NMDAR1 Antibody (R1JHL)

Images

 
Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118]
Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - Mouse neuron stained with anti-NMDAR1 antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC-Fr, IHC-P, IP, IF
Clone
R1JHL
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

NMDAR1 Antibody (R1JHL) Summary

Immunogen
Fusion protein containing amino acids 1-564 of the NR1 subunit of rat NMDA receptor.
Marker
Neuronal Marker
Specificity
Specific for the ~120k NR1 subunit of the NMDA receptor.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
GRIN1
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen 1 ug per ml
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 3ug/200ug lysate
  • Immunofluorescence
Application Notes
Use in Immunocytochemistry/immunofluorescence and Immunohistochemistry-Frozen reported in scientific literature (PMID: 20414717). Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 26635527).
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 14 Reviews rated 4.3
using
NB300-118 in the following applications:

Publications
Read Publications using
NB300-118 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 20414717). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Culture supernatant
Preservative
No Preservative
Concentration
LYOPH
Purity
Tissue culture supernatant
Reconstitution Instructions
Reconstitute with 50 ul PBS to desired concetration.

Alternate Names for NMDAR1 Antibody (R1JHL)

  • glutamate [NMDA] receptor subunit zeta-1
  • glutamate receptor, ionotropic, N-methyl D-aspartate 1
  • NMDA1
  • NMDAR1glutamate [NMDA] receptor subunit zeta 1
  • NMD-R1
  • N-methyl-D-aspartate receptor channel, subunit zeta-1
  • N-methyl-D-aspartate receptor subunit NR1
  • NR1

Background

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC-Fr, IHC-P, IP, IF

Publications for NMDAR1 Antibody (NB300-118)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IHC-Fr
(1)
IHC-P
(1)
WB
(6)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
Rat
(6)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB300-118 Applications Species
Gorska-Ponikowska M, Perricone U, Kuban-Jankowska A et al. 2-Methoxyestradiol Impacts on Amino Acids-mediated Metabolic Reprogramming in Osteosarcoma Cells by Interaction with NMDA Receptor. J. Cell. Physiol. Mar 6 2017 [PMID: 28262924] (WB, Human) WB Human
Hewett SJ, Shi J, Gong Y et al. Spontaneous Glutamatergic Synaptic Activity Regulates Constitutive COX-2 Expression in Neurons: Opposing roles for the transcription factors CREB and Sp1. J. Biol. Chem. Nov 14 2016 [PMID: 27875294] (WB, Mouse) WB Mouse
Brai E, Marathe S, Astori S et al. Notch1 Regulates Hippocampal Plasticity Through Interaction with the Reelin Pathway, Glutamatergic Transmission and CREB Signaling. Front Cell Neurosci. 2015 Dec 04 [PMID: 26635527] (IHC-P, Mouse) IHC-P Mouse
Plaza-Zabala A, Li X, Milovanovic M et al. An investigation of interactions between hypocretin/orexin signaling and glutamate receptor surface expression in the rat nucleus accumbens under basal conditions. Neurosci Lett. 2013 Dec 17 [PMID: 24262606] (WB, Rat) WB Rat
Tao P-L, Chen C-F, Huang EY-K. Dextromethorphan attenuated the higher vulnerability to inflammatory thermal hyperalgesia caused by prenatal morphine exposure in rat offspring. J Biomed Sci;18(1):64. 2011 Aug 23. [PMID: 21861871] (WB, Rat) WB Rat
Ferrario CR, Loweth JA, Milovanovic M et al. Distribution of AMPA receptor subunits and TARPs in synaptic and extrasynaptic membranes of the adult rat nucleus accumbens Neurosci Lett 2011 Mar [PMID: 21182898] (WB, Rat) WB Rat
Mashkina AP, Cizkova D, Vanicky I et al. NMDA receptors are expressed in lymphocytes activated both in vitro and in vivo. Cell Mol Neurobiol 2010 Aug [PMID: 20414717] (IHC-Fr, ICC/IF, Rat, Human) IHC-Fr, ICC/IF Rat, Human
Garraway SM, Xu Q, Inturrisi CE. siRNA-mediated knockdown of the NR1 subunit gene of the NMDA receptor attenuates formalin-induced pain behaviors in adult rats. J Pain 2009 Apr [PMID: 19185544] (Rat) Rat
Lim, G et al. Expression of spinal NMDA receptor PKC after chronic morphine is regulated by spinal glucorticoid receptor, J. Neurosci2005. [PMID: 16319314] (ICC/IF, WB, Rat) ICC/IF, WB Rat
Nagy, J et al. Differential alterations in the expression of NMDA recaptor subunits following chromic ethanol treatment in primary cultures of rat cortical hippocampal neurons. Neurochemistry International, 42(2003):35-43. 2002 [PMID: 12441166]

Reviews for NMDAR1 Antibody (NB300-118) (14) 4.314

Average Rating: 4.3
(Based on 14 reviews)
We have 14 reviews tested in 3 species: Human, Mouse, Rat.
We have 14 reviews tested in 4 application: ICC, IP, WB, IF.

Reviews using NB300-118:
Filter by Applications
ICC
(4)
IP
(5)
WB
(3)
IF
(2)
All Applications
Filter by Species
Human
(3)
Mouse
(10)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry NMDAR1 NB300-118
Enlarge
reviewed by:
Anonymous
ICC Mouse 08/04/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedCultured hippocampal neurons
SpeciesMouse
LotBW1016c
Immunoprecipitation NMDAR1 NB300-118
Enlarge
reviewed by:
Anonymous
IP Mouse 08/04/2017
View

Summary

ApplicationImmunoprecipitation
Sample TestedMice Brain Lysate
SpeciesMouse
LotBW1016c
Western Blot NMDAR1 NB300-118
Enlarge
reviewed by:
Anonymous
WB Human 08/04/2017
View

Summary

ApplicationWestern Blot
Sample Tested293T
SpeciesHuman
LotWB1016c
Immunoprecipitation NMDAR1 NB300-118
Enlarge
reviewed by:
Anonymous
IP Mouse 07/14/2017
View

Summary

ApplicationImmunoprecipitation
Sample Tested brain and spinal cord
SpeciesMouse
LotBW1016c
Immunocytochemistry NMDAR1 NB300-118
Enlarge
reviewed by:
Anonymous
ICC Mouse 07/14/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedCortical neurons
SpeciesMouse
LotBW1016c
Western Blot NMDAR1 NB300-118
Enlarge
reviewed by:
Anonymous
WB Human 07/14/2017
View

Summary

ApplicationWestern Blot
Sample Tested brain and spinal cord
SpeciesHuman
LotBW1016c
Immunocytochemistry NMDAR1 NB300-118
Enlarge
reviewed by:
Janosch Heller
ICC Rat 06/27/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedrat cortical culture (3 weeks old)
SpeciesRat

Comments

CommentsFixation Solution and Conditions: 4% paraformaldehyde in PBS, 15 minutes RT

Blocking Solution & Duration: 3% BSA in PBS with 0.2% saponin, 30 minutes RT

Primary Antibody Diluent and Dilutions Tested: 1:200 in 1% BSA in PBS with 0.2% saponin, over night 4°C

Secondary Antibody Manufacturer, Host Species, Dilution, & Diluent: Thermo donkey anti-mouse CF568, 1:250 in PBS with 0.2% saponin, 1 hour, RT
Western Blot NMDAR1 NB300-118
Enlarge
reviewed by:
Anonymous
WB Mouse 11/26/2015
View

Summary

ApplicationWestern Blot
Sample TestedMouse Brain Tissue
SpeciesMouse

Blocking

Blocking Details5% skimmilk in TBS-T

Secondary Antibody

Secondary Descriptionanti-mouse IgG, HRP

Details

Detection NotesWestern blot
Immunofluorescence NMDAR1 NB300-118
Enlarge
reviewed by:
Anonymous
IF Mouse 11/26/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse neurons
SpeciesMouse

Blocking

Blocking Details1% BSA

Details

Detection NotesConfocal microscopy
Immunocytochemistry NMDAR1 NB300-118
Enlarge
reviewed by:
Anonymous
ICC Mouse 11/26/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse neurons
SpeciesMouse
LotBW714r

Blocking

Blocking Details1% BSA for 1 hr

Details

Detection NotesConfocal microscopy
Immunoprecipitation NMDAR1 NB300-118
Enlarge
reviewed by:
Anonymous
IP Mouse 10/16/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedMouse Brain Tissue
SpeciesMouse
LotBW714r

Blocking

Blocking Details5% skimmilk in TBS-T

Details

Detection NotesChemiluminescence detection
Immunoprecipitation NMDAR1 NB300-118
Enlarge
reviewed by:
Anonymous
IP Mouse 10/15/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedMouse neuronal cultures
SpeciesMouse
LotBW714r

Blocking

Blocking Details5%skimmilk in TBS-T

Details

Detection NotesChemiluminescence
Immunoprecipitation NMDAR1 NB300-118
Enlarge
reviewed by:
Anonymous
IP Mouse 10/15/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedMouse neurons
SpeciesMouse
LotBW714r

Blocking

Blocking Details5% skimmilk in TBS-T

Details

Detection NotesChemiluminescence, 5 min using West Pico, whole cell lysate as positive and non-immune IgG as negative contril
Immunoprecipitation DetailsProtein G
 
reviewed by:
Fugui Li
IF Human 09/30/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedNR1 plasmid transfected HEK293T cells
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NMDAR1 Antibody (NB300-118) (0)

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Recent Reviews

4.3
5
5
4
8
3
1
2
0
1
0

Anonymous
08/04/2017
Application: ICC
Species: Mouse

Anonymous
08/04/2017
Application: IP
Species: Mouse

Anonymous
08/04/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol GRIN1
Entrez
Uniprot