NMDAR2B Antibody Summary
Fusion protein from the C-terminus of the NR2B subunit of rat NMDA receptor.
NB300-106 can be used to demonstrate that NR2B is highly enriched in hippocampus compared to either cortex or cerebellum.
Specific for the ~180kDa NR2B subunit of the NMDA receptor. Recognizes human, mouse and rat forms of the NR2B subunits of NMDAR. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody. No reactivity towards the NR2A and NR2C subunits.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 1:1000
- Flow Cytometry 1:10-1:1000
- Immunocytochemistry/Immunofluorescence 1:10-1:500
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:1000-1:2000
- Immunoprecipitation 3 ul per 200 ug lysate
NB300-106 can be used in Western blot where a band is seen at ~ 180 kDa representing the NMDAR2B. Immunohistochemical analysis can be performed using frozen or fixed tissue sections. NB300-106 can also be used to quantitatively immunoprecipitate NMDAR2B where 3 ul will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2B in 200 mg of rat brain. NB300-106 has also been used successfully in immunofluoresence as reported in the literature (PMID: 20082293) and reported by an anonymous customer review. After reconstitution the antibody should be aliquoted and stored at -20C. Adequate amount of material to conduct 10-mini Western Blots.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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NB300-106 in the following applications:
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Immunogen affinity purified
PLEASE NOTE: If 0.01mg size is ordered, product will come lyophilized from 5 mM ammonium bicarbonate. Please reconstitute lyophilized product in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4)
Alternate Names for NMDAR2B Antibody
- glutamate [NMDA] receptor subunit epsilon-2
- glutamate receptor subunit epsilon-2
- glutamate receptor, ionotropic, N-methyl D-aspartate 2B
- N-methyl D-aspartate receptor subtype 2B
- N-methyl-D-aspartate receptor subunit 3
The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
View specific protocols for NMDAR2B Antibody (NB300-106):
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FAQs for NMDAR2B Antibody (NB300-106). (Showing 1 - 1 of 1 FAQs).
Because I'm looking at staining in a region notorious for high background, they want me to do this control. Could you send me the sequence so that I can have the peptide synthesized?
- Unfortunately the sequence for the fusion protein is proprietary.
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