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Recombinant E. coli Lactate Dehydrogenase A/LDHA His Protein

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SDS-Page: Recombinant E. coli Lactate Dehydrogenase A/LDHA Protein [NBP2-52236] - 15% SDS Page (3 ug)

Product Details

Summary
Reactivity EcSpecies Glossary
Applications PAGE, Enzyme Activity
Concentration
1 mg/ml

Order Details

Recombinant E. coli Lactate Dehydrogenase A/LDHA His Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-329 of E.coli Lactate Dehydrogenase A/LDHA

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMKLAVY STKQYDKKYL QQVNESFGFE LEFFDFLLTE KTAKTANGCE AVCIFVNDDG SRPVLEELKK HGVKYIALRC AGFNNVDLDA AKELGLKVVR VPAYDPEAVA EHAIGMMMTL NRRIHRAYQR TRDANFSLEG LTGFTMYGKT AGVIGTGKIG VAMLRILKGF GMRLLAFDPY PSAAALELGV EYVDLPTLFS ESDVISLHCP LTPENYHLLN EAAFEQMKNG VMIVNTSRGA LIDSQAAIEA LKNQKIGSLG MDVYENERDL FFEDKSNDVI QDDVFRRLSA CHNVLFTGHQ AFLTAEALTS ISQTTLQNLS NLEKGETCPN ELV

Details of Functionality
Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
LDHA
Purity
>95%, by SDS-PAGE
Endotoxin Note
< 1 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

Dilutions
  • Enzyme Activity
  • SDS-Page
Theoretical MW
39.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 100 mM NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant E. coli Lactate Dehydrogenase A/LDHA His Protein

  • Cell proliferation-inducing gene 19 protein
  • EC 1.1.1
  • EC 1.1.1.27
  • GSD11
  • Lactate Dehydrogenase A
  • lactate dehydrogenase M
  • LDH muscle subunit
  • LDH1
  • LDHA
  • LDH-A
  • LDHM
  • LDH-M
  • L-lactate dehydrogenase A chain
  • proliferation-inducing gene 19
  • Renal carcinoma antigen NY-REN-59

Background

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C, forming various terameric isoenzymes (140 kDa). Lactate dehydrogenase A (LDH-A, muscle subunit, LDH-M) is involved in the final step of anaerobic glycoysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that cause deficiency in LDH-A has been implicated in exertional myoglobinuria (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol LDHA