Western blot shows lysates of BG01V human embryonic stem cells, D3 mouse embryonic stem cell line, and P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human KLF5 Antigen ...read more
Simple Western lane view shows lysates of D3 mouse embryonic stem cell line, loaded at 0.2 mg/mL. A specific band was detected for KLF5 at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and KLF5 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat ...read more
KLF5 was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
E. coli-derived recombinant human KLF5 Leu85-Ala177 Accession # Q13887
Detects human and mouse KLF5 in Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) KLF4, rhKLF6, recombinant mouse (rm) KLF4 and rmKLF15 is observed.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for KLF5 Antibody [Unconjugated]
Basic transcription element-binding protein 2
BTE-binding protein 2
CKLFGC-box-binding protein 2
Colon krueppel-like factor
colon kruppel-like factor
GC box binding protein 2
IKLFbasic transcription element binding protein 2
Intestinal-enriched krueppel-like factor
intestinal-enriched kruppel-like factor
Krueppel-like factor 5
Kruppel-like factor 5 (intestinal)
Transcription factor BTEB2
Human KLF5, also known as basic transcription element binding protein 2
(BTEB2), is a widely expressed 51 kDa (457 aa) member of the kruppel
C2H2-type zinc finger protein family. KLF5 contains three C2H2-type
zinc fingers at the carboxyl-terminus that preferentially bind to
cis-DNA elements that are GC rich. It is a transcription activator that
promotes cell proliferation and oncogenesis. Over the region used as
immunogen, human, mouse and canine KLF5 share 95% aa sequence identity.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.