NMDAR1 Antibody (R1JHL)

Images

 
Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor.
Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - The blots show the examples of immunobands against NMDAR1 and beta-actin antibodies on the membrane, which was performed on the membrane protein prepared from the ...read more
Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - NR1 surface staining of mouse corical neruons. This image was submitted via customer Review.
Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - Mouse neuron stained with anti-NMDAR1 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - Mixed rat cortical cultures (21 DIV) were labelled with anti-NMDAR1 (1:200) and anti-mouse CF568 (1:250). The antibody labels small punctae ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
R1JHL
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

NMDAR1 Antibody (R1JHL) Summary

Immunogen
Fusion protein containing amino acids 1-564 of the NMDAR1 subunit. Accession # P35439
Marker
Neuronal Marker
Specificity
Specific for the ~120k NR1 subunit of the NMDA receptor.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
GRIN1
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1 ug per ml
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 3ug/200ug lysate
  • Western Blot 1:1000
Application Notes
Use in ICC/IF and IHC-F reported in scientific literature (PMID: 20414717). Use in IHC-P reported in scientific literature (PMID 26635527).
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 14 Reviews rated 4.2
using
NB300-118 in the following applications:

Publications
Read Publications using
NB300-118 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 20414717). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Preservative
No Preservative
Concentration
LYOPH
Purity
Tissue culture supernatant
Reconstitution Instructions
Reconstitute with 50 ul PBS to desired concentration.

Alternate Names for NMDAR1 Antibody (R1JHL)

  • glutamate [NMDA] receptor subunit zeta-1
  • glutamate receptor, ionotropic, N-methyl D-aspartate 1
  • NMDA1
  • NMDAR1glutamate [NMDA] receptor subunit zeta 1
  • NMD-R1
  • N-methyl-D-aspartate receptor channel, subunit zeta-1
  • N-methyl-D-aspartate receptor subunit NR1
  • NR1

Background

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NMDAR1 Antibody (NB300-118)(17)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: Flow, ICC/IF, IHC-Fr, IHC-P, KO, WB.


Filter By Application
Flow
(1)
ICC/IF
(2)
IHC-Fr
(1)
IHC-P
(1)
KO
(1)
WB
(10)
All Applications
Filter By Species
Human
(4)
Mouse
(6)
Rat
(7)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB300-118 Applications Species
Wang, Y, Kerrisk Campbell, M Et al. PCDH7 interacts with GluN1 and regulates dendritic spine morphology and synaptic function. Sci Rep Jul 2 2020 [PMID: 32616769] (Flow, Rat)

Details:
Citation using the Alexa Fluor 647 format of this antibody.
Flow Rat
Shi Y, Hung ST, Rocha G et al. Identification and therapeutic rescue of autophagosome and glutamate receptor defects in C9ORF72 and sporadic ALS neurons JCI Insight Jul 16 2019 [PMID: 31310593] (WB, Human) WB Human
Stewart M, Lau P, Banks G et al. Loss of Frrs1l disrupts synaptic AMPA receptor function, and results in neurodevelopmental, motor, cognitive and electrographical abnormalities Dis Model Mech Feb 22 2019 [PMID: 30692144] (WB, Mouse) WB Mouse
Shi Y, Lin S, Staats KA, Li Y. Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons. Nat. Med. 2018 Feb 05 [PMID: 29400714] (Human) Human
Mashkina AP, Cizkova D, Vanicky I et al. NMDA receptors are expressed in lymphocytes activated both in vitro and in vivo. Cell Mol Neurobiol 2010 Aug [PMID: 20414717] (IHC-Fr, ICC/IF, Rat, Human) IHC-Fr, ICC/IF Rat, Human
Rahim M, Yoon Y, Dimovasili C et al. PS1 familial Alzheimer disease mutants inactivate EFNB1- and BDNF-dependent neuroprotection against excitotoxicity by affecting neuroprotective complexes of NMDA receptor Brain Communications Jul 20 2020 (WB, KO, Mouse) WB, KO Mouse
Gorska-Ponikowska M, Perricone U, Kuban-Jankowska A et al. 2-Methoxyestradiol Impacts on Amino Acids-mediated Metabolic Reprogramming in Osteosarcoma Cells by Interaction with NMDA Receptor. J. Cell. Physiol. Mar 6 2017 [PMID: 28262924] (WB, Human) WB Human
Hewett SJ, Shi J, Gong Y et al. Spontaneous Glutamatergic Synaptic Activity Regulates Constitutive COX-2 Expression in Neurons: Opposing roles for the transcription factors CREB and Sp1. J. Biol. Chem. Nov 14 2016 [PMID: 27875294] (WB, Mouse) WB Mouse
Brai E, Marathe S, Astori S et al. Notch1 Regulates Hippocampal Plasticity Through Interaction with the Reelin Pathway, Glutamatergic Transmission and CREB Signaling. Front Cell Neurosci. 2015 Dec 04 [PMID: 26635527] (IHC-P, Mouse) IHC-P Mouse
Plaza-Zabala A, Li X, Milovanovic M et al. An investigation of interactions between hypocretin/orexin signaling and glutamate receptor surface expression in the rat nucleus accumbens under basal conditions. Neurosci Lett. 2013 Dec 17 [PMID: 24262606] (WB, Rat) WB Rat
Show All 17 Publications.

Reviews for NMDAR1 Antibody (NB300-118) (14) 4.214

Average Rating: 4.2
(Based on 14 reviews)
We have 14 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB300-118:
Filter by Applications
Western Blot
(3)
Immunocytochemistry
(4)
Immunoprecipitation
(5)
Immunofluorescence
(2)
All Applications
Filter by Species
Human
(3)
Mouse
(10)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot NMDAR1 NB300-118
Enlarge
4
reviewed by:
MD AL RAHIM
Western Blot Mouse 01/31/2018
View

Summary

ApplicationWestern Blot
Sample TestedMouse brain
SpeciesMouse
LotBW1017c
Immunocytochemistry NMDAR1 NB300-118
Enlarge
4
reviewed by:
MD AL RAHIM
Immunocytochemistry Mouse 08/04/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedCultured hippocampal neurons
SpeciesMouse
LotBW1016c
Immunoprecipitation NMDAR1 NB300-118
Enlarge
4
reviewed by:
MD AL RAHIM
Immunoprecipitation Mouse 08/04/2017
View

Summary

ApplicationImmunoprecipitation
Sample TestedMice Brain Lysate
SpeciesMouse
LotBW1016c
Western Blot NMDAR1 NB300-118
Enlarge
4
reviewed by:
MD AL RAHIM
Western Blot Human 08/04/2017
View

Summary

ApplicationWestern Blot
Sample Tested293T
SpeciesHuman
LotWB1016c
Immunoprecipitation NMDAR1 NB300-118
Enlarge
4
reviewed by:
MD AL RAHIM
Immunoprecipitation Mouse 07/14/2017
View

Summary

ApplicationImmunoprecipitation
Sample Testedbrain and spinal cord
SpeciesMouse
LotBW1016c
Immunocytochemistry NMDAR1 NB300-118
Enlarge
4
reviewed by:
MD AL RAHIM
Immunocytochemistry Mouse 07/14/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedCortical neurons
SpeciesMouse
LotBW1016c
Western Blot NMDAR1 NB300-118
Enlarge
4
reviewed by:
MD AL RAHIM
Western Blot Human 07/14/2017
View

Summary

ApplicationWestern Blot
Sample Testedbrain and spinal cord
SpeciesHuman
LotBW1016c
  3
reviewed by:
Verified Customer
Immunocytochemistry Rat 06/27/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedrat cortical culture (3 weeks old)
SpeciesRat

Comments

CommentsFixation Solution and Conditions: 4% paraformaldehyde in PBS, 15 minutes RT

Blocking Solution & Duration: 3% BSA in PBS with 0.2% saponin, 30 minutes RT

Primary Antibody Diluent and Dilutions Tested: 1:200 in 1% BSA in PBS with 0.2% saponin, over night 4°C

Secondary Antibody Manufacturer, Host Species, Dilution, & Diluent: Thermo donkey anti-mouse CF568, 1:250 in PBS with 0.2% saponin, 1 hour, RT
Immunofluorescence NMDAR1 NB300-118
Enlarge
4
reviewed by:
MD AL RAHIM
Immunofluorescence Mouse 11/26/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse neurons
SpeciesMouse
Immunocytochemistry NMDAR1 NB300-118
Enlarge
4
reviewed by:
MD AL RAHIM
Immunocytochemistry Mouse 11/26/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse neurons
SpeciesMouse
LotBW714r
Immunoprecipitation NMDAR1 NB300-118
Enlarge
5
reviewed by:
MD AL RAHIM
Immunoprecipitation Mouse 10/16/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedMouse Brain Tissue
SpeciesMouse
LotBW714r
Immunoprecipitation NMDAR1 NB300-118
Enlarge
5
reviewed by:
MD AL RAHIM
Immunoprecipitation Mouse 10/15/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedMouse neuronal cultures
SpeciesMouse
LotBW714r
Immunoprecipitation NMDAR1 NB300-118
Enlarge
5
reviewed by:
MD AL RAHIM
Immunoprecipitation Mouse 10/15/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedMouse neurons
SpeciesMouse
LotBW714r
  5
reviewed by:
Verified Customer
Immunofluorescence Human 09/30/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedNR1 plasmid transfected HEK293T cells
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NMDAR1 Antibody (NB300-118) (0)

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Recent Reviews

4.2
5
4
4
9
3
1
2
0
1
0

MD AL RAHIM
01/31/2018
Application: Western Blot
Species: Mouse

MD AL RAHIM
08/04/2017
Application: Immunocytochemistry
Species: Mouse

MD AL RAHIM
08/04/2017
Application: Immunoprecipitation
Species: Mouse

Bioinformatics

Gene Symbol GRIN1
Entrez
Uniprot