Western blot shows lysates of BG01V human embryonic stem cells, D3 mouse embryonic stem cell line, and P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human KLF5 Antigen ...read more
Simple Western lane view shows lysates of D3 mouse embryonic stem cell line, loaded at 0.2 mg/mL. A specific band was detected for KLF5 at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse ...read more
KLF5 was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and KLF5 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat ...read more
E. coli-derived recombinant human KLF5 Leu85-Ala177 Accession # Q13887
Detects human and mouse KLF5 in Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) KLF4, rhKLF6, recombinant mouse (rm) KLF4 and rmKLF15 is observed.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for KLF5 Antibody [Unconjugated]
Basic transcription element-binding protein 2
BTE-binding protein 2
CKLFGC-box-binding protein 2
Colon krueppel-like factor
colon kruppel-like factor
GC box binding protein 2
IKLFbasic transcription element binding protein 2
Intestinal-enriched krueppel-like factor
intestinal-enriched kruppel-like factor
Krueppel-like factor 5
Kruppel-like factor 5 (intestinal)
Transcription factor BTEB2
Human KLF5, also known as basic transcription element binding protein 2
(BTEB2), is a widely expressed 51 kDa (457 aa) member of the kruppel
C2H2-type zinc finger protein family. KLF5 contains three C2H2-type
zinc fingers at the carboxyl-terminus that preferentially bind to
cis-DNA elements that are GC rich. It is a transcription activator that
promotes cell proliferation and oncogenesis. Over the region used as
immunogen, human, mouse and canine KLF5 share 95% aa sequence identity.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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