NMDAR1 Antibody (R1JHL)

Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor.

Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - NR1 surface staining of mouse corical neruons. This image was submitted via customer Review.

Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - Mouse neuron stained with anti-NMDAR1 antibody. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - Mixed rat cortical cultures (21 DIV) were labelled with anti-NMDAR1 (1:200) and anti-mouse CF568 (1:250). The antibody labels small punctae along the neuronal processes. No distinct features such as spines are visible. This image was submitted via customer Review.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC-Fr, IHC-P, IP
• Clone: R1JHL
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NMDAR1 Antibody (R1JHL) Summary

• Immunogen: Fusion protein containing amino acids 1-564 of the NR1 subunit of rat NMDA receptor.
• Marker: Neuronal Marker
• Specificity: Specific for the ~120k NR1 subunit of the NMDA receptor.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRIN1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen 1 ug per ml
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 3ug/200ug lysate
• Application Notes: Use in ICC/IF and IHC-F reported in scientific literature (PMID: 20414717). Use in IHC-P reported in scientific literature (PMID 26635527).
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NMDAR1 Knockout HeLa Cell Lysate

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 20414717). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Tissue culture supernatant
• Reconstitution Instructions: Reconstitute with 50 ul PBS to desired concentration.

Alternate Names for NMDAR1 Antibody (R1JHL)

• glutamate [NMDA] receptor subunit zeta-1
• glutamate receptor, ionotropic, N-methyl D-aspartate 1
• NMDA1
• NMDAR1glutamate [NMDA] receptor subunit zeta 1
• NMD-R1
• N-methyl-D-aspartate receptor channel, subunit zeta-1
• N-methyl-D-aspartate receptor subunit NR1
• NR1

Background

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMDAR1 Antibody (NB300-118)(13)

Publications using NB300-118

• Stewart M, Lau P, Banks G et al. Loss of Frrs1l disrupts synaptic AMPA receptor function, and results in neurodevelopmental, motor, cognitive and electrographical abnormalities Dis Model Mech Feb 22 2019 [PMID: 30692144] (WB, Mouse)
• Shi Y, Lin S, Staats KA, Li Y. Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons. Nat. Med. 2018 Feb 05 [PMID: 29400714] (Human)
• Gorska-Ponikowska M, Perricone U, Kuban-Jankowska A et al. 2-Methoxyestradiol Impacts on Amino Acids-mediated Metabolic Reprogramming in Osteosarcoma Cells by Interaction with NMDA Receptor. J. Cell. Physiol. Mar 6 2017 [PMID: 28262924] (WB, Human)
• Hewett SJ, Shi J, Gong Y et al. Spontaneous Glutamatergic Synaptic Activity Regulates Constitutive COX-2 Expression in Neurons: Opposing roles for the transcription factors CREB and Sp1. J. Biol. Chem. Nov 14 2016 [PMID: 27875294] (WB, Mouse)
• Brai E, Marathe S, Astori S et al. Notch1 Regulates Hippocampal Plasticity Through Interaction with the Reelin Pathway, Glutamatergic Transmission and CREB Signaling. Front Cell Neurosci. 2015 Dec 04 [PMID: 26635527] (IHC-P, Mouse)
• Plaza-Zabala A, Li X, Milovanovic M et al. An investigation of interactions between hypocretin/orexin signaling and glutamate receptor surface expression in the rat nucleus accumbens under basal conditions. Neurosci Lett. 2013 Dec 17 [PMID: 24262606] (WB, Rat)
• Tao P-L, Chen C-F, Huang EY-K. Dextromethorphan attenuated the higher vulnerability to inflammatory thermal hyperalgesia caused by prenatal morphine exposure in rat offspring. J Biomed Sci;18(1):64. 2011 Aug 23. [PMID: 21861871] (WB, Rat)
• Ferrario CR, Loweth JA, Milovanovic M et al. Distribution of AMPA receptor subunits and TARPs in synaptic and extrasynaptic membranes of the adult rat nucleus accumbens Neurosci Lett 2011 Mar [PMID: 21182898] (WB, Rat)
• Mashkina AP, Cizkova D, Vanicky I et al. NMDA receptors are expressed in lymphocytes activated both in vitro and in vivo. Cell Mol Neurobiol 2010 Aug [PMID: 20414717] (IHC-Fr, ICC/IF, Rat, Human)
• Garraway SM, Xu Q, Inturrisi CE. siRNA-mediated knockdown of the NR1 subunit gene of the NMDA receptor attenuates formalin-induced pain behaviors in adult rats. J Pain 2009 Apr [PMID: 19185544] (Rat)
• Lim, G et al. Expression of spinal NMDA receptor PKC after chronic morphine is regulated by spinal glucorticoid receptor, J. Neurosci2005. [PMID: 16319314] (ICC/IF, WB, Rat)
• Nagy, J et al. Differential alterations in the expression of NMDA recaptor subunits following chromic ethanol treatment in primary cultures of rat cortical hippocampal neurons. Neurochemistry International, 42(2003):35-43. 2002 [PMID: 12441166]
• Stewart ME. Development of high-throughput, non-invasive behavioural and cognitive tests in mice to uncover new mechanisms of abnormal cognition and behaviour Thesis Jul 10 1905 (Mouse)

Reviews for NMDAR1 Antibody (NB300-118) (14)

Average Rating: 4.2

(Based on 14 reviews)

We have 14 reviews tested in 3 species: Human, Mouse, Rat.

reviewed by: Anonymous WB Mouse 01/31/2018

Summary

• Application: Western Blot
• Sample Tested: Mouse brain
• Species: Mouse
• Lot: BW1017c

reviewed by: Anonymous ICC Mouse 08/04/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Cultured hippocampal neurons
• Species: Mouse
• Lot: BW1016c

reviewed by: Anonymous IP Mouse 08/04/2017

Summary

• Application: Immunoprecipitation
• Sample Tested: Mice Brain Lysate
• Species: Mouse
• Lot: BW1016c

reviewed by: Anonymous WB Human 08/04/2017

Summary

• Application: Western Blot
• Sample Tested: 293T
• Species: Human
• Lot: WB1016c

reviewed by: Anonymous IP Mouse 07/14/2017

Summary

• Application: Immunoprecipitation
• Sample Tested: brain and spinal cord
• Species: Mouse
• Lot: BW1016c

reviewed by: Anonymous ICC Mouse 07/14/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Cortical neurons
• Species: Mouse
• Lot: BW1016c

reviewed by: Anonymous WB Human 07/14/2017

Summary

• Application: Western Blot
• Sample Tested: brain and spinal cord
• Species: Human
• Lot: BW1016c

reviewed by: Janosch Heller ICC Rat 06/27/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: rat cortical culture (3 weeks old)
• Species: Rat

Comments

• Comments: Fixation Solution and Conditions: 4% paraformaldehyde in PBS, 15 minutes RT
  
  Blocking Solution & Duration: 3% BSA in PBS with 0.2% saponin, 30 minutes RT
  
  Primary Antibody Diluent and Dilutions Tested: 1:200 in 1% BSA in PBS with 0.2% saponin, over night 4°C
  
  Secondary Antibody Manufacturer, Host Species, Dilution, & Diluent: Thermo donkey anti-mouse CF568, 1:250 in PBS with 0.2% saponin, 1 hour, RT

reviewed by: Anonymous IF Mouse 11/26/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse neurons
• Species: Mouse

Blocking

• Blocking Details: 1% BSA

Details

• Detection Notes: Confocal microscopy

reviewed by: Anonymous ICC Mouse 11/26/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse neurons
• Species: Mouse
• Lot: BW714r

Blocking

• Blocking Details: 1% BSA for 1 hr

Details

• Detection Notes: Confocal microscopy

reviewed by: Anonymous IP Mouse 10/16/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: Mouse Brain Tissue
• Species: Mouse
• Lot: BW714r

Blocking

• Blocking Details: 5% skimmilk in TBS-T

Details

• Detection Notes: Chemiluminescence detection

reviewed by: Anonymous IP Mouse 10/15/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: Mouse neuronal cultures
• Species: Mouse
• Lot: BW714r

Blocking

• Blocking Details: 5%skimmilk in TBS-T

Details

• Detection Notes: Chemiluminescence

reviewed by: Anonymous IP Mouse 10/15/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: Mouse neurons
• Species: Mouse
• Lot: BW714r

Blocking

• Blocking Details: 5% skimmilk in TBS-T

Details

• Detection Notes: Chemiluminescence, 5 min using West Pico, whole cell lysate as positive and non-immune IgG as negative contril
• Immunoprecipitation Details: Protein G

reviewed by: Fugui Li IF Human 09/30/2014

Summary

• Application: Immunofluorescence
• Sample Tested: NR1 plasmid transfected HEK293T cells
• Species: Human

Bioinformatics

• Gene Symbol: GRIN1
• Entrez:
  • 24408(Rat)
• Uniprot:
  • P35439(Rat)