogenase B Antibody (2057D) [Unconjugated]

• EC 1.1.1
• EC 1.1.1.27
• Epididymis Secretory Protein Li 281
• HEL-S-281
• Lactate Dehydrogenase B
• LDH heart subunit
• LDHB
• LDH-B
• LDHBD
• LDH-H
• L-lactate dehydrogenase B chain
• Renal Carcinoma Antigen NY-REN-46
• TRG-5

Background

LDHB is one of two subunits of Lactose Dehydrogenase which is formed from tetramer arrangements of the A and B subunits.  LDHB is part of the pyruvate fermentation to lactate pathway in which it catalyzes the conversion of L-lactate and NAD to pyruvate and NADH.  LDHA is widely expressed, with high expression in muscle and brain.  High expression in tumor cells correlates with low survival rates in various cancers including osteosarcoma, oral squamous cell carcinoma breast and lung cancers. Unlike Lactose Dehyrogenase A, LDHB mutations are not linked to any disease

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lactate Dehydrogenase B Antibody (MAB9205)(6)

Publications using MAB9205

• Deng, H;Malsiu, F;Ge, H;Losmanova, T;Medová, M;Zamboni, N;Wang, W;Peng, RW;Tang, J;Dorn, P;Marti, TM; LDHB silencing enhances the effects of radiotherapy by impairing nucleotide metabolism and promoting persistent DNA damage Scientific reports 2025-03-29 [PMID: 40158058] (Western Blot, Immunocytochemistry, Human)
• Deng, H;Zhao, L;Ge, H;Gao, Y;Fu, Y;Lin, Y;Masoodi, M;Losmanova, T;Medová, M;Ott, J;Su, M;Wang, W;Peng, RW;Dorn, P;Marti, TM; Ubiquinol-mediated suppression of mitochondria-associated ferroptosis is a targetable function of lactate dehydrogenase B in cancer Nature communications 2025-03-16 [PMID: 40090955] (Immunohistochemistry, Western Blot, Human, Xenograft)
• Frank, AR;Vandiver, F;McFadden, DG; Forward genetic screens identify mechanisms of resistance to small molecule lactate dehydrogenase inhibitors bioRxiv : the preprint server for biology 2023-09-30 [PMID: 37808702] (Western Blot, Human)
• San-Millan, I;Martinez, JL;Pickard, SL;Yu, H;Hirsch, FR;Rivard, CJ;Brooks, GA; Role of Lactate in the Regulation of Transcriptional Activity of Breast Cancer-Related Genes and Epithelial-to-Mesenchymal Transition Proteins: A Comparison of MCF7 and MDA-MB-231 Cancer Cell Lines bioRxiv : the preprint server for biology 2023-09-07 [PMID: 36993762] (Western Blot, Human)
• M La Montagn, L Shi, P Magee, S Sahoo, M Fassan, M Garofalo AMPK&alpha loss promotes KRAS-mediated lung tumorigenesis Cell Death and Differentiation, 2021-05-26;0(0):. 2021-05-26 [PMID: 34040167] (Western Blot, Human)
• J Albanesi, NI Noguera, C Banella, T Colangelo, E De Marinis, S Leone, O Palumbo, MT Voso, P Ascenzi, C Nervi, F Bianchi, A di Masi Transcriptional and Metabolic Dissection of ATRA-Induced Granulocytic Differentiation in NB4 Acute Promyelocytic Leukemia Cells Cells, 2020-11-05;9(11):. 2020-11-05 [PMID: 33167477] (Western Blot, Human)

Bioinformatics

• Uniprot:
  • P07195()