NMDAR1 Antibody (R1JHL)

Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor.

Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - The blots show the examples of immunobands against NMDAR1 and beta-actin antibodies on the membrane, which was performed on the membrane protein prepared from the lumbar spinal cords of the offspring (P14) rats in different groups. Image collected and cropped by CiteAb from the following publication (//www.jbiomedsci.com/content/18/1/64) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - NR1 surface staining of mouse corical neruons. This image was submitted via customer Review.

Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - Mouse neuron stained with anti-NMDAR1 antibody. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - Mixed rat cortical cultures (21 DIV) were labelled with anti-NMDAR1 (1:200) and anti-mouse CF568 (1:250). The antibody labels small punctae along the neuronal processes. No distinct features such as spines are visible. This image was submitted via customer Review.

Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (R1JHL) - Azide and BSA Free [NB300-118] - Notch1 colocalizes postsynaptically with Reelin signaling components. (A) Representative IEM images from hippocampal slices using an antibody specific for Notch1 show that gold particles are localized at postsynaptic as well as presynaptic membrane terminals. (B) Bar graph summarizing the counting of gold particles on the length of presynaptic & postsynaptic membranes indicates that the majority of the Notch1 gold particles are localized postsynaptically (5*10−3 ± 0.8*10−3 vs. 2*10−3 ± 0.4*10−3, n = 3 mice; Student’s t-test, p < 0.01). (C) Fluorescent immunolabeling on 14 days primary neuronal WT cultures shows colocalization of Notch1 & ApoER2 in soma & processes of pyramidal neurons. Both Notch1 & ApoER2 are similarly co-expressed in NMDAR1 positive puncta (R = 0.92 ± 0.03 & R = 0.86 ± 0.08, respectively; Student’s t-test, p = 0.23). (C′) Close up of a dendrite displaying Notch1, ApoER2 & NMDAR1 expression & “zoom in” captions of puncta showing clustering of the three receptors in teal. (D) Fluorescent immunolabeling on primary neuronal cultures shows that Notch1 & Dab1 localize in the same pyramidal neuron’s soma & processes labeled by phalloidin (R = 0.87 ± 0.06 & R = 0.74 ± 0.07 respectively; Student’s t-test, p = 0.5). (D′) Close up of a dendrite with Notch1, Dab1 & F-actin labeling & “zoom in” captions of dendritic puncta showing clustering of Notch1, Dab1 & F-actin in teal. **p < 0.01. Error bars are SEM & scale bar in (A) is 200 nm for all IEM panels, in (C,D) 50 μm & in (C′,D′) 10 μm & 500 nm in zoom in captions. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26635527), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: R1JHL
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NMDAR1 Antibody (R1JHL) Summary

• Description: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
• Immunogen: Fusion protein containing amino acids 1-564 of the NMDAR1 subunit. Accession # P35439
• Marker: Neuronal Marker
• Specificity: Specific for endogenous levels of the ~120 kDa NR1 subunit of the NMDA receptor.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRIN1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1 ug per ml
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Application Notes: Use in ICC/IF and IHC-F reported in scientific literature (PMID: 20414717). Use in IHC-P reported in scientific literature (PMID 26635527).
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 20414717). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Tissue culture supernatant
• Reconstitution Instructions: Reconstitute with 50 ul PBS to desired concentration.

Alternate Names for NMDAR1 Antibody (R1JHL)

• glutamate [NMDA] receptor subunit zeta-1
• glutamate receptor, ionotropic, N-methyl D-aspartate 1
• NMDA1
• NMDAR1glutamate [NMDA] receptor subunit zeta 1
• NMD-R1
• N-methyl-D-aspartate receptor channel, subunit zeta-1
• N-methyl-D-aspartate receptor subunit NR1
• NR1

Background

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMDAR1 Antibody (NB300-118)(22)

Publications using NB300-118

• Hossen E, Funahashi Y, Faruk M et al. Rho-Kinase/ROCK Phosphorylates PSD-93 Downstream of NMDARs to Orchestrate Synaptic Plasticity International Journal of Molecular Sciences 2022-12-26 [PMID: 36613848] (WB, Mouse)
• Perri RGB, Mantello AG, Rosa DS, Beleboni RO. Silencing of the GluN1-NMDA Glutamate Receptor Subunit by Intranasal siRNA Increases the Latency Time for Seizures in the Pilocarpine Rodent Model of Epilepsy Pharmaceuticals (Basel) 2022-11-26 [PMID: 36558924]
• Yamahashi Y, Lin YH, Mouri A et al. Phosphoproteomic of the acetylcholine pathway enables discovery of the PKC-beta-PIX-Rac1-PAK cascade as a stimulatory signal for aversive learning Molecular psychiatry 2022-06-03 [PMID: 35665767] (WB)
• Rahim M, Yoon Y, Dimovasili C et al. PS1 familial Alzheimer disease mutants inactivate EFNB1- and BDNF-dependent neuroprotection against excitotoxicity by affecting neuroprotective complexes of NMDA receptor Brain Commun 2020-10-02 [PMID: 33005890]
• Wang, Y, Kerrisk Campbell, M Et al. PCDH7 interacts with GluN1 and regulates dendritic spine morphology and synaptic function. Sci Rep 2020-07-02 [PMID: 32616769] (FLOW, Rat)
  
  Details:
  Citation using the Alexa Fluor 647 format of this antibody.
• Staats KA, Seah C, Sahimi A, Wang Y Small molecule inhibition of PIKFYVE kinase rescues gain-and loss-of-function C9ORF72 ALS/FTD disease processes in vivo bioRxiv 2019-06-28 (WB, Mouse)
• Lin YH, Yamahashi Y, Kuroda K, Faruk MO et Al. Accumbal D2R-medium spiny neurons regulate aversive behaviors through PKA-Rap1 pathway Neurochem Int 2020-12-10 [PMID: 33301817]
• Shi Y, Hung ST, Rocha G et al. Identification and therapeutic rescue of autophagosome and glutamate receptor defects in C9ORF72 and sporadic ALS neurons JCI Insight 2019-07-16 [PMID: 31310593] (WB, Human)
• Stewart M, Lau P, Banks G et al. Loss of Frrs1l disrupts synaptic AMPA receptor function, and results in neurodevelopmental, motor, cognitive and electrographical abnormalities Dis Model Mech 2019-02-22 [PMID: 30692144] (WB, Mouse)
• Stewart ME. Development of high-throughput, non-invasive behavioural and cognitive tests in mice to uncover new mechanisms of abnormal cognition and behaviour Thesis 1905-07-10 (Mouse)
• Mireia Carcolé, Sami Kummer, Leonor Gonçalves, Daniel Zamanillo, Manuel Merlos, Anthony H. Dickenson, Begoña Fernández‐Pastor, David Cabañero, Rafael Maldonado Sigma‐1 receptor modulates neuroinflammation associated with mechanical hypersensitivity and opioid tolerance in a mouse model of osteoarthritis pain British Journal of Pharmacology 2019-09-12 [PMID: 31332781]
• Shi Y, Lin S, Staats KA, Li Y. Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons. Nat. Med. 2018-02-05 [PMID: 29400714] (Human)
• Gorska-Ponikowska M, Perricone U, Kuban-Jankowska A et al. 2-Methoxyestradiol Impacts on Amino Acids-mediated Metabolic Reprogramming in Osteosarcoma Cells by Interaction with NMDA Receptor. J. Cell. Physiol. 2017-03-06 [PMID: 28262924] (WB, Human)
• Hewett SJ, Shi J, Gong Y et al. Spontaneous Glutamatergic Synaptic Activity Regulates Constitutive COX-2 Expression in Neurons: Opposing roles for the transcription factors CREB and Sp1. J. Biol. Chem. 2016-11-14 [PMID: 27875294] (WB, Mouse)
• Brai E, Marathe S, Astori S et al. Notch1 Regulates Hippocampal Plasticity Through Interaction with the Reelin Pathway, Glutamatergic Transmission and CREB Signaling. Front Cell Neurosci. 2015-12-04 [PMID: 26635527] (IHC-P, Mouse)
• Plaza-Zabala A, Li X, Milovanovic M et al. An investigation of interactions between hypocretin/orexin signaling and glutamate receptor surface expression in the rat nucleus accumbens under basal conditions. Neurosci Lett. 2013-12-17 [PMID: 24262606] (WB, Rat)
• Tao P-L, Chen C-F, Huang EY-K. Dextromethorphan attenuated the higher vulnerability to inflammatory thermal hyperalgesia caused by prenatal morphine exposure in rat offspring. J Biomed Sci;18(1):64. 2011-08-23 [PMID: 21861871] (WB, Rat)
• Ferrario CR, Loweth JA, Milovanovic M et al. Distribution of AMPA receptor subunits and TARPs in synaptic and extrasynaptic membranes of the adult rat nucleus accumbens Neurosci Lett 2011-03-01 [PMID: 21182898] (WB, Rat)
• Mashkina AP, Cizkova D, Vanicky I et al. NMDA receptors are expressed in lymphocytes activated both in vitro and in vivo. Cell Mol Neurobiol 2010-08-01 [PMID: 20414717] (IHC-Fr, ICC/IF, Rat, Human)
• Garraway SM, Xu Q, Inturrisi CE. siRNA-mediated knockdown of the NR1 subunit gene of the NMDA receptor attenuates formalin-induced pain behaviors in adult rats. J Pain 2009-04-01 [PMID: 19185544] (Rat)
• Lim, G et al. Expression of spinal NMDA receptor PKC after chronic morphine is regulated by spinal glucorticoid receptor, J. Neurosci2005. [PMID: 16319314] (WB, ICC/IF, Rat)
• Nagy, J et al. Differential alterations in the expression of NMDA recaptor subunits following chromic ethanol treatment in primary cultures of rat cortical hippocampal neurons. Neurochemistry International, 42(2003):35-43. 2002-01-01 [PMID: 12441166]

Reviews for NMDAR1 Antibody (NB300-118) (14)

Average Rating: 4.2

(Based on 14 reviews)

We have 14 reviews tested in 3 species: Human, Mouse, Rat.

reviewed by: Verified Customer WB Mouse 01/31/2018

Summary

• Application: Western Blot
• Sample Tested: Mouse brain
• Species: Mouse
• Lot: BW1017c

reviewed by: Verified Customer ICC Mouse 08/04/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Cultured hippocampal neurons
• Species: Mouse
• Lot: BW1016c

reviewed by: Verified Customer IP Mouse 08/04/2017

Summary

• Application: Immunoprecipitation
• Sample Tested: Mice Brain Lysate
• Species: Mouse
• Lot: BW1016c

reviewed by: Verified Customer WB Human 08/04/2017

Summary

• Application: Western Blot
• Sample Tested: 293T
• Species: Human
• Lot: WB1016c

reviewed by: Verified Customer IP Mouse 07/14/2017

Summary

• Application: Immunoprecipitation
• Sample Tested: brain and spinal cord
• Species: Mouse
• Lot: BW1016c

reviewed by: Verified Customer ICC Mouse 07/14/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Cortical neurons
• Species: Mouse
• Lot: BW1016c

reviewed by: Verified Customer WB Human 07/14/2017

Summary

• Application: Western Blot
• Sample Tested: brain and spinal cord
• Species: Human
• Lot: BW1016c

reviewed by: Janosch Heller ICC Rat 06/27/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: rat cortical culture (3 weeks old)
• Species: Rat

Comments

• Comments: Fixation Solution and Conditions: 4% paraformaldehyde in PBS, 15 minutes RT
  
  Blocking Solution & Duration: 3% BSA in PBS with 0.2% saponin, 30 minutes RT
  
  Primary Antibody Diluent and Dilutions Tested: 1:200 in 1% BSA in PBS with 0.2% saponin, over night 4°C
  
  Secondary Antibody Manufacturer, Host Species, Dilution, & Diluent: Thermo donkey anti-mouse CF568, 1:250 in PBS with 0.2% saponin, 1 hour, RT

reviewed by: Verified Customer IF Mouse 11/26/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse neurons
• Species: Mouse

reviewed by: Verified Customer ICC Mouse 11/26/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse neurons
• Species: Mouse
• Lot: BW714r

reviewed by: Verified Customer IP Mouse 10/16/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: Mouse Brain Tissue
• Species: Mouse
• Lot: BW714r

reviewed by: Verified Customer IP Mouse 10/15/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: Mouse neuronal cultures
• Species: Mouse
• Lot: BW714r

reviewed by: Verified Customer IP Mouse 10/15/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: Mouse neurons
• Species: Mouse
• Lot: BW714r

reviewed by: Fugui Li IF Human 09/30/2014

Summary

• Application: Immunofluorescence
• Sample Tested: NR1 plasmid transfected HEK293T cells
• Species: Human

Bioinformatics

• Gene Symbol: GRIN1
• Entrez:
  • 24408(Rat)
• Uniprot:
  • P35439(Rat)