KA2/GRIK5/Glutamate Receptor KA2 Antibody (2916E) [Unconjugated]

KA2/GRIK5 was detected in immersion fixed paraffin-embedded sections of Human Brain Hippocampus using Rabbit Anti-Human KA2/GRIK5 Monoclonal Antibody (Catalog # MAB113341) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 2916E
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

KA2/GRIK5/Glutamate Receptor KA2 Antibody (2916E) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide sequence
  Accession # Q16478
• Specificity: Detects human KA2/GRIK5 in direct ELISA.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KA2/GRIK5/Glutamate Receptor KA2 Antibody (2916E) [Unconjugated]

• EAA2
• Excitatory amino acid receptor 2
• GluK5
• glutamate receptor KA2
• Glutamate receptor KA-2
• glutamate receptor, ionotropic kainate 5
• glutamate receptor, ionotropic, kainate 5
• GRIK2
• GRIK5
• KA2/GRIK5
• KA2GRIK2

Background

GRIK5 (Glutamate receptor, ionotropic kainate 5) is a receptor for glutamate and is one of the 5 types of kainate receptor subunits.  L-Glutamate acts as an excitatory neurotransmitter at many synapses in the CNS. The GRIK5 protein forms functional heteromic kainate-preferring ionic channels with the subunits encoded by related gene family members.  Kainate receptors have both presynaptic and postsynaptic actions and a limited distribution in the brain. Kainate receptors have been shown to have connections with several neurological diseases and conditions.  There is linkage to schizophrenia, depression, autism, Huntington's, bipolar disorder, and epilepsy among others.  KA2 is relatively abundant in all areas of the brain, most notably in dentate gyrus, pyramidal neurons of CA3, cerebellar granule cells, as well as being present in superficial and deep laminae of the neocortex.

1. Entrez Gene: GRIK5 glutamate receptor, ionotropic, kainate 5.
2. Szpirer C, Molne M, Antonacci R, Jenkins NA, Finelli P, Szpirer J, Riviere M, Gilbert DJ, Copeland NG, et al. "The Genese Encoding the Glutamate Receptor Subunits Ka1 and KA2 (GRIK4 and GRIK5) are located on separate chromosomes in Human, Mouse and Rat". Proc Natl Acad Sci U.S.A. 1995 Jan; 91(25):11849.
3. Contractor A, Mulle C, Swanson GT. "Kainate Receptors Coming of Age: Milestones of Two Decades of Research". Trends in Neurosciences. 2011 Mar; 34(3):154.
4. Matute C. "Therapeutic Potential of Kainate Receptors". CNS Neuroscience & Therapeutics. 2011 Dec; 17(6):661.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q16478(Human)