Lactate Dehydrogenase Antibody [Alexa Fluor™ Plus 647]

• Reactivity: Hu, Rb
• Applications: WB, ELISA, ICC/IF, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor Plus 647

Lactate Dehydrogenase Antibody [Alexa Fluor™ Plus 647] Summary

• Immunogen: This Lactate Dehydrogenase Antibody was prepared from whole goat serum produced by repeated immunizations with a full length Lactate Dehydrogenase protein isolated from rabbit muscle. (Uniprot: P13491)
• Localization: Cytoplasmic
• Specificity: BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LDHB
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Multi-step

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Lactate Dehydrogenase Antibody [Alexa Fluor™ Plus 647]

• EC 1.1.1
• EC 1.1.1.27
• lactate dehydrogenase B
• LDH heart subunit
• LDH-B
• LDH-H
• L-lactate dehydrogenase B chain
• Renal carcinoma antigen NY-REN-46
• TRG-5

Background

Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDHA, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H or M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) -in the heart, LDH-2 (3H1M) -in the reticuloendothelial system, LDH-3 (2H2M) -in the lungs, LDH-4 (1H3M) -in the kidneys and LDH-5 (4M) -in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a "flipped pattern") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LDHB