NMDAR2B Antibody

Images

 
Western Blot: NMDAR2B Antibody [NB300-106]
Flow Cytometry: NMDAR2B Antibody [NB300-106] - NMDA2B receptor expression on CD4+ T cells. Image supplied by review from confirmed customer.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

NMDAR2B Antibody Summary

Immunogen
Fusion protein from the C-terminal region of the NR2B subunit
Localization
NB300-106 can be used to demonstrate that NR2B is highly enriched in hippocampus compared to either cortex or cerebellum.
Specificity
Specific for the ~180kDa NR2B subunit of the NMDA receptor. Recognizes human, mouse and rat forms of the NR2B subunits of NMDAR. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody. No reactivity towards the NR2A and NR2C subunits.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GRIN2B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:1000-1:2000
  • Immunoprecipitation 3 ul per 200 ug lysate
Application Notes
NB300-106 can be used in Western blot where a band is seen at ~ 180 kDa representing the NMDAR2B. Immunohistochemical analysis can be performed using frozen or fixed tissue sections. NB300-106 can also be used to quantitatively immunoprecipitate NMDAR2B where 3 ul will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2B in 200 mg of rat brain. NB300-106 has also been used successfully in immunofluoresence as reported in the literature (PMID: 20082293) and reported by an anonymous customer review. After reconstitution the antibody should be aliquoted and stored at -20C. Adequate amount of material to conduct 10-mini Western Blots.
Theoretical MW
180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4
using
NB300-106 in the following applications:

Publications
Read Publications using
NB300-106 in the following applications:

  • 5 publications
  • IHC
    3 publications
  • IP
    2 publications
  • WB
    6 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
0.1 mg/ml
Purity
Immunogen affinity purified

Notes

PLEASE NOTE: If 0.01mg size is ordered, product will come lyophilized from 5 mM ammonium bicarbonate. Please reconstitute lyophilized product in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4)

Alternate Names for NMDAR2B Antibody

  • glutamate [NMDA] receptor subunit epsilon-2
  • glutamate receptor subunit epsilon-2
  • glutamate receptor, ionotropic, N-methyl D-aspartate 2B
  • hNR3
  • MGC142180
  • NMDAR2BMGC142178
  • N-methyl D-aspartate receptor subtype 2B
  • N-methyl-D-aspartate receptor subunit 3
  • NR2B
  • NR3

Background

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-105
Species: Hu, Mu, Rt, Rb
Applications: WB, IHC, IHC-P, IP
NB300-118
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC-Fr, IHC-P, IP, ICC, IF
NBP1-88790
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB300-107
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IP
NBP1-88191
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-64698
Species: Hu
NB300-556
Species: Hu, Mu, Rt, In, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, ICC
248-BD
Species: Hu, Mu, Rt, Ca, Eq
NB300-124
Species: Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr
NB600-101
Species: Hu, Mu, Rt, Bv, Ha, Pm
Applications: WB, Simple Western, DB, EM, ICC/IF, IHC, IHC-P, IP, PA, B/N
H00005662-B01P
Species: Hu, Rt
Applications: WB, ICC/IF
NBP1-84212
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB500-517
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
AF2685
Species: Hu
Applications: WB, Simple Western, IHC

Publications for NMDAR2B Antibody (NB300-106)(11)

We have publications tested in 3 confirmed species: Mouse, Rat, Rat .

We have publications tested in 4 applications: ICC/IF, IHC, IP, WB.


Filter By Application
ICC/IF
(5)
IHC
(3)
IP
(2)
WB
(6)
All Applications
Filter By Species
Mouse
(2)
Rat
(7)
Rat
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB300-106 Applications Species
Kermath BA, Riha PD, Sajjad A, Gore AC. Effects of Chronic NMDA-NR2b Inhibition in the Median Eminence of the Reproductive Senescent Female Rat. J Neuroendocrinol. 2013 Oct [PMID: 23957788] (IHC, ICC/IF, Rat) IHC, ICC/IF Rat
Tse YC, Bagot RC, Hutter JA et al. Modulation of Synaptic Plasticity by Stress Hormone Associates with Plastic Alteration of Synaptic NMDA Receptor in the Adult Hippocampus. PLoS ONE 20116(11). [PMID: 22069501] (WB, Rat ) WB Rat
Tao P-L, Chen C-F, Huang EY-K. Dextromethorphan attenuated the higher vulnerability to inflammatory thermal hyperalgesia caused by prenatal morphine exposure in rat offspring. J Biomed Sci;18(1):64. 2011 Aug 23. [PMID: 21861871] (WB, Rat) WB Rat
Snyder MA, Cooke BM, Woolley CS. Estradiol potentiation of NR2B-dependent EPSCs is not due to changes in NR2B protein expression or phosphorylation Hippocampus 2011 Apr [PMID: 20082293] (IHC, ICC/IF, WB, Rat) IHC, ICC/IF, WB Rat
Maffucci JA, Noel ML, Gillette R et al. Age- and hormone-regulation of N-methyl-D-aspartate receptor subunit NR2b in the anteroventral periventricular nucleus of the female rat: implications for reproductive senescence. J Neuroendocrinol 2009 May [PMID: 19302193] (IHC, ICC/IF, Rat) IHC, ICC/IF Rat
Li L, El-Hayek YH, Liu B et al. Direct-current Electrical Field Guides Neuronal Stem/progenitor Cell Migration. Stem Cells : 2007-1022. 2008 [PMID: 18556511] (ICC/IF, IP, Rat) ICC/IF, IP Rat
Park CS, Elgersma Y, Grant SG et al. alpha-Isoform of calcium-calmodulin-dependent protein kinase II and postsynaptic density protein 95 differentially regulate synaptic expression of NR2A- and NR2B-containing N-methyl-d-aspartate receptors in hippocampus. Neuroscience 2008 Jan 2 [PMID: 18082335] (WB, Rat) WB Rat
Head BP, Patel HH, Tsutsumi YM et al. Caveolin-1 expression is essential for N-methyl-D-aspartate receptor-mediated Src and extracellular signal-regulated kinase 1/2 activation and protection of primary neurons from ischemic cell death. FASEB J. 2008 Mar [PMID: 17905724] (ICC/IF, Mouse)

Details:
IF/ICC: Cultured mouse cortical neurons, Fig 2.
ICC/IF Mouse
Gabra, Bichoy H et al. Decrease in N-Methyl-D-aspartic Acid Receptor-NR2B Subunit Levels by Intrathecal Short-Hairpin RNA Blocks Group I Metabotropic Glutamate Receptor-Mediated Hyperalgesia. J Pharmacol. Exp. Ther. 322: 186-194. 2007 [PMID: 17405869] (WB, Mouse) WB Mouse
Ning, K et al. Dual Neuroprotective Signaling Mediated by Downregulating Two Distinct Phosphatase Activities of PTEN. The Journal of Neuroscience; 24: 4052-4060. 2004 [PMID: 15102920] (WB, IP, Rat) WB, IP Rat
Show All 11 Publications.

Reviews for NMDAR2B Antibody (NB300-106) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Mouse, Other.

Reviews using NB300-106:
Filter by Applications
Flow
(1)
WB
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry NMDAR2B NB300-106
Enlarge
reviewed by:
Anonymous
Flow 01/31/2014
View

Summary

ApplicationFlow Cytometry
LotCS212R
 
reviewed by:
Samantha Garcia
WB Mouse 11/06/2013
View

Summary

ApplicationWestern Blot
Sample TestedMouse brain synaptosomal membrane
SpeciesMouse
Lotcvp110

Blocking

Blocking DetailsTBST with 1% milk

Primary Anitbody

Dilution Ratio1:200 in blocking buffer
 
reviewed by:
Anonymous
ICC Other 03/23/2010
View

Summary

ApplicationImmunocytochemistry
Sample TestedRat brain tissue
SpeciesOther
Lotcvp608
Commentsgranule cell layer of the hippocampus

Blocking

Blocking DetailsBlocking Buffer: Normal Donkey serum, Blocking Time: 30 minutes, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/150, Primary Ab Incubation Time: 3 days, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab Dilution: 1/1000

Comments

Commentsgranule cell layer of the hippocampus

Product General Protocols

View specific protocols for NMDAR2B Antibody (NB300-106): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NMDAR2B Antibody (NB300-106). (Showing 1 - 1 of 1 FAQs).

  1. Because I'm looking at staining in a region notorious for high background, they want me to do this control. Could you send me the sequence so that I can have the peptide synthesized?
    • Unfortunately the sequence for the fusion protein is proprietary.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for NMDAR2B Antibody (NB300-106)

Discover related pathways, diseases and genes to NMDAR2B Antibody (NB300-106). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NMDAR2B Antibody (NB300-106)

Discover more about diseases related to NMDAR2B Antibody (NB300-106).
 

Pathways for NMDAR2B Antibody (NB300-106)

View related products by pathway.

PTMs for NMDAR2B Antibody (NB300-106)

Learn more about PTMs related to NMDAR2B Antibody (NB300-106).
 

Research Areas for NMDAR2B Antibody (NB300-106)

Find related products by research area.

Blogs on NMDAR2B

There are no specific blogs for NMDAR2B, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
1
4
1
3
1
2
0
1
0

Anonymous
01/31/2014
Application: Flow
Species:

 
Samantha Garcia
11/06/2013
Application: WB
Species: Mouse

 
Anonymous
03/23/2010
Application: ICC
Species: Other

Bioinformatics

Gene Symbol GRIN2B
Entrez
Uniprot