mGluR5/1 Antibody

Images

 
Western Blot: metabotropic Glutamate Receptor 5/1 Antibody [NB300-126] - 10 ug of HEK 293 cells expression. As shown in the autoradiograph, the mGluR5/1a antibody recognizes both mGluR5 (Lane A) and mGluR1a (Lane B) ...read more

Product Details

Summary
Reactivity Mu, Rt, HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

mGluR5/1 Antibody Summary

Immunogen
Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
Localization
Cell Membrane
Specificity
Specific for the ~125k monomer and the ~250k dimers of mGluR5 and mGluR1. Immunolabeling is blocked by preadsorption of antibody with the peptide used as antigen to generate the antibody.
Predicted Species
Human (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GRM5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:500
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30868621).
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB300-126 in the following applications:

Publications
Read Publications using
NB300-126 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 15608650).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for mGluR5/1 Antibody

  • glutamate receptor, metabotropic 5
  • GPRC1EMGLUR5metabotropic glutamate receptor 5
  • mGlu5
  • mGluR5

Background

Glutamate is the main excitatory neurotransmitter in the brain. For many years it had been considered to act only on the ligand-gated receptor channels-termed NMDA, AMPA and kainate receptors that are involved in the fast excitatory synaptic transmission. Recently, glutamate has been shown to regulate enzymes producing second messengers via specific receptors coupled to G-proteins. These receptors are called metabotropic glutamate receptors. In expression systems, Group-I receptors, stimulate phospholipase C as revealed by an increase in phosphoinositide turnover, and Ca2+ release from internal stores. Group-II and -III receptors are coupled to the inhibition of adenylyl cyclase. The Group-I receptors include mGluR1a and mGluR5. The Group-II receptors in include mGluR2 and mGluR3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for mGluR5/1 Antibody (NB300-126)(3)

Review for mGluR5/1 Antibody (NB300-126) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB300-126:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot mGluR5/1 NB300-126
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5
reviewed by:
Daniel Popoola
WB Rat 03/21/2017
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Summary

ApplicationWestern Blot
Sample Testedbrain
SpeciesRat

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Array NB300-126

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Recent Reviews

5
5
1
4
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3
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2
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1
0

Daniel Popoola
03/21/2017
Application: WB
Species: Rat

Bioinformatics

Gene Symbol GRM5
Entrez
Uniprot