Reactivity | Hu, RbSpecies Glossary |
Applications | WB, ELISA, ICC/IF, IP |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Unconjugated |
Concentration | LYOPH |
Conjugate | Catalog # | Availability | Size | Price |
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Biotin | NB600-862-0.1mg | |||
HRP | NB600-863-0.1mg | |||
Description | This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. |
Immunogen | This Lactate Dehydrogenase Antibody was prepared from whole goat serum produced by repeated immunizations with a full length Lactate Dehydrogenase protein isolated from rabbit muscle. (Uniprot: P13491) |
Localization | Cytoplasmic |
Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | LDHB |
Purity | Multi-step |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 36 kDa in size corresponding to LDH western blotting in the appropriate cell lysate or extract. |
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Theoretical MW | 36.56 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Publications |
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Storage | Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Preservative | 0.01% Sodium Azide |
Concentration | LYOPH |
Purity | Multi-step |
Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent) |
Publications using NB600-861 | Applications | Species |
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Koukourakis MI et al. Lactate dehydrogenase-5 (LDH-5) overexpression in non-small-cell lung cancer tissues is linked to tumour hypoxia, angiogenic factor production poor prognosis Br J Cancer 2003 [PMID: 12942121] Details: This citation used the HRP version of this antibody. |
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Koukourakis MI et al. Lactate dehydrogenase-5 (LDH-5) overexpression in non-small-cell lung cancer tissues is linked to tumour hypoxia, angiogenic factor production poor prognosis Br J Cancer Jun 25 1905 Details: This publication used the HRP conjugated form of this antibody (Cat# NB600-863). |
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Crawford RM et al. M-LDH serves as a sarcolemmal K(ATP) channel subunit essential for cell protection against ischemia. EMBO J 21:3936-48. 2002 [PMID: 12145195] |
Secondary Antibodies |
Isotype Controls |
Diseases for Lactate Dehydrogenase Antibody (NB600-861)Discover more about diseases related to Lactate Dehydrogenase Antibody (NB600-861).
| Pathways for Lactate Dehydrogenase Antibody (NB600-861)View related products by pathway.
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