Lactate Dehydrogenase A/LDHA Antibody (1A4) - BSA Free

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (1A4) [NBP1-74023] - Lane 1.: Jurkat cell lysate Lane 2.: 293T cell lysate Lane 3.: A431 cell lysate. antibody (1:1000).

Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (1A4) [NBP1-74023] - HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human LDHA antibody (1:500) with Texas Red (Red).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Lactate Dehydrogenase A/LDHA Antibody (1A4) - BSA Free Summary

• Immunogen: Recombinant human Lactate Dehydrogenase A/LDHA (1-332aa) purified from E.coli (NP_005557)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LDHA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Western Blot 1:1000-1:8000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Lactate Dehydrogenase A/LDHA Antibody (1A4) - BSA Free

• Cell proliferation-inducing gene 19 protein
• EC 1.1.1
• EC 1.1.1.27
• GSD11
• Lactate Dehydrogenase A
• lactate dehydrogenase M
• LDH muscle subunit
• LDH1
• LDHA
• LDH-A
• LDHM
• LDH-M
• L-lactate dehydrogenase A chain
• proliferation-inducing gene 19
• Renal carcinoma antigen NY-REN-59

Background

Lactate through lactate dehydrogenase A (LDHA), which is encoded by a target gene of c-Myc and hypoxia-inducible factor (HIF-1). LDHA expression is repressed by SugR in the absence of sugar. Reduction of LDHA causes bioenergetic and oxidative stress leading to cell death. LDHA (LDH-5, M-LDH, or A4), which is the predominant form in skeletal muscle, kinetically favors the conversion of pyruvate to lactate. Therefore, LDH-A is an attractive target for cancer therapy since its expression is largely relegated to skeletal muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lactate Dehydrogenase A/LDHA Antibody (NBP1-74023)(2)

Publications using NBP1-74023

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01
• Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)
  
  Details:
  Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).

Bioinformatics

• Gene Symbol: LDHA
• Entrez:
  • 3939(Human)
• Uniprot:
  • P00338(Human)