Lactate Dehydrogenase Antibody

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Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ...read more
Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase Antibody [NB600-861] - Analysis of Biotin conjugate of NB600-861. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked with incubated ...read more
Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using Biotin conjugate of NB600-861. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate ...read more
Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using Biotin conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: ...read more
Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate ...read more
Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase Antibody [NB600-861] - Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked with incubated with blocking buffer for 15 min. Antigen ...read more
Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase Antibody [NB600-861] - Analysis of HRP conjugate of NB600-861. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked with MB-071 ...read more

Product Details

Summary
Reactivity Hu, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

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Catalog# & Conjugate Size Price

Lactate Dehydrogenase Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Lactate Dehydrogenase [Rabbit Muscle].
Localization
Cytoplasmic
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LDHB
Purity
Ion exchange chromatography
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • ELISA 1:2000 - 1:10000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:2000
  • Immunoprecipitation 1:100
Application Notes
This antibody has been tested for use in ELISA and by western blot. Expect a band approximately 36 kDa in size corresponding - LDH western blotting in the appropriate cell lysate or extract.
Publications
Read Publications using NB600-861.

Reactivity Notes

Cross-reacts with Rabbit. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for Lactate Dehydrogenase Antibody

  • EC 1.1.1
  • EC 1.1.1.27
  • lactate dehydrogenase B
  • LDH heart subunit
  • LDH-B
  • LDH-H
  • L-lactate dehydrogenase B chain
  • Renal carcinoma antigen NY-REN-46
  • TRG-5

Background

Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDHA, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H or M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) -in the heart, LDH-2 (3H1M) -in the reticuloendothelial system, LDH-3 (2H2M) -in the lungs, LDH-4 (1H3M) -in the kidneys and LDH-5 (4M) -in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a "flipped pattern") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lactate Dehydrogenase Antibody (NB600-861)(3)


Showing Publications 1 - 3 of 3.
Publications using NB600-861 Applications Species
Koukourakis MI et al. Lactate dehydrogenase-5 (LDH-5) overexpression in non-small-cell lung cancer tissues is linked to tumour hypoxia, angiogenic factor production poor prognosis Br J Cancer 2003 [PMID: 12942121]

Details:
This citation used the HRP version of this antibody.
Crawford RM et al. M-LDH serves as a sarcolemmal K(ATP) channel subunit essential for cell protection against ischemia. EMBO J 21:3936-48. 2002 [PMID: 12145195]
Koukourakis MI et al. Lactate dehydrogenase-5 (LDH-5) overexpression in non-small-cell lung cancer tissues is linked to tumour hypoxia, angiogenic factor production poor prognosis Br J Cancer Jun 25 1905

Details:
This publication used the HRP conjugated form of this antibody (Cat# NB600-863).

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Bioinformatics

Gene Symbol LDHB
Entrez
Uniprot