Lactate Dehydrogenase A/LDHA Antibody (1A4)

Images

 
Western Blot: Lactate Dehydrogenase A/LDHA Antibody (1A4) [NBP1-74023] - Lane 1.: Jurkat cell lysate Lane 2.: 293T cell lysate Lane 3.: A431 cell lysate. antibody (1:1000).
Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (1A4) [NBP1-74023] - HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human LDHA antibody (1:500) with ...read more
Flow Cytometry: Lactate Dehydrogenase A/LDHA Antibody (1A4) [NBP1-74023] - Flow cytometry analysis of LDHA in HeLa cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat antimouse IgG Alexa fluor ...read more
Western Blot: Lactate Dehydrogenase A/LDHA Antibody (1A4) [NBP1-74023] - Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human LDHA (1:8000). Proteins were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
1A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Lactate Dehydrogenase A/LDHA Antibody (1A4) Summary

Immunogen
Recombinant human LDHA (1-332aa) purified from E.coli (NP_005557)
Specificity
Anti-human LDHA mAb, clone 1A4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human LDHA protein.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LDHA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:8000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:500 ~ 8,000. Recommended starting dilution is 1:1,000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Lactate Dehydrogenase A/LDHA Antibody (1A4)

  • Cell proliferation-inducing gene 19 protein
  • EC 1.1.1
  • EC 1.1.1.27
  • GSD11
  • Lactate Dehydrogenase A
  • lactate dehydrogenase M
  • LDH muscle subunit
  • LDH1
  • LDHA
  • LDH-A
  • LDHM
  • LDH-M
  • L-lactate dehydrogenase A chain
  • proliferation-inducing gene 19
  • Renal carcinoma antigen NY-REN-59

Background

Lactate through lactate dehydrogenase A (LDHA), which is encoded by a target gene of c-Myc and hypoxia-inducible factor (HIF-1). LDHA expression is repressed by SugR in the absence of sugar. Reduction of LDHA causes bioenergetic and oxidative stress leading to cell death. LDHA (LDH-5, M-LDH, or A4), which is the predominant form in skeletal muscle, kinetically favors the conversion of pyruvate to lactate. Therefore, LDH-A is an attractive target for cancer therapy since its expression is largely relegated to skeletal muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-74023AF405
Alexa Fluor 488 Labeled NBP1-74023AF488
Alexa Fluor 647 Labeled NBP1-74023AF647
Alexa Fluor 700 Labeled NBP1-74023AF700
DyLight 350 Labeled NBP1-74023UV
DyLight 405 Labeled NBP1-74023V
DyLight 488 Labeled NBP1-74023G
DyLight 680 Labeled NBP1-74023FR
DyLight 755 Labeled NBP1-74023IR

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Bioinformatics

Gene Symbol LDHA
Entrez
Uniprot