KLF5 Antibody [Unconjugated]

Western blot shows lysates of BG01V human embryonic stem cells, D3 mouse embryonic stem cell line, and P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for KLF5 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

KLF5 was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of D3 mouse embryonic stem cell line, loaded at 0.2 mg/mL. A specific band was detected for KLF5 at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and KLF5 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for KLF5 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

KLF5 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human KLF5
  Leu85-Ala177
  Accession # Q13887
• Specificity: Detects human and mouse KLF5 in direct ELISAs. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) KLF4, rhKLF6, recombinant mouse (rm) KLF4 and rmKLF15 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KLF5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Knockout Validated
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KLF5 Antibody [Unconjugated]

• Basic transcription element-binding protein 2
• BTEB2
• BTE-binding protein 2
• CKLFGC-box-binding protein 2
• Colon krueppel-like factor
• colon kruppel-like factor
• GC box binding protein 2
• IKLFbasic transcription element binding protein 2
• Intestinal-enriched krueppel-like factor
• intestinal-enriched kruppel-like factor
• KLF5
• Krueppel-like factor 5
• Kruppel-like factor 5 (intestinal)
• Transcription factor BTEB2

Background

Human KLF5, also known as basic transcription element binding protein 2 (BTEB2), is a widely expressed 51 kDa (457 aa) member of the kruppel C2H2-type zinc finger protein family. KLF5 contains three C2H2-type zinc fingers at the carboxyl-terminus that preferentially bind to cis-DNA elements that are GC rich. It is a transcription activator that promotes cell proliferation and oncogenesis. Over the region used as immunogen, human, mouse and canine KLF5 share 95% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KLF5 Antibody (AF3758)(17)

Publications using AF3758

• Alavi, M;Mejia-Bautista, A;Tang, M;Bandovic, J;Rosenberg, AZ;Bialkowska, AB; Krüppel-like Factor 5 Plays an Important Role in the Pathogenesis of Chronic Pancreatitis Cancers 2023-11-15 [PMID: 38001687] (IHC, Mouse)
• P Zhao, J Sun, X Huang, X Zhang, X Liu, R Liu, G Du, W Gan, C Yang, Y Tang, C Chen, D Jiang Targeting the KLF5-EphA2 axis can restrain cancer stemness and overcome chemoresistance in basal-like breast cancer International journal of biological sciences, 2023-03-21;19(6):1861-1874. 2023-03-21 [PMID: 37063424] (Western Blot, ChIP, Human)
• Q Wu, Z Liu, Z Gao, Y Luo, F Li, C Yang, T Wang, X Meng, H Chen, J Li, Y Kong, C Dong, S Sun, C Chen KLF5 inhibition potentiates anti-PD1 efficacy by enhancing CD8+ T-cell-dependent antitumor immunity Theranostics, 2023-02-21;13(4):1381-1400. 2/21/2023 [PMID: 36923542]
• Keisuke Fukuchi, Kayoko Nanai, Hiroshi Yuita, Chikako Maru, Jun Tsukada, Masato Ishigami et al. Novel Antibody Exerts Antitumor Effect through Downregulation of CD147 and Activation of Multiple Stress Signals Journal of Oncology 11/4/2022 [PMID: 36385956]
• Y Kong, W Ren, H Fang, NA Shah, Y Shi, D You, C Zhou, D Jiang, C Yang, H Liang, W Liu, L Wang, W Gan, X Wan, F Li, Z Li, C Chen, N Xie Histone Deacetylase Inhibitors (HDACi) Promote KLF5 Ubiquitination and Degradation in Basal-like Breast Cancer International journal of biological sciences, 2022-02-28;18(5):2104-2115. 2022-02-28 [PMID: 35342356] (Western Blot, Human)
• S Huang, P Kuri, Y Aubert, M Brewster, N Li, O Farrelly, G Rice, H Bae, S Prouty, T Dentchev, W Luo, BC Capell, P Rompolas Lgr6 marks epidermal stem cells with a nerve-dependent role in wound re-epithelialization Cell Stem Cell, 2021-06-07;0(0):. 2021-06-07 [PMID: 34102139] (IHC, Mouse)
• J Li, B Zhang, M Liu, X Fu, X Ci, J A, C Fu, G Dong, R Wu, Z Zhang, L Fu, JT Dong KLF5 Is Crucial for Androgen-AR Signaling to Transactivate Genes and Promote Cell Proliferation in Prostate Cancer Cells Cancers (Basel), 2020-03-21;12(3):. 2020-03-21 [PMID: 32245249] (ChIP, Human)
• NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells Nat. Biotechnol., 2020-02-24;0(0):. 2020-02-24 [PMID: 32094658] (ICC, Human)
• Y Ge, Y Miao, S Gur-Cohen, N Gomez, H Yang, M Nikolova, L Polak, Y Hu, A Verma, O Elemento, JG Krueger, E Fuchs The aging skin microenvironment dictates stem cell behavior Proc. Natl. Acad. Sci. U.S.A., 2020-02-24;0(0):. 2020-02-24 [PMID: 32094197] (IHC, Mouse)
• Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells Nat. Immunol., 2020-02-17;21(3):274-286. 2020-02-17 [PMID: 32066947] (ICC, Human)
• CK Kim, M Saxena, K Maharjan, JJ Song, KR Shroyer, AB Bialkowska, RA Shivdasani, VW Yang Kr�ppel-like factor 5 regulates stemness, lineage specification, and regeneration of intestinal epithelial stem cells Cell Mol Gastroenterol Hepatol, 2019-11-25;0(0):. 11/25/2019 [PMID: 31778829]
• Y Wu, J Qin, F Li, C Yang, Z Li, Z Zhou, H Zhang, Y Li, X Wang, R Liu, Q Tao, W Chen, C Chen USP3 promotes breast cancer cell proliferation by deubiquitinating KLF5 J. Biol. Chem., 2019-10-17;0(0):. 2019-10-17 [PMID: 31624151] (Immunoprecipitation, Human)
• R Tao, B Zhang, Y Li, JL King, R Tian, S Xia, CR Schiavon, JT Dong HDAC-mediated deacetylation of KLF5 associates with its proteasomal degradation Biochem. Biophys. Res. Commun., 2018-04-24;0(0):. 2018-04-24 [PMID: 29679567] (Immunoprecipitation, Human)
• Y Ge, NC Gomez, RC Adam, M Nikolova, H Yang, A Verma, CP Lu, L Polak, S Yuan, O Elemento, E Fuchs Stem Cell Lineage Infidelity Drives Wound Repair and Cancer Cell, 2017-04-20;0(0):. 2017-04-20 [PMID: 28434617] (IHC, Mouse)
• W Zhang, S Hong, KP Maniar, S Cheng, C Jie, AW Rademaker, AM Krensky, C Clayberger KLF13 regulates the differentiation-dependent human papillomavirus life cycle in keratinocytes through STAT5 and IL-8 Oncogene, 2016-04-04;0(0):. 2016-04-04 [PMID: 27041562] (Western Blot, Human)
• Shin J, Carr A, Corner G, Togel L, Davaos-Salas M, Tran H, Chueh A, Al-Obaidi S, Chionh F, Ahmed N, Buchanan D, Young J, Malo M, Hodin R, Arango D, Sieber O, Augenlicht L, Dhillon A, Weber T, Mariadason J The intestinal epithelial cell differentiation marker intestinal alkaline phosphatase (ALPi) is selectively induced by histone deacetylase inhibitors (HDACi) in colon cancer cells in a Kruppel-like factor 5 (KLF5)-dependent manner. J Biol Chem, 2014-07-18;289(36):25306-16. 2014-07-18 [PMID: 25037223] (ChIP, Human)
• Biton M, Levin A, Slyper M, Alkalay I, Horwitz E, Mor H, Kredo-Russo S, Avnit-Sagi T, Cojocaru G, Zreik F, Bentwich Z, Poy MN, Artis D, Walker MD, Hornstein E, Pikarsky E, Ben-Neriah Y Epithelial microRNAs regulate gut mucosal immunity via epithelium-T cell crosstalk. Nat. Immunol., 2011-01-30;12(3):239-46. 2011-01-30 [PMID: 21278735] (IHC-P, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: KLF5
• Uniprot:
  • Q13887()