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KLF4 Antibody

Images

 
Immunohistochemistry: KLF4 Antibody [NBP2-24749] - DAB IHC staining. Representative 3,3-diaminobenzidine immunohistochemical-stained images showing protein expression of induced-pluripotent stem cell marker KLF4 (J-L, ...read more
Western Blot: KLF4 Antibody [NBP2-24749] - Total protein from Human PC3, Hek293 and Mouse Neuro2A cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Immunohistochemistry: KLF4 Antibody [NBP2-24749] - IF IHC staining. Representative immunofluorescence immunohistochemical-stained images showing protein expression of induced-pluripotent stem cell markers OCT4 (A-C, ...read more
Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Representative immunofluorescence immunohistochemical-stained sections of colon adenocarcinoma metastasis to the liver showing the expression of KLF4 [red] on ...read more
Flow Cytometry: KLF4 Antibody [NBP2-24749] - Intracellular analysis using KLF4 antibody. Balb/c mouse splenocytes were probed using 1 ug of KLF4 antibody (red) and 1 ug of isotype control was used for this test, and an ...read more
Western Blot: KLF4 Antibody [NBP2-24749] - Lysate from human 293 cells in the (1) absence and (2) presence of immunizing peptide, (3) mouse NIH 3T3, and (4) RAW cells probed with KLF4 antibody at 1 ug/ml (293) and 2 ...read more
Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Breast cancer tissues embedded in paraffin. Negative control is no primary antibody. IHC-P image submitted by a verified customer review.
Representative Western blot images of total protein extracted from four head and neck metastatic malignant melanoma-derived primary cell lines probed for induced pluripotent stem cell markers. Blots were probed for OCT4 ...read more
C/EBPδ promotes HIF-1 alpha expression in macrophages through inhibition of FBXW7 alpha a) RT-PCR analysis of FBXW7 isoform expression from different sources as follows. 1, primary peritoneal macrophages (PPMs); 2, ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, Bv, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

KLF4 Antibody Summary

Immunogen
A portion of amino acids 1-50 of human KLF4 was used as the immunogen for this antibody.
Predicted Species
Bovine (97%), Chimpanzee (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KLF4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1 ug/10^6 cells
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500. Use reported in scientific literature (PMID 34428252)
  • Immunohistochemistry reported in scientific literature (PMID 34428252; 30901290)
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-3 ug/ml
Reviewed Applications
Read 2 Reviews rated 2.5
using
NBP2-24749 in the following applications:

Publications
Read Publications using
NBP2-24749 in the following applications:

Reactivity Notes

The amino acid sequence used as immunogen for the antibody is 100% homologous in human and chimpanzee. Mouse reactivity reported in scientific publication.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for KLF4 Antibody

  • Epithelial zinc finger protein EZF
  • EZF
  • EZFendothelial Kruppel-like zinc finger protein
  • GKLFKrueppel-like factor 4
  • Gut-enriched krueppel-like factor
  • KLF4
  • Kruppel-like factor 4 (gut)

Background

KLF4 is a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene. It binds the CACCC core sequence. KLF4 may be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 also has roles as both an oncogene and a tumor suppressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KLF4 Antibody (NBP2-24749)(26)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: B/N, ICC/IF, IF/IHC, IHC, IHC-P, ISH, WB.


Filter By Application
B/N
(1)
ICC/IF
(3)
IF/IHC
(5)
IHC
(2)
IHC-P
(7)
ISH
(1)
WB
(12)
All Applications
Filter By Species
Human
(13)
Mouse
(3)
Rat
(2)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NBP2-24749 Applications Species
Shlepova OV, Shulepko MA, Shipunova VO et al. Selective targeting of ?7 nicotinic acetylcholine receptor by synthetic peptide mimicking loop I of human SLURP-1 provides efficient and prolonged therapy of epidermoid carcinoma in vivo Frontiers in cell and developmental biology 2023-10-03 [PMID: 37854069] (WB, Mouse)

Details:
1:2000 dilution
WB Mouse
Imai T, Van TM, Pasparakis M, Polykratis A Smooth muscle cell specific NEMO deficiency inhibits atherosclerosis in ApoE-/- mice Scientific reports 2022-07-22 [PMID: 35869246] (WB, Mouse) WB Mouse
Paterson C, Kilmister EJ, Brasch HD Et al. Cell Populations Expressing Stemness-Associated Markers in Lung Adenocarcinoma Life (Basel, Switzerland) 2021-10-18 [PMID: 34685477] (IHC-P, Human) IHC-P Human
Munro MJ, Peng L, Wickremesekera SK, Tan ST Colon adenocarcinoma-derived cells possessing stem cell function can be modulated using renin-angiotensin system inhibitors PloS one 2021-08-24 [PMID: 34428252] (ICC/IF, IF/IHC) ICC/IF, IF/IHC
Luke Krishnan CS, Brasch HD, Patel J et al. Stemness-Associated Markers Are Expressed in Extracranial Arteriovenous Malformation Frontiers in Surgery 2021-03-19 [PMID: 33816543]
Williams J, Brasch H, Bockett N et al. Embryonic Stem Cell-like Population in Hypertrophic Port-wine Stain Journal of Vascular Anomalies 2021-02-11 (WB, IHC-P, Human) WB, IHC-P Human
Yamamoto H, Uchida Y, Chiba T et al. Transcriptome analysis of sevoflurane exposure effects at the different brain regions PLOS ONE 2020-12-15 [PMID: 33320849] (IHC, B/N, WB) IHC, B/N, WB
Kilmister E, Patel J, Bockett N et al. Embryonic stem cell-like subpopulations are present within Schwannoma Journal of Clinical Neuroscience 2020-11-01 [PMID: 33222917] (IF/IHC, Human) IF/IHC Human
Kilmister EJ, Patel J, van Schaijik B et al. Cancer Stem Cell Subpopulations Are Present Within Metastatic Head and Neck Cutaneous Squamous Cell Carcinoma Frontiers in Oncology 2020-07-30 [PMID: 32850316] (IHC, ICC/IF) IHC, ICC/IF
Munro MJ, Peng L, Wickremesekera SK, Tan ST Colon adenocarcinoma-derived cells that express induced-pluripotent stem cell markers possess stem cell function PLoS ONE 2020-05-19 [PMID: 32428045] (WB, Human) WB Human
Show All 26 Publications.

Reviews for KLF4 Antibody (NBP2-24749) (2) 2.52

Average Rating: 2.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Bovine.

Reviews using NBP2-24749:
Filter by Applications
IHC-Fr
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Bovine
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen KLF4 NBP2-24749
Enlarge
1
reviewed by:
Alena Zelinka
IHC-Fr Bovine 12/03/2021
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedskin
SpeciesBovine
Lot05232589B-08

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***
Immunohistochemistry-Paraffin KLF4 NBP2-24749
Enlarge
4
reviewed by:
Liyong Zhang
IHC-P Human 08/21/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for KLF4 Antibody (NBP2-24749) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional KLF4 Products

Research Areas for KLF4 Antibody (NBP2-24749)

Find related products by research area.

Blogs on KLF4.

Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness
By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax...  Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

KLF4 opens the door for stem cell research
KLF4 (Kruppel-like factor 4, Epithelial zinc finger protein EZF) is a zinc finger transcription factor thought to be involved in developmental differentiation and proliferation. It is considered a pluripotency reprogramming factor (PRF) due to its abi...  Read full blog post.

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Recent Reviews

2.5
5
0
4
1
3
0
2
0
1
1

Alena Zelinka
12/03/2021
Application: IHC-Fr
Species: Bovine

Liyong Zhang
08/21/2014
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol KLF4
Uniprot