KLF5 Antibody [Unconjugated]

Images

 
Western blot shows lysates of BG01V human embryonic stem cells, D3 mouse embryonic stem cell line, and P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human KLF5 Antigen ...read more
Simple Western lane view shows lysates of D3 mouse embryonic stem cell line, loaded at 0.2 mg/mL. A specific band was detected for KLF5 at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and KLF5 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat ...read more
KLF5 was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Formulation Size Price

KLF5 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human KLF5
Leu85-Ala177
Accession # Q13887
Specificity
Detects human and mouse KLF5 in Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) KLF4, rhKLF6, recombinant mouse (rm) KLF4 and rmKLF15 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KLF5
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Knockout Validated
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3758 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KLF5 Antibody [Unconjugated]

  • Basic transcription element-binding protein 2
  • BTEB2
  • BTE-binding protein 2
  • CKLFGC-box-binding protein 2
  • Colon krueppel-like factor
  • colon kruppel-like factor
  • GC box binding protein 2
  • IKLFbasic transcription element binding protein 2
  • Intestinal-enriched krueppel-like factor
  • intestinal-enriched kruppel-like factor
  • KLF5
  • Krueppel-like factor 5
  • Kruppel-like factor 5 (intestinal)
  • Transcription factor BTEB2

Background

Human KLF5, also known as basic transcription element binding protein 2 (BTEB2), is a widely expressed 51 kDa (457 aa) member of the kruppel C2H2-type zinc finger protein family. KLF5 contains three C2H2-type zinc fingers at the carboxyl-terminus that preferentially bind to cis-DNA elements that are GC rich. It is a transcription activator that promotes cell proliferation and oncogenesis. Over the region used as immunogen, human, mouse and canine KLF5 share 95% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KLF5 Antibody (AF3758)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ChIP, ICC, IHC, Immunoprecipitation, Western Blot.


Filter By Application
ChIP
(2)
ICC
(2)
IHC
(3)
Immunoprecipitation
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(7)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF3758 Applications Species
Y Kong, W Ren, H Fang, NA Shah, Y Shi, D You, C Zhou, D Jiang, C Yang, H Liang, W Liu, L Wang, W Gan, X Wan, F Li, Z Li, C Chen, N Xie Histone Deacetylase Inhibitors (HDACi) Promote KLF5 Ubiquitination and Degradation in Basal-like Breast Cancer International journal of biological sciences, 2022;18(5):2104-2115. 2022 [PMID: 35342356] (Western Blot, Human) Western Blot Human
S Huang, P Kuri, Y Aubert, M Brewster, N Li, O Farrelly, G Rice, H Bae, S Prouty, T Dentchev, W Luo, BC Capell, P Rompolas Lgr6 marks epidermal stem cells with a nerve-dependent role in wound re-epithelialization Cell Stem Cell, 2021;0(0):. 2021 [PMID: 34102139] (IHC, Mouse) IHC Mouse
J Li, B Zhang, M Liu, X Fu, X Ci, J A, C Fu, G Dong, R Wu, Z Zhang, L Fu, JT Dong KLF5 Is Crucial for Androgen-AR Signaling to Transactivate Genes and Promote Cell Proliferation in Prostate Cancer Cells Cancers (Basel), 2020;12(3):. 2020 [PMID: 32245249] (ChIP, Human) ChIP Human
NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells Nat. Biotechnol., 2020;0(0):. 2020 [PMID: 32094658] (ICC, Human) ICC Human
Y Ge, Y Miao, S Gur-Cohen, N Gomez, H Yang, M Nikolova, L Polak, Y Hu, A Verma, O Elemento, JG Krueger, E Fuchs The aging skin microenvironment dictates stem cell behavior Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):. 2020 [PMID: 32094197] (IHC, Mouse) IHC Mouse
Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells Nat. Immunol., 2020;21(3):274-286. 2020 [PMID: 32066947] (ICC, Human) ICC Human
Y Wu, J Qin, F Li, C Yang, Z Li, Z Zhou, H Zhang, Y Li, X Wang, R Liu, Q Tao, W Chen, C Chen USP3 promotes breast cancer cell proliferation by deubiquitinating KLF5 J. Biol. Chem., 2019;0(0):. 2019 [PMID: 31624151]
R Tao, B Zhang, Y Li, JL King, R Tian, S Xia, CR Schiavon, JT Dong HDAC-mediated deacetylation of KLF5 associates with its proteasomal degradation Biochem. Biophys. Res. Commun., 2018;0(0):. 2018 [PMID: 29679567] (Immunoprecipitation, Human) Immunoprecipitation Human
Y Ge, NC Gomez, RC Adam, M Nikolova, H Yang, A Verma, CP Lu, L Polak, S Yuan, O Elemento, E Fuchs Stem Cell Lineage Infidelity Drives Wound Repair and Cancer Cell, 2017;0(0):. 2017 [PMID: 28434617] (IHC, Mouse) IHC Mouse
W Zhang, S Hong, KP Maniar, S Cheng, C Jie, AW Rademaker, AM Krensky, C Clayberger KLF13 regulates the differentiation-dependent human papillomavirus life cycle in keratinocytes through STAT5 and IL-8 Oncogene, 2016;0(0):. 2016 [PMID: 27041562] (Western Blot, Human) Western Blot Human
Show All 12 Publications.

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Product General Protocols

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WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for KLF5 Antibody (AF3758)

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Blogs on KLF5.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

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Bioinformatics

Gene Symbol KLF5
Uniprot