KLF4 Antibody

Images

 
Immunohistochemistry: KLF4 Antibody [NBP2-24749] - DAB IHC staining. Representative 3,3-diaminobenzidine immunohistochemical-stained images showing protein expression of induced-pluripotent stem cell marker KLF4 (J-L, ...read more
Western Blot: KLF4 Antibody [NBP2-24749] - Total protein from Human PC3, Hek293 and Mouse Neuro2A cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Immunohistochemistry: KLF4 Antibody [NBP2-24749] - IF IHC staining. Representative immunofluorescence immunohistochemical-stained images showing protein expression of induced-pluripotent stem cell markers OCT4 (A-C, ...read more
Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Representative immunofluorescence immunohistochemical-stained sections of colon adenocarcinoma metastasis to the liver showing the expression of KLF4 [red] on ...read more
Flow Cytometry: KLF4 Antibody [NBP2-24749] - Intracellular analysis using KLF4 antibody. Balb/c mouse splenocytes were probed using 1 ug of KLF4 antibody (red) and 1 ug of isotype control was used for this test, and an ...read more
Western Blot: KLF4 Antibody [NBP2-24749] - Lysate from human 293 cells in the (1) absence and (2) presence of immunizing peptide, (3) mouse NIH 3T3, and (4) RAW cells probed with KLF4 antibody at 1 ug/ml (293) and 2 ...read more
Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Breast cancer tissues embedded in paraffin. Negative control is no primary antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

KLF4 Antibody Summary

Immunogen
A portion of amino acids 1-50 of human KLF4 was used as the immunogen for this antibody.
Specificity
This anitbody will detect a number of isoforms of the FUZ protein ranging from 269 amino acids to 418 amino acids. Multiple bands may be seen in Western blotting.
Predicted Species
Chimpanzee (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KLF4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Flow Cytometry 1 ug/10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID: 30901291).
Control
KLF4 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NBP2-24749 in the following applications:

Publications
Read Publications using
NBP2-24749 in the following applications:

  • 1 publication
  • IHC
    4 publications
  • 6 publications
  • ISH
    1 publication
  • WB
    8 publications

Reactivity Notes

The amino acid sequence used as immunogen for the antibody is 100% homologous in human and chimpanzee. Mouse reactivity reported in scientific publication.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.1 ml PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for KLF4 Antibody

  • Epithelial zinc finger protein EZF
  • EZF
  • EZFendothelial Kruppel-like zinc finger protein
  • GKLFKrueppel-like factor 4
  • Gut-enriched krueppel-like factor
  • KLF4
  • Kruppel-like factor 4 (gut)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KLF4 Antibody (NBP2-24749)(19)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, ISH, WB.


Filter By Application
ICC/IF
(1)
IHC
(4)
IHC-P
(6)
ISH
(1)
WB
(8)
All Applications
Filter By Species
Human
(12)
Mouse
(1)
Rat
(2)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NBP2-24749 Applications Species
Kilmister E, Patel J, Bockett N et al. Embryonic stem cell-like subpopulations are present within Schwannoma Journal of Clinical Neuroscience Nov 1 2020 [PMID: 33222917] (IHC, Human) IHC Human
Yoganandarajah V, Patel J et al. Identification of Cancer Stem Cell Subpopulations in Head and Neck Metastatic Malignant Melanoma. Cells Jan 30 2020 [PMID: 32019273] (IHC, WB, Human) IHC, WB Human
Zhou J, Liu S, Wang Y et al. Salinomycin effectively eliminates cancer stem-like cells and obviates hepatic metastasis in uveal melanoma Mol. Cancer Nov 13 2019 [PMID: 31718679] (WB, Human) WB Human
Zhu Y, Chen H, Zhao X et al. Decreased H3K9ac level of KLF4 mediates podocyte developmental toxicity induced by prenatal caffeine exposure in male offspring rats Toxicol. Lett. Jul 12 2019 [PMID: 31306741] (WB, Rat) WB Rat
Eady EK, Brasch HD, de Jongh J et al. Expression of Embryonic Stem Cell Markers in Microcystic Lymphatic Malformation Lymphat Res Biol Mar 22 2019 [PMID: 30901291] (IHC, Human) IHC Human
Yoganandarajah V, Patel J, van Schaijik B et al. Identification of Cancer Stem Cell Subpopulations in Head and Neck Metastatic Malignant Melanoma preprints 2019 Oct 21 (IHC-P, Human) IHC-P Human
Munro MJ, Peng L, Wickremesekera SK, Tan ST Colon adenocarcinoma-derived cells that express induced-pluripotent stem cell markers possess stem cell function PLoS ONE May 19 2020 [PMID: 32428045] (WB, Human) WB Human
Munro MJ, Wickremesekera SK, Peng L et al. Cancer stem cell subpopulations in primary colon adenocarcinoma PLoS ONE Sep 6 2019 [PMID: 31491003] (IHC, IHC-P, ISH, Human) IHC, IHC-P, ISH Human
Koh SP, Brasch HD, de Jongh J et al. Cancer stem cell subpopulations in moderately differentiated head and neck cutaneous squamous cell carcinoma Heliyon Aug 1 2019 [PMID: 31463389] (IHC-P, Human) IHC-P Human
Choi, H;Ryu, KY;Roh, J; Kruppel-like factor 4 plays a role in the luteal transition in steroidogenesis by down-regulating Cyp19A1 expression Am. J. Physiol. Endocrinol. Metab. Apr 2 2019 [PMID: 30939050] (IHC-P, Rat) IHC-P Rat
Show All 19 Publications.

Review for KLF4 Antibody (NBP2-24749) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-24749:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin KLF4 NBP2-24749
Enlarge
4
reviewed by:
Liyong Zhang
IHC-P Human 08/21/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
SpeciesHuman
Special ApplicationsBreast cancer tissues embedded in paraffin
FileView PDF

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for KLF4 Antibody (NBP2-24749) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for KLF4 Antibody (NBP2-24749)

Discover related pathways, diseases and genes to KLF4 Antibody (NBP2-24749). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for KLF4 Antibody (NBP2-24749)

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Blogs on KLF4.

Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness
By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax...  Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

KLF4 opens the door for stem cell research
KLF4 (Kruppel-like factor 4, Epithelial zinc finger protein EZF) is a zinc finger transcription factor thought to be involved in developmental differentiation and proliferation. It is considered a pluripotency reprogramming factor (PRF) due to its abi...  Read full blog post.

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Recent Reviews

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5
0
4
1
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0
2
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1
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Liyong Zhang
08/21/2014
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol KLF4
Uniprot