mGluR5/1 Antibody

Western Blot: metabotropic Glutamate Receptor 5/1 Antibody [NB300-126] - 10 ug of HEK 293 cells expression. As shown in the autoradiograph, the mGluR5/1a antibody recognizes both mGluR5 (Lane A) and mGluR1a (Lane B) labeling the ~125k monomer and the ~250k dimers for both mGluRs.

• Reactivity: Mu, Rt, Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

mGluR5/1 Antibody Summary

• Immunogen: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Accession # P31424
• Localization: Cell Membrane
• Specificity: Specific for endogenous levels of the ~125 kDa monomer and the ~250 kDa dimers of mGluR5 and mGluR1. Immunolabeling blocked by preadsorption of antibody with the peptide used to generate the antibody.
• Predicted Species: Human (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRM5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:500
  • Western Blot 1:1000
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30868621).
• Theoretical MW: 125 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 15608650).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for mGluR5/1 Antibody

• glutamate receptor, metabotropic 5
• GPRC1EMGLUR5metabotropic glutamate receptor 5
• mGlu5
• mGluR5

Background

Glutamate is the main excitatory neurotransmitter in the brain. For many years it had been considered to act only on the ligand-gated receptor channels-termed NMDA, AMPA and kainate receptors that are involved in the fast excitatory synaptic transmission. Recently, glutamate has been shown to regulate enzymes producing second messengers via specific receptors coupled to G-proteins. These receptors are called metabotropic glutamate receptors. In expression systems, Group-I receptors, stimulate phospholipase C as revealed by an increase in phosphoinositide turnover, and Ca2+ release from internal stores. Group-II and -III receptors are coupled to the inhibition of adenylyl cyclase. The Group-I receptors include mGluR1a and mGluR5. The Group-II receptors in include mGluR2 and mGluR3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mGluR5/1 Antibody (NB300-126)(3)

Publications using NB300-126

• Lee K, Vyas Y, Garner CC, Montgomery JM. Autism-associated Shank3 mutations alter mGluR expression and mGluR-dependent but not NMDA receptor-dependent long-term depression Synapse 2019-03-13 [PMID: 30868621] (ICC/IF, Rat)
• Cheyne JE, Grant L, Butler-Munro C et al. Synaptic integration of newly generated neurons in rat dissociated hippocampal cultures. Mol Cell Neurosci 2011-07-01 [PMID: 21569851] (Rat)
• Spanagel, R et al. The clock gene Per2 influences the glutamatergic system modulates alchol consumption. Nature Medicine2005. [PMID: 15608650] (WB, Mouse)

Review for mGluR5/1 Antibody (NB300-126) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer WB Rat 03/21/2017

Summary

• Application: Western Blot
• Sample Tested: brain
• Species: Rat

Bioinformatics

• Gene Symbol: GRM5
• Entrez:
  • 24418(Rat)
• Uniprot:
  • P31424(Rat)